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치료 불응성 Still's병에서 자가 조혈모세포 채집시 사용된 G-CSF에 의한 급성 악화 1예
이재웅,박성현,왕준광,오호석,최정혜,배상철,이영열,김인순,최일영,안명주 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2
치료 불응성 자가면역질환에서 고용량 항암화학요법 및 자가 조혈모세포이식술이 시행되고 있으나 조혈모세포 가동화 및 이식술 시 사용되는 G-CSF에 의해 오히려 기존의 자가면역질환의 증세가 악화되었다는 보고가 있다. 저자들은 만성적으로 재발되는 Still's병 환자에서 조혈모세포 가동화를 위해 사용한 G-CSF에 의해 자가면역질환이 급성 악화된 1예를 경험하였기에 보고하는 바이다. High-dose immunosuppressive therapy with autologous stem cell transplantation is an increasingly used treatment for severe refractory autoimmune disorder including multiple sclerosis, rheumatoid arthritis, juvenile chronic arthritis, systemic lupus erythematosus. Although the optimal method of collecting stem cell is not determined, G-CSF-based mobilization is generally considered safe. However, worsening of disease status was reported in autoimmune disease undergoing mobilization with G-CSF. We report a 24-year-old male with refractory Still's disease who developed acute disease flare after administration of G-CSF for stem cell mobilization.
Survey on robotics and automation technologies for civil infrastructure
Myung, Hyun,Wang, Yang,Kang, Shih-Chung Jessy,Chen, XiaoQi Techno-Press 2014 Smart Structures and Systems, An International Jou Vol.13 No.6
Over the past several decades, substantial amounts of sensors and sensing systems have been developed for civil infrastructure systems. This special issue focuses on state-of-the-art robotics and automation technologies, including construction automation, robotics, instrumentation, monitoring, inspection, control, and rehabilitation for civil infrastructure. The issue also covers construction informatics supporting sensing, analysis and design activities needed to operate smart and sustainable civil infrastructure. Examples include robotic systems applied to civil infrastructure and equipped with various sensing technologies, such as optical sensors, laser sensors, wireless sensors, multi-sensor fusion, etc. This special issue is published in an effort to disseminate current advances of various robotics and automation technologies for civil infrastructure and built environment.
Hypoxic condition enhances chondrogenesis in synovium-derived mesenchymal stem cells
Hyun Cheol Bae,Hee Jung Park,Sun Young Wang,Ha Ru Yang,Myung Chul Lee,한혁수 한국생체재료학회 2018 생체재료학회지 Vol.22 No.4
Background: The chondrogenic differentiation of mesenchymal stem cells (MSCs) is regulated by many factors, including oxygen tensions, growth factors, and cytokines. Evidences have suggested that low oxygen tension seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in various MSCs. Recent studies report that synovium-derived mesenchymal stem cells (SDSCs) are a potential source of stem cells for the repair of articular cartilage defects. But, the effect of low oxygen tension on the proliferation and chondrogenic differentiation in SDSCs has not characterized. In this study, we investigated the effects of hypoxia on proliferation and chondrogenesis in SDSCs. Method: SDSCs were isolated from patients with osteoarthritis at total knee replacement. To determine the effect of oxygen tension on proliferation and colony-forming characteristics of SDSCs, A colony-forming unit (CFU) assay and cell counting-based proliferation assay were performed under normoxic (21% oxygen) or hypoxic (5% oxygen). For in vitro chondrogenic differentiation, SDSCs were concentrated to form pellets and subjected to conditions appropriate for chondrogenic differentiation under normoxia and hypoxia, followed by the analysis for the expression of genes and proteins of chondrogenesis. qRT-PCR, histological assay, and glycosoaminoglycan assays were determined to assess chondrogenesis. Results: Low oxygen condition significantly increased proliferation and colony-forming characteristics of SDSCs compared to that of SDSCs under normoxic culture. Similar pellet size and weight were found for chondrogensis period under hypoxia and normoxia condition. The mRNA expression of types II collagen, aggrecan, and the transcription factor SOX9 was increased under hypoxia condition. Histological sections stained with Safranin-O demonstrated that hypoxic conditions had increased proteoglycan synthesis. Immunohistochemistry for types II collagen demonstrated that hypoxic culture of SDSCs increased type II collagen expression. In addition, GAG deposition was significantly higher in hypoxia compared with normoxia at 21 days of differentiation. Conclusion: These findings show that hypoxia condition has an important role in regulating the synthesis ECM matrix by SDSCs as they undergo chondrogenesis. This has important implications for cartilage tissue engineering applications of SDSCs.
