<周易, 繫辭傳,上> 考釋

金世煥 부산대학교 인문학연구소 1999 人文論叢 Vol.54 No.-

레미콘 회수수의 안정적 재활용을 위한 교반수조의 설비유형 효과

이문환,이세현,박영신,박재명 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.2

Using the sludge water in producing ready mixed concrete, which is generated as washing surplus concrete after completing manufacturing and transportation of concrete is the best method in meeting the issue of cost of production and environment. However, in reality, using sludge water decreases Quality, and solid ingredients adhere to the transportation line of sludge water and the basin of sludge water, agitated to prevent precipitation of sludge, which incurs tremendous amount of repair expenses. In order to resolve the problems above, we have studied stable methods of supplying sludge water and experimentally reviewed the types of facilities. The result showed that a facility that added stabilizing agents which retarded hydration of unhydrated cement in sludge water for certain periods (2-3 days) and simultaneously agitated and moved by air diffusion and vertical flat blade impeller was most effective in stabilizing concentration of solid and preventing precipitation of solid in the bottom of basin. In addition, the performance of agitation and prevention of adherence of reaction basin of sludge water depended on selective efficiency of sand grain, and it has been studied that buffering inconsistent band of circulation of sludge water by operating the ring blower for air diffusion to the maximum was advantageous to preventing managing concentration and prevention of adherence.

Ficoll을 이용한 닭 염색체 분리 기법에 관한 연구

손시환,류은경 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

본 연구는 닭의 혈액을 이용한 염색체의 분리 방법을 개선하고 자 ficoll을 이용한 순수 백혈구의 분리방법을 개발하고 한편 정밀 핵형 분석을 위한 고해상 염색체를 유기할 수 있는 새로운 배양방법도 제시하였다. 시험 분석은 실용산란계(ISA brown C. C.) 암,수 50개체의 혈액으로부터 백혈구 배양법에 의한 염색체 분리를 수행하고 관행적인 GTG-banding과 high resolution banding을 실시하였다. 닭의 혈액으로 부터 염색체 분리는 ficoll을 이용한 순수 백혈구 분리법을 응용 적용함으로서 중기상의 획득이 획기적으로 개선되었고, 관행적 방법에 비해 월등히 높은 분열지수(mitotic index)를 얻을 수 있었다. 또한 이를 이용한 GTG-banding과 high resolution banding으로서 공히 각 상동염색체별 일관성이 있는 명확하고 특징적 band양상을 얻을 수 있었다. high resolution banding에 의한 대형염색체들에 대한 평균 출현 band수는 150여개로서 관행적인 GTG-banding에 의한 80개의 band보다는 약 70여개정도 많은 band양상을 보였다. 또한 출현 band수의 범위도 GTG-banding의 경우 분석된 염색체간에 다소 큰 변이를 보이나, high resolution banding의 경우 세포들간의 동기화가 이루어져 균일한 양상의 band수를 나타내었다. 따라서 닭에 있어 ficoll처리에 의한 high resolution banding법은 기존 GTG-banding법을 대신할 뿐만 아니라 분자유전학적 연구 등을 위한 보다 정밀한 염색체 표지로서의 역할과 개선된 핵형분석의 자료로서 활용이 가능하리라 사료된다. This study was carried out to develop the method of chromosome preparation from chick blood culture and to identify chick chromosomal mark. Using the ficoll treatment in lymphocyte purification and application of high resolution banding technique in culture, a success result was obtained. The chromosomes were prepared from this culture method with 50 commercial chicks and then we analyzed GTG-banding and high resolution banding. The yield of metaphase spreads and percentage of mitotic index were improved by using of ficoll treatment in lymphocyte culture. The characteristic and distinctive patterns of GTG-band and high resolution band were obtained on each chromosome. The number of discernible bands on macrochromosomes was approximately 150 bands in high resolution banding and 80 bands in GTG-banding. The number of analyzed bands was much more variable in GTG-banding than in high resolution banding. This results implied that the growth cells were synchronized by high resolution banding treatment. Contrasting with a habitual GTG-banding in chicken chromosomes, the ficoll treatment and high resolution banding technique are very useful skill for advanced karyotype analysis and help more precise designation for molecular works.

