Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

무술 스포츠로서의 택견의 정신에 대한 고찰

최무현,김동준 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

Accroding to results about the mental a of Takkyun as a martial sport, We can make following conclusions. 1. The modern martial arts are the concept which is added to sports, When we indicated one of them, we use the word, "martial sports". 2. The modern Korean martial ideologies were under the influence of Japanese martail as they are, Japeneses martail ideologies are hierarchism but Korean martial characteristic are democritism. 3. Takkyun keeps Korean essential martial values like them. 4. Takkyun has been industrialized all over the world from long time ago. That is, Takkyun is one of the martial sports. 5. The martial mental of Takkyun has only mutual coexisting and democratic values which are based on the liberty and equality.

Baculovirus Vector System에 의해 발현된 재조합 Pseudorabies Virus Major Capsid Protein의 면역원성

전무형,안동준,장경수,조용성,박종현,송재영,현방훈,안수환 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-

The recombinant pseudorabies virus major capsid protein (rMCP) was produced by expression of the MCP gene in Sf-9 cell using baculovirus transfer vector system. Following evaluation of the immunochemical properties of the rMCP, the immunogenicity of the recombinant subunit protiens were investigated in guinea pig and swine to obtain the preliminary guide line for the subunit vaccine using rMCP and gP50. It was proved that ultrasonication and 30% ammonium sulfate was most efficient to concentrate and purify the protein. The rMCP was safe in mice, guinea pigs and piglets. In guinea pigs, rMCP mixed with various adjuvants induced substantial degree of serum neutralizing antibody titers, but revealed incomplete protecivity against challenge. In swine, the combination of rMCP and gP50 showed the higher serum neutralizing antibody titers and cellular immune responses than rMCP alone. However, the protectivity was lower in comparison with the commercial gI-deleted inactivated vaccine. We expect these results to contribute to characterization of MCP gene of Korean isolate of PRV and to ultilize as preliminary information for prodution and evaluation of PRV recombinant subunit vaccines.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

10일간의 크레아틴 섭취에 의해 24시간에 걸친 소변의 크레아틴 배설에 대한 고찰

최무현 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.4

The purpose of this study was to evaluate the amount of creatine present in the urine of exercising athletes ingestion with a dosage specific to their lean body tissue and at an amount approximating the maintenance dosage recommended by distributors of creatine ingestions. Male subjects(n=32) who were weight lifters(n=16) and soccer players(n=16) between the age of 19 and 25 years served for this study. In a double-blind, ramdomzed block design, 8 weight lifters and 8 soccer players were assigned to either the creatine ingestion or placebo group. As the method of urine analysis, high-performance liquid chromatography (HPLC) were carried out on a Gilson HPLC with HPLC system controller software. An SPSS-pc 2-way ANOVA procedure was used to determine if significant differences occurred between the ingestion and placebo groups for creatine and pre-to post changes. The conclusions obtained from this study were as follows: 1. In 24-hour, 48% of the ingested creatine was excreted with urine. 2. When ingesting with dosages of 0.1g/㎏ lean body mass or between 7~8g at a time, about half or the ingested creatine got excreted.

원발성 폐암 환자의 생존율에 관한 보고

김현태,이상무,어수택,박춘식,정성환,허승재,남충희,강창희,김용훈 순천향대학교 1994 논문집 Vol.17 No.4

We analysed 404 patients with primary lung carcinoma who were treated at Soonchunhyang University Hospital from July, 1985 to september, 1993 in order to investigate the survival rate and epidemiolgical properties of primary lung cancer. They were 330 males and 74 females. The most prevalent decade was seventh. In terms of cell type, the squamous cell was 225 patients (55%), and adenocarcinoma, small cell, mixed type was 21%, 19%, 4%, respectively. Among non-small cell lung carcinoma, stage Ⅲa was the most prevalent one(92%). In case of small cell carcinoma, the limited stage was 64%. The 12-, 24-, 36- month survival rate of total patients was 57%, 31%, 22%, respectivley and median sruvival time was 15 months. The 36-month survival rate tended to be longer in non-small cell lung carcinoma than that of small cell lung cancer, but there was no difference between two groups, statistically. In non-small cell carcinoma, The 36-month survival rate and meidan survival time were longer in the stage Ⅰ and Ⅱ than those of Ⅲa, Ⅲb, Ⅳ (80% versus 38%, 22%, 0%, p<0.05). According to involvement of lymph node, the 36-month survival rate was longer in NO and N1 than those of N2, N3 (61.9%, 48.7% versus 17.7%, 17.3%, p<0.05). In small cell carcinoma, The 36-month survival rate and median survival rate were higher and longer in limited stage than those of extensive stage(16.1% and 13 month vs 10% and 8 month, p<0.05). In conclusion, we report here the incidence of primary lung carcinoma and the survival rate of paients with primary lung carcinoma who were treated in Soonchunhyang University Hospital.

여성들의 크레아틴 섭취에 따른 근력 및 신체구성에 미치는 영향

최무현,도길명 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

The purpose of this study was to determine the effectiveness of creatine ingestion during weight training on the dynamic muscular strength and change of body composition in women athletes. Ten collegiate women majoring physical education consumed either creatine (n=6) or a placevo(n=6) for 5 weeks during off season training (20g/day for 1week and 4weeks). Strength tests performed were the 1-RM bench press and leg extension. An analysis on liver enzymes consisted of GGL and BUN. Body composition measurements were performed by skinfold caliper and body components analyzer. Conclusions obtained from this study were as follows; Oral creatine ingestion in crease muscular strength for the upper body, while did not influence body weight or lean body mass gain in women doing weight training for 5 weeks.

노동운동과 정치운동의 맥락적 의존성 : 부마민주항쟁 사례연구

주무현 경상대학교 사회과학연구소 2003 사회과학연구 Vol.21 No.-

I analyse the contextual dependency between labour movement and political movement in the case of the Busan and Masan Areas' democratic movement of 1979. This paper focuses on the concept of political and industrial opportunity structure as a determinant of labour movement an political movement. Busan and Masan Areas' democratic movement depended contextually on the growth and development of the democratic union movement of 1970s. The success of the political movement opened the political opportunity structure of labour movement of 1980 in Korea. At same time Korean labour movement was contextually determined by the political movement as such Busan and Masan Areas' democratic Movement. In conclusion, the success of labour movement will depend on how it use the political opportunity structure in Korea.

抗콜린劑 投與 中斷後 藥物誘發 錐體外路 症候群에 對한 Amantadine의 抗파킨슨 效果에 關한 臨床的 硏究

金武鎭,李榮料,金昌鉉,李定均 서울醫大 1990 精神醫學 Vol.15 No.1

일본적 생산시스템의 구조와 국제적 논쟁에 관한 일 고찰

주무현 慶北大學校出版部 1998 經商論集 Vol.26 No.2