Production of Pectic Enzymes and Its Properties for Citrus Wine Making

Koh,Jeong-sam,Ko,Tae-am,Kang,Sang-bum,Kang,Soon-seon 濟州大學校 亞熱帶農業硏究所 1986 亞熱帶農業硏究 Vol.3 No.-

투명하고 방향성이 좋은 감귤발효주를 생산하기 위하여, 감귤중에 함유하고 있는 고분자물질을 분해할 수 있는 효소생산성 유용균주를 제주도의 토양중에서 분리 선발하였으며, 이 균주가 생산하는 효소의 생산성 및 효소의 특성과 감귤발효주 제조중에 처리하여 그의 효과를 검토하였다. 선발균주는 형태학적 제특성을 검토한 결과 Aspergillus niger CCM-4로 동정하였다. 밀기울 또는 감귤피를 탄소원으로한 고체배양에서 30℃, 3일간 배양하므로써 펙틴분해효소의 생산이 가능하였으며, 배지의 pH와 그외의 요인등은 효소생산에 큰 영향을 주지 않았다. 조효소액의 반응최적조건은 pH 4.0, 온도 40℃였으며, 50℃에서 1시간처리 후에도 효소의 안정성을 유지하고 있었다. 조효소를 감귤발효주 제조중에 첨가하므로써 효소처리를 하지않은 감귤주에 비하여 투명한 감귤주를 얻을 수 있었으며, 현탁물의 침전도 빨리 일어나 효소처리가 감귤주 제조에 유효함을 알 수 있었다. 감귤주의 숙성중에 일어나는 갈변현상을 방지 할 수 있는 연구는 차후 검토과제로 판단된다. In order to produce clear and favorable citrus wine, isolation of useful strain capable of hydrolase-producing, production of enzyme and its properties, application of enzyme to wine making were investigated. Enzyme activities of twenty-one strains of Aspergillus sp. isolated from field soils of Cheju island, and four strains preserved in our laboratory were determined. Among these, the strain CCM-4 was selected as a best strain for this purpose, and was identified as Aspergillus niger CCM-4. Optimum conditions of this strain for the production of pectic enzyme were for 3 days cultivation at 30℃ on solid culture of wheat bran or mandarin orange peel. Optimum conditions for enzyme reaction were pH 4.0 and 40℃, respectively. Enzyme treated wine was cleared much sooner and could be filtered much more rapidly than the untreated.

Differences between the Molecular Mechanisms Underlying Ruptured and Non-Ruptured Carotid Plaques, and the Significance of ABCA1

Sung Hyuk Heo,이은혜,박현희,Bum Joon Kim,Hyo Chul Youn,Young Seo Kim,Hyun Young Kim,Seong-Ho Koh,장대일 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.1

Background and Purpose Carotid plaques are a strong risk factor for ischemic stroke, and plaquerupture poses an even higher risk. Although many studies have investigated the pathogenicmechanisms of carotid plaque formation, few have studied the differences in molecular mechanisms underlying the rupture and non-rupture of carotid plaques. In addition, since early diagnosis and treatment of carotid plaque rupture are critical for the prevention of ischemic stroke, many studies have sought to identify the important target molecules involved in the rupture. However, a target molecule critical in symptomatic ruptured plaques is yet to be identified. Methods A total of 79 carotid plaques were consecutively collected, and microscopically divided into ruptured and non-ruptured groups. Quantitative polymerase chain reaction array, proteomics, and immunohistochemistry were performed to compare the differences in molecular mechanisms between ruptured and non-ruptured plaques. Enzyme-linked immunosorbent assay was used to measure the differences in ATP-binding cassette subfamily A member 1 (ABCA1) levels in the serum. Results The expression of several mRNAs and proteins, including ABCA1, was higher in ruptured plaques than non-ruptured plaques. In contrast, the expression of other proteins, including β-actin, was lower in ruptured plaques than non-ruptured plaques. The increased expression of ABCA1 was consistent across several experiments, ABCA1 was positive only in the serum of patients with symptomatic ruptured plaques. Conclusions This study introduces a plausible molecular mechanism underlying carotid plaquerupture, suggesting that ABCA1 plays a role in symptomatic rupture. Further study of ABCA1 is needed to confirm this hypothesis.

