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Effect of sugar contents on choosing the host by Lycorma delicatula (Hemiptera: Fulgoridae)
Sang-Rae Moon,Jeong-Oh Yang,Changmann Yoon,Gil-Hah Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10
This study was performed to analyze the sugar contents from six kinds of plant and investigate their effect on the life span of ggot-mae-mi, Lycorma delicatula. Part of plants were methanol extracted from host plants such as Ailanthus altissima, Vitis vinifera and non-host plants such as Malus pumila, Pyrus calleryana, Hibiscus syriacus, and Pinus densiflora, and concentrated the water layer and then analyzed the sugar contents using HPLC. Ailanthus altissima existed high in sugar contents and followed by Fructose>Glucose, Vitis vinifera was analyzed by an order of Glucose>Fructose>Maltose>Sucrose>Rhamnose; Malus pumila was as Glucose>Fructose; Pyrus calleryana was as Glucose>Unknown>Fructose; Hibiscus syriacus was as Sucrose>Glucose; Pinus densiflora was as Fructose>Glucose>Sucrose. A parafilm bioassay was used to investigate the longevity of L. delicatula nymphs to the sugar contents. Nymphs of L. delicatula was lived as long as 13.1 days in 5% Sucrose solution, but lived as short as 6.0 days in 5% Glucose solution. When provided with only water, L. delicatula lived for 5.4 days. Life span to the each sugar contents were longer lived in an order of Sucrose>Fructose>Rhamnose>Maltose>Glucose. As investigated the life span of L. delicatula nymph according to the combination of sugar contents founded in original plants were lived longer in 5% sugar combination solution of Ailanthus altissima. Analyzed original sugar contents from Malus pumila, Pyrus calleryana, L. delicatula was lived as 7.8, and 7.1 days, respectively, comparing to the Hibiscus syriacus, and Pinus densiflora lived as 6.0, and 4.7 days, respectively. This result were judged that sugar contents affected on choosing the host plants.
Sang-Rae Moon,Jin-Won Jeong,Yun-Ho Shin,Gil-Hah Kim 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10
This study was performed to investigate attraction effect of spearmint oil and to confirm their EAG response against lantern fly, Lycorma delicatula nymphs and adult. In dose responses to spearmint oil, 2nd ∼ 4th nymphs and adult were significantly attracted at a dose of 5㎕. Attraction effect was showed highest (84.8%) at a 4th instar nymph, and mild effect (approximately 60%) was observed at 2nd∼3rd instar nymphs, but the others are not showed the significance. At a dose of 10㎕, 4th instar nymph and adult were significant and only 4th instar nymph was significantly attracted at a dose of 2.5㎕ but the others are not. Carvone, a constituent of spearmint oil, exhibited significantly attraction effect on nymphs and adult except 1st and 2nd instar nymphs. But limonene did not show any attraction effect. The attraction assay of L. delicatula to the mixtures of constituents appeared to be efficient additively. In EAG response to spearmint oil which exhibited attraction effect, antennae of 4th instar nymph and adult responded to only carvone. In conclusion, attraction effect of spearmint oil was more effective in 4th instar nymph and adult than 1st ∼ 3rd instar nymphs.
