Preparation of Thermo-Responsive and Injectable Hydrogels Based on Hyaluronic Acid and Poly(N-isopropylacrylamide) and Their Drug Release Behaviors

Ha Dong In,Lee Sang Bong,Chong Moo Sang,Lee Young Moo,Kim So Yeon,Park Young Hoon The Polymer Society of Korea 2006 Macromolecular Research Vol.14 No.1

Copolymers composed of hyaluronic acid (HA) and poly(N-isopropylacrylamide) (PNIPAAm) were prepared to create temperature-sensitive injectable gels for use in controlled drug delivery applications. Semi-telechelic PNIPAAm, with amino groups at the end of each main chain, was synthesized by radical polymerization using 2-aminoethanethiol hydrochloride (AESH) as the chain transfer agent, and was then grafted onto the carboxyl groups of HA using carbodiimide chemistry. The result of the thermo-optical analysis revealed that the phase transition of the PNIPAAm-grafted HA solution occurred at around 30$\∼$33$^{circ}C$. As the graft yield of PNIPAAm onto the HA backbone increased, the HA-g-PNIPAAm copolymer solution exhibited sharper phase transition. The short chain PNIPAAm-grafted HA ($M_{w}$=6,100) showed a narrower temperature range for optical turbidity changes than the long chain PNIPAAm-grafted HA ($M_{w}$=13,100). PNIPAAm-grafted HA exhibited an increase in viscosity above 35$^{circ}C$, thus allowing the gels to maintain their shape for 24 h after in vivo administration. From the in vitro riboflavin release study, the HA-g-PNIPAAm gel showed a more sustained release behavior when the grafting yield of PNIPAAm onto the HA backbone was increased. In addition, BSA released from the PNIPAAm-g-HA gels showed a maximum concentration in the blood 12 h after being injected into the dorsal surface of a rabbit, followed by a sustained release profile after 60 h.

생태계서비스 기반 국립공원 생태복지 개념정립 및 정책방향 설정

박은하 ( Eun Ha Park ),최수정 ( Su Jung Choi ),오충현 ( Choong Hyeon Oh ),정부희 ( Boo Hee Jung ),이나연 ( Na Yeon Lee ) 한국환경생태학회 2016 한국환경생태학회지 Vol.30 No.2

생태복지는 인간의 복지와 건강한 생태계의 상호관계성에 대한 국제적 담론에서 시작되었다. 본 연구는 국립공원이 생태복지의 최대 실현가능 지역이라는 점에서 국립공원에 적합한 ‘국립공원 생태복지’ 개념을 정립하고 정책방향을 설정함으로써 향후 국립공원에서의 생태복지 실현을 도모하기 위한 기초자료를 제공하는데 그 목적이 있다. 이를 위해 사전워크숍과 총 2회에 걸친 델파이 조사를 수행하였다. 본 연구를 통해 ‘국립공원 생태복지’는 국립공원에서 생태계의 지속가능성과 생물다양성을 유지하며, ‘국립공원 인간복지’와 ‘국립공원 생태계복지’의 조화를 지향하는 복지로 정의되었다. 2차 델파이 결과 모든 항목에서 평균 4.00 이상, 내용 타당도(CVR) 0.37 이상, 변이계수(CV)0.5 미만으로 타당한 내용으로 확인되었다. 국립공원 생태복지의 정책방향을 설정하기 위해 생태복지의 바탕이 되는 생태계서비스의 요소들 중 국립공원에 적합한 주요 요소를 도출한 결과 공급서비스에서 먹이·식량, 물, 유전자원 3항목, 조절서비스와 기반(서식지)서비스의 모든 항목, 문화서비스에서 미적정보, 휴양관광, 심신치유, 지식체계ㆍ교육적가치 4항목이 최종 도출되었다. 또한 본 연구에서는 국립공원 생태복지의 개념과 향후 국립공원 생태복지 정책이 어떻게 나아가야할지 방향성을 제시하였다. 그러나 이를 실현하기 위해서는 향후 이에 대한 구체적인 단위사업에 대한 실행계획이 수반되어야 하며 이를 위한 추가적인 연구가 필요할 것이다. The concept of Eco-welfare emerged in the process of discovering the relationship between a healthy ecosystem and human wellbeing. The objective of this study is to offer basic data for eco-welfare policy realization by conceptualizing the ‘National Park Eco-welfare’ since national parks are appropriate places for eco-welfare to be optimally implemented. A pre-workshop and two rounds of Delphi surveys were conducted to determine the concept of the ‘National Park Eco-welfare ’ and the main elements to be included in ecosystem service. Through this study, the concept of ‘National Park Eco-welfare’ was defined as efforts to conserve the biodiversity and sustainability of the ecosystem and create harmony between ‘National Park Ecosystem Welfare’ and ‘National Park Human Welfare’. To establish the direction of the National Park Eco-welfare policy, we identified the main elements of ecosystem services which are appropriate for the National Park. These are comprised of 3 elements (food, fresh water and genetic resources) from supply service, all elements from regulating service and supporting/habitat service and 4 elements (aesthetic information, recreation-based ecotourism, healing, and knowledge systems with educational values) from culture service. In this study, the concept of National Park Eco-welfare was established, and its policy objectives and scope were suggested. However, further studies are necessary to develop action plans areand thereby realize the policy.

