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Antioxidant activity of hot water extract from Hydrallgea macrophylla var. thunbergii
Ha Rin Park,Ju Eun Kim,Su Ji Park,Ha Yeon Park,Eun Seo Beak,Myo Jeong Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The aim of this study is to evaluate antioxidant activities of Hydrallgea macrophylla var. thunbergii(Sookuk tea) extracts. Hot water extract of Sookuk tea leave was fractionated serially by n-hexane, chloroform, ethyl acetate, and n-butanol. The antioxidant activity of each fraction was tested using a DPPH method. The IC50 value for scavenging 50% DPPH radicals was 2.50±0.04 mg/mL for hot water extract of Sookuk tea leave. Among the fractions, the IC50 value of ethyl acetate fraction was the lowest (0.461±0.004 mg/mL) and the residual aqueous fraction was the highest (6.30±0.30 mg/mL). The total polyphenol and flavonoid content of the ethyl acetate fraction were the highest, which supported the strong antioxidant activity of the fraction.
( Ji Won Park ),( Hyoung Su Kim ),( Seung In Seo ),( Sung Eun Kim ),( Su Rin Shin ),( Ki Tae Suk ),( Myoung Kuk Jang ),( Sang Hoon Park ),( Dong Joon Kim ),( Myung Seok Lee ),( Choong Kee Park ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) are widely used as tumor markers in hepatocellular carcinoma (HCC). The aim of this study is to determine the optimal cutoff values and to compare the diagnostic role of AFP and PIVKA-II in chronic hepatitis B (CHB) patients. Methods: A total of 1255 CHB patients, including 157 patients with HCC, 879 with non-cirrhotic CHB and 219 with cirrhosis without HCC, were retrospectively enrolled. The area under the receiver operating characteristic (AUROC) curves of PIVKA-II, AFP and their combination were calculated and compared. Results: Optimal cutoff values for PIVKA-II and AFP were 40 mAU/mL and 10 ng/mL for differentiating HCC from nonmalignant CHB, respectively, on ROC curve. The sensitivity and specificity were 73.9% and 89.7% for PIVKA-II, 67.5% and 90.3% for AFP, respectively. The AUROC curves of both PIVKA- II and AFP were not significantly different (0.854 vs. 0.853, P=0.965) for differentiating HCC from nonmalignant CHB, whereas the AUROC of PIVKA-II was significantly better than that of AFP in cirrhotic patients (0.870 vs. 0.812, P=0.042). When PIVKA-II and AFP were combined, diagnostic power improved significantly compared to either AFP or PIVKA-II alone for differentiating HCC from nonmalignant CHB (P<0.05), especially in cirrhosis (P<0.05) and even early HCC from LC (P<0.05). Conclusions: Serum PIVKA-II, at the level of 40 mAU/mL, is a better tumor marker in CHB patients, especially cirrhotic patients, than AFP and the combination with AFP may enhance early detection of HCC in CHB patients.
Baek, Ae Rin,Lee, Ji Min,Seo, Hyun Jung,Park, Jong Sook,Lee, June Hyuk,Park, Sung Woo,Jang, An Soo,Kim, Do Jin,Koh, Eun Suk,Uh, Soo Taek,Kim, Yong Hoon,Park, Choon Sik The Korean Academy of Tuberculosis and Respiratory 2016 Tuberculosis and Respiratory Diseases Vol.79 No.3
Background: Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal lung disease characterized by the accumulation of excessive fibroblasts and myofibroblasts in the extracellular matrix. The transforming growth factor ${\beta}1$ (TGF-${\beta}1$)-induced epithelial-to-mesenchymal transition (EMT) is thought to be a possible source of fibroblasts/myofibroblasts in IPF lungs. We have previously reported that apolipoprotein A1 (ApoA1) has anti-fibrotic activity in experimental lung fibrosis. In this study, we determine whether ApoA1 modulates TGF-${\beta}1$-induced EMT in experimental lung fibrosis and clarify its mechanism of action. Methods: The A549 alveolar epithelial cell line was treated with TGF-${\beta}1$ with or without ApoA1. Morphological changes and expression of EMT-related markers, including E-cadherin, N-cadherin, and ${\alpha}$-smooth muscle actin were evaluated. Expressions of Smad and non-Smad mediators and TGF-${\beta}1$ receptor type 1 ($T{\beta}RI$) and type 2 ($T{\beta}RII$) were measured. The silica-induced lung fibrosis model was established using ApoA1 overexpressing transgenic mice. Results: TGF-${\beta}1$-treated A549 cells were changed to the mesenchymal morphology with less E-cadherin and more N-cadherin expression. The addition of ApoA1 inhibited the TGF-${\beta}1$-induced change of the EMT phenotype. ApoA1 inhibited the TGF-${\beta}1$-induced increase in the phosphorylation of Smad2 and 3 as well as that of ERK and p38 mitogen-activated protein kinase mediators. In addition, ApoA1 reduced the TGF-${\beta}1$-induced increase in $T{\beta}RI$ and $T{\beta}RII$ expression. In a mouse model of silica-induced lung fibrosis, ApoA1 overexpression reduced the silica-mediated effects, which were increased N-cadherin and decreased E-cadherin expression in the alveolar epithelium. Conclusion: Our data demonstrate that ApoA1 inhibits TGF-${\beta}1$-induced EMT in experimental lung fibrosis.