여성형 유방과 남성유방암에서 HER-2/neu, ER 및 PR의 발현 양상

김세환,김의환 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

To observe the expression of HER-2/neu, ER and PR in breast lesions, such as 14 cases of Gynecornastia, 1 case of invasive papillary carcinoma and 1 case of invasive ductal carcinoma in man and to evaluate the correlation beween results, the authors has been carried out immunohistochemical staining with monoclonal antibodies to HER-2/neu, ER and PR. So, following results are obtained. 1. In Gynecomastia, the HER-2/neu showed weaky positive reactino (score 1) in florid area, but negative reaction in fibrotic area. 2. In Gyneomastia, ER showed 50% positive reaction and PR show 87% positve reaction both in florid and fibrotic area. The ER positive case did not observed among PR negative cases. 3. In male breast carcinoma, the HER-2/neu showed score 3 regardless of cancer type. 4. In male breast carcinoma, the expression pattern of ER and PR show stronger in invastive papillary carcinoma than invasive ductal carcinoma.

포유류의 초기 배자를 이용한 염색체 분석에 관한 연구

손시환,문점동,김기원 진주산업대학교 농업기술연구소 1997 農業技術硏究所報 Vol.10 No.-

초기배자로부터 분할란의 성(性) 감별과 분할배의 발생 성공은 성의 식별과 더불어 유전적으로 우수한 개체의 생산이 가능함을 의미한다. 따라서 본 연구에서는 새앙쥐(mouse) 및 소의 초기배자 상태의 수정란을 이용한 염색체 분석 기술을 개발하고, 각 수정란의 성의 판별과 이들의 유전적 특성을 구명하고자 하였다. 초기 배자를 이용한 염색체 분리의 최적 조건을 결정하기 위하여 colcemid의 농도, colcemid의 처리시간 및 공시된 배자의 발생단계별 분석을 새앙쥐의 배자로서 수행하였다. 분석 결과 염색체 분리에 있어 colcemid의 처리 시간이 유의적인 차이를 보인 반면 다른 요인들 간에는 별다른 차이가 없는 것으로 나타났다. 소의 체외수정란을 이용한 염색체 분석에서는 공시된 수정란의 93%이상에서 증기상의 유도가 가능하였고, 이들 중 65%에서 성의 식별이 가능하였다. 또한 수정란의 분석된 성비는 1:1로서 자연적 성비와 일치하였다. 염색체 분석에 의한 배자의 조기성 감별은 처리 과정상의 기술적 요인들에 의해 매우 큰 영향을 받는데 특히 hypotonic의 처리방법 및 fixation의 방법을 적절히 처리하므로서 효율적이고 높은 성공률을 기대할 수 있다. Chromosome analysis from biopsied or splitted embryos which were fertilized in uitro or in vivo implicates a possibility of production of the genetical superior animals and sex controlled individuals. Therefore, this study was carried out to develop the technical method for chromosome preparation from early bovine and mouse embryos and to identify their sex and genetic characteristics. To determine optimum condition of chromosome preparation, the several factors which include the concentration of colcemid, the duration of culture and embryonic stage were analyzed from mouse embryos. According to analysis, the duration of culture had significantly influence on chromosome preparation. The other factors were not significantly difference among the treatments. In the results of chromosome preparation from intact bovine embryos, the rate of embryos with one or more metaphase plates was 93%, and of embryos with sex analyzable metaphase chromosomes are 65%. Sex ratio of male and female was approximatly 1:1 same as natural sex ratio. The inducing rate of analyzable metaphase was mainly due to technical aspects, expecially hypotonic treatment and fixation.

그리드 연관성을 이용한 지능로봇용 환경 지도 형상의 신뢰성 향상에 관한 연구

임종환,이세진,강철웅 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.2

This paper addresses a new feature map building method that can minimizes the appearance of phantom features by using only sparse sonar data. The approach is composed of extraction of features and building a probability grid map using only the footprint of sparse sonar data, estimation of position uncertainty of the feature, and evaluation of the reliable features. A virtual circle association frame model has been developed, which associates two sonar footprints into a virtual circle frame. Using this model, geometric primitives such as line, point, or arc features are separately extracted. While extracting the feature, a grid map is also built using the orientation probability approach. Position uncertainty of each extracted feature is, then, estimated by considering both the position uncertainty of the robot and the measurement uncertainty of the sonar sensor. Finally, the reliable features among all extracted ones are evaluated from grid association method. The proposed methods have been tested in a real home environment with a mobile robot.

