梔子大黃湯과 茵蔯梔子湯 Extract가 白鼠의 肝損傷에 미치는 影響

車宗泰,柳道坤,康舜洙 圓光大學校 韓醫學硏究所 1987 圓光醫科學 Vol.3 No.1

An attempt was made to evaluate the effects of Chijadaewhangtang extract (CDE) and Inchinchijatang extract (ICE) on the recovery of hepatotoxicity induced acute treatment of carbon tetrachloride and results were as follows: 1. When rats of carbon tetrachloride-intoxication were treated with CDE and ICE, the serum aspartate aminotransferase activities released from liver were decreased. In ICE treated group, there were significant decrement after 12 hours and more fast return to normal values in comparison with CDE treated group. 2. In CDE and ICE-treated group, as compared with only carbon tetrachloride-treated group there were inhibition of serum alanine aminotransferase elevation and significant decrement was observed in ICE treated group after 48 hours and CDE treated group after 72 hours. 3. Serum alkaline phosphatase values were significantly diminished in CDE and ICE-treated group compared with only carbon tetrachloride intoxicated group. Meaningful diminution was observed in ICE administered group after 48 hours and CDE administered group after 96 hours. 4. Significant decrement of serum lactate dehydrogenase elevation after 96 hours was observed in CDE and ICE-treated group and more fast recover to normal values. 5. Administration of CDE and ICE shortened prothrombin time of plasma and significant recovery was showed in ICE treated group after 72 hours and CDE treated group after 96 hours. From these results, the recuperative effects of CDE and ICE on carbon tetrachloride-induced hepatotoxicity could be verified experimentally.

滋賢丸 煎湯液이 無麻醉 家兎의 腎臟機能에 미치는 影響

鄭銀卿,金庚植,柳道坤,李昊燮 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

This study was carried out to evaluate the effects of Jasinhwan(滋腎丸) water extract on the renal function and arterial blood pressure. The results of this study were as follows : 1. Urine volume, renal plasma flow, and glomerular filtration rate were significant increased after intravenous administration of Jasinhwan water extract(0.2㎖/㎏). 2. Urine volume, renal plasma flow, glomerular filtration rate, and urinary excretion of electrolytes were significant increased after intravenous administration of Jasinhwan water extract(0.4㎖/㎏). 3. Heart rate was significant decreased after intravenous administration of Jasinhwan water extract(0.4㎖/㎏). These results suggest that the increased of ruine volume after intravenous administration of Jasinhwan water extract was related with the changed of renal hemodynamics and the increased of urinary excretion of electrolytes.

淸肺散 煎湯液이 家兎의 腎臟機能에 미치는 影響

張根國,李昊燮,柳道坤 圓光大學校 韓醫學硏究所 1992 원광한의학 Vol.2 No.1

To evalute effect of Chungpyesan(淸肺散) Water Extract on the renal function, blood pressure and heart rate, experiment performed in rabbits. The results obtained were as follows : 1. Intravenous administration of Chungpyesan Water extract at the doses of 0.75㎖/㎏ significantly increased renal plasma flow, glomerular filtration rate, urinary excretion of electrolytes and urinary excretion of free water clearance, in particular, urine volume. 2. Urine volume, glomerular filtration rate and urinary excretion of electrolytes were increased remarkably after intravenous administration of Chungpyesan Water extract at the doses of 1.5㎖/㎏. 3. Intravenous administration of Chungpyesan Water extract at the doses of 0.75㎖/㎏ significantly increased heart rate. 4. Intravenous administration of Chungpyesan Water extract at the doses of 1.5㎖/㎏ significantly decreased blood pressure, whereas heart rate remakably increased. These results suggest that Chungpyesan Water extract has a diuretic effects, which may be caused by urinary excretion of electrolytes, renal hemodynamics.

