P14.5 functions to inhibit cell migration and can be used as a prognostic marker in hepatocellular carcinoma

Song Sa,Pan Jian,Zhang Yaoyao,Xu Yuehuan,Zhang Qingmei,Xie Xiaoxun,Zhou Qingniao,Mo Farong,Luo Guorong,Chao Naixia 한국유전학회 2023 Genes & Genomics Vol.45 No.1

Background Hepatocellular carcinoma (HCC) is the most common primary liver malignancy with a high mortality rate. P14.5 is a member of the highly conserved YER057c/YIL051c/YjgF subfamily and is highly expressed in the liver. However, its low expression is associated with carcinogenesis in HCC. Objective This study aimed to investigate the role and prognostic significance of P14.5 in HCC. Methods The clinical significance of P14.5 in HCC was examined using ONCOMINE, UALCAN, Human Protein Atlas, and Kaplan–Meier plotter. The DNA methylation profile of the P14.5 promoter was examined in 103 HCC and paired precancerous tissues; the HCC cell lines HepG2, MHCC-97L, SMMC-7721, SK-Hep-1, and Huh7; and the normal hepatic cell line HL-7702 via MALDI-TOF mass spectrometry. In addition, in vitro experiments were performed to examine the effects of P14.5 overexpression on the proliferation and migration of SMMC-7721 cells. Results Low expression of P14.5 was correlated with shorter overall survival (OS) and disease-free survival (DFS) in HCC. Based on the results of MALDI-TOF mass spectrometry, no difference was observed in the methylation level between HCC cells and normal human hepatic cells and between HCC and paired precancerous tissues. Additionally, P14.5 overexpression promoted the proliferation and inhibited the migration of SMMC7721 cells in vitro. Conclusions P14.5 may serve as a prognostic biomarker in HCC and plays a role in the migration and proliferation of HCC cells. Low expression of P14.5 during hepatocarcinogenesis is not attributed to DNA methylation.

Genetic Analysis of the Liver Putative Tumor Suppressor (LPTS) Gene in Gastric Cancer

Hai Bing Zhang,Qu Zhang,Li Sa Wang,Fei Yan,Yun Lu,Zhi Qiang Wang,Jing Li,Liang Liang Zhang,Ying Yan Yu,Zheng Gang Zhu,Xiang Yin Kong,Lan Dian Hu 한국유전학회 2005 Genes & Genomics Vol.27 No.4

Hydrate Management in Deadlegs: Effect of Header Temperature on Hydrate Deposition

Zhang, Xianwei,Lee, Bo Ram,Sa, Jeong-Hoon,Kinnari, Keijo J.,Askvik, Kjell M.,Li, Xiaoyun,Sum, Amadeu K. American Chemical Society 2017 ENERGY AND FUELS Vol.31 No.11

<P>Deadlegs in oil and gas production systems often encounter hydrate plugs by deposition. Temperature is generally known to be an important variable in hydrate formation, but the effects in deadlegs are not exactly known. This study focuses on the effects of the header temperature on the hydrate deposition in gas-filled vertical deadlegs at constant wall temperature. All experiments are conducted with a methane/ethane gas mixture at constant pressure. The pipe wall temperature is kept constant while considering different header temperatures. The tests show that the header temperature has a significant impact in the hydrate deposit growth rate and distribution in the deadleg. It is also found that the hydrate deposit can, in turn, change the temperature field inside the pipe. The header temperature or the pipe temperature field can be used to estimate the hydrate distribution in the deadleg. Under the right conditions, hydrates can form a restriction in the deadleg and its location is usually close to the boundary of a hydrate-stable region. The location of the restriction can be correlated to the header temperature. At 80 °C, the location is estimated to be 15–18 ID, and at 30 °C, the location is estimated to be 9–12 ID. The results of this study contribute to the understanding of the hydrate deposition mechanism in deadlegs.</P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ef7b02095'>ACS Electronic Supporting Info</A></P>

Assessment of supervision monitoring for radiation environment around the typical research reactors in China

Sa Li,Haipeng Wang,Yanxia Zhang 한국원자력학회 2021 Nuclear Engineering and Technology Vol.53 No.12

The supervision mode, monitoring basis and monitoring scheme of radiation environment monitoringconcerning typical research reactors in China were investigated in this study. Summary and analysis wereconcluded of the present situation of supervised monitoring of radiation environment, such as monitoring objects, points, frequency and so on, based on the relevant data of monitoring points of fourtypical research reactors in China. Some experiences and existing problems were analyzed concerningthe supervised monitoring of China's research reactors. Tips on topics related to strengthen the monitoring of radiation environment around the research reactors has noted.