Valuation of ecosystem services in the organic carbon of the Pinus densiflora forest at Mt. Namsan, Seoul Metropolitan City

Lee, Eung-Pill,Lee, Soo-In,Jeong, Heon-Mo,Han, Young-Sub,Lee, Seung-Yeon,Park, Jae-Hoon,Jang, Rae-Ha,Hong, Youg-Sik,Jung, Young-Ho,Kim, Eui-Joo,Lee, Sang-Hun,You, Young-Han The Ecological Society of Korea 2019 Journal of Ecology and Environment Vol.43 No.4

Background: Standing biomass, litter production, rate of litter decomposition, amount of litter on forest floor, and amount of soil organic carbon distribution were investigated from March 2016 to November 2018 in order to evaluate the ecosystem value through organic carbon distribution in the Pinus densiflora forest at Namsan, Seoul Metropolitan City. Results: The amount of organic carbon in the Pinus densiflora forest was 261.09 ton C ha^-1 during the research period, and fixed organic carbon in plant through net photosynthesis was 3.2 ton C ha^-1 year^-1. The organic carbon in plant was 62.77 ton C ha^-1 (24.04%), in litter on the forest floor was 3.65 ton C ha^-1 (1.40%), and in soil was 194.67 ton C ha^-1 (74.56%). The value of plant, litter on forest floor, and soil organic carbon distribution were each 6,277,000 won ha^-1, 365, 000 won ha^-1, and 19,467,000 won ha^-1. The value per ton of fixed organic carbon in plant through net photosynthesis was 320,000 won ha^-1 year^-1 and the value of ecosystem services stored in carbon in the Namsan forest ecosystem was about 26.1 million won ha^-1 for 3 years. Conclusions: The results of this study suggest that the pine forests of Namsan rapidly decompose leaves due to the high atmospheric temperature and accumulate a large amount of organic carbon in the soil to provide climate control regulatory service function.

Current Status of Ginseng Cultivation and Soil Characteristics of Northeastern Three Provinces in China

Park, Yang Ho,Kim, Jang Uk,Kim, Dong Hwi,Sonn, Yeon Kyu,Yun, Jin Ha,Moon, Huhn Pal,Cho, Soo Yeon 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

