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CUI, Xiang-Shun,LI, Xing-Yu,YIN, Xi-Jun,KONG, IL Keun,KANG, Jason-Jongho,KIM, Nam-Hyung 家畜繁殖硏究所 2007 Journal of Reproduction and Development Vol.53 No.2
<P>Maternal gene expression is an important biological process in oocyte maturation and early cleavage. To gain insights into oocyte maturation and early embryo development, we used microarray analysis to compare the gene expression profiles of germinal vesicle (GV)- and metaphase II (MII)-stage oocytes. The differences in spot intensities were normalized and grouped using the Avadis Prophetic software platform. Of the 12164 genes examined, we found 1682 genes with more highly expression in GV-stage oocytes than in MII-stage oocytes, while 1936 genes were more highly expressed in MII-stage oocytes (P<0.05). The genes were grouped on the basis of the Panther classification system according to their involvement in particular biological processes. The genes that were up-regulated in GV oocytes were more likely to be involved in protein metabolism and modification, the mitotic cell cycle, electron transport, or fertilization or belong to the microtubule/cytoskeletal protein family. The genes specifically upregulated in the MII oocytes were more likely to be involved in DNA replication, amino acid metabolism, or expression of G protein-coupled receptors and signaling molecules. Identification of genes that are preferentially expressed at particular oocyte maturation stages provides insights into the complex gene regulatory networks that drive oocyte maturation and fertilization.</P>
Chromatin, microtubule and microfilament configurations in the canine oocyte
Jin, Yong-Xun,Lee, Hyo-Sang,Yin, Xi-Jun,Cui, Xiang-Shun,Kong, Il-Keun,Kim, Nam-Hyung CSIRO Publishing 2006 Reproduction, fertility, and development Vol.18 No.8
<P>In the present study, we observed chromatin, microtubule and microfilament distribution in canine oocytes. The germinal vesicle (GV) chromatin of canine oocytes was classified into four configurations (GV-I, -II, -III and -IV) based on the degree of chromatin separation and condensation. Oocytes recovered from follicular phase ovaries had a greater amount (68%, P < 0.05) of GV-III or GV-IV chromatin than did those from non-follicular phase ovaries (35%). The majority (86.7%) of in vivo ovulated oocytes were at GV-IV. The rates of development to GV breakdown/metaphase I/metaphase II were higher in oocytes recovered from follicular ovaries than from non-follicular ovaries. Immunostaining results revealed cytoplasmic microtubules present in all GV-stage oocytes. Following GV breakdown, microtubular asters were produced from condensed chromatin. The asters appeared to be elongated, and encompassed condensed chromatin particles to form meiotic metaphase chromatin. Microfilaments were located in the cortex and around the GV. During meiotic maturation, a microfilament-rich area, in which the chromatin is allocated, was observed in the oocyte. Our results indicate that oocytes recovered from follicular ovaries were in an advanced stage of GV, and were more competent to complete maturation compared to those from non-follicular phase ovaries. Both microtubules and microfilaments are closely associated with reconstruction of chromatin during meiotic maturation in canine oocytes.</P>
Jing Zhang,Xiao Kun Teng,Li Zhen Si,Ping Tong Zhou,Xiang Yin Kong,Lan Dian Hu 한국유전학회 2008 Genes & Genomics Vol.30 No.4
Hair follicle (HF) morphogenesis depends upon a delicate balance between cell proliferation and apoptosis, both of which are known to be controlled by a number of signal pathways. In this study, we utilize the uncv homozygous mouse to investigate the underlying mechanisms giving rise to the relevant phenotypes. In so doing, we have detected changes in the EGFR, Akt and MAPK signaling pathways. Our results show that the expression patterns of active Akt, MAPK and EGFR are altered with regard to localization in the outer root sheath (ORS) of the wild type mouse and in the follicular bulbs of the uncv homozygous mouse. These data suggest that EGFR signaling may play different roles in different cellular locations. Further, ectopic EGFR signaling in the follicular bulbs of the uncv homozygous mouse may lead to catagen (entrance into the apoptosis phase). Moreover, when the EGFR ligand was overexpressed in the uncv heterozygous mouse, aggravation of HF disordered development ensued. Together, these results demonstrate that EGFR participates in a functionally compartmentalized signaling network that controls follicular development homeostasis.