( Wang Lok Lee ),( Jun Hyun Jeong ),( Hee Geun Park ),( Youn Gran Lee ),( Soon Mi Kwon ),( Ha Kyoung Jang ),( Jong Kui Jun ),( Myung Hwa Kim ),( Sang Ki Lee ),( Kwang Mu Lee ) 한국운동영양학회 2012 Physical Activity and Nutrition (Phys Act Nutr) Vol.16 No.3
This study investigated the effect of moderate exercise training on macrophage immunocompetence in high fat diet-induced obese mice. To determine the differential effects of exercise training and low fat diet on macrophage, C57BL/6 male mice (4 week old, n=32) were fed high-fat diet for 5 weeks to induce obese. The high fat-induced obese mice were then divided into four groups and differently treated by high/low fat diet and exercise training for a further 8 weeks; HHC (13 weeks high-fat diet), HHE (13 weeks high-fat diet with 8 weeks Exercise training) and HLC (5 weeks high-fat diet and 8 weeks low-fat diet), HLE (5 weeks high-fat diet and 8 weeks low-fat diet with exercise training). The exercise groups were performed on a motorized treadmill, running for 30-60 min/day at 10-22 m/min, 0% grade for 8wks. Body weight was significantly decreased for those with a low-fat diet and exercise training (HLE). Macrophage phagocytosis was significantly increased by low-fat diet and exercise training (HLE). The percentage of stimulation of NO release induced by LPS was higher in HLE than in other groups. The percentage of stimulation of IL-1β release induced by LPS was higher in HHE and HLE than in other groups. The percentage of stimulation of IFN-γ release induced by LPS was higher in HLE than in other groups. These findings suggest that moderate exercise and low-fat diet have beneficial effects on macrophage immunocompetence in high fat diet-induced obese mice.
Anti-Proliferative Effect of Ethanol on Normal and Cancer Cells
Myung-Ju Oh(오명주),Jihyun Kim(김지현),Su-Hyun Park(박수현),Young-Hwa Jeong(정영화),Kun Wang(왕쿤),Byung-Wook Cho(조병욱),Byung H. Jhun(전병학) 한국생명과학회 2012 생명과학회지 Vol.22 No.4
에탄올은 사람에 대한 발암물질로 잘 알려져 있다. 또한 여러 조직이나 세포에서의 에탄올에 의한 세포증식억제효과도 잘 알려져 있다. 본 연구에서는 여러 암세포에서 에탄올에 의한 세포증식억제 효과를 조사하였는데 특히 발암원성 ras로 형질전환되거나 미세주입된 세포에서의 영향을 조사하였다. 에탄올은 여러 정상세포들의 증식을 억제하였다. 반면에 여러 암세포나 발암원성 Ras에 의한 세포증식은 억제하지 못 하였다. 또한 발암원성단백질의 세포내 미세주사에 의한 DNA합성 유도도 에탄올에 의해 억제 되지 않았다. 이러한 에탄올의 세포증식 억제 효과는 N-acetylcysteine이나 4-methylpyrazole과 같은 항산화제에 의해 제거되었다. 이러한 실험 결과는 에탄올에 의한 세포증식억제 효과는 Ras단백질의 upstream에 있거나 또는 Ras와 독립적으로 작용하며, 활성산소 형성과 밀접한 관계가 있다는 것을 알려준다. Ethanol is known as being carcinogenic to humans. In addition, the anti-proliferative effects of ethanol have been described for a variety of tissues and cells. In this study, we investigated the anti-proliferative effects of ethanol on various cancer cells, particularly on oncogenic ras-transformed or -injected cells. Ethanol treatment inhibited the cell proliferation of normal control cells, but did not suppress the proliferation of various cancer cells and oncogenic ras-transformed cells. Furthermore, ethanol treatment did not interfere with DNA synthesis, which was induced by microinjecting the oncogenic H-RasV12 protein. The anti-proliferative effect of ethanol was rescued by antioxidants, such as N-acetylcysteine and 4-methlpyrazole. These results suggest that ethanol cytotoxicity is exerted through free radical formation, and that the anti-proliferative action site of ethanol cytotoxicity either lies upstream, or is independent of Ras.
