Function of Rhs proteins in porcine extraintestinal pathogenic Escherichia coli PCN033

Lu Wenjia,Tan Jia,Lu Hao,Wang Gaoyan,Dong Wenqi,Wang Chenchen,Li Xiaodan,Tan Chen 한국미생물학회 2021 The journal of microbiology Vol.59 No.9

Extraintestinal pathogenic Escherichia coli (ExPEC) is an important zoonotic pathogen that places severe burdens on public health and animal husbandry. There are many pathogenic factors in E. coli. The type VI secretion system (T6SS) is a nano-microbial weapon that can assemble quickly and inject toxic effectors into recipient cells when danger is encountered. T6SSs are encoded in the genomes of approximately 25% of sequenced Gram-negative bacteria. When these bacteria come into contact with eukaryotic cells or prokaryotic microbes, the T6SS assembles and secretes associated effectors. In the porcine ExPEC strain PCN033, we identified four classic rearrangement hotspot (Rhs) genes. We determined the functions of the four Rhs proteins through mutant construction and protein expression. Animal infection experiments showed that the Δrhs-1CT, Δrhs-2CT, Δrhs-3CT, and Δrhs-4CT caused a significant decrease in the multiplication ability of PCN033 in vivo. Cell infection experiments showed that the Rhs protein is involved in anti-phagocytosis activities and bacterial adhesion and invasion abilities. The results of this study demonstrated that rhs1, rhs3, and rh4 plays an important role in the interaction between PCN033 and host cell. Rhs2 has contribution to cell and mice infection. This study helps to elucidate the pathogenic mechanism governing PCN033 and may help to establish a foundation for further research seeking to identify potential T6SS effectors.

Neonatal Immune State Is Influenced by Maternal Allergic Rhinitis and Associated With Regulatory T cells

Lu Tan,Jing Ou,Zezhang Tao,Yonggang Kong,Yuxuan Zhang 대한천식알레르기학회 2017 Allergy, Asthma & Immunology Research Vol.9 No.2

Purpose: Maternal influences contribute to the origin of allergic diseases, but the mechanisms are not clear. The current literature prompted the role of epigenetics in the development of allergic diseases. We sought to investigate the roles of regulatory T (Treg) cells and Forkhead box p3 (Foxp3) DNA methylation in the process of maternal transmission of allergic rhinitis (AR) susceptibility. Methods: BALB/c female mice (AR mother) were sensitized by intraperitoneal injection of Dermatophagoides pteronyssinus (Der p) 1 on day 1 and 7. Then they mated with normal male mice on day 8. From day 21 to 28, the female mice were intranasal challenged with Der p 1 continuously. The normal controls were given with normal saline in the same way. On postnatal day 3, Female mice and their offspring were sacrificed to detect their histopathology in nasal mucosae, cytokines in sera of mother and spleen homogenates of offspring, Treg cells count, Foxp3 mRNA expressions, and Foxp3 DNA methylation levels in spleens. Results: Compared with the normal controls, neonatal offspring of Der p 1-stimulated female mice (AR offspring) showed the elevation of interleukin (IL)-4 (P<0.01) and IL-17 (P<0.01), the submission of IL-10 (P<0.01) in spleen homogenates. Further, Treg cells count in AR offspring decreased remarkably compared with the normal offspring (P<0.01). Though the difference of Foxp3 DNA methylation level between AR offspring and normal control offspring was not obvious, correlation analysis demonstrated that there was significantly positive correlation between Foxp3 DNA methylation level of mother and that of offspring (r=0.803, P<0.01). Conclusions: Under the influence of Maternal AR, their neonatal offspring develop into T-helper type 2 (Th2) dominant immune state, which is closely associated with the recession of Treg cells. Foxp3 DNA methylation may be a mechanism responsible for that maternal effect but still need more studies to ensure.

TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis

Tan Lu,Fu Lisheng,Zheng Li,Fan Wenjun,Tan Hanyu,Tao Zezhang,Xu Yu 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.2

Purpose: Previous studies have shown the role of ten-eleven translocation 2 (TET2) in CD4+ T cells. However, its function in CD4+ T cells under allergic inflammation is unclear. We aimed to investigate the epigenomic distribution of DNA 5-hydroxymethylcytosine (5hmC) and the role of TET2 in CD4+ T cells of allergic rhinitis (AR). Methods: The hMeDIP-seq was performed to identify sequences with 5hmC deposition in CD4+ T cells of AR patients. Tet2-deficient or wild type mice were stimulated with ovalbumin (OVA) to develop an AR mouse model. The histopathology in nasal mucosae, Th1/Th2/Treg/Th17 cell percentage, concentrations of Th-related cytokines, expression of Tet and differential hydroxymethylated genes (DhMG), and the global deposition of 5hmC in sorted CD4+ T cells were detected. Results: Epigenome-wide 5hmC landscape and DhMG in the CD4+ T cells of AR patients were identified. Tet2 depletion did not led to spontaneous inflammation. However, under the stimulation of allergen, OVA, loss of Tet2 resulted in the exacerbation of allergic inflammation, which was characterized by severer allergic symptoms, more inflammatory cells infiltrating the nasal lamina propria, sharper imbalances between Th1/Th2 and Treg/Th17 cells, and excessive secretion of OVA-specific IgE and Th2-related cytokines. Moreover, altered mRNA production of several DhMG and sharp decrease in 5hmC deposition were also observed in Tet2-deficient OVA-exposed mice. Conclusions: TET2 may regulate DNA 5hmC, DhMG expressions, and CD4+ T cell balance in AR.

