A new tyrosinase biosensor based on covalent immobilizationof enzyme on N-(3-aminopropyl) pyrrole polymer film

Rajesh,K.Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polymer film of N-amino substituted pyrrole monomer has been prepared for covalent immobilization of enzyme for biosensing applications, illustrated by tyrosinase (PPO).The tyrosinase enzyme retains its bioactivity when being immobilized on N-substituted pyrrole polymer film by covalent bonding.The enzyme electrode was characterized by UV.Vis and infrared spectroscopy.Phenolic compounds were quantitatively estimated by the direct electrochemical reduction of enzymatically liberated quinone species at 0.2 V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode show sensitivity of 57.6, 71.4 and 45.8 mAM1cm2 and a linear response range of 1.8.170.2, 1.3.110.1 and 2.1.168 lM for phenol, catechol and p-cresol, respectively. The biosensor exhibits a lowest detection limit of 0.9, 0.7 and 1.1 lM, for phenol, catechol and p-cresol, respectively and a period of stable sensitivity of 3 months at 4.5 C.

Simultaneous co-immobilization of enzyme and a redox mediatorin polypyrrole film for the fabrication of an amperometricphenol biosensor

Rajesh,S.S. Pandey,W. Takashima,K. Kaneto 한국물리학회 2005 Current Applied Physics Vol.5 No.2

A conducting polypyrrole lm has been prepared on indium tin oxide coated glass plate by electropolymerization of pyrrole in anaqueous solution of enzyme, tyrosinase (PPO), and a redox mediator potassium hexacyanoferrate (II). The enzyme retains its bio-activity well within the polymer lm. Phenol concentration in aqueous solution was quantitatively estimated by monitoring the elec-tron exchange occurring in redox mediator within the polymer matrix i.e. direct reduction of Fe (III) to Fe (II) ion at +0.12V vs. Ag/AgCl. The results of amperometric response measurements conducted on enzyme electrode (PPO/Fe2+/PPY/ITO) show a sensitivityof 0.33 AM. 1 cm. 2 and a linear response range of 4.5107.4l M for phenol in aqueous medium. The electrode exhibits a responsetime of about 80 second and is stable for 4 months at 4.C..

A novel hybrid technique to treat flush mesenteric arterial occlusion in acute-on-chronic mesenteric ischemia

Rajesh Girdhardas Mundhada,Atul Dharmaraj Rewatkar,Aishwarya Atul Rewatkar,Anju Rajesh Mundhada,Nikita Navin Chandak 소화기인터벤션의학회 2023 International journal of gastrointestinal interven Vol.12 No.1

Acute mesenteric occlusion is a life-threatening condition with a significant mortality rate and requires prompt diagnosis and revascularization using open repair, a hybrid procedure, or endovascular intervention. The open approach is associated with significant morbidity and mortality, while endovascular procedures are done by the antegrade or retrograde open mesenteric stent technique and have been observed to improve the prognosis and outcomes of mesenteric ischemia. We report three cases of acute-on-chronic mesenteric ischemia secondary to occlusion with failed percutaneous mesenteric artery stenting. They were successfully treated using a novel hybrid technique, without the need for mesenteric arteriotomy or subsequent arterial repair.

Effect of Preoperative Vitamin D Levels on Functional Performance after Total Knee Arthroplasty

Rajesh Navin Maniar,Aniket Machindra Patil,Adit Rajesh Maniar,Bharat Gangaraju,Jaivardhan Singh 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.2

Background: Low vitamin D levels affects muscle function. Vitamin D and calcium deficiency cause osteomalacic myopathy and poor functional recovery after hip fractures. The relationship of vitamin D and functional performance after total knee arthroplasty (TKA) is not previously reported. Methods: Influence of vitamin D on functional performance before and after TKA was reviewed retrospectively in 120 patients. Of these, 64 had vitamin D deficiency (25-hydroxy vitamin D < 30 ng/mL) preoperatively. All 120 patients received vitamin D oral supplementation postoperatively. Functional parameters including Western Ontario and McMaster Osteoarthritis Index (WOMAC), Short-Form Health Survey questionnaire, and Knee Society Score were assessed pre- and postoperatively at 3 months. Results: Preoperative function was significantly lower in osteoarthritic patients with vitamin D deficiency (WOMAC score; p = 0.040), but at 3 months all functional scores were similar. Conclusions: We concluded that vitamin D deficiency has a negative effect on function in knee osteoarthritic patients. However, postoperative vitamin D supplementation can lead to functional recovery in these patients. Thus, TKA should not be delayed in vitamin D deficient patients; rather supplementation in the postoperative period is preferable to achieve comparable functional outcome at 3 months to patients with vitamin D sufficiency.