폐암세포에 대한 附子 추출물의 독성 효과

권강범,김은경,문형철,송용선,류도곤 한국전통의학연구소 2006 한국전통의학지 Vol.15 No.1

The aim of this study was to investigate the cytotoxic effect and its mechanism on Radix Aconiti(RA) extract in lung cancer cell lines. RA extract treatment decreased the cell viability in a dose-dependent fashions in lung cancer cells including A549, H460, H23 and H157 cells. Many investigators reported that A549 and H460 cells expressed wild-type p53, but H23 and H157 cells preserved mutated p53. After treatment with RA extract in A549 and H460 cells, we measured the expression of p53 protein levels using Western blot, analysis. In both cells treated with RA extracts, p53 protein expressions were increased in a dose-dependent manner. In our experiments, RA extracts also have cytotoxic effects in H23 and H157, which have mutated p53. Treatment with RA extract decreased bcl-2 protein expressions in both cells. These results suggest that RA extracts have cytotoxic effects via p53 expression increase and bcl-2 inhibitable pathways in A549, H460 cells and H23, H157 cells, respectively.

GO에 의해 유도된 培養 脊髓感覺神經細胞 독성에 대한 鎭肝熄風湯의 방어효과

박광수,이호승,김인수,강길성,김인규,김인섭,류도곤 한국전통의학연구소 2003 한국전통의학지 Vol.13 No.1

To evaluate the effect of Jingansikpungtang water extract on cultured mouse spinal sensory neuron which was inhibited by glucose oxidase(GO)-induced cytotoxicity, NR assay and TBARS assay for lipid peroxidation were carried out after the cultured mouse spinal sensory neuron were pre-incubated with various concentrations of Jingansikpungtang water extract for 3 hours prior to exposure of GO. GO, a oxygen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay. Jingansikpungtang water extract have efficacy of decreasing lipid peroxidation increasing by GO in cultured mouse spinal sensory neuron. From above the results, it is concluded that Jingansikpungtang has marked efficacy as a treatment for the damages caused in the GO-mediated oxidative process.

丹參飮 煎湯液이 心筋細胞 搏動數에 미치는 影響

손창식,권강범,김상범,이호승,서은아,이호섭,류도곤 대한동의병리학회 2001 동의생리병리학회지 Vol.15 No.2

In order to elucidate toxic mechanism of myocardial damage and protective effect of herbal extract, Dansamyeum (丹參飮) against cytotoxic effect of xanthine oxidase/hypoxanthine(XO/HX) and cardioprotective effect of Dansamyeum (丹參飮) were examined by MTT assay and heart beating rate after cultured myocardial cells derived from neonatal mouse were treated with various concentrations of XO/HX, a free radical. The results of these experiments were fellows : XO/HX, a oxygen radical, decreased the survial rate on MTT assay in cultured myocardial cells. Dansmyeum(丹參欲) water extract have efficacy of increasing heart beating rate decreased by XO/HX in cultured myocardial cells. Radix Salviae Miltiorrhizae and Lignum Santali Album water extract have efficacy of increasing heart beating rate decreased by XO/HX in cultured myocardial cells. From above the results, it is suggested that XO/HX showed toxic effect in cultured myocardial cell derived from neonatal mouse and herbal extract such as Dansamyeum(丹參飮) is very effective in the prevetion of XO/HX cardiotoxicity. Key ward : Dansamyeum(丹參飮), Xanthine Oxidase/Hypoxanthine(XO/HX), myocardial Cell, Radix Salviae Miltiorrhizae, Lignum Santali Album, MTT, beating rate

津液의 調節過程에 對한 硏究

柳道坤(Ryu Do Gon),鄭遇悅(Jeong Woo Yeal) 대한한의학회 1989 대한한의학회지 Vol.10 No.1

숙지황, 부자 전탕액이 고혈압 백서의 혈압에 미치는 영향

류도곤 ( Do Gon Ryu ),윤용갑 ( Yong Gab Yun ),노진구 ( Jin Goo No ),이호섭 ( Ho Sub Lee ) 대한경락경혈학회 2001 Korean Journal of Acupuncture Vol.18 No.1