Thirteen-Week Repeated-Dose Oral Toxicity of Licorice Root in F344 Rats

Sang-Bum Koh,Jae-Hwang Jeong,Mu-Yeb Seo,Jun-Yeon Lee,Dong-Seok Seo,Hak-Soo Bark,Myung-Hwa Kang,Dae-Yong Kim,Ha-Jung Sung 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.1

The purpose of this study is to investigate a potential toxicity of licorice root (LR) in Fisher 344 rats through the 13-week repeated oral treatment. Healthy Fisher 344 rats were divided into five treatment groups (2,000, 571, 163, 47, and 13 ㎎/㎏) and one control group (vehicle) of 12 males and 12 females respectively. LR was orally administered by gavage to F344 rats once a day, 5 days per week for 13 weeks. During the test period, clinical signs, mortality, body weights, necropsy findings, hematology, serum biochemistry, organ weight, histopathology, sperm count and sperm motility were evaluated. The relative liver weights were increased significantly in males of the 2,000 and 571 ㎎/㎏ groups. However, there was no histological evidence in the liver. The increased incidence of chronic nephropathy only appeared in the 2,000 ㎎/㎏ group of both male and female rats. There were no treatment-related effects on clinical signs, mortality, body weights, necropsy findings, hematology, serum biochemistry and sperm count and motility in any treatment groups. Based on these results, no-observed adverse effect level (NOAEL) of LR was 571 ㎎/㎏ in F344 rats under the conditions of the present study.

Basilar Artery Plaque and Pontine Infarction Location and Vascular Geometry

Bum Joon Kim,Kyung Mi Lee,Hyun Young Kim,Young Seo Kim,Seong-Ho Koh,Sung Hyuk Heo,장대일 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.1

Background and Purpose Subclinical atherosclerotic plaques are common in patients with pontine infarctions (PIs) but without basilar artery (BA) stenosis. We hypothesized that BA plaque locations may differ by PI type and vertical location as well as vertebrobasilar artery geometry. Methods Ninety-six patients with PI but without BA stenosis on magnetic resonance imaging (MRI) and magnetic resonance angiography were enrolled. PIs were classified by type (paramedian, deep, or lateral) and vertical location (rostral, middle, or caudal). Patients underwent highresolution MRI to evaluate BA plaque location (anterior, posterior, or lateral). The mid-BA angle on anteroposterior view and angle between the BA and dominant vertebral artery (BA-VA angle) on lateral view were measured. Results The PIs were paramedian (72.9%), deep (17.7%), and lateral (9.4%) type with a rostral (32.3%), middle (42.7%), and caudal (25.0%) vertical location. The BA plaque locations differed by PI type (P=0.03) and vertical location (P<0.001); BA plaques were most frequent at the posterior wall in paramedian (37.1%) and caudal (58.3%) PIs and at the lateral wall in lateral (55.5%) and middle (34.1%) PIs. The BA-VA and mid-BA angles differed by BA plaque and PI vertical location; the greatest BA-VA angle was observed in patients with posterior plaques (P<0.001) and caudal PIs (P<0.001). Greatest mid-BA angles were observed with lateral BA plaques (P=0.03) and middlelocated PIs (P=0.03). Conclusions Greater mid-BA angulation may enhance lateral plaque formation, causing lateral and middle PIs, whereas greater BA-VA angulation may enhance posterior plaque formation, causing paramedian or caudal PIs.

Effect of Organic Solvents on catalyst structure of PEM fuel cell electrode fabricated via electrospray deposition

Bum-Soo Koh,Sung Chul Yi 한양대학교 세라믹연구소 2017 Journal of Ceramic Processing Research Vol.18 No.11

Proton exchange membrane fuel cells (PEMFCs) are some of the most efficient electrochemical energy sources for transportation applications because of their clean, green, and high efficiency characteristics. The optimization of catalyst layer morphology is considered a feasible approach to achieve high performance of PEMFC membrane electrode assembly (MEA). In this work, we studied the effect of the solvent on the catalyst layer of PEMFC MEAs fabricated using the electrostatic spray deposition method. The catalyst ink comprised of Pt/C, a Nafion ionomer, and a solvent. Two types of solvent were used: isopropyl alcohol (IPA) and dimethylformamide (DMF). Compared with the catalyst layer prepared using IPA-based ink, the catalyst layer prepared with DMF-based ink had a dense structure because the DMF dispersed the Pt/C-Nafion agglomerates smaller and more homogeneously. The size distribution of the agglomerates in catalyst ink was confirmed through Dynamic Light Scattering (DLS) and the microstructure of the catalyst layer was compared using field emission scanning electron microscopy (FE-SEM). In addition, the electrochemical investigation was performed to evaluate the solvent effect on the fuel cell performance. The catalyst layer prepared with DMF-based ink significantly enhanced the cell performance (1.2 A cm−2 at 0.5 V) compared with that fabricated using IPA-based ink (0.5 A cm−2 at 0.5 V) due to the better dispersion and uniform agglomeration on the catalyst layer.

