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Sun-Ran Cho,Youn-Ho Shin,Hyun-Na Koo,Gil-Hah Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
This study was performed to investigate the effect of flonicamid and thiamethoxam treated at sublethal concentration (LC10, LC30) on development period, adult longevity and fecundity and the feeding behaviour of Myzus persicae adult. Developmental period of M. Persicae nymph took 5.9 days in LC10, and 6.1 days in LC30 in both insecticides, comparing with control (5.7 days), it showed longer than those of the control, but there was no significance. Adult longevity treated at LC10 and LC30 of flonicamid was showed 13.2 and 13.7 days, respectively, and LC10 of thiamethoxam was examined as 14.7 days, it showed longer than control of 11.6 days. Mean daily fecundity exhibited higher in LC10 (3.1) and LC30 (3.1) of flonicamid than that of control (2.5), but thiamethoxam are not. Total fecundity exhibited higher in LC10 (41.8) and LC30 (43.0) of flonicamid, in LC10 (42.1) of thiamethoxam than that of control (29.5). Feeding behavior was examined using EPG (electrical penetration graph). EPG data indicated that flonicamid and thiamethoxam increased the duration of non-probing periods and decreased the duration of phloem ingestion.
Sun-Ran Cho,Jin-Won Jeong,Youn-Ho Shin,Young-Uk Park,Seung-Hwan Yun,Changmann Yoon,Hyun-Na Koo,Gil-Hah Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05
In this study, we compared global proteome profiles and the expression pattern of defense-related genes in Chinese cabbage when infested by Myzus persicae and Plutella xylostella. Four-week-old Chinese cabbage was exposed to each insect for 24 h, and then proteins and total RNA were extracted from leaves. To elucidate the herbivore-induced differentially expressed proteins in Chinese cabbage, proteins were separated by two-dimensional gel electrophoresis, and visualized by staining with Coomassie G250. Approximately 1600 protein spots were separated and 249 protein spots showed reproducible changes in expression. Among them, nine proteins whose expressions were markedly up-regulated in M. persicae-infested group were identified using matrixassisted laser desorption/ionization time of flight mass spectrometry. The identified herbivore-responsive proteins (ribulose-1,5-bisphosphate carboxylase/ oxygenase, ATP synthase CF1, putative mismatch binding protein Mus3, and integrase core domain-containing protein) were involved in regulation of photosynthesis, carbohydrate metabolism and DNA repair. The expression levels of chitinase, b-1,3-glucanse, peroxidase, PR1, and PR4 in herbivore-infested Chinese cabbage were analyzed by reverse transcription-polymerase chain reaction. The results clarify the response of Chinese cabbage to two herbivore attack at the protein level.
Cho, Hea-Young,Kang, Hyun-Ah,Park, Chan-Ho,Kim, Se-Mi,Kim, Dong-Ho,Park, Sun-Ae,Kim, Kyung-Ran,Hur, Hyeon,Lee, Yong-Bok The Korean Society of Pharmaceutical Sciences and 2005 Journal of Pharmaceutical Investigation Vol.35 No.5
The purpose of the present study was to evaluate the bioequivalence of two torasemide tablets, Torem tablet (Roche Korea Co., Ltd., Korea, reference drug) and Boryung Torsemide tablet (Boryung Pharmaceutical Co., Ltd., Korea, test drug), according to the guidelines of Korea Food and Drug Administration (KFDA). After adding an internal standard (furosemide) to human serum, serum samples were extracted using 5 mL of ethyl acetate. Compounds were analyzed by reverse-phase HPLC method with UV detection. This method showed linear response over the concentration range of 0.05 ug/mL with correlation coefficient of 0.999. The lower limit of quantitation using 0.5 mL of serum was 0.05 ug/mL which was sensitive enough for pharmacokinetic studies. Twenty-eight healthy male Korean volunteers received each medicine at the torasemide dose of 20 mg in a $2{\times}2$ crossover study. There was a one-week washout period between the doses. Serum concentrations of torasemide were monitored by an HPLC-UV for over a period of 12 hr after the administration. $AUC_{t}$(the area under the serum concentration-time curve from time zero to 12 hr) was calculated by the linear trapezoidal rule method. $C_{max}$ (maximum serum drug concentration) and $T_{max}$ (time to reach $C_{max}$) were compiled from the serum concentration-time data. Analysis of variance was carried out using logarithmically transformed $AUC_{t}$ and $C_{max}$. No significant sequence effect was found for all of the bioavailability parameters indicating that the crossover design was properly performed. The 90% confidence intervals of the $AUC_{t}$ ratio and the $C_{max}$ ratio for Boryung Torsemide/Torem were log 0.97-10g 1.03 and log 0.93log 1.12, respectively. These values were within the acceptable bioequivalence intervals of log 0.80-log 1.25. Thus, the criteria of the KFDA guidelines for the bioequivalence was satisfied, indicating Boryung Torsemide tablet and Torem tablet are bioequivalent.