This study was conducted to improve the continuous techniques for international competitiveness of ginseng industry to Korea-China FTA negotiation and conclusion, and provide the basic information for ginseng industry development of Korea. It was carried out the visiting of the northeastern three provinces (Jilin, Liaoling and Heilongjang) in China for 3-year from 2014 to 2016 and observed the farmers' fields of ginseng cultivation with soil environmental status. The types of ginseng cultivation could be observed in small scales of 0.5~3.0 ha, in middle scales of 4.0~10.0 ha and in large scales of 30~700 ha with the kinds of imhasam, Chinese ginseng, Korean ginseng and western ginseng. Also ginseng was cultivated in newly reclaimed land of forest in two types of direct seeding and transplanting of ginseng seedlings. The field beds of ginseng growing were covered with vinyl films in arch design of 100~130 cm height and vinyl was painted in spraying with blue, green and yellow colours for shading. It was investigated in status of the physico-chemical properties of soils. The physical information on the field soils were silt loam, loam and sandy loam in soil textures, and some plain in low slope, some alluvial fan or local valley in forest of land topography. Soil pH ranged within 5.0~5.2, soil EC was $0.93{\sim}3.78dS\;m^{-1}$, organic matter was $37{\sim}35g\;kg^{-1}$, nitrate nitrogen $63{\sim}490mg\;kg^{-1}$, available $P_2O_5$ $55{\sim}163mg\;kg^{-1}$, and in exchangeable cations, K was 0.30~0.98, Ca was 6.5~14.0, Mg was $1.1{\sim}5.3cmol_c\;kg^{-1}$ in ranges. Farmers used the fertilizer for ginseng cultivation in 10~11 t of compost, $200{\sim}400kg\;ha^{-1}$ of complex fertilizer and $750kg\;ha^{-1}$ of oil cakes. The northeastern three provinces of China can use the newly lands with large areas of ginseng cultivation in soil sickness by continuous cropping. and the soil basic fertility is batter than that of Korean in standard guide of ginseng cultivation soil.

Current Status of Ginseng Cultivation and Soil Characteristics of Northeastern Three Provinces in China

Yang Ho Park,Jang Uk Kim,Dong Hwi Kim,Yeon Kyu Sonn,Jin Ha Yun,Huhn Pal Moon,Soo Yeon Cho 한국토양비료학회 2016 한국토양비료학회지 Vol.49 No.6

This study was conducted to improve the continuous techniques for international competitiveness of ginseng industry to Korea-China FTA negotiation and conclusion, and provide the basic information for ginseng industry development of Korea. It was carried out the visiting of the northeastern three provinces (Jilin, Liaoling and Heilongjang) in China for 3-year from 2014 to 2016 and observed the farmers’ fields of ginseng cultivation with soil environmental status. The types of ginseng cultivation could be observed in small scales of 0.5∼3.0 ha, in middle scales of 4.0∼10.0 ha and in large scales of 30∼700 ha with the kinds of imhasam, Chinese ginseng, Korean ginseng and western ginseng. Also ginseng was cultivated in newly reclaimed land of forest in two types of direct seeding and transplanting of ginseng seedlings. The field beds of ginseng growing were covered with vinyl films in arch design of 100∼130 cm height and vinyl was painted in spraying with blue, green and yellow colours for shading. It was investigated in status of the physico-chemical properties of soils. The physical information on the field soils were silt loam, loam and sandy loam in soil textures, and some plain in low slope, some alluvial fan or local valley in forest of land topography. Soil pH ranged within 5.0∼5.2, soil EC was 0.93∼3.78 dS m<SUP>-1</SUP>, organic matter was 37∼35 g kg<SUP>-1</SUP>, nitrate nitrogen 63∼490 mg kg<SUP>-1</SUP>, available P2O5 55∼163 mg kg-1, and in exchangeable cations, K was 0.30∼0.98, Ca was 6.5∼14.0, Mg was 1.1∼5.3 cmolc kg<SUP>-1</SUP> in ranges. Farmers used the fertilizer for ginseng cultivation in 10∼11 t of compost, 200∼400 kg ha<SUP>-</SUP>1 of complex fertilizer and 750 kg ha<SUP>-1</SUP> of oil cakes. The northeastern three provinces of China can use the newly lands with large areas of ginseng cultivation in soil sickness by continuous cropping. and the soil basic fertility is batter than that of Korean in standard guide of ginseng cultivation soil.

Distribution of the Fairy Pitta (Pitta nympha) in the South Korea: A focus on protected areas

Gil-Pyo Hong(홍길표),Jae-Hoon Kim(김재훈),Seul-Gi Seo(서슬기),So-Yeon Cho(조소연),Bo-Yeon Hwang(황보연),Jong-Hyun Park(박종현),Seung-Yeon Lee(이승연),Ga-Young Kim(김가영),Wan-Hee Nam(남완희),Ha-Cheol Sung(성하철) 한국조류학회 2021 한국조류학회지 Vol.28 No.2