Endogenous Cushing`s syndrome in a patient with systemic lupus erythematosus
( Sang Mi Ro ),( Su-jin Moon ),( Kyung Ho Moon ),( Deok-jae Han ),( Jain Lee ),( Jin Kyeong Shin ),( Jang-won Son ),( Sung-rae Kim ),( Jun-ki Min ),( Soon Jib Yoo ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Introduction: Systemic lupus erythematosus is one of the autoimmune disease which glucocorticoids are mainstay of treatment. Cushing’s syndrome caused by glucocorticoid excess which could be exogenous or endogenous. Although iatrogenic Cushing’s syndrome is most common form especially in the patient with glucocorticoid treatment, endogenous glucocorticoid excess should be under consideration because of different treatment strategy. Case Report: We describe a 51-year old woman had had a longstanding history of SLE. She was treated with steroid and cytoxan pulse therapy and plasmapheresis. Her lupus activity had remained stable for 7 years with low-dose glucocorticoid treatment. She showed excessive weight gain, easy bruising, moon face, truncal obesity, acne, and menstrual disorder. With history of a steroid therapy, iatrogenic Cushing’s syndrome was considered by priority. However endogenous Cushing’s syndrome was suspected in respect that the feature of Cushing’s syndrome had been aggravated with the long term use of minimal dose glucocorticoid. After evaluation under the impression of Cushing’s syndrome we diagnosed her as concomitant endogenous Cushing’s syndrome due to a left adrenal adenoma.(table1)The patient underwent laparoscopic left adrenalectomy. SLE was controlled with transient apply of low-dose glucocorticoid treatment and her lupus activity remains stable without glucocorticoid treatment until now. Discussions: This is the first reported case of concomitant endogenous Cushing’s syndrome onset with preexisting SLE in Korea. This case shows the importance of differential diagnosis of exogenous Cushing’s syndrome and endogenous Cushing’s syndrome in autoimmune disease patients with glucocorticoid therapy.
Sang Un Kim,Kyeong Gon Moon,In Seok Eom,Young Deok Bae,Young Guk Kim,Il Rae Rho 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.10
Background : This study was carried out to select the medicinal crop having the superior antimicrobial activity against 5 microorganisms among 20 medicinal crops. Methods and Results : Dried plants were extracted by methanol. The extracts of each plant were evaporated under vacuum. The extracts were absorbed into 50㎕ of each extracts on paper disk and diameter of clear zone was confirmed around the colony. The results showed that diameter of the clear zone against Escherichia coli was the widest in Rhus javanica(R. javanica, 16mm), followed by Evening primrose(14.5mm), Chinaberry(13mm) and Giant knotweed(11mm). Also, diameter of the clear zone against Vibrio parahaemolyticu was the widest in R. javanica(26mm), followed by Evening primrose(21mm), chrysanthemum flower(19mm), Chinese pepper(16.5mm), Chidii rhizoma(11.5mm), Curly dock(11.5mm), Comfrey(11mm), Black nightshade(11mm) and Mustard(10mm). Likewise, diameter of the clear zone aginast Proteus mirabilis was the widest in R. javanica(11.5mm), followed by Chinaberry(11mm). Specially, diameter of the clear zone against Bacillus subtilis and Staphylococcus aureus was identified only in R. javanica(12mm and 11mm, respectively). Therefore, R. javanica showed the best excellent antimicrobial activity among 20 medicinal crops. Total phenolic and flavonoid contents of R. javanica were 2,725mg/100g and 30.2mg/100g respectively. DPPH and ABTS activity of R. javanica were 92.6% and 98.4%, respectively. Conclusion : These results suggest that R. javanica can be utilized as natural food additives and antioxidant materials because of its powerful antimicrobial action and the high antioxidant activity.
( Sang Rae Moon ),( Sun Ran Cho ),( Jin Won Jeong ),( Young Ho Shin ),( Jeong Oh Yang ),( Ki Su Ahn ),( Chang Mann Yoon ),( Gil Hah Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Attraction responses of plant essential oils were investigated, and the electrophysiological response to nymphs and adults of spot clothing wax cicada (Lycorma delicatula) was confirmed. Of the ten tested oils, only spearmint oil was found attractive. In dose responses of spearmint oil, second to fourth instar nymphs, as well as adults, were significantly attracted to a dose of 5 μL; for nymphs, fourth instar nymph showed greatest attraction response (90.9%), and second and third instar nymphs showed mild attraction. At a dose of 10 μL, fourth instar nymphs and adults were significantly attracted to spearmint oil. Only fourth instar nymphs were attracted to spearmint oil at 2.5 μL. After analyzing spearmint oil using gas chromatography (GC) and GC/mass spectrometry, carvone constituent was found as a significant attractant for both nymphs and adults, except for first instar nymphs. Limonene did not show any attraction response. All constituents mixed with each other appeared to have an additive effect. In electrophysiological response to spearmint oil, antennae of only fourth instar nymphs and female adults responded to carvone. Therefore, spearmint oil may be effective as an attractant for control of L. delicatula populations. In a field test, fourth nymphs and female adults were highly attracted to 20 μL of spearmint oil. This is the first report on attraction response of L. delicatula to spearmint oil in the laboratory and the field.
