Association of TNF-α-308 and -238 Polymorphisms with Risk of Cervical Cancer: A Meta-analysis

Pan, Feng,Tian, Jing,Ji, Chu-Shu,He, Yi-Fu,Han, Xing-Hua,Wang, Yong,Du, Jian-Ping,Jiang, Feng-Shou,Zhang, Ying,Pan, Yue-Yin,Hu, Bing Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Published data on the associations between tumor necrosis factor-alpha (TNF-${\alpha}$) promoter -308G>A and -238G>A polymorphisms and cervical cancer risk are inconclusive. To derive a more precise estimation of the relationship, a meta-analysis was performed. Data were collected from MEDLINE and PubMed databases. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were calculated in a fixed/random effect model. 13 separate studies including 3294 cases and 3468 controls were involved in the meta-analysis. We found no association between TNF-${\alpha}$-308G>A polymorphism and cervical cancer in overall population. In subgroup analysis, significantly elevated risks were found in Caucasian population (A vs. G: OR = 1.43, 95% CI = 1.00-2.03; AA vs. GG: OR = 2.09, 95% CI = 1.34-3.25; Recessive model: OR = 2.09, 95% CI = 1.35-3.25) and African population (GA vs. GG: OR = 1.53, 95% CI = 1.02-2.30). An association of TNF-${\alpha}$-238G>A polymorphism with cervical cancer was found (A vs. G: OR = 0.61, 95% CI = 0.47-0.78; GA vs. GG: OR = 0.59, 95% CI = 0.45-0.77; Dominant model: OR = 0.59, 95% CI = 0.46-0.77). When stratified by ethnicity, similar association was observed in Caucasian population (A vs. G: OR = 0.62, 95% CI = 0.46-0.84; GA vs. GG: OR = 0.59, 95% CI = 0.43-0.82; Dominant model: OR = 0.60, 95% CI = 0.44-0.83). In summary, this meta-analysis suggests that TNF-${\alpha}$-238A allele significantly decreased the cervical cancer risk, and the TNF-${\alpha}$-308G>A polymorphism is associated with the susceptibility to cervical cancer in Caucasian and African population.

Variants on ESR1 and their Association with Prostate Cancer Risk: A Meta-analysis

Ding, Xiang,Cui, Feng-Mei,Xu, Song-Tao,Pu, Jin-Xian,Huang, Yu-Hua,Zhang, Jiang-Lei,Wei, Xue-Dong,Hou, Jian-Quan,Yan, Chun-Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. Methods: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. Results: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). Conclusion: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.

Preparation, self-assembly and performance modulation of gold nanoparticles decorated ferrocene-containing hybrid block copolymer multifunctional materials

Jian-Guo Zhang,Xue-Yin Zhang,Hua Yu,Yan-Ling Luo,Feng Xu,Ya-Shao Chen 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.65 No.-

Gold nanoparticles decorated stimuli-responsive copolymer hybrids, poly(2-methacryloyloxyethyl ferrocenecarboxylate)-block-poly(N-isopropylacryamide) decorated with gold nanoparticles, were synthesized through two-step successive RAFT and ensuing in-situ reduction. The hybrids could self-assemble into interesting micelle structures from globular, wormlike to rodlike shapes by altering the quality and compositions of solvents and ionic strength, and exhibited multifunctionality including quasi-reversible electrochemical behavior, redox-stress responsiveness and temperature sensitivity. The physicochemical and electrochemical properties were modulated by tailor-making the system compositions and redox reaction. The copolymer hybrids were expected to broaden their applications in nanobiomedicine including targeted drug carriers and magnetic resonance imaging, optical, electrochemical catalyst, optoelectronics and sensors etc.

Clerodane furanoditerpenoids from the stems of Tinospora sinensis

Jun-Sheng Zhang,De-Feng Xu,Yin-Yin Wang,Ren-Fen Ma,Hua Zhang 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.5

One new clerodane-type furanoditerpenoidtinosinoid A ( 1 ) and nine new nor-clerodane analogs tinosinoids B–J ( 2 − 10 ) have been isolated from the stems ofTinospora sinensis . The structures of the new compoundswith absolute confi gurations have been elucidated by spectroscopicmeans, including MS, NMR and ECD techniques,as well as chemical correlation. Compound 1 is a rare sulfurcontainingclerodane diterpenoid incorporating a 2-mercaptoethanolunit via a thioether bond, while compounds 4 / 5and 9 represent two pairs of unusual equilibrium regioisomersthrough an interesting intramolecular transesterifi cation. Our bioassays established that 1 and 8 displayed moderateantiproliferative eff ects against two human tumor celllines, and 9 and 10 showed signifi cant α -glucosidase inhibitoryactivities. A kinetics study revealed that compound 10was a noncompetitive α -glucosidase inhibitor, and its possiblebinding mode to the enzyme was further probed bymolecular docking experiments.

Comparative Serum Proteomic Analysis of Serum Diagnosis Proteins of Colorectal Cancer Based on Magnetic Bead Separation and MALDI-TOF Mass Spectrometry

Deng, Bao-Guo,Yao, Jin-Hua,Liu, Qing-Yin,Feng, Xian-Jun,Liu, Dong,Zhao, Li,Tu, Bin,Yang, Fan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background: At present, the diagnosis of colorectal cancer (CRC) requires a colorectal biopsy which is an invasive procedure. We undertook this pilot study to develop an alternative method and potential new biomarkers for diagnosis, and validated a set of well-integrated tools called ClinProt to investigate the serum peptidome in CRC patients. Methods: Fasting blood samples from 67 patients diagnosed with CRC by histological diagnosis, 55 patients diagnosed with colorectal adenoma by biopsy, and 65 healthy volunteers were collected. Division was into a model construction group and an external validation group randomly. The present work focused on serum proteomic analysis of model construction group by ClinProt Kit combined with mass spectrometry. This approach allowed construction of a peptide pattern able to differentiate the studied populations. An external validation group was used to verify the diagnostic capability of the peptidome pattern blindly. An immunoassay method was used to determine serum CEA of CRC and controls. Results: The results showed 59 differential peptide peaks in CRC, colorectal adenoma and health volunteers. A genetic algorithm was used to set up the classification models. Four of the identified peaks at m/z 797, 810, 4078 and 5343 were used to construct peptidome patterns, achieving an accuracy of 100% (> CEA, P<0.05). Furthermore, the peptidome patterns could differentiate the validation group with high accuracy close to 100%. Conclusions: Our results showed that proteomic analysis of serum with MALDI-TOF MS is a fast and reproducible approach, which may provide a novel approach to screening for CRC.

The long non-coding RNA uc.4 influences cell differentiation through the TGF-beta signaling pathway

Zijie Cheng,Qijun Zhang,Anwen Yin,Mengwen Feng,Hua Li,Hailang Li,Yun Li,Lingmei Qian 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

In a previous study, we screened thousands of long non-coding RNAs (lncRNAs) to assess their potential relationship with congenital heart disease (CHD). In this study, uc.4 attracted our attention because of its high level of evolutionary conservation and its antisense orientation to the CASZ1 gene, which is vital for heart development. We explored the function of uc.4 in cells and in zebrafish, and describe a potential mechanism of action. P19 cells were used to investigate the function of uc.4. We studied the effect of uc.4 overexpression on heart development in zebrafish. The overexpression of uc.4 influenced cell differentiation by inhibiting the TGF-beta signaling pathway and suppressed heart development in zebrafish, resulting in cardiac malformation. Taken together, our findings show that uc.4 is involved in heart development, thus providing a potential therapeutic target for CHD.

Role of a Novel Pyridostigmine Bromide-Phospholipid Nanocomplex in Improving Oral Bioavailability

Qun-you Tan,Jing-qing Zhang,Ni-ni Hu,Guo-dong Liu,Hua-feng Yin,Li Zhang,Hong Wang,Lu-yang Lu 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.3

A novel pyridostigmine bromide (PB)-phospholipid nanocomplex (PBPLC) was prepared to increase the bioavailability of PB. A central composite design approach was employed for process optimization. The physicochemical properties of PBPLC were investigated by means of differential scanning calorimetry, ultraviolet spectroscopy, Fourier transformed infrared spectroscopy and the n-octano/water partition coefficient. The intestinal permeability of PBPLC was observed via a single pass intestinal perfusion in rats. After oral administration of PBPLC, the concentrations of PB at predetermined time points were determined by HPLC, and the pharmacokinetic parameters were computed by DAS 2.1.1 software. Multiple linear regression analysis for process optimization revealed that the optimal PBPLC was obtained when the values of X1, X2, and X3 were 8, 40oC, and 4 mg/mL, respectively. The average particle size and zeta potential of PBPLC with the optimized formulation were 204.60 nm and −25.12 mV, respectively. Non-covalent interactions between PB and phospholipids were found in the PBPLC. The n-octanol/water partition coefficient of PBPLC was substantially increased. PBPLC had better intestinal permeability in comparison with free PB. Mean plasma drug concentration-time curves of PBPLC and free PB after oral administration were both in accordance with the two-compartment open model. The values of pharmacokinetic parameters of PBPLC and free PB were the peak time (Tmax) 2 h vs 2 h, the maximum concentration (Cmax) 22.79 μg/mL vs 6.00 μg/mL, and the value of the area under the concentration vs time curve (AUC0-∞) 7128.21 μg·min/mL vs 1772.36 μg·min/mL, respectively. In conclusion, compared with free PB, PBPLC remarkably improves the oral bioavailability of PB, which is likely due to its higher lipophilicity and permeability.