籠球選手 疲勞回復을 위한 魚腥草 服用의 檢證 : 高校 男女 選手를 中心으로

이윤근,권세환 중앙대학교 스포츠과학연구소 2000 體育硏究 Vol.- No.13

It is considered the most of sport drinks that the lack of a herb medicine. We tried to verify of a baseball plyer's the recover from fatigue through the taking an'u sung cho' which have great an effects in the internals. It was based on the fatigue survey report by subjective symptoms that an object to the high school baseball plyer(man:26 woman:24). 1. After taking that, it is showed to helpful the function of the respiratory organs, the muscular system, a joint, recover from fatigue. 2. It is good for mental state, nerve system that has nothing to do with ability to carry out directly, so it served keep a balance and comfortable mental state. 3. A period of 1 month, it is great an effects physically, mentally, nerve system add to other things about the recover from fatigue. 4. It should required to be in the spotlight as a sport drink thet 'u sung cho' follow-up the study of medically, nutritionally.

재생골재 생산방식의 차이에 따른 재생 굵은골재의 품질 특성에 관한 비교 연구

심종우,이문환,이세현,전현규 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The purpose of this study is intended for effective recycling of waste concrete. It analyzes the quality 0 the recycled coarse aggregate with production types. The conclusions of the study are as follows. 1. The recycled coarse aggregate which is produced through 5th Crushing Process (A-Type) shows the improvement in grading and particle shape, reduction in an absorption rate until 1.14% due to removal of attached the mortar and paste. Therefore, it satisfies the standards of KS F 2573(recycled aggregate for concrete) as the first grade. 2. The recycled coarse aggregate which is produced through 3rd Crushing Process(B-Type) is suited to the quality prOVlSlOns of the standards of KS F 2573, shows a 3.12% absorption rate that is second grade as recycled coarse aggregate. Based on the above results, it is possible to product first and second grade recycled coarse aggregates with production types. It is concluded that recycled coarse aggregate with production types can be used as aggregate within required conditions. And more researches are also needed to make recycled coarse aggregate better to use for concrete.

회수수 안정화제를 사용한 콘크리트의 공학적 특성에 관한 연구

김기정,金光華,이문환,이세현,한천구 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

In this study is investigated the engineering properties of concrete using stabilizing agent of recycling water in order to suggest a practical use of recycling water. According to the results. as stabilizing agent is added. fluidity and air content varies slightly with W/C, but does not make a big difference. Bleeding is reduced by adding stabilizing agent, and so it decreases by about 40% at the adding ratio of 0.15%. When stabilizing agent is not added, compressive strength decreases in comparison with plain concrete, but increases by adding stabilizing agent, so it is largest at adding ratio of 0.30%(W/C 30%) and 0.15%(W/C 40, 50%). Length change by drying shrinkage is larger than plain concrete in the case of no adding of stabilizing agent, but decreases by the use of stabilizing agent. Therefore, it is thought that when stabilizing agent is added to recycling water, quality deterioration of concrete is prevented, moreover, quality of concrete can be improved.

안정화제 처리된 회수수를 사용한 콘크리트의 특성에 관한 실험적 연구

박영신,박재명,이세현,이문환 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

Recently, Most enterprises produced ready mixed concrete uses a mixing water as a recycling water without a density control in proportion to guide and standard. That is a cause of deterioration in the concrete quality like a reduce of strength, laitance, crack, etc. This study shows basic materials for development of ready mixed concrete recycling water density control system that holds down a deterioration in the concrete quality by using a mixed water treated recycling water with stabilizing agent and examines a possibility of re-using components of cement in recycling water. Therefore, in the experiment, we make a recycling water contains 3,5,7% of solid content ratio examines a property of a mixing water with a stabilizing agent and without it. Results of this study is next as follow. 1. The slump is decreased 1~2cm in a mixing without a stabilizing agent and the mixing with a stabilizing agent is similar to the standard mixing. 2. The air content is 4~4.7% in proportion to an increase of solid content without a stabilizing agent and the mixing with a stabilizing agent is similar to the standard mixing. 3. The compressive strength of an early curing age is tendency to increase both the mixing without a stabilizing agent and the mixing with a stabilizing agent. But, After 14days is passed, Compressive strength is decreased in a mixing without a stabilizing agent but increased a mixing with a stabilizing agent.