Peroxiredoxin V Regulates Aco2, Acadm, and Acox1 Activity in Hypoxic Kidney

Tae‐Hoon Lee,Hee‐Young Yang,Joseph Kwon,Hoon‐In Choi,Lina Ren,Ung Yang,Byung‐Ju Park,Zae Young Ryoo 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and nucleus. Prdx V has been reported to protect a wide range of cellular environments as antioxidant enzyme, and its dysfunctions may be implicated in several diseases, such as cancer, inflammation, and neurodegenerative disease. Identification and relative quantification of proteins affected by Prdx V may help identify novel signaling mechanisms that are important for oxidative stress response. However, the role of Prdx V in the modulation of hypoxia‐related cellular response is not studied yet. In order to examine the function of endogenous Prdx V in hypoxic condition in vivo, we generated a transgenic mouse model with Prdx V siRNA expression controlled by U6 promoter. Of many tissues, the knockdown of Prdx V expression was displayed in kidney, lung, and liver, but not spleen and skin. We conducted on the basis of nano‐UPLC‐MSE proteomic study to identify the Prdx V‐affected protein networks in hypoxic kidneys. In this study, we identified protein networks associated with oxidative stress, fatty acid metabolism, and mitochondrial dysfunction. Our results indicated that Prdx V affected to regulation of kidney homeostasis under hypoxia stress.

광대역 통신망에서의 트래픽 제어기술에 관한 연구

안기중,유인태 濟州大學校工科大學産業技術硏究所 1995 尖端技術硏究所論文集 Vol.6 No.-

In this paper, an ATM cell monitor/control algorithm is proposed to enhance the efficiency of bandwidth management and statistical multiplexing for various types of cell traffics. This algorithm is based on the usage parameter control and bandwidth allocations. The traffic is monitored and controlled by counters, lookup table and cell control unit to reduce the burden of the traffic overload. And for the bandwidth management the entire bandwidth is separated into two classes : the basic bandwidth is allocated to each type of traffic and the shared bandwidth may be used when more bandwidth is required due to the burst. The network resources can be allocated to more virtual connections accordingly.

광대역 통신망에서의 트래픽 제어기술에 관한 연구

유인태,안기중 濟州大學校 産業技術硏究所 1995 산업기술연구소논문집 Vol.6 No.-

In this paper, an ATM cell monitor/control algorithm is proposed to enhance the efficiency of bandwidth management and statistical multiplexing for various types of cell traffics. This algorithm is based on the usage parameter control and bandwidth allocations. The traffic is monitored and controlled by counters, lookup table and cell control unit to reduce the burden of the traffic overload. And for the bandwidth management the entire bandwidth is seperated into two classes : the basic bandwidth is allocated to each type of traffic and the shared bandwidth may be used when more bandwidth is required due to the burst. The network resources can be allocated to more virtual connections accordingly.

Comparative Evaluation of Nanofibrous Scaffolding for Bone Regeneration in Critical-Size Calvarial Defects

Woo, Kyung Mi,Chen, Victor J.,Jung, Hong-Moon,Kim, Tae-Il,Shin, Hong-In,Baek, Jeong-Hwa,Ryoo, Hyun-Mo,Ma, Peter X. Mary Ann Liebert 2009 Tissue engineering. Part A Vol.15 No.8

<P>In a previous study we found that nanofibrous poly(l-lactic acid) (PLLA) scaffolds mimicking collagen fibers in size were superior to solid-walled scaffolds in promoting osteoblast differentiation and bone formation in vitro. In this study we used an in vivo model to confirm the biological properties of nanofibrous PLLA scaffolds and to evaluate how effectively they support bone regeneration against solid-walled scaffolds. The scaffolds were implanted in critical-size defects made on rat calvarial bones. Compared with solid-walled scaffolds, nanofibrous scaffolds supported substantially more new bone tissue formation, which was confirmed by micro-computed tomography measurement and von Kossa staining. Goldner's trichrome staining showed abundant collagen deposition in nanofibrous scaffolds but not in the control solid-walled scaffolds. The cells in these scaffolds were immuno-stained strongly for Runx2 and bone sialoprotein (BSP). In contrast, solid-walled scaffolds implanted in the defects were stained weakly with trichrome, Runx2, and BSP. These in vivo results demonstrate that nanofibrous architecture enhances osteoblast differentiation and bone formation.</P>