Ratio-Cum-Product Estimators of Population Mean Using Known Population Parameters of Auxiliary Variates

Tailor, Rajesh,Parmar, Rajesh,Kim, Jong-Min,Tailor, Ritesh The Korean Statistical Society 2011 Communications for statistical applications and me Vol.18 No.2

This paper suggests two ratio-cum-product estimators of finite population mean using known coefficient of variation and co-efficient of kurtosis of auxiliary characters. The bias and mean squared error of the proposed estimators with large sample approximation are derived. It has been shown that the estimators suggested by Upadhyaya and Singh (1999) are particular case of the suggested estimators. Almost ratio-cum product estimators of suggested estimators have also been obtained using Jackknife technique given by Quenouille (1956). An empirical study is also carried out to demonstrate the performance of the suggested estimators.

Molecular fossils “pseudogenes” as functional signature in biological system

Rajesh Kumar Singh,Divya Singh,Arpana Yadava,Akhileshwar Kumar Srivastava 한국유전학회 2020 Genes & Genomics Vol.42 No.6

Pseudogenes have been known as non-functional molecular relics developed from inactivated genomic mutations while evolution. However, they have a various function at distinct molecular stages (DNA, RNA and protein) in different biological events including in cancer and associated with parental-gene-dependently and parental-gene independently. The interactions of pseudogenes with ancestor genes or other genes alter in their sequences and transcriptional processes. Pseudogene of RNA sequences have multiple functions in post-transcriptional activities as antisense RNAs, endogenous small-interference RNAs, and competing endogenous RNAs. Moreover, it also plays vital roles in controlling of its parent genes and other pseudogenes transcribing into RNA. Pseudogene transcripts make small interfering RNA or decline cellular miRNA level. The current review focuses on pseudogene functional signature in human genome by regulating the gene expression.

Heat Flux Measurements in High Velocity Oxygen-Fuel Torch Flow for Testing High Thermal Materials

Rajesh Kumar Chinnaraj,Seong Man Choi(최성만),Seong Min Hong(홍성민) 한국추진공학회 2020 한국추진공학회 학술대회논문집 Vol.2020 No.7

고열 재료를 시험평가 하기 위한 HVOF 흐름에서 가든 게이지(Gardon gauge)를 이용하여 열유속을 측정하였다. 노즐 출구로부터 4 개의 다른 위치에서 열유속 측정을 수행하였다. 실험 중 가든 게이지를 수냉시켰다. 유효한 가정을 적용하여 열량 열유속(Calorimetric heat flux)을 계산하고 가든 게이지의 열유속 측정 결과와 잘 일치하는 것으로 나타났으며, 따라서 서로를 검증했습니다. A Gardon gauge was used to measure heat fluxes in a HVOF flow intended for testing high thermal materials. The measurements were taken at four different locations from the nozzle exit. The Gardon gauge was water cooled during the experiments. Applying valid assumptions, the calorimetric heat fluxes were calculated and were shown to be in good agreement with Gardon gauge heat flux results, thus validating each other.

Potentiostatic deposition of CoNi₂Se₄ nanostructures on nickel foam as efficient battery-type electrodes for supercapacitors

Rajesh, John Anthuvan,Lee, Young-Hoon,Yun, Yong-Han,Quy, Vu Hong Vinh,Kang, Soon-Hyung,Kim, Hyunsoo,Ahn, Kwang-Soon Elsevier 2019 Journal of Electroanalytical Chemistry Vol.850 No.-