Sang-Rae Moon,Sun-Ran Cho,Jin-Won Jeong,Youn-Ho Shin,Jeong-Oh Yang,Ki-Su Ahn,Changmann Yoon,Gil-Hah Kim 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Attraction responses of plant essential oils were investigated, and the electrophysiological response to nymphs and adults of spot clothing wax cicada (Lycorma delicatula) was confirmed. Of the ten tested oils, only spearmint oil was found attractive. In dose responses of spearmint oil, second to fourth instar nymphs, as well as adults, were significantly attracted to a dose of 5 μL; for nymphs, fourth instar nymph showed greatest attraction response (90.9%), and second and third instar nymphs showed mild attraction. At a dose of 10 μL, fourth instar nymphs and adults were significantly attracted to spearmint oil. Only fourth instar nymphs were attracted to spearmint oil at 2.5 μL. After analyzing spearmint oil using gas chromatography (GC) and GC/mass spectrometry, carvone constituent was found as a significant attractant for both nymphs and adults, except for first instar nymphs. Limonene did not show any attraction response. All constituents mixed with each other appeared to have an additive effect. In electrophysiological response to spearmint oil, antennae of only fourth instar nymphs and female adults responded to carvone. Therefore, spearmint oil may be effective as an attractant for control of L. delicatula populations. In a field test, fourth nymphs and female adults were highly attracted to 20 μL of spearmint oil. This is the first report on attraction response of L. delicatula to spearmint oil in the laboratory and the field.
Sang-Rae Moon,Doo-Jin Noh,Jeong-Oh Yang,Changmann Yoon,Ki-Su Ahn,Gil-Hah Kim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05
This study was performed to investigate the seasonal occurrence, developmental characteristics of each nymphal stages with different temperatures (20, 25, 30℃), longevity and fecundity of ussur brown katydid, Paratlanticus ussuriensis, damaging by outbreaks in the orchard areas of Bitan-ri, Yeongdong, Chungbuk. Paratlanticus ussuriensis occurred from late-March to late-August with peak of mid-May. Newly emerged nymphs appeared from March and do damaged fruit orchards with peak of mid-May when P. ussuriensis existed as 4th and 5th nymphal stages. P. ussuriensis adult occurred from early-June to mid-Aug. with peak of mid-July. Total density of P. ussuriensis was showed highest in mid-May. P. ussuriensis goes through nymphal stages to 7th nymph, the ovipositor began exposed to outside from the 4th instar and the body weight increased heavily from this stage and the wings were observed from 6th instar. Developmental period was longer as increased the nymphal stages. Sex ratio of collected insect was showed as 0.57; females more than males. As increased the temperature, developmental period was to be short. Preoviposition was also to be short as 5.0, 4.3, and 3.4 days at 20, 25, 30℃, respectively, and fecundity increased as 69.0, 87.1, and 104.3 at 20, 25, 30℃, respectively. Longevity of male and female at 25℃ was showed the longest with 35.7, and 32.9 days and showed the shortest with 30.1 and 28.1 days at 30℃, respectively. The difference of developmental period in male and female were showed longer in female without relation of temperature. The eggs laid were frequently distributed 3 to 4 cm from soil surface, and showed the behavior laying eggs intensively when early oviposition period.