몽골 촉트체치 지역 동 산출지 예비조사결과

김인준(In Joon Kim),이재호(Jae Ho Lee),류충렬(Chung-Ryul Ryoo),이범한(Bum-Han Lee),진광민(Kwang Min Jin),오트곤-에르덴 다바수(Otgon-Erdene Davaasuren),허철호(Chul-Ho Heo),남형태(Hyeong Tae Nam ) 대한자원환경지질학회 2017 자원환경지질 Vol.50 No.4

촉트체치 지역은 남고비에 위치하며 석탄기 후기에서 페름기 초기에 관입한 흑운모 화강암 중 일부가 반암이 관입 하면서 알카리 화강암화 된 부분과 관련된 동 광상이다. 말라카이트가 집중적으로 산출되는 지역은 녹니석, 녹렴석으로 변질된 프로필리틱 변질대가 광범위하게 나타난다. 격자지구화학탐사와 연장부 조사 결과 말라카이트가 산출되는 곳은 30개 지점으로 휴대용 XRF로 측정한 동함량은 1.080~18.300%로 나타났으며, 화학분석 결과 동함량은 13개 지점에서 각각 1.080%~32.900로 높게 나타났다. X-선 회전 분석결과와 현미경 관찰결과 동광물은 말라카이트가 주이며, 남동석, 퍼민제타이트, 황철석과 황동석 등이 가끔 산출되며, 그 외에 은 광물인 능홍은석이 산출된다. 또한 열수변질 광물인 딕카이트가 산출되며, 변질산물인 방해석, 녹니석, 녹염석 등도 산출된다. 광화작용은 반암관입 후에 남은 잔류마그마에서 열수용액이 올라오면서 반암 주변 일부 화강암과 정단층 상반부에만 선택적으로 일어난 것으로 사료된다 Tsogttsetsii area, an intrusive complex associated with Cu mineralization, is located in the South Gobi, Mongolia. We performed the cross geochemical and extended exploration survey in Tsogttsetsii area. Cu mineralization in Tsogttsetsii area is porphyry Cu type related with alkali granite intruded in Late Carboniferous to Early Permian. In the concentrated occurring to malachite appears extensively prophylitic alteration zone having a chlorite and epidote. As results of the survey, Cu contents of potable XRF and of chemical composition for altered rocks ranges 1.08 to 18.3% in the 30 points and 1.08 to 32.9% in the 13 points, respectively. Ore minerals identified in XRD analysis and polarizing microscope that samples of copper oxides were composed mainly of malachite, azurite, permingeatite and cuprite and the other minerals are pyrite, chalcopyrite, pyrargyrite, dickite, calcite, chlorite and epidote. Mineralization can be considered occurring to selectively some granite of the surrounding aplite and faults in the only upper part coming up the hydrothermal solution of the remaining residual magma after the aplite intrusion.

에나멜 상피종성 및 유두상 두개인두종의 임상상과 자기공명영상 ( MRI ) 소견의 비교

강태욱,양태영,김광원,이문규,민용기,이명식,정재훈,김종현,나동규,정인경,함종렬,권성욱,조건영,이지민,배문희,서연림,유재욱 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.2

