Timing and tectonic settings of the Late Paleozoic intrusions in the Zhusileng, northern Alxa: implication for the metallogeny

Mingming Fei,Mao Pan,Chunlin Xie,Jianhui Wang,Hansen Zhao 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.1

The Zhusileng–Hangwula tectonic zone, located in the northern Alxa area, is a key site for understanding the evolution of the Paleo-Asian Ocean (PAO) and related mineral formation. We report the major and trace elements and zircon U-Pb ages of newly discovered mafic and intermediate to felsic intrusive rocks in the Zhusileng area. LA-ICP-MS zircon U-Pb analyses of the Echenghei diabase and Haierhan and Heipingshan granites yielded crystallization ages of 305 Ma, 284 Ma, and 271 Ma, respectively. The Late Carboniferous mafic rocks have high MgO and Fe2O3T contents and low K2O and TiO2 contents, and are characterized by depletion of HREEs and notably negative Nb-Ta anomalies, with enrichment of Rb, Ba, and U, which indicate an enriched mantle source. The Early Permian granites of the Zhusileng area are highly differentiated I-type granitoids sourced from mixing of mantle and crust, are high-K calc-alkaline and enriched in LREEs, and exhibit moderately to strongly negative Eu anomalies. All the intrusive rocks formed in a post-collisional extensional environment, which indicates that the branch of the PAO in the northern Alxa area had closed before the Early Permian. The Cu-Mo/Au mineralization in the Zhusileng area formed in the Middle to Late Permian and is genetically linked to the Late Paleozoic post-collisional rifting magmatism from after the closure of the Paleo-Asian Ocean.

Functional significance of cholesterol metabolism in cancer: from threat to treatment

Xiao Mingming,Xu Jin,Wang Wei,Zhang Bo,Liu Jiang,Li Jialin,Xu Hang,Zhao Yingjun,Yu Xianjun,Shi Si 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Cholesterol is an essential structural component of membranes that contributes to membrane integrity and fluidity. Cholesterol homeostasis plays a critical role in the maintenance of cellular activities. Recently, increasing evidence has indicated that cholesterol is a major determinant by modulating cell signaling events governing the hallmarks of cancer. Numerous studies have shown the functional significance of cholesterol metabolism in tumorigenesis, cancer progression and metastasis through its regulatory effects on the immune response, ferroptosis, autophagy, cell stemness, and the DNA damage response. Here, we summarize recent literature describing cholesterol metabolism in cancer cells, including the cholesterol metabolism pathways and the mutual regulatory mechanisms involved in cancer progression and cholesterol metabolism. We also discuss various drugs targeting cholesterol metabolism to suggest new strategies for cancer treatment.

Performance of Homologous and Heterologous Prime-Boost Immunization Regimens of Recombinant Adenovirus and Modified Vaccinia Virus Ankara Expressing an Ag85B-TB10.4 Fusion Protein against Mycobacterium tuberculosis

( Yiming Kou ),( Mingming Wan ),( Wei Shi ),( Jie Liu ),( Zhilei Zhao ),( Yongqing Xu ),( Wei Wei ),( Bo Sun ),( Feng Gao ),( Linjun Cai ),( Chunlai Jiang ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.6

Tuberculosis (TB) remains a serious health issue around the word. Adenovirus (Ad)-based vaccine and modified vaccinia virus Ankara (MVA)-based vaccine have emerged as two of the most promising immunization candidates over the past few years. However, the performance of the homologous and heterologous prime-boost immunization regimens of these two viral vector-based vaccines remains unclear. In the present study, we constructed recombinant Ad and MVA expressing an Ag85B-TB10.4 fusion protein (AdH4 and MVAH4) and evaluated the impact of their different immunization regimens on the humoral and cellular immune responses. We found that the viral vector-based vaccines could generate significantly higher levels of antigen-specific antibodies, IFN-γ-producing splenocytes, CD69⁺CD8⁺ T cells, and IFN-γ secretion when compared with bacillus Calmette-Guerin (BCG) in a mouse model. AdH4-containing immunization regimens (AdH4-AdH4, AdH4-MVAH4, and MVAH4-AdH4) induced significantly stronger antibody responses, much more IFN-γ-producing splenocytes and CD69⁺CD8⁺ T cells, and higher levels of IFN-γ secretion when compared with the MVAH4-MVAH4 immunization regimen. The number of IFN-γ-producing splenocytes sensitive to CD8⁺ T-cell restricted peptides of Ag85B (9-1p and 9-2p) and Th1-related cytokines (IFN-γ and TNF-α) in the AdH4-MVAH4 heterologous prime-boost regimen immunization group was significantly higher than that in the other viral vector-based vaccine- and BCG-immunized groups, respectively. These results indicate that an immunization regimen involving AdH4 may have a higher capacity to induce humoral and cellular immune responses against TB in mice than that by regimens containing BCG or MVAH4 alone, and the AdH4-MVAH4 prime-boost regimen may generate an ideal protective effect.