국내 팔색조(Pitta nympha) 분포 현황을 알아보고자 전국 국립공원 등 보호지역 중심으로 총 19개 지역을 조사하였고, 전문가 네트워크를 통해 자료를 보강하였다. 전국 19개 지역을 조사한 결과 북한산, 지리산 등 내륙지역 7개 국립공원에서는 팔색조가 확인되지 않았고, 경남 거제, 남해, 해남, 장흥 고흥 등 남해안과 인접한 12개 지역에서 팔색조 서식이 확인되었다. 또한 전문가 네트워크를 통해 수집한 팔색조 출현 위치 정보 82건을 분석한 결과, 전국 8개 광역지자체 모두 팔색조 서식이 확인되었고, 내륙지역(26.8%)보다 해안지역(73.2%)에서 더 많이 출현하였다. 연구 결과를 종합해보면, 우리나라의 팔색조는 주로 제주도와 남해안 지역을 중심으로 많이 분포하며, 그 보다 적은 개체수가 내륙 지역에 넓게 분포하고 있는 것으로 나타났다. The present study investigated the current distribution of Fairy Pitta (Pitta nympha) in South Korea. A total of 19 study areas, including protected areas such as national and provincial parks in South Korea were surveyed, and additional data obtained from an expert network. According to the results, Fairy Pitta was absent in seven inland national parks, including Mt. Bukhan and Chiri parks. Fairy Pitta inhabited 12 areas near the southern coast of South Korea, including Geoje, Namhae, Haenam, Jangheung, Goheung, and Gyeongnam Province. The analysis results of 82 datasets obtained from experts with regard to the locations where Fairy Pitta have been spotted showed that the bird inhabits all the eight metropolitan regions in South Korea, and is more frequently spotted in coastal areas (73.2%) than in inland areas (26.8%). The findings demonstrate that Fairy Pitta is mainly distributed in Jeju Island and the southern coast of South Korea, and is distributed in inland areas at relatively lower numbers.

◇ 5분과 : 아름다운 산림풍경 유형의 선호도 및 이미지 특성에 관한 연구

이연희 ( Yeon Hee Lee ),박찬우 ( Chan Woo Park ),하시연 ( Si Yeon Ha ) 한국임학회 2015 한국산림과학회지 Vol.104 No.4

본 연구는 국립공원의 아름다운 풍경사진을 모집단으로 분류한 22개의 아름다운 산림풍경 유형을 유형별 사진의 매수 비율과 인문자원 유형의 제외 등을 통해 선발한 10개의 풍경유형에 대한 선호경향 도출과 이미지 분석을 위해 실시되었다. 풍경유형의 선호도 평가는 편차치(Z척도)와 일대비교법을 사용하였고, 풍경유형의 이미지 분석은 SD법을 사용하였다. 본 연구의 결과를 요약하면 다음과 같다. 첫째, 편차치와 일대비교법에 의한 풍경유형의 선호도 평가 결과, 각 풍경유형의 요소 및 특성이 다름에도 불구하고 대체로 유사한 경향을 나타내어 평가 결과의 일관성을 확인할 수 있었다. 분석 결과, 임내풍경, 계곡과 바위와 같은 풍경에 대한 선호도가 높게 나타났고, 다음으로 능선 풍경, 기암봉우리의 암릉, 폭포와 절벽 풍경에 대한 선호도가 높았다. 한편 고사목 군락의 풍경은 가장 낮게 평가되었다. 둘째, SD법에 의한 풍경유형의 이미지 평가 결과, 요인 1 ‘신비로운 매력’, 요인 2 ‘화려한 색감’, 요인 3‘생동감·다양함’으로 구분되었다. 각 요인에 대한 풍경유형의 분포를 살펴보면 ‘신비로운 매력’의 이미지는 능선 풍경으로부터, ‘화려한 색감’의 이미지는 색으로 표현되는 계절감으로부터, ‘생동감·다양함’의 이미지는 물과 단풍, 기암봉우리의 동적이고 다양한 변화로부터 기인한 것으로 확인되었다. This study intends to evaluate visual preference and image for 10 types of forest sceneries which have own distinct characteristics without overlapping each other among 22 types of beautiful forest sceneries based on Korea National Parks. Z scale and paired comparison methods were used to analyze visual preference for forest scenery, and SD scale method was also adopted to assess visual image for forest scenery. Experiments were conducted with 3 different university students groups. The results of this study can be summarized as follows. First, the evaluation results of Z scale (N=70) and paired comparison (N=64) methods show similar visual preference for different types of forest scenery. ‘Scenery inside forests’ and ‘valley and rock’ sceneries have the highest level of preference, and ‘panorama of ridges’, ‘ridge of curious rock peaks’, and ‘waterfall and cliff’ also have relatively higher preference level than others. However, ‘community of dead trees’ has the lowest preference in forest sceneries. Second, the factor analysis outputs of the image scores for beautiful forest scenery types by SD scale (N=66) show 3 factors of ‘mysterious charm’, ‘colorful sense’, and ‘vividness/ variety’. Examined the variables of forest scenery types for each factor, the image of ‘mysterious charm’ is based on the sceneries of mountain ridges, the image of ‘colorful sense’ is focused on the sceneries of colorexpressed sense of the season, and the image of ‘vividness/variety’ is based on the sceneries of waterscape and curious rock peaks.

분포형 토양침식지도를 이용한 한강상류지역 토양유실 위험성 평가

박찬원(Chan-Won Park),손연규(Yeon-Kyu Sonn),장용선(Yong-Seon Zhang),홍석영(S. Young Hong),현병근(Byung-Keun Hyun),송관철(Kwan-Cheol Song),하상건(Sang-Keun Ha),문용희(Young-Hee Moon) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.6

본 연구에서는 1:25,000 정밀토양도 등 가용한 공간자료를 이용하여 작성된 토양유실도를 이용하여 한강 상류지역 10개 중권역 108 소유역에 대하여 토양유실량 평가하고 토지이용별 토양유실 위험지역을 분석하였다. 대상유역 총 토양유실량은 895만 Mg yr<SUP>-1</SUP>으로 예측되었으며, 면적당 평균 토양유실량은 6.1 Mg ha<SUP>-1</SUP>yr<SUP>-1</SUP>이었다. 중소유역 단위로 살펴보면 남한강지역이 북한강지역의 유실량 뿐만 아니라 면적당 유실량이 더 많았다. 이는 북한강 권역의 경우 화강암 및 화강편마암이 주요 모재인 반면에 남한강 권역의 경우 토양모재가 퇴적암지대를 많이 포함있어 토양침식성 인자 (K factor)와 경사 인자 (LS factor)가 상대적으로 높고, 남한강 지역의 경우 농경지 토양이 상대적으로 많이 분포하고 있기 때문으로 판단된다. 대상유역의 토지이용별 연평균 토양유실량을 분석한 결과 산림/초지 > 밭 >> 도심/대지 >> 논 > 과수의 순로 분석 대상유역 중 10.7%를 차지하고 있는 농경지에서 유실되는 토양의 추정량은 41.3%이였으며, 이중 6.2%면적을 차지하는 밭이 40.6%으로 44.2%의 산림/초지와 비슷한 수준이었다. 한강상류지역 토양유실 위험성 평가시 분포형 토양침식지도와 항공사진을 비교한 결과 토양유실에 대한 공간인 정보를 확실하게 보여주고 있으며, 토양침식위험성은 남한강권역의 남한강 상류 (1001), 평창강 (1002), 충주댐 (1003) 권역에서 각각 토양침식위험성이 “보통”이상으로 높은 지역이 각각 8.7%, 7.9%, 7.8%로 평균인 5.9%보다 높았으며, 이에 대한 보전방안이 강구될 필요가 있고 판단된다. This study was conducted to evaluate soil erosion risk with a standard unit watershed in the upper Han river basin using the spatial soil erosion map according to the change of landuse. The study area is 14,577 ㎢, which consists of 10 subbasins, 107 standard unit watersheds. Total annual soil loss and soil loss per area estimated were 895 × 10<SUP>4</SUP> Mg yr<SUP>-1</SUP> and 6.1 Mg ha<SUP>-1</SUP> yr<SUP>-1</SUP>, respectively. A result of analysis with a subbasin as a unit showed that annual soil losses and soil loss per area in Namhan river basins was more than in Bukhan river ones. Predicted annual soil loss according to the landuse ranked as Forest & Grassland > Upland >> Urban & Fallow area > Paddy field > Orchard. Upland area covered 6.2% of the study area, but the contribution of total annul soil loss was 40.6% and that of Forest & Grassland was 44.2%. As a evaluation of soil erosion risk using the spatial soil erosion map, we could precisely conformed the potential hazardous region of soil erosion in each unit watersheds. The ratio of regions, graded as higher “Moderate” for annual soil loss, were respectively 8.7%, 7.9% and 7.8% in 1001, 1002 and 1003 subbasins in Namhan river basin. Most landuse of these area was upland, and these area is necessary to establish soil conservation practices to reduce soil erosion based on the field observation.