Background : Craniopharyngioma is a suprasellar or intrasellar epithelial neoplasm that occurs in both children and adults. It accounts for 1.2 to 3 % of intracranial tumors with an incidence of 0.5 to 2 cases per one million populations each year. Recently, it has been postulated that it may have two pathogenetically separate subtypes, which are adamantinous and papillary craniopharyngioma, and that their clinical features may be different. However, there are some disagreements in this postulation. Therefore, we studied 22 consecutive patients with craniopharyngioma to evaluate the differences in clinical features and MRI findings between two subtypes. Methods : We studied 22 patients with histologically proven craniopharyngioma after surgery at Samsung Medical center from 1995 to 1999. Thirteen patients were male, and nine patients were female. The average age was 30 years, with a range from 1 to 58 years. We divided 22 patients into two histopathologically separate subtypes; adamantinous and papillary subtypes. We compared the clinical features and MRI findings of two subtypes by reviewing medical records. Results : Out of 22 patients with craniopharyngioma, 19 patients had an adamantinous subtype and 3 patients had a papillary subtype. The adamantinous subtype occurred frequently in the fifth decade and below twenty years, while the papillary subtype occurred predominantly in forth and fifth decades. The adamantinous subtype located in suprasellar or intrasellar portion as well as extrasellar portion, while the papillary subtype was restricted to the suprasellar location. The average tumor size of the adamantinous subtype was 3.7 cm, with a range from 1.4 to 6.0 cm, which was larger than that of the papillary subtype (average size 1.8 cm with a range from 1.5 to 2.3 cm, p $lt; 0.05). The adamantinous subtype was predominantly cystic, while the papillary subtype was predominantly solid (p $lt; 0.05). There were no significant differences in the preoperative clinical features and the postoperative complications between two subtypes. Conclusion : The adamantinous subtype had two peaks of occurrence in the fifth decade and below twenty years, while the papillary subtype occurred predominantly in forth and fifth decades. The adamantinous subtype was larger and had cystic portion, while the papillary subtype was smaller and had solid portion. The preoperative clinical features and the postoperative complications between two subtypes seemed not to be different (J Kor Soc Endocrinol 15:170-178, 2000).

Four novel RUNX2 mutations including a splice donor site result in the cleidocranial dysplasia phenotype

Kim, Hyo-Jin,Nam, Soon-Hyeun,Kim, Hyun-Jung,Park, Hyo-Sang,Ryoo, Hyun-Mo,Kim, Shin-Yoon,Cho, Tae-Joon,Kim, Seung-Gon,Bae, Suk-Chul,Kim, In-San,Stein, Janet L.,van Wijnen, Andre J.,Stein, Gary S.,Lian, Liss 2006 Journal of Cellular Physiology Vol.207 No.1

<P>Cleidocranial dysplasia (CCD) is an autosomal dominant disorder caused by haploinsufficiency of the RUNX2 gene. In this study, we analyzed by direct sequencing RUNX2 mutations from eleven CCD patients. Four of seven mutations were novel: two nonsense mutations resulted in a translational stop at codon 50 (Q50X) and 112 (E112X); a missense mutation converted arginine to glycine at codon 131 (R131G); and an exon 1 splice donor site mutation (donor splice site GT/AT, IVS1 + 1G > A) at exon 1–intron junction resulted in the deletion of QA stretch contained in exon 1 of RUNX2. We focused on the functional analysis of the IVS1 + 1G > A mutation. A full-length cDNA of this mutation was cloned (RUNX2Δe1) and expressed in Chinese hamster ovary (CHO) and HeLa cells. Functional analysis of RUNX2Δe1 was performed with respect to protein stability, nuclear localization, DNA binding, and transactivation activity of a downstream RUNX2 target gene. Protein stability of RUNX2Δe1 is similar to wild-type RUNX2 as determined by Western blot analysis. Subcellular localization of RUNX2Δe1, assessed by in situ immunofluorescent staining, was observed with partial retention in both the nucleus and cytoplasm. This finding is in contrast to RUNX2 wild-type, which is detected exclusively in the nucleus. DNA binding activity was also compromised by the RUNX2Δe1 in gel shift assay. Finally, RUNX2Δe1 blocked transactivation of the osteocalcin gene determined by transient transfection assay. Our findings demonstrate for the first time that the CCD phenotype can be caused by a splice site mutation, which results in the deletion of N-terminus amino acids containing the QA stretch in RUNX2 that contains a previously unidentified second nuclear localization signal (NLS). We postulate that the QA sequence unique to RUNX2 contributes to a competent structure of RUNX2 that is required for nuclear localization, DNA binding, and transactivation function. J. Cell. Physiol. 207: 114–122, 2006. © 2005 Wiley-Liss, Inc.</P>

무선 ATM 망에서 페이징 트래픽 부하의 감소를 위한 동적 페이징 영역 관리 기법

이태로(Tae Ro Lee),이성원(Sung Won Lee),한치근(Chi Geun Han),유인태(In Tae Ryoo) 한국정보처리학회 2000 정보처리학회논문지 Vol.7 No.8

In this paper, we consider ATM-Forum''s WATM(Wireless Asynchronous Transfer Mode) based third generation wireless multimedia network environments, where many simplified AP(Access Point)s are managed by one M.E.SW(Mobility Enabled ATM Switch). Also, we propose an efficient paging mechanism for WATM network environments using variable grouping size and dynamic group paging sequence method. Then, we evaluate and analyze the performance of the proposed and conventional methods. That is, most of conventional algorithms such as blanket-polling, sequential-polling, and fixed size grouping method are compared with the proposed variable and dynamic grouping method. Analysis results show that simple blanket-polling paging method shows slightly better performance at low overload environments. However, as the traffic density increases, the proposed method shows better performance than the conventional methods. Main reason of the performance improvements is due to the variable grouping size and MS(Mobile Station) based dynamic paging sequence mechanism. Moreover, the proposed method requires very simple history information about MS, and only last AP information is required. Thus, it is possible to implement efficiently in real world.

Iterative V-BLAST Decoding Algorithm in the AMC System with a STD Scheme

Lee, Keun-Hong,Ryoo, Sang-Jin,Kim, Seo-Gyun,Hwang, In-Tae The Korea Institute of Information and Commucation 2008 Journal of information and communication convergen Vol.6 No.1

In this paper, we propose and analyze the AMC (Adaptive Modulation and Coding) system with efficient turbo coded V-BLAST (Vertical-Bell-lab Layered Space-Time) technique. The proposed algorithm adopts extrinsic information from a MAP (Maximum A Posteriori) decoder with iterative decoding as a priori probability in two decoding procedures of V-BLAST scheme; the ordering and the slicing. Also, we consider the AMC system using the conventional turbo coded V-BLAST technique that simply combines the V-BLAST scheme with the turbo coding scheme. And we compare the proposed decoding algorithm to a conventional V-BLAST decoding algorithm and a ML (Maximum Likelihood) decoding algorithm. In addition, we apply a STD (Selection Transmit Diversity) scheme to the systems for better performance improvement. Results indicate that the proposed systems achieve better throughput performance than the conventional systems over the entire SNR range. In terms of transmission rate performance, the suggested system is close in proximity to the conventional system using the ML decoding algorithm.

An Efficient Power Control Algorithm for Satellite Communications Systems with ATC

Kim, Byung-Gi,Ryoo, Sang-Jin,Hwang, In-Tae The Korea Institute of Information and Commucation 2008 Journal of information and communication convergen Vol.6 No.2

In this paper, modified power control algorithms are proposed for a satellite mobile communications system with ATC (ancillary terrestrial component). In order to increase system capacity and reduce the transmitting power of the user's equipment, we propose the modified power control scheme consisting of the modified closed-loop and open-loop power control. The modified CLPC (closed-loop power control) algorithm, combining the delay compensation algorithms and pilot diversity, is mainly applied to the ATC link in urban areas because it is more suitable to the short RTD (round-trip delay). In the case of rural areas where ATCs are not deployed or a signal is not received from ATCs, combining monitoring transmitting power equipment and OLPC (open-loop power control) algorithms using an efficient pilot diversity is mainly applied to the link between the user's equipment and the satellite. Two power control algorithms are applied equally to the boundary areas where two kinds of signals are received in order to ensure coverage continuity. The simulation results show that the modified power control scheme has good performance compared to conventional power control schemes in a GEO (geostationary earth orbit) satellite system with ATC.