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- 저자 : Honglin Han (검색결과 4 건)
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Honglin Xu,Haifeng Miao,Guanghong Chen,Guoyong Zhang,Yue Hua,Yuting Wu,Tong Xu,Changlei Hu,Mingjie Pang,Leyi Tan,Xin Han,Bin Liu,Yingchun Zhou 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6
Background: Myocardial fibrosis post-myocardial infarction (MI) can induce maladaptive cardiacremodeling as well as heart failure. Although 20(S)-ginsenoside Rg3 (Rg3) has been applied to cardiovasculardiseases, its efficacy and specific molecular mechanism in myocardial fibrosis are largely unknown. Herein, we aimed to explore whether TGFBR1 signaling was involved in Rg3's anti-fibrotic effectpost-MI. Methods: Left anterior descending (LAD) coronary artery ligation-induced MI mice and TGF-b1-stimulated primary cardiac fibroblasts (CFs) were adopted. Echocardiography, hematoxlin-eosin andMasson staining, Western-blot and immunohistochemistry, CCK8 and Edu were used to study the effectsof Rg3 on myocardial fibrosis and TGFBR1 signaling. The combination mechanism of Rg3 and TGFBR1 wasexplored by surface plasmon resonance imaging (SPRi). Moreover, myocardial Tgfbr1-deficient mice andTGFBR1 adenovirus were adopted to confirm the pharmacological mechanism of Rg3. Results: In vivo experiments, Rg3 ameliorated myocardial fibrosis and hypertrophy and enhanced cardiacfunction. Rg3-TGFBR1 had the 1.78 10 7 M equilibrium dissociation constant based on SPRi analysis,and Rg3 inhibited the activation of TGFBR1/Smads signaling dose-dependently. Cardiac-specific Tgfbr1knockdown abolished Rg3's protection against myocardial fibrosis post-MI. In addition, Rg3 downregulatedthe TGF-b1-mediated CFs growth together with collagen production in vitro through TGFBR1signaling. Moreover, TGFBR1 adenovirus partially blocked the inhibitory effect of Rg3. Conclusion: Rg3 improves myocardial fibrosis and cardiac function through suppressing CFs proliferationalong with collagen deposition by inactivation of TGFBR1 pathway.
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Changes in distribution and morphology of Tamarix ramosissima nebkhas in an oasis-desert ecotone
Chaofan Li,Fan Yang,Xinqian Zheng,Zhangyong Han,Honglin Pan,Chenglong Zhou,Chunrong Ji 한국지질과학협의회 2021 Geosciences Journal Vol.25 No.5
In the oasis-desert ecotone, nebkhas are of great importance for blocking the intrusion of shifting sand and maintaining the ecological security of oases. The morphological characteristics of nebkhas have been widely researched. However, their spatial distribution and morphological variation with the environment remain unclear. In this paper, we systematically analyzed the spatial distribution pattern, morphological changes, and potential indicative significance of Tamarix ramosissima nebkhas (that is, nebkhas formed around T. ramosissima) in a typical oasis-desert ecotone in Northwest China. Our results showed that the intensity of aeolian activities increased from the inside to the outside of the ecotone, and only T. ramosissima shrubs with high tolerance to aeolian activities could survive. Moreover, from the inside to the outside of the ecotone, the density of the T. ramosissima nebkhas decreased, their size increased, and spatial distribution shifted from aggregation to random. The T. ramosissima shrub can trap aeolian transport and protect nebkhas, leading to sand accumulation in the space between, and leeward edges of, the shrubs. Wind erosion mainly occurs to the windward side and along the sides of the nebkhas. Further, the protective effect of the shrub gradually increased with its growth in a year, resulting in the gradually changed of nebkha surface from wind erosion to sand accumulation, and the nebkha volume showed a fluctuating state of first decreasing and then increasing. This indicates that wind erosion and sand accumulation on the nebkha surface were closely related to the growth cycle of the shrub. In addition, nebkhas exhibited a spatial pattern of growth, inside of the ecotone, to degradation, outside of the ecotone. These results indicate that we should focus on the outside of the ecotone to ensure the ecological stability of oases.
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Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Bo-jiang Li,Ping-hua Li,Rui=hua Huang,Wen-xing Sun,Han Wang,Qi-fa Li,Jie Chen,Wang Jun Wu,Honglin Liu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, which is located between the basal lamina and sarcolemma of muscle fibers and is the primary source of myogenic precursor cells in postnatal muscle. Muscle satellite cells are a useful model to investigate the mechanisms of muscle growth and development. Although the isolation and culture protocols of muscle satellite cells in some species (e.g. mouse) have been established successfully, the culture system for porcine muscle satellite cells is very limited. In this study, we optimized the isolation procedure of porcine muscle satellite cells and elaborated the isolation and culture process in detail. Furthermore, we characterized the porcine muscle satellite cells using the immunofluorecence. Our study provides a reference for the isolation of porcine muscle satellite cells and will be useful for studying the molecular mechanisms in these cells.
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