Circ_0026359 induces HOXA9 to regulate gastric cancer malignant progression through miR‑140‑3p

Lu Shuirong,Lu Jinlai,Liu Lang,Sun Yilong,Zhao Yixuan,Tan Xi,Li Jingze 한국응용생명화학회 2022 Applied Biological Chemistry (Appl Biol Chem) Vol.65 No.4

Recent researches indicate the key role of circRNA in gastric cancer (GC) progression. However, the role of circ_0026359 in GC progression remains unclear. This study aims to analyze the role of circ_0026359 in GC development and the underlying mechanism. The results showed that compared with controls, GC tissues and cells displayed high circ_0026359 and HOXA9 expression, and low miR-140-3p expression. Depletion of circ_0026359 repressed cell proliferation, migration, invasion and glycolysis, and induced cell apoptosis. Circ_0026359 knockdown inhibited neoplasm growth in vivo. Under-expression of miR-140-3p, a target miRNA of circ_0026359, relieved the effects of circ_0026359 knockdown on GC progression. Additionally, HOXA9 was regulated by the circ_0026359/miR-140-3p axis. Thus, circ_0026359 absence inhibited GC progression by miR-140-3p/HOXA9 pathway, which provided an effective therapeutic target for GC.

【社會科學】 : 在韓中國留學生心理幸福感的影響要因分析及相關探究 - 以大邱,慶北地區爲例 -

륙탄생 ( Tan Sheng Lu ) 한국외국어대학교 중국연구소 2014 中國硏究 Vol.62 No.-

The goal of this research is to explore the relationship between Loneliness, Ego-resilience and Psychological Well-being of Chinese students in Korea. For this purpose, a survey questionnaire was given to 133 students from four universities located in Gyeongbuk Province and Daegu. The collected data was analyzed through SPSS 21.0 which applied Frequency Analysis, One-way ANOVA, T-test, Correlation Analysis and Multiple Regression Analysis. The results indicate the following findings. First, the Korean language ability and Ego-resilience had a positive influence on Psychological Well-being. Secondly, the educational expenses and Loneliness was negatively correlated with Ego-resilience and Psychological Well-being. Thirdly, Ego-resilience had an indirect effect on the relationship between Loneliness and Psychological Well-being.

Prognosis and Clinicopathology of CXCR4 in Colorectal Cancer Patients: a Meta-analysis

Li, Lu-Ning,Jiang, Kai-Tong,Tan, Peng,Wang, Ai-Hua,Kong, Qing-Yin,Wang, Cui-Yue,Lu, Hua-Rong,Wang, Jing Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9

The chemokine receptor 4 (CXCR4) has been widely used in diagnosis and prognosis of colorectal cancer (CRC). However, there is no current consensus on the impact of CXCR4 on CRC patients. The purpose of this study was to evaluate the prognostic and clinicopathological importance of CXCR4 in CRC patients. Databases, such as PubMed, Cochrane library, CBM and EMBASE updated to 2014 were searched to include eligible articles. We analysed correlations between CXCR4 expression and clinicopathological features and overall survival (OS). A total of 1, 055 CRC patients from twelve studies were included in the study. The pooled odds ratios (ORs) which indicated CXCR4 expression was likely to be associated with TNM stage (OR=0.43, CI=0.34-0.55, P<0.00001), lymph node status (OR=2.23, CI=1.23-4.05, P=0.008) and vascular invasion (OR=2.21, CI=1.11-4.39, P=0.02). Poor overall survival of CRC cancer was found to be significantly related to CXCR4 overexpression (hazard ratio (HR) 1.36 CI=1.17-1.59, P<0.0001), whereas combined ORs revealed that CXCR4 expression had no correlation with gender or differentiation. Based on the published studies, CXCR4 overexpression in patients w ith CRC indicates poor survival outcome and clinicopathological factors.