Manufacturing and testing of full scale prototype for ITER blanket shield block

Kim, Sa-Woong,Kim, Duck-Hoi,Jung, Hun-Chea,Lee, Sung-Ki,Kang, Sung-Chan,Zhang, Fu,Kim, Byoung-Yoon,Ahn, Hee-Jae,Lee, Hyeon-Gon,Jung, Ki-Jung Elsevier 2015 Fusion engineering and design Vol.93 No.-

<P><B>Abstract</B></P> <P>Based on the preliminary design of the ITER blanket shield block (SB) #8, the full scale prototype (FSP) has been manufactured and tested in accordance with pre-qualification program, and related R&D was performed to resolve the technical issues of fabrication. The objective of the SB pre-qualification program is to demonstrate the acceptable manufacturing quality by successfully passing the formal test program.</P> <P>316L(N)-IG stainless steel forging blocks with 1.80L×1.12W×0.43t (m) were developed by using an electric arc furnace, and as a result, the material properties were satisfied with technical specification. In the course of applying conventional fabrication techniques such as cutting, milling, drilling and welding of the forged stainless steel block for the manufacturing of the SB #8 FSP, several technical problems have been addressed. And also, the hydraulic connector of cross-forged material re-melted by electro slag or vacuum arc requires the application of advanced joining techniques such as automatic bore TIG and friction welding. Many technical issues – drilling, welding, slitting, non-destructive test and so on – have been raised during manufacturing. Associated R&D including the computational simulation and coupon testing has been done in collaboration with relevant industries in order to resolve these engineering issues.</P> <P>This paper provides technical key issues and their possible resolutions addressed during the manufacture and formal test of the SB #8 FSP, and related R&D.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 316L(N)-IG forged steel was successfully fabricated and qualified. </LI> <LI> Related R&D activities were implemented to resolve the fabrication issues. </LI> <LI> SB #8 FSP was successfully manufactured with conventional fabrication techniques. </LI> <LI> All of the validation tests were carried out and met the acceptance criteria. </LI> </UL> </P>

Partial Least Squares Based Gene Expression Analysis in EBV-Positive and EBV-Negative Posttransplant Lymphoproliferative Disorders

Wu, Sa,Zhang, Xin,Li, Zhi-Ming,Shi, Yan-Xia,Huang, Jia-Jia,Xia, Yi,Yang, Hang,Jiang, Wen-Qi Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Post-transplant lymphoproliferative disorder (PTLD) is a common complication of therapeutic immunosuppression after organ transplantation. Gene expression profile facilitates the identification of biological difference between Epstein-Barr virus (EBV) positive and negative PTLDs. Previous studies mainly implemented variance/regression analysis without considering unaccounted array specific factors. The aim of this study is to investigate the gene expression difference between EBV positive and negative PTLDs through partial least squares (PLS) based analysis. With a microarray data set from the Gene Expression Omnibus database, we performed PLS based analysis. We acquired 1188 differentially expressed genes. Pathway and Gene Ontology enrichment analysis identified significantly over-representation of dysregulated genes in immune response and cancer related biological processes. Network analysis identified three hub genes with degrees higher than 15, including CREBBP, ATXN1, and PML. Proteins encoded by CREBBP and PML have been reported to be interact with EBV before. Our findings shed light on expression distinction of EBV positive and negative PTLDs with the hope to offer theoretical support for future therapeutic study.

Characterization of Gas Hydrate Deposition in Deadlegs for the Risk Management of Oil & Gas Production Systems

Jeong-Hoon Sa,Xiznwei Zhang,Amadeu Sum 한국신재생에너지학회 2020 한국신재생에너지학회 학술대회논문집 Vol.2020 No.8

Establishment of Paclitaxel-resistant Breast Cancer Cell Line and Nude Mice Models, and Underlying Multidrug Resistance Mechanisms in Vitro and in Vivo