Antimicobial Susceptibility Patterns of Pasteurella multocida and Streptococcus suis Isolates from Pigs during 1996~2002 in Jeonnam Province, Korea.

Sang-yoon Lee,Mi-young Kang,Jae-hyang Lim,Bong-joo Lee,Hong-bum Koh 대한수의학회 2003 대한수의학회 학술대회발표집 Vol.2003 No.-

A Case of Spondylitis and Psoas Abscess due to Salmonella

Koh, Sung Bum,Yoo, Heon,Koh, Young Cho,Kwon, Oh Kee,Park, Hyo Il,Kang, Youn Kyung 인제대학교 백병원 2001 仁濟醫學 Vol.22 No.2

Salmonella에 의한 척수의 골수염은 정상적인 면역체계나 혈색소를 가진 환자에서는 그 빈도가 매우 드물게 보고 되고 있다. 저자들은 자발성의 심한 요통으로 내원한 정상 면역을 가진 환자에서 Salmonella에 의한 척추염과 요근 농양을 경험하여 임상적, 방사선학적, 그리고 병리학적인 문헌 고찰과 함께 보고하는 바이다.

Effect of Recombinant Human FSH on Ovulation, Pregnancy and In Vitro Fertilization in Androgen-Sterilized Mice

Koh, Sang-Bum,Seo, Kwang-Suk,Kim, Seung-Chul,Ahn, Byoung-Ok,Kim, Won-Bae,Lee, Sung-Hee The Pharmaceutical Society of Korea 2002 Archives of Pharmacal Research Vol.25 No.3

The effect of a new rhFSH, PG-0801, on oocyte quality, ovulation and in vitro fertilization (IVF) was examined in androgen-sterilized mice. Experimental sterility was induced by a single subcutaneous injection of testosterone propionate (TP, 1 mg/head) into 5 day old female mice. Ovulation was generated in the 10 to 13-week old TP-injected mice by a subcutaneous rhFSH injection (1, 5 or 10 IU/head) followed 48 hours later by a second rhFSH injection (1, 5 or 10 IU/head). For comparison, a subcutaneous PMSG (5 IU/head) injection was used for folliculogenesis and a hCG (5 IU/head) injection was used for ovulation. These were administered using the same protocol. The eggs were harvested from the oviducts and counted 17 to 20 hours after the second injection. IVF was performed by adding sperms ($2{\times}10^{5}/ml{\;}to{\;}2{\times}10^{6}/ml$) to determine the functional activity of the eggs, and the fertilization rate was measured. In addition, the pregnancy rate and fetal development were examined after 15-17 days of gestation. The number of oocytes recovered from the rhFSH/rhFSH group increased dose-dependently and was slightly higher than that of the PMSG/hCG group. The pregnancy rates of the group receiving 1, 5, and 10 IU of rhFSH/rhFSH were 50%, 66.7%, and 75%, respectively, which were significantly higher than that of the control (untreated) group (0%). The numbers of viable fetuses in the 1, 5, and 10 IU/head of the rhFSH/rhFSH group ($8.0{\pm}1.50$, $8.9{\pm}1.02$, and $8.9{\pm}1.12$ fetuses/dam, respectively) were comparable to that of the 5 IU/head PMSG/hCG group ($9.4{\pm}0.94$). The mice receiving rhFSH/rhFSH and PMSG/hCG showed similar fertilization rates (around 65%) via the IVF procedure. These results demonstrate that a new rhFSH, PG-0801, may be useful for inducing ovulation in functionally infertile patients and for superovulation in ovulatory patients participating in assisted reproductive technology (ART) programs.

Poster Ⅱ : Purification and characterization of a protease isolated from earthworms ( Eisenia foetida )

Sung Hee Lee,Sang Bum Moon,Hyung Kon Koh,Hyune Hwan Lee,Jae Heung Lee 한국생화학분자생물학회 (구 한국생화학회) 1992 추계학술대회집 Vol.- No.-

Co-blowing agent에 따른 경질 폴리우레탄 폼의 물성 변화 연구

고성호(Sung Ho Koh),김상범(Sang Bum Kim) 한국가스학회 2004 한국가스학회지 Vol.8 No.2