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서민구 ( Min Goo Seo ),도재철 ( Jae Cheul Do ),우인옥 ( In Ohk Ouh ),조민희 ( Min Hee Cho ),김중규 ( Joong Kew Kim ),김영환 ( Young Hoan Kim ),박노찬 ( No Chan Park ),곽동미 ( Dong Mi Kwak ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.4
Prevalence of infectious agents, including Brucella abortus (BA), Mycobacterium bovis (MB), bovine leukemia virus (BLV), M. avium subsp. paratuberculosis (MP), Neospora caninum (NC) and Toxoplasma gondii (TG), was investigated in all the cattle raised in Ulleung island during 2007∼ 2010. For BA, the prevalences in head and farm were 8.1% (44/545) and 5.5% (4/73) in 2007, all negative in 2008∼2009, and 0.5% (4/774) and 1.7% (1/58) in 2010, respectively. For MB, no sample was positive by PPD or ELISA in 2007∼2010. For BLV and MP, no sample was positive by ELISA in 2007∼2009. For NC, seroprevalences in head and farm were 0.2% (1/545) and 1.4% (1/73), respectively, in 2007 and all negative in 2008∼2009. For TG, seroprevalences in head and farm were 17.6% (97/552) and 54.8% (34/62) by ELISA in 2009. By regions, the seroprevalences of TG in Ulleung-eup, Seo-myeon and Buk-myeon were 26.0%, 9.8% and 16.7%, respectively, which had significant differences (P<0.0001). Tiger cattle were more resistant to TG infection than Hanwoo. The seroprevalence of TG in summer was higher than in autumn. The seroprevalence of TG in cows was higher than in oxen. The seroprevalence of TG in cattle was increased with age. In conclusion, this study indicates that the prevalences of six infectious diseases, except for TG which are widely spread, are relatively low in cattle reared in Ulleung island.
DEMS(Diethoxymethylsilane) precursor를 이용한 PECVD 저유전물질 박막증착연구
강민구,김대희,김영철,서화일,Kang, Min-Goo,Kim, Dae-Hee,Kim, Yeong-Cheol,Seo, Hwa-Il 한국반도체디스플레이기술학회 2008 반도체디스플레이기술학회지 Vol.7 No.4
We studied deposition of low-k SiOCH dielectric film by PECVD. DEMS(diethoxymethlysilane) precursor, which has two ethoxy groups along with one methyl group attached to the silicon atoms, was used as precursor. The SiOCH film was deposited as a function of oxygen flow rates ranging from 0 to 100sccm. The deposition rate($\AA$/min) of SiOCH film was increased due to the increase of oxygen radical as a function of $O_2$ flow rates. The dielectric constant was decreased from 3.0 to 2.77, as the film was annealed at $450^{\circ}C$ for 30 min. So, it could account that the dielectric constant changes sensitively with $O_2$ flow rates. Also, the leakage current of the annealed film exhibited stable curve than that of asdeposited. These results were caused by the increase of Si-O-Si group and decrease of Si-CH group and OH group within the film by annealing.
Facile Synthesis and Radioiodine Labeling of Hypericin
Kim, Sang-Wook,Park, Jeong-Hoon,Yang, Seung-Dae,Hur, Min-Goo,Kim, Yu-Seok,Chai, Jong-Seo,Kim, Young-Soon,Yu, Kook-Hyun Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.8
Hypericin (1,3,4,6,8,13-hexahydroxy-10,11-dimethylphenanthro[1,10,9,8-opqra]perylene-7,14-dione), an antidepressant which is also known to be a potent protein kinase C (PKC) inhibitor was synthesized as a precursor for radioiodine labeling via two step reactions. Malignant glioma cells express higher PKC activity compared to untransformed glial cell. Here we report the synthesis and radioiodine labeling of hypericin as a potential brain tumor imaging radiopharmaceutical. The reference compound, 2-iodohypericin, and its radiolabelled analogues, 2-[$^{123}I$]iodohypericin and 2-[$^{124}I$]iodohypericin have been prepared by the reaction of hypericin with NaI or [$^{123}I$]NaI or [$^{124}I$]NaI. The labeling yield was 60-65% for each analogue and the optimal reaction time was 10 min. The purification and isolation of the labelled products were achieved by a reversed-phase HPLC.
Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column
Min-Goo Seo,Tae-Hwan Kim,Oh-Deog Kwon,Kyu-Shik Jeong,Young Ju Lee,Seung-Joon Kim,Sang-Joon Park,Young Sam Kwon,Dong-Mi Kwak 한국실험동물학회 2010 Laboratory Animal Research Vol.26 No.2
Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
Min-Goo Seo,In-Ouh Ouh,Seung-Hun Lee,Ui-Han Son,Paul John L. Geraldino,Man Hee Rhee,Oh-Deog Kwon,Tae-Hwan Kim,Dongmi Kwak 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.3
Anaplasmosis is a tick-borne, non-contagious, zoonotic disease caused by Anaplasma spp., which include Anaplasma marginale, A. centrale, A. phagocytophilum, A. platys, A. ovis, and A. bovis. Recently, in Korea, the prevalence of Anaplasma spp. has been investigated in some animals, such as dogs, horses, goats, cats, and Korean water deer. In cattle, A. marginale is the most virulent species and regarded as the typical type of species. However, data on the seroprevalence of Anaplasma spp. in cattle in Korea during the last decade is limited. This study was designed to investigate the seroprevalence of bovine anaplasmosis in Korea. From 2010 to 2013, blood samples were collected from 568 cattle. Forty animals (7.0%) tested seropositive for Anaplasma spp. by cELISA. Despite that current bovine anaplasmosis seropositivity rate in the Gyeongsangbuk-do is lower than those in tropical countries, anaplasmosis needs to be regarded as a concerning disease. The identification of the specific Anaplasma species infecting cattle in this province requires additional molecular studies. Moreover, further monitoring and control programs for bovine anaplasmosis is required, and the information from this study will be beneficial to develop these programs.
Seo, Min-Goo,Ouh, In-Ohk,Kwon, Oh-Deog,Kwak, Dongmi Elsevier 2018 Molecular phylogenetics and evolution Vol.126 No.-
<P><B>Abstract</B></P> <P> <I>Anaplasma phagocytophilum</I> is the causative agent of human granulocytic anaplasmosis and tick-borne fever in domestic ruminants. Differential diagnosis of zoonotic and pathogenic tick-borne diseases like granulocytic anaplasmosis is important for the efficient implementation of control programs. Thus, the differentiation of pathogenic <I>A. phagocytophilum</I> from non-pathogenic <I>A. phagocytophilum</I>-like (APL) <I>Anaplasma</I> spp. is essential. Recent molecular analyses of APL revealed its distinct phylogenetic position from <I>A. phagocytophilum</I>. This study was conducted to detect <I>A. phagocytophilum</I> and genetically related strains in 764 cattle in South Korea using PCR and restriction fragment length polymorphism assays. APL clade A and <I>A. phagocytophilum</I> were identified in 20 (2.6%) and 16 (2.1%) cattle, respectively, with 16 cattle (2.1%) displaying co-infection. The 16S rRNA sequences of APL clade A were similar (98.3–99.9%) to those clustered in the APL clade A from eastern Asia. The <I>A. phagocytophilum</I> 16S rRNA sequence shared 98.6–100% identity to those of the <I>A. phagocytophilum</I> group. We used PCR to amplify the <I>groEL</I> and <I>msp2</I> genes from the 20 samples positive for the 16S rRNA gene and found that 16 were positive for the <I>groEL</I> sequences in the APL clade A, which showed identity (82.8–84.4%) to those clustered in the APL clade A from Japan. Amplification of <I>msp2</I> was unsuccessful. The co-infection results suggested sequence diversity in <I>Anaplasma</I> spp. Till date, both <I>A. phagocytophilum</I> and APL have been reported to be distributed separately in several animals throughout South Korea. This report is the first co-detection of <I>A. phagocytophilum</I> and APL in Korean cattle using molecular methods. Further studies are needed to provide additional molecular background and trace the evolutionary tree of <I>Anaplasma</I> species in animals and ticks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genotyping of <I>A. phagocytophilum</I> (AP) and <I>A. phagocytophilum</I>-like (APL) strains. </LI> <LI> Sixteen AP and 20 APL were detected in 764 cattle by 16S rRNA sequencing and RFLP assay. </LI> <LI> The APL strains were more divergent in the <I>groEL</I> gene than in the 16S rRNA gene. </LI> <LI> Amplification of the <I>groEL</I> and <I>msp2</I> gene was unsuccessful in AP. </LI> <LI> First detection of AP and APL in Korean cattle and possible circulation of new APL. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Min-Goo Seo,Oh Deog Kwon,곽동미 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
In a population-based study with 4 years of follow up, we evaluated the prevalence of Coxiella burnetii in cattle on Ulleung Island, Korea. In this study, the rates of C. burnetii infection in cattle on Ulleung Island were determined by PCR and were found to be 0.3-1.0% in the period 2011-2014. All 17 C. burnetii partial 16S rRNA gene sequences from PCR-positive cattle were identical and 2 geographic representatives were included in our analysis. The nucleotide se- quences of the 2 samples showed high (98.4-100%) identity with C. burnetii sequences obtained from the GenBank. In this long-term tracking study, the number of cattle positive for C. burnetii on Ulleung Island was low. To prevent the trans- mission of C. burnetii on Ulleung Island, control strategy should include biosecurity improvement in surveillance, livestock management, administering suitable tests before purchasing animals to detect C. burnetii shedders, and restricting movements between herds.