Chen, Si-Ying,Hu, Sa-Sa,Dong, Qian,Cai, Jiang-Xia,Zhang, Wei-Peng,Sun, Jin-Yao,Wang, Tao-Tao,Xie, Jiao,He, Hai-Rong,Xing, Jian-Feng,Lu, Jun,Dong, Ya-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: Breast cancer is a common malignant tumor which affects health of women and multidrug resistance (MDR) is one of the main factors leading to failure of chemotherapy. This study was conducted to establish paclitaxel-resistant breast cancer cell line and nude mice models to explore underlying mechanisms of MDR. Methods: The breast cancer drug-sensitive cell line MCF-7 (MCF-7/S) was exposed in stepwise escalating paclitaxel (TAX) to induce a resistant cell line MCF-7/TAX. Cell sensitivity to drugs and growth curves were measured by MTT assay. Changes of cell morphology and ultrastructure were examined by optical and electron microscopy. The cell cycle distribution was determined by flow cytometry. Furthermore, expression of proteins related to breast cancer occurrence and MDR was tested by immunocytochemistry. In Vivo, nude mice were injected with MCF-7/S and MCF-7/TAX cells and weights and tumor sizes were observed after paclitaxel treatment. In addition, proteins involved breast cancer and MDR were detected by immunohistochemistry. Results: Compared to MCF-7/S, MCF-7/TAX cells had a higher resistance to paclitaxel, cross-resistance and prolonged doubling time. Moreover, MCF-7/TAX showed obvious alterations of ultrastructure. Estrogen receptor (ER) expression was low in drug resistant cells and tumors while expression of human epidermal growth factor receptor 2 (HER2) and Ki-67 was up-regulated. P-glycoprotein (P-gp), lung resistance-related protein (LRP) and glutathione-S-transferase-${\pi}$ (GST-${\pi}$) involved in the MDR phenotype of resistant cells and tumors were all overexpressed. Conclusion: The underlying MDR mechanism of breast cancer may involve increased expression of P-gp, LRP and GST-${\pi}$.

miR-1301/TRIAP1 Axis Participates in Epirubicin-Mediated Anti-Proliferation and Pro-Apoptosis in Osteosarcoma

Lijun Yu,Min Meng,Yun Bao,Chao Zhang,Bei Gao,Rina Sa,Wenyuan Luo 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.9

Purpose: Epirubicin is one of the most effective drugs against osteosarcoma. miR-1301 is involved in the occurrence and developmentof osteosarcoma. Whether miR-1301 is responsible for the chemosensitivity of osteosarcoma cells to epirubicin remainslargely unknown. Materials and Methods: U2OS and SAOS-2 cells were treated with various concentrations of epirubicin. Flow cytometry was employedto evaluate cell apoptotic rate. Cell proliferation was measured by Cell Counting Kit-8 assay. Western blot and quantitativereal-time polymerase chain reaction were utilized to detect the expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 assaciated X protein(Bax), cleaved-caspase-3, cleaved-poly (ADP-ribose) polymerases (PARP1), TP53-regulated inhibitor of apoptosis 1 (TRIAP1),and microRNA-1301 (miR-1301). The relationship between miR-1301 and TRIAP1 was determined by luciferase reporter assay. Results: Epirubicin inhibited proliferation in a dose-dependent manner, induced apoptosis, decreased the expression of Bcl-2,and increased the expressions of Bax, cleaved-caspase-3, and cleaved-PARP1 in osteosarcoma cells. miR-1301 was downregulatedin U2OS and SAOS-2 cells. Importantly, epirubicin significantly increased the levels of miR-1301. Overexpression of miR-1301suppressed proliferation and promoted apoptosis. Interestingly, those effects were enhanced by epirubicin. In contrast, miR-1301depletion attenuated the epirubicin-mediated anti-osteosarcoma effect. miR-1301 negatively regulated the expression of TRIAP1in U2OS and SAOS-2 cells. Furthermore, epirubicin inhibited the mRNA and protein levels of TRIAP1 by upregulating miR-1301levels. Epirubicin suppressed cell proliferation by downregulating TRIAP1. Conclusion: miR-1301 was implicated in the chemosensitivity of osteosarcoma to epirubicin by modulating TRIAP1.

Study on the Hydrothermal Synthesis and Optical Properties of YVO4:Dy3+ Phosphor Powders

Hong-Tao Liu,Yan Liang,Xiao-Yong Gao,Sa Zhang,Xian-Wei Zhao,Xian-Mei Chen 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.8

YVO4:Dy3+ phosphor powders were synthesized by using the hydrothermal method under differentpH conditions. The crystallization, surface morphology, lattice vibration, optical properties,luminescent mechanism and influencing factors of the obtained YVO4:Dy3+ phosphor powders werecarefully studied and analyzed in detail. All of the YVO4:Dy3+ phosphor powders had tetragonalstructures, and the pH value had a significant impact on the surface morphology, structure andoptical properties of the synthesized samples. Strong acidic and alkali environments were favorablefor the crystallization of YVO4:Dy3+phosphor powders, and the YVO4:Dy3+ phosphor powderssynthesized under strong alkali environments had the best luminescent properties.