韓,中國家認同感與多元文化受用性的比較硏究 - 以韓,中高中學生爲例

육담성 ( Tan Sheng Lu ) 고려대학교 중국학연구소 2015 中國學論叢 Vol.49 No.-

The goal of this research is to explore the relationship between Ethnic Identity, Civic Identity, and Multicultural Acceptability of the high school students in South Korea and China. For this purpose, a survey questionnaire was given to 476 students from four high schools located in Jiang-Su Province of China(240 people) and seven high schools located in Daegu-Gyeongbuk Region of Korea(236 people). The collected data was analyzed through SPSS 22.0 which applied factor analysis, frequency analysis, t-test, and multiple regression analysis. The results indicate the following findings. Firstly, civic identity of Chinese and Korean students was higher compared to ethnic identity. In the Korean students case, their ethnic identity tended to be higher. And Chinese students tend to consider civic identity as a important related thing more strongly than Korean students. Secondly, ethnic identity of the Chinese and Korean students was negatively correlated with multicultural acceptability, and civic identity of the Korean students had a positive influence on multicultural acceptability, but civic identity of the Chinese students was didn`t have correlation with it. The findings of the study suggest many prospective research topics to be investigated in the future.

青岛市中学生网络成瘾倾向的影响因素研究

陸潭晟(Lu, Tan Sheng),馬?(Ma, Yan),許昌德(Huh, Chang Deog) 영남대학교 중국연구센터 2015 중국과 중국학 Vol.- No.24

본 연구는 중국 중학생들의 인터넷 중독에 영향을 주는 다양한 요인을 검토하여 중학생들의 올바른 인터넷 사용태도를 확립시키는 데 목적을 둔다. 이를 위하여 본 연구의 조사대상은 중국 청도시에 소재하는 5개 중학교의 각 학년 학생 330명을 대상으로 설문조사를 실시하였으며, 회수된 총 310부 중 부적절한 자료를 제외한 300부를 분석자료로 활용하였다. 또한 분석모형에서 가족친밀도와 학교생활만족도를 독립변수로, 인터넷 기대를 매개변수로, 인터넷 중독을 종속변수로 설정하여 수집된 자료에 대해 빈도분석, t-test, 다변량분석과 다중회귀분석, 그리고 Sobel-test를 실시하였으며, 이를 토대로 한 연구결과는 다음과 같다. 첫째, 조사대상자의 인구사회학 특성 중 성별, 학년 및 성적은 인터넷 중독에 영향을 미쳤으며, 학년이 높을수록, 성적이 나쁠수록, 남학생이 여학생보다 인터넷 중독의 가능성을 더 높게 보였으며 이는 통계적으로 유의미한 것으로 나타났다. 둘째, 조사대상자의 인터넷 사용 특성 중 컴퓨터 설치장소(자기 방), 인터넷 이용 장소(PC방), 인터넷 이용 유형(채팅, 게임) 등은 인터넷 중독에 영향을 주는 것으로 나타났다. 셋째, 조사대상자의 가족친밀도가 인터넷 기대에 음의 영향을 미치는 반면에 학교생활만족도가 인터넷 기대에 영향을 미치지 않는 것을 확인되었다. 넷째, 가족친밀도, 학교생활만족도가 인터넷 중독에 음의 영향을 미치는 반면에 인터넷 기대가 인터넷 중독에 긍정적인 영향을 미치는 것으로 나타났다. 다섯째, 중국 중학생들의 가족친밀도와 인터넷 중독의 관계에서 인터넷 기대가 부분매개 역할을 하는 것을 확인할 수 있었다. 반면에 학교생활만족도와 인터넷 중독의 관계에서 인터넷 기대의 매개효과는 없는 것으로 나타났다. This study tried to understand what variables influence the juvenile internet addiction tendency. For this purpose, a survey questionnaire was distributed to 330 students from five middle schools located in Qingdao City, Shan-dong Province, China and 300 questionnaires were used for data analysis. The study used SPSS 21.0 to execute Frequency Analysis, t-test, MANOVA, Multiple Regression Analysis and Sobeltest, entering the juvenile internet addiction tendency as a dependent variable, family relationship intimacy and school life satisfaction as independent variables, while internet expectancy was entered as a mediating variables. The study found the following results. First, significant differences in the degree of internet addiction tendency were found among the participants depending on gender, grade level, and GPA. Secondly, there was statistically significant difference in the degree of internet addiction tendency depending on the places where the internet is installed and used. Thirdly, the lower the degree of family intimacy was related to the more serious the internet addiction tendency. In addition, the lower the school life satisfaction was closely related to the more serious the internet addiction tendency. Finally, Internet expectancy had an indirect effect on the relationship between family relationship and internet addiction tendency.

Role of a Novel Pyridostigmine Bromide-Phospholipid Nanocomplex in Improving Oral Bioavailability

Qun-you Tan,Jing-qing Zhang,Ni-ni Hu,Guo-dong Liu,Hua-feng Yin,Li Zhang,Hong Wang,Lu-yang Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X1, X2, and X3 were 8, 40oC, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and −25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (Tmax) 2 h vs 2 h, the maximum concentration (Cmax) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC0-∞) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.

Effects of running-based sprint interval training on isokinetic strength in the college soccer players

Tan Qingwen,Jiang Lu,Xu Min,Seung-Soo Beak 한국운동재활학회 2022 한국운동재활학회 학술대회 Vol.2022 No.06