맥주박 바이오차 처리가 암모니아 배출량에 미치는 영향 평가

이재한(Jae-Han Lee),최하연(Ha-Yeon Choi),박성용(Seong-yong Park),천진혁(Jin-Hyuk Chun),강윤구(Yun-Gu Kang),윤여욱(Yeo-Uk Yun),이기범(Gibum Yi),오택근(Taek-Keun Oh) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11

암모니아(NH₃)는 대기 중 황산화물(SOx), 질소산화물(NOx) 등과 반응하여 황산암모늄, 질산암모늄과 같은 2차 미세먼지를 생성하는 주요 미세먼지 원인물질이다. 2017년 기준 국내에서 NH₃는 총 308,298 ton year<SUP>-1</SUP> 배출되었으며, 농업부문에서 약 79.3%의 비율로 가장 많이 배출되었다. 토양에 질소질 비료의 시용 시 발생되는 NH₃의 대기로 배출은 토양 내 질소 손실의 원인이 되기도 한다. 바이오차는 넓은 표면적과 표면의 작용기를 가지고 있어 토양 내에서 중금속이나 오염물질 등을 제거하는 특징이 있다. 본 연구는 다양한 온도에서 열분해된 맥주박 바이오차 처리가 NH₃ 배출량에 미치는 영향을 평가하기 위해 실시하였다. 바이오차는 충분히 건조된 맥주박을 300, 500, 700°C에서 각각 1시간 동안 열분해하여 제조하였다. 각 바이오차를 토양에 3% (w w<SUP>-1</SUP>)씩 혼합 후 NH₃ 포집을 위해 자체 제작한 아크릴컬럼에 작토층 높이인 20 cm 까지 토양을 채웠으며, 토양 수분함량은 20%로 조절하였다. 질소질 비료는 작물별 시비처방기준의 배추를 기준으로 요소를 각 처리구에 320 kg ha<SUP>-1</SUP>로 처리하였다. NH₃의 포집은 요소 처리 후부터 약 30일간 실험실 내에서 실시하였다. 배출되는 NH₃는 0.05 N H₂SO₄으로 포집하여, Indophenol blue method로 비색정량하였다. 그 결과, 시험 기간 중 총 NH₃ 배출량은 BB<SUB>700</SUB>처리구가 약 11.83 kg ha<SUP>-1</SUP>로 가장 높게 나타났다. BB<SUB>300</SUB>처리구의 총배출량은 약 7.34 kg ha<SUP>-1</SUP>로 가장 낮았으며, N처리구에 비해 약 30.5% 저감 되었다. BB<SUB>700</SUB>처리구는 N처리구에 비해 NH₃ 총배출량이 약 12% 증가하였는데, 이는 바이오차의 높은 pH에 의한 alkali effect로 판단된다. 실험 후 토양 분석 결과, 모든 바이오차 처리구에서 pH, EC, Av. P₂O<SUB>5</SUB>, T-C, T-N 등이 증가하였다. 특히, BB<SUB>700</SUB>처리구는 처리된 바이오차의 높은 탄소 함량으로 인하여, 토양 내 탄소 함량이 약 3.44%로 가장 높았다. 따라서, 비교적 낮은 온도에서 열분해한 BB<SUB>300</SUB>의 토양 처리가 농경지에서 배출되는 NH₃를 감축할 수 있으며, 본 연구 결과는 실제 농경지에서 배출되는 NH₃를 감축하기 위한 기초자료로 사용될 수 있을 것으로 판단된다.

구도심의 소외된 근대 유휴공간을 활용한 문화시설 리노베이션 계획 - 서울 종암동 ‘고려시장상가’부지 활용을 중심으로 -

박하연, 이상훈 충북대학교 건설기술연구소 2018 建設技術論文集 Vol.37 No.2

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