한의학에 있어서 음양의 조절은 매우 중요하다. 숙지황은 음허를 치료하는 주약이며, 부자는 양허를 치료하는 주약이다. 한의학에서 신성고혈압의 발생은 음허와 관련이 있다고 하였다. 본 연구는 실험적 신성고혈압과 자연발증고혈압 백서에 숙지황과 부자 전탕액을 투여 후 혈압의 변동과 혈장 renin 활성도, aldosterone 및 심방이뇨호르몬 (atrial natriuretic hormone)의 변동을 관찰하였다. 숙지황 투여후 실험적 신성고혈압 백서의 혈압은 감소하였다. 부자 전탕액 투여 후 자연발증고혈압 백서의 혈압 상승이 억제되었다. 숙지황 및 부자 전탕액 투여 후 자연발증고혈압 백서의 혈장 renin 활성도는 감소하였다. 부자 전탕액 투여 후 신성고혈압 백서의 혈장 ANP 농도는 감소하였다. 부자 전탕액의 혈압강하 작용은 ANP 및 aldosterone 농도의 변동과 관련이 있다고 사료된다.

SNAP 유도성 RINm5F 세포 독성에 대한 黃連 추출물의 방어효과

류도곤(Do-Gon Ryu),권강범(Kang-Beom Kwon),양정예(Jeong-Yeh Yang),김은경(Eun-Kyung Kim),김강산(Kang-San Kim) 대한한의학회 2003 대한한의학회지 Vol.24 No.2

脊髓 感覺神經節 細胞에 대한 重金屬 및 酸素遊離氣의 細胞損傷에 미치는 韓藥劑의 神經細胞損傷 回復에 미치는 影響에 對한 硏究

柳道坤,李廷憲,成疆慶,李建穆,辛民敎,鄭遇悅,李星根,洪起年,尹向錫,李康昌,宋昊俊,禹元洪,朴承澤,田炳薰 대한동의병리학회 2000 동의생리병리학회지 Vol.14 No.1

중금속의 독성을 산화적 손상 측면에서 규명하며 동시에 중금속의 산화적 손상에 의한 한약추출물의 효과를 항산화적측면에서 조사하기 위하여 생쥐의 척수 감각신경절세포에 여러 농도의 FeSO₄를 처리한 후 이의 독성효과를 조사하였다. FeSO₄는 생쥐의 배양 척수 감각신경질세포에 처리한 농도와 시간에 비례하여 세포생존율을 유의하게 감소시켰으며, 15μM FeSO₄의 농도에서 척수 감각신경절세포를 4시간 동안 배양한 결과 MTT50 과 NR50 값을 나타냈으며 이는 고독성인 것으로 나타났다. [고독성; 100μM> MTT50, NR50]. 한편, 한약추출물인 원두충(EU)과 음양곽(EK) 및 감초(GC)는 FeSO₄에 의해서 유도된 신경독성에 대하여 세포생존율을 현저히 증가시킴으로서 유의한 방어효과를 보였다. 이상에서 FeSO₄는 생쥐의 배양 척수 감각신경절세포에 고독성으로 나타났으며, 또한 음양곽이나 익지인 및 감초와 같은 선택적인 한약추출물이 FeSO₄에 의해서 유도된 신경독성을 효과적으로 방어하였다. To clarify the mechanism between neurotoxity of metal and oxidative stress, toxic effect of FeSO₄was examined by MTT assay and NR assay in cultured spinal dorsal root ganglion(DRG) neurons from mouse. And also neuroprotective effects of antioxidant and several herb extracts on FeSO₄decreased cell viability in dose and time dependentry in cultured mouse spinal DRG neurons. MTT50 and NR50 values were determined at 15μM FeSO₄for 4 hours in these cultures, and this value was heighly toxic; MTT50, NR50 < 100μM). Glutathione markedly increasd cell viability after preincubation of DRG neros for 2 hours in the media containing cocentrations of 2-6mM glutathione. In neuroprotective of herb extracts, Eucommia lmoides Oliver(EU), Epimedium koreanum Nakai(EK) and Glycyrrhiza uralensis Fisch(GC) were very effective in blocking the neurotoxicity induced by FeSO₄in cultured mouse spinal DRG neurons. These results suggested that FeSO₄was heighly toxic in cultured mouse spinal DRG neurons, and selective herb extracts such as Eucommia ulmoides Oliver(EU), Epimedium koreanum Nakai(EK) and Glycyrrhiza uralensis Fisch(GC) were effective in blocking the FeSO₄-induced neurotoxicity in cultured mouse spinal DRG neurons.