Wind resistance performance of a continuous welding stainless steel roof under static ultimate wind loading with testing and simulation methods

DaYang Wang,Zhendong Zhao,Tong Ou,Zhiyong Xin,Mingming Wang,Yongshan Zhang 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.32 No.1

Ultrapure ferritic stainless steel provides a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during wind excitation. This study focuses on the wind-resistant capacity of a new continuous welding stainless steel roof (CWSSR) system. Full-scale testing on the welding joints and the CWSSR system is performed under uniaxial tension and static ultimate wind uplift loadings, respectively. A finite element model is developed with mesh refinement optimization and is further validated with the testing results, which provides a reliable way of investigating the parameter effect on the wind-induced structural responses, namely, the width and thickness of the roof sheeting and welding height. Research results show that the CWSSR system has predominant wind-resistant performance and can bear an ultimate wind uplift loading of 10.4 kPa without observable failures. The welding joints achieve equivalent mechanical behaviors as those of base material is produced with the current of 65 A. Independent structural responses can be found for the roof sheeting of the CWSSR system, and the maximum displacement appears at the middle of the roof sheeting, while the maximum stress appears at the connection supports between the roof sheeting with a significant stress concentration effect. The responses of the CWSSR system are greatly influenced by the width and thickness of the roof sheeting but are less influenced by the welding height. Ultrapure ferritic stainless steel provides a new generation of long-span metal roof systems with continuous welding technology, which exhibits many unknown behaviors during wind excitation. This study focuses on the wind-resistant capacity of a new continuous welding stainless steel roof (CWSSR) system. Full-scale testing on the welding joints and the CWSSR system is performed under uniaxial tension and static ultimate wind uplift loadings, respectively. A finite element model is developed with mesh refinement optimization and is further validated with the testing results, which provides a reliable way of investigating the parameter effect on the wind-induced structural responses, namely, the width and thickness of the roof sheeting and welding height. Research results show that the CWSSR system has predominant wind-resistant performance and can bear an ultimate wind uplift loading of 10.4 kPa without observable failures. The welding joints achieve equivalent mechanical behaviors as those of base material is produced with the current of 65 A. Independent structural responses can be found for the roof sheeting of the CWSSR system, and the maximum displacement appears at the middle of the roof sheeting, while the maximum stress appears at the connection supports between the roof sheeting with a significant stress concentration effect. The responses of the CWSSR system are greatly influenced by the width and thickness of the roof sheeting but are less influenced by the welding height

An Optimized Method of Translating SQL to More Efficient Map-reduce Tasks

Jin Cao,Honglin Han,Mingming Zhao,Sijing Ye,Dehai Zhu,Lin Li 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.4

Phosphoinositide‑specific phospholipase C gene involved in heat and drought tolerance in wheat (Triticum aestivum L.)

Wang Xianguo,Yao Xiaolu,Zhao Ahui,Yang Mingming,Zhao Wanchun,LeTourneau Melissa K.,Dong Jian,Gao Xiang 한국유전학회 2021 Genes & Genomics Vol.43 No.10

Background Phosphoinositide-specifc phospholipase C proteins mediate environmental stress responses in many plants. However, the potential of PI-PLC genes involved with abiotic stress tolerance in wheat remains un-explored. Objective To study TaPLC1 genetic relation with wheat drought and heat resistance. Methods The seedlings were treated with PI-PLC inhibitor U73122 at the single leaf stage. The seedlings were treated with drought and heat stress at the two leaf stage, and some physiological indexes and the expression profle of TaPLC1 gene were determined. And the TaPLC1 overexpression vector was transferred to Arabidopsis and selected to T3 generation for drought and heat stress treatment. Results After 4 h of drought and heat stress, the SOD activity, MDA and soluble sugar content of the two cultivars with inhibitor were higher than those without inhibitor, the chlorophyll content decreased. CS seedlings showed signifcant wilting phenomenon, and TAM107 showed slight wilting. After the elimination of drought and heat stress, all seedling wilting gradually recovered, while the leaf tips of the two varieties treated with inhibitors began to wilt and turn yellow, which was more signifcant 5 days after the drought and heat stress, while the degree of spring wilting and yellow in CS was earlier than that in TAM107. The expression patterns of TaPLC1 gene were diferent in the two cultivars, but the expression levels reached the maximum at 30 min of heat stress. The change of TaPLC1 expression in TAM107 without inhibitor treatment was signifcantly greater than that in CS. The expression level of TaPLC1 in the two cultivars under stress was signifcantly diferent between the two cultivars treated with inhibitor and untreated, and was lower than that of the normal plants under normal conditions. These results indicated that inhibition of TaPLC1 gene expression could enhance the sensitivity of seedlings to stress. In Arabidopsis, the root lengths of transgenic and wild-type seedlings were shortened after drought stress treatment, but the root lengths of transgenic plants decreased slightly. And the expression of TaPLC1 gene was signifcantly increased after drought and heat stress. This indicated that overexpression of TaPLC1 improved drought resistance of Arabidopsis. Conclusions The results of this study suggest that TaPLC1 may be involved in the regulation mechanism of drought and heat stress in wheat.