Hahm, Myung Gwan,Wang, Hailong,Jung, Hyun Young,Hong, Sanghyun,Lee, Sung-Goo,Kim, Sung-Ryong,Upmanyu, Moneesh,Jung, Yung Joon RSC Pub 2012 Nanoscale Vol.4 No.11
<P>High-density carbon nanotube networks (CNNs) continue to attract interest as active elements in nanoelectronic devices, nanoelectromechanical systems (NEMS) and multifunctional nanocomposites. The interplay between the network nanostructure and its properties is crucial, yet current understanding remains limited to the passive response. Here, we employ a novel superstructure consisting of millimeter-long vertically aligned single walled carbon nanotubes (SWCNTs) sandwiched between polydimethylsiloxane (PDMS) layers to quantify the effect of two classes of mechanical stimuli, film densification and stretching, on the electronic and thermal transport across the network. The network deforms easily with an increase in the electrical and thermal conductivities, suggestive of a floppy yet highly reconfigurable network. Insight from atomistically informed coarse-grained simulations uncover an interplay between the extent of lateral assembly of the bundles, modulated by surface zipping/unzipping, and the elastic energy associated with the bent conformations of the nanotubes/bundles. During densification, the network becomes highly interconnected yet we observe a modest increase in bundling primarily due to the reduced spacing between the SWCNTs. The stretching, on the other hand, is characterized by an initial debundling regime as the strain accommodation occurs via unzipping of the branched interconnects, followed by rapid rebundling as the strain transfers to the increasingly aligned bundles. In both cases, the increase in the electrical and thermal conductivity is primarily due to the increase in bundle size; the changes in network connectivity have a minor effect on the transport. Our results have broad implications for filamentous networks of inorganic nanoassemblies composed of interacting tubes, wires and ribbons/belts.</P>
자가 발반사요법이 요실금 여성의 증상과 자존감 및 우울에 미치는 영향
강현숙 ( Hyun Sook Kang ),김원옥 ( Won Ock Kim ),왕명자 ( Myung Ja Wang ) 경희대학교 동서간호학연구소 2004 동서간호학연구지 Vol.9 No.1
Purpose: This study was to identify the effects of self-foot reflexology on the situation score of Urinary incontinence, Self esteem and Depression of the middle-aged women. Method: A quasiexperimental design was used. The subject were 39 middle-aged women with urinary incontinence(18 experimental group, 21 control group). In the experimental group, self-foot reflexology was applied for 30 minutes, three times a week for 4 weeks. The obtained data were analyzed by using the Mann-Whitney U test of SPSS. Result: 1. The situation score of urinary incontinence were reduced significantly in the experimental group as compared to the control group(U=81.00, P=.002). 2. Self esteem were improved significantly in the experimental group as compared to the control group(U=59.00, p=.000). 3. Depression in the experimental group was reduced whereas that of the control group was increased significantly(U=45.00, p=.000). Conclusion: These findings indicate that self-foot reflexology is an effective method for reducing urinary incontinence and depreesion, and improving self esteem of middle-aged women. Therefore self-foot reflexology can be considered as a independent nursing intervention for urinary incontinence.