Multi-spatial-frequency and phase-shifting profilometry using a liquid crystal phase modulator
Joo, Kyung-Il,Park, Chang-Sub,Park, Min-Kyu,Park, Kyung-Woo,Park, Ji-Sub,Seo, Youngmin,Hahn, Joonku,Kim, Hak-Rin The Optical Society 2012 Applied optics Vol.51 No.14
<P>Optical profilometry is widely applied for measuring the morphology of objects by projecting predetermined patterns on them. In this technique, the compact size is one of the interesting issues for practical applications. The generation of pattern by the interference of coherent light sources has a potential to reduce the dimension of the illumination part. Moreover, this method can make fine patterns without projection optics, and the illumination part is free of restriction from the numerical aperture of the projection optics. In this paper, a phase-shifting profilometry is implemented by using a single liquid crystal (LC) cell. The LC phase modulator is designed to generate the interference patterns with several different spatial frequencies by changing selection of the spacing between the micro-pinholes. We manufactured the LC phase modulator and calibrated it by measuring the phase modulation amount depending on an applied voltage. Our optical profilometry using the single LC cell can generate multi-spatial frequency patterns as well as four steps of the phase-shifted patterns. This method can be implemented compactly, and the reconstructed depth profile is obtained without a phase-unwrapping algorithm.</P>
Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3
Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.
Park, Mi-Young,Kim, Young-Eui,Seo, Myong-Rang,Lee, Jae-Rin,Lee, Chan Hee,Ahn, Jin-Hyun American Society for Microbiology 2006 Journal of virology Vol.80 No.6
<B>ABSTRACT</B><P>Four phosphoproteins, of 34, 43, 50, and 84 kDa, with common amino termini are synthesized via alternative splicing from the UL112-113 region of the human cytomegalovirus genome. Although genetic studies provided evidence that both the UL112 and UL113 loci in the viral genome are required for efficient viral replication, whether the four proteins play specific roles or cooperate in replication is not understood. Here we present evidence, using in vitro and in vivo coimmunoprecipitation assays, that the four UL112-113 proteins both self-interact and interact with each other. A mapping study of the 84-kDa protein showed that the N-terminal region encompassing amino acids 1 to 125, which is shared in all UL112-113 proteins and highly conserved among betaherpesviruses, is required for both self-interaction and nuclear localization as foci. Further localization studies revealed that, unlike the 43-, 50-, and 84-kDa proteins, which were distributed as nuclear punctate forms, the 34-kDa form was located predominantly in the cytoplasm. However, when all four proteins were coexpressed simultaneously, all of the UL112-113 proteins were efficiently localized to the promyelocytic leukemia oncogenic domains. We also found that the ability of the UL112-113 proteins to relocate UL44 (the viral polymerase processivity factor) to prereplication foci relied on self-interaction and reached maximal levels when the four proteins were coexpressed. Therefore, our data suggest that interactions occurring among UL112-113 proteins via their shared N-terminal regions are important to both their intranuclear targeting and the recruitment of UL44 to subnuclear sites for viral replication.</P>