MODEL-BASED CONTROL OF SYNCHRONIZER SHIFTING PROCESS FOR TRAJECTORY TRACKING CONTROL

Yan Zhang,Han Zhao,Mingming Qiu,Feifei Qin,Bingzhan Zhang 한국자동차공학회 2020 International journal of automotive technology Vol.21 No.4

A novel approach of the tracking control for synchronizer displacement is introduced in this paper. Based on modeling for the structure of synchronizer and shifting process, a shifting displacement tracking controller is designed by the Udwadia - Kalaba equation. The engagement rule of synchronizer combination sleeve is regarded as the trajectory constraint of the system, certain constraint force is imposed to follow this trajectory constraint, which could be obtained by the Udwadia- Kalaba equation without using Lagrange multiplier or other auxiliary variables. Specific comparative study with conventional PID control is discussed. Simulations and vehicle test results show that the shifting actuator can accurately track the desired trajectory determined by the upper layer control strategy, thus verify the effectiveness of the controller.

현장학교에서의 뇌 과학 연구 활용의 장애요인분석 및 대안탐색

강대용,양찬주,문하은,추가람,ZHAO MINGMING 한국교육학회 2017 한국교육학회 학술대회 Vol.2017 No.-

Complete genome sequence and pathogenic genes analysis of Pectobacterium atroseptica JG10-08

Dai Zhang,Yuan Zhou,Dongmei Zhao,Zhihui Yang,Mingming Zhu,Jiehua Zhu 한국유전학회 2017 Genes & Genomics Vol.39 No.9

Pectobacterium atroseptica is known as a rod-shaped gram-negative bacterial pathogen associated with the blackleg of potato. P. atroseptica has been widely identified as the predominant agent causing tuber rot in temperate regions, a disease that leads to severe economic losses to potato industry. In this study, we provide the complete genome sequence of P. atroseptica JG10-08, which revealed that P. atroseptica strain JG10-08 carries a single 5,004,926 bp chromosome with 51.15% G+C content and harbors 4252 predicted coding genes. Phylogenetic analysis based on the genome sequences showed a close evolutionary relationship between P. atroseptica and Pectobacterium wasabiae. We discovered total 168 genes were potentially related to pathogenesis including 9 strainspecific genes encoding toxins on the genome of JG10-08. Further comparison with other species in Pectobacterium revealed a better understanding of pathogenic factors, especially secretion systems in P. atroseptica JG10-08. Collectively, the results of this research provide a solid foundation for discovering the underlying pathogenic mechanisms of P. atroseptica and offer the information to develop more effective strategies against blackleg of potatoes.

Comparative genomics analysis of Pediococcus acidilactici species

Li Zhenzhen,Song Qi,Wang Mingming,Ren Junli,Liu Songling,Zhao Shancen 한국미생물학회 2021 The journal of microbiology Vol.59 No.6

Pediococcus acidilactici is a reliable bacteriocin producer and a promising probiotic species with wide application in the food and health industry. However, the underlying genetic features of this species have not been analyzed. In this study, we performed a comprehensive comparative genomic analysis of 41 P. acidilactici strains from various ecological niches. The bacteriocin production of 41 strains were predicted and three kinds of bacteriocin encoding genes were identified in 11 P. acidilactici strains, namely pediocin PA-1, enterolysin A, and colicin-B. Moreover, whole-genome analysis showed a high genetic diversity within the population, mainly related to a large proportion of variable genomes, mobile elements, and hypothetical genes obtained through horizontal gene transfer. In addition, comparative genomics also facilitated the genetic explanation of the adaptation for host environment, which specify the protection mechanism against the invasion of foreign DNA (i.e. CRISPR/Cas locus), as well as carbohydrate fermentation. The 41 strains of P. acidilactici can metabolize a variety of carbon sources, which enhances the adaptability of this species and survival in different environments. This study evaluated the antibacterial ability, genome evolution, and ecological flexibility of P. acidilactici from the perspective of genetics and provides strong supporting evidence for its industrial development and application.