<P><B>Abstract</B></P> <P>Bimetallic CoNi<SUB>2</SUB>Se<SUB>4</SUB> nanostructures on nickel-foam substrates were fabricated by a potentiostatic deposition method by adjusting the deposition solution p<SUP>H</SUP>, and used as electrode materials in battery-type supercapacitors. The electrodeposition solution p<SUP>H</SUP> had an important role in the fabrication of the desirable CoNi<SUB>2</SUB>Se<SUB>4</SUB> nanostructures. At a deposition solution p<SUP>H</SUP> of 2.0, the synthesized product contained CoNi<SUB>2</SUB>Se<SUB>4</SUB> nanoparticles. In contrast, a partially converted flake-like morphology was obtained at p<SUP>H</SUP> = 2.5. The appropriate flake-like morphology was formed by increasing the deposition solution p<SUP>H</SUP> to 3.0. The robust flake-like structure facilitated the electron transport during redox reactions, providing an excellent electrochemical behavior in terms of specific capacity, rate capability, and cyclic stability. A battery-type supercapacitor based on the flake-like CoNi<SUB>2</SUB>Se<SUB>4</SUB> structure exhibited a maximum specific capacity of 632.5 C g<SUP>−1</SUP> at a current density of 1 A g<SUP>−1</SUP> and excellent rate performance (capacity retention of 91%) in the range of 1 to 40 A g<SUP>−1</SUP>. The material retained 83.31% of its initial capacity after 3000 cycles of charging/discharging at a current density of 40 A g<SUP>−1</SUP>, which suggests a high long-term cycling stability. The excellent electrochemical performance of the CoNi<SUB>2</SUB>Se<SUB>4</SUB> electrode material make it very promising for application in supercapacitors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CoNi<SUB>2</SUB>Se<SUB>4</SUB> nanostructures were fabricated by a potentiostatic deposition method. </LI> <LI> The electrodeposition solution p<SUP>H</SUP> is a key role in the electrochemical performance. </LI> <LI> The flake-like CoNi<SUB>2</SUB>Se<SUB>4</SUB> structure exhibited a maximum specific capacity of 632.5 C g<SUP>−1</SUP>. </LI> <LI> The electrode exhibited the excellent rate performance (capacity retention of 91%). </LI> </UL> </P>

Aggregation of Unit Commitment with Demand Side Management

Rajesh K.,Visali N. 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.2

Multi-objective unit commitment (MOUC) problem incorporated with price elasticity demand with the participation of residential customers in ancillary service markets having multilevel incentives using hybrid method is proposed. In this paper, the proposed hybrid method is the combination of non-dominant sorting genetic algorithm-II (NSGA-II) and population variant diff erential evolution (PVDE) algorithm. The hybrid method is implemented in scheduling thermal units for achieving an optimal value of multi-objective, namely cost and emission. Demand side management has become a key factor in compensating the unreliable situation during peak hours of load demand. Intervention of consumers in incentive based demand response programs (DRP) for diminishing of peak loads in peak times is applied. Demand reduction request (DRR) from entities insists the customers to participate in the DRP based on their comfort level of engaging air conditioners and allocates the incentives, which is benefi cial for both customers and utilities. The proposed method is examined on IEEE thirty nine bus system with ten generators and also on 17 unit system with 50, 80 and 100 percent compromise of customers in DRP.

Bio-Composites Reinforced with Natural Fibers: Comparative Analysis of Thermal, Static and Dynamic-Mechanical Properties

Rajesh Mishra,Jakub Wiener,Jiri Militky,Michal Petru,Blanka Tomkova,Jana Novotna 한국섬유공학회 2020 Fibers and polymers Vol.21 No.3

In the present study, flax, jute and glass woven fabric samples were developed as reinforcement for compositeswith bio-epoxy resin. Flax fabrics were developed with plain weave, twill weave (different areal densities) and unidirectional(UD) structure. Jute fabrics were woven with three different structures i.e. plain, matt and twill respectively. For comparisonpurpose, glass woven fabric with plain weave and 3D orthogonal weave were used in this study. Static mechanical propertieslike flexural strength and impact resistance were studied. Dynamic mechanical properties were investigated by DMA 40XTRMI equipment.. Thermal conductivity has been studied for all composite samples. It is observed that the static mechanicalproperties of glass fabrics reinforced composites are superior but the flax & jute fabric reinforced composites are moreflexible. The specific flexural strength as well as specific impact strength of the flax & jute fabric reinforced composites arecompetitive. The dynamic mechanical properties are comparable to that of glass fabric reinforced composites. They can beused in relatively lower load bearing applications as they are a sustainable source of materials for bio-composites whichreduce the environmental burden and reduce the manufacturing cost.