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Structure analysis of anti-enterovirus 71 polysaccharide from the root of Sanguisorba officinalis
Minyeong Kim,Jiye Park,Ju Yeup Kim,Yong Jin You,Ye-jin Choi,Hye-Jin Park,Seo-Hyeon Mun,Myounghai Kwak,Hyun-Jeong Ko,Seung-Hoon Baek 한국분석과학회 2021 학술대회논문집 Vol.2021 No.11
Increasing prevalence and pandemic risk of viral diseases urgently need the development of safe and effective treatment. The root of Sanguisorba officinalis (SO) has been traditionally used for infectious diseases. In this study, we aimed to elucidate the structure of polysaccharide isolated from SO that inhibits the infection of enterovirus 71 (EV71) causative agent for hand, foot, and mouth disease. The purified macromolecule (S-a3, 260.4 kDa) was a homogenous polysaccharide consisted of galacturonic acid (26.1%), galacturonic acid-6O-methyl ester (14.1%), glucose (20.2%), arabinose (18.3%), galactose (11.8%), and rhamnose (3.7%) residues. Linkage and NMR analysis exhibited that S-a3 consisted of different types of polysaccharides interlinked together including homogalacturonan, rhamnogalacturonan-I, 1,4-α-glucan, and arabinogalactan. S-a3 significantly inhibited cell death and viral gene expression in the EV71-infected Vero cell, and alleviated EV71-induced body weight loss, death, and paralysis in the hSCARB2 transgenic mice model. Importantly, S-a3 was non-toxic to cells and mice at the effective concentration. The antiviral effect of S-a3 was associated with disturbance of EV71 attachment on host cells. Our findings demonstrate that a polysaccharide of SO can be a safe and effective treatment for prevention of EV71 infection.
대황 감초 복합추출물의 항산화 효과를 통한 역류성 식도염 개선 효과
김민영 ( Minyeong Kim ),신유옥 ( Yuock Shin ),이주영 ( Jooyoung Lee ),이아름 ( Ahreum Lee ),신성호 ( Sungho Shin ),권오준 ( Ojun Kwon ),서부일 ( Buil Seo ),노성수 ( Seong-soo Roh ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.4
Objectives : The present study was designed to evaluate the anti-inflammatory and anti-oxidative stress activities through regulation of Nrf2-mediated genes by Rhei rhizoma and Glycyrrhiza rhizoma combined extract (RGE) in reflux esophagitis. Methods : The antioxidant activity of RGE in vitro was measured in terms of radical scavenging capacity such as DPPH and ABTS. RGE was administered at 350 mg/kg body weight prior to induction of reflux esophagitis. Reflux esophagitis was induced that tied the pylorus and the transitional junction between the forestomach and the corpus in Sprague-Dawley rats. Results : RGE scavenged DPPH and ABTS effectively and IC50 of RGE each were 4.9 μg/ml and 45.6 μg/ml. Our results show that RGE administration markedly ameliorated mucosal damage upon histological evaluation. In serum and esophagus tissue, RGE significantly suppressed the oxidative stress biomarkers. Reflux esophagitis induced rats exhibited down-regulation of antioxidant-related proteins in the esophagus; however, the levels with treatment of RGE were significantly higher than those of vehicle reflux esophagitis rats. RGE treatment caused significant reductions in activation of NF-κB transcription factor. Thus, RGE significantly exhibited potent anti-inflammatory activities by suppressing the protein expression levels of pro-inflammatory proteins such as COX-2 and iNOS and inflammatory cytokines such as TNF-α in the esophagus tissue. Conclusions : Reflux esophagitis caused considerable levels of oxidative stress in the esophageal mucosa and the administration of RGE reduced the esophageal mucosa damage through the regulation of Nrf2 and NF-κB pathways. Our findings can considered as supplementary therapy in the prevention or treatment of reflux esophagitis.
급성 위염 유발 마우스 동물 모델에서 구기자(枸杞子) 에탄올 추출물의 위점막 손상보호 효과
이아름 ( Ahreum Lee ),이주영 ( Jooyoung Lee ),김민영 ( Minyeong Kim ),신미래 ( Mi Rae Shin ),신성호 ( Sungho Shin ),서부일 ( Buil Seo ),권오준 ( Ojun Kwon ),노성수 ( Seong Soo Roh ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.6
Objectives : Gastric lesions affect many people around the world and their development are results of the imbalance between destructive and protective factors in the gastric mucosa. Lycium chinense has been widely used as a traditional Korean medicine, it was recently reported that they have potent anti-inflammatory effects in chronic hepatitis models. Therefore, this study aimed to investigate the anti-inflammatory activity of Lycium chinense extract (LCE) on HCl-Ethanol induced gastric lesion mice. Methods : The ICR mice were divided randomly into five groups of six animals each. Group A was normal mice, and group B was treated orally with 0.5 ml 150 mM HCl-60% Ethanol. Mice in group C and D were pre-treatment of LCE (100 mg/kg and 200 mg/kg bodyweight, p.o before HCl/ethanol treatment) and group E was orally administered sucralfate (10 mg/kg). Results : 150mM HCl/60% ethanol-induced gastric mucosal injury mice were ameliorated mucosal damage upon histological evaluation by treatment of LCE. Pre-treatment of LCE attenuated reactive oxidative species (ROS) and produces peroxynitrite (ONOO-) in stomach tissues. As results of stomach protein analyses, LCE effectively reduce inflammatory-related factors such as cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), inducible nitric oxide synthase (iNOS) and interleukin-6 (IL-6) in gastric lesion mice. In addition, nuclear factor kappa B (NF-κB) and inhibitor of phosphorylation of nuclear factor kappa B (p-IκB) were down-regulated in LCE-administrated gastric lesion mice. Conclusions : Our discovery supports that the therapeutic activity of LCE ameliorate the development of gastric lesion via suppressing the oxidative stress and gastric partial inflammation induced by 150 mM HCl/60% ethanol.