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Gan, Lu-Jing,Yang, Dan,Shin, Jung-Ah,Kim, Sun-Ju,Hong, Soon-Taek,Lee, JeungHee,Sung, Chang-Keun,Lee, Ki-Teak American Chemical Society 2012 Journal of agricultural and food chemistry Vol.60 No.1
<P>The effects of the purple-fleshed sweet potato extract(PFSPE)on oxidation stabilities of a model oil-in-water emulsion preparedwith enzymatically synthesized fish oil–soybean oil structuredlipid (SL) versus physically blended lipid (PBL) without modificationwere evaluated. The anthocyanins in PFSPE were analyzed and identifiedby HPLC-MS. The fatty acid composition of SL was similar to that ofPBL, except palmitic acid (1.48 in PBL and 9.61% in SL) and linoleicacid (62.47 in PBL and 49.58% in SL). Peonidin 3-caffeoylsophoroside-5-glucoside,peonidin-3-(6′,6′-caffeoylferuloylsophoroside)-5-glucoside,peonidin-dicaffeoylsophoroside-5-glucoside, peonidin 3-(6′,6″-caffeoyl-<I>p</I>-hydroxybenzoylsophoroside)-5-glucoside were identifiedas the major anthocyanin compounds in PFSPE. Different levels (200,500, 1000 ppm) of PFSPE were added into both SL- and PBL-based emulsions,with 200 ppm catechin as comparison. Oxidation was monitored by measuringthe peroxide value and thiobarbituric acid reactive substances. Theantioxidant activity of PFSPE increased with an increased concentration,the concentration of 1000 ppm showed high antioxidant ability similarto that of catechin in both PBL- and SL-based oil-in-water emulsions.It is notable that the SL-based emulsion appeared to have better oxidativestability than the PBL-based emulsion.</P>
Anatomical Adaptations of the Xerophilous Medicinal Plant, Capparis spinosa, to Drought Conditions
Lu Gan,Chunyu Zhang,Yongtai Yin,Zhiwei Lin,Yongwei Huang,Jun Xiang,Chunhua Fu,Maoteng Li 한국원예학회 2013 Horticulture, Environment, and Biotechnology Vol.54 No.2
Capparis spinosa is a plant that grows in dry and arid environments. As far as can be ascertained, no comprehensive studies on how the leaf, stem and root structures adapt to drought conditions have been published to date. In this paper, a study into the anatomical adaptations of the leaf, stem and root of C. spinosa to drought environments was conducted using in vitro cultured seedlings as control. The results showed that C. spinosa could change its leaf, stem, and root structures when adapting to drought conditions. The plant growing under drought conditions possessed an enlarged transit region between the stem and root where the xylem and fibro-vascular system had increased in order to enhance water absorption and storage capacity. The leaf, stem and root of C. spinosa under drought conditions were better developed than those under normal in vitro culture conditions. The leaf possessed uniform mesophyll cells and three or four layers of palisade mesophyll cells on both sides of the mature leaves. The stomata were evenly distributed across both sides of the leaf, and they remained open continually during the day throughout the summer growing period, especially those on the lower leaf surface. The xylem in the stem was extremely well developed with wide vessels and much thicker cortical layers. All these characteristics can enhance the adaptability of C. spinosa and enable it to survive in extremely dry and arid areas.
Gan, Lu-Jing,Wang, Xiang-Yu,Yang, Dan,Zhang, Hua,Shin, Jung-Ah,Hong, Soon-Taek,Park, Sang Hyun,Lee, Ki-Teak Wiley (John WileySons) 2014 Journal of the American Oil Chemists' Society Vol.91 No.4
Lecitase Ultra and 6 triacylglycerol lipases (lipases PS, M, AH, AY, R, and AK) were immobilized on Amberlite XAD 7HP and used to catalyze the acidolysis reaction between lecithin and capric acid (C10:0) for comparison. The highest molar incorporation value (51.0 mol%) was observed for the immobilized Lecitase Ultra. Further, immobilized Lecitase Ultra was selected for catalyzing acidolysis between lecithin and fatty acids with different chain lengths (C6:0, C8:0, C10:0, C12:0, and C14:0). After reaction, free fatty acids were removed by SPE and the resultant was called modified lecithin fraction 1 (MLF1). The highest molar incorporation value was obtained for C10:0 (51.0 mol%) at 45 A degrees C with a mole ratio of 10/1 (C10:0/lecithin) for 72 h. After removal of lysophosphatidylcholine by solid-phase extraction from MLF1, the resultant modified lecithin fraction 2 (MLF2) was used to prepare an oil-in-water emulsion. All emulsions prepared with MLF2 exhibited significantly higher emulsion stability (ES) values (16.2-17.7) and smaller particle sizes (d (32) 0.40-0.49 mu m, d (43) 0.75-1.01 mu m) than the emulsion prepared with unmodified lecithin (ES 14.1, d (32) 0.76 mu m, d (43), 1.26 mu m) (P < 0.05). Furthermore, less clarification and droplet aggregation were observed in emulsions prepared with MLF2 than in lecithin-based emulsions. Overall, the MLF2s showed better emulsifying properties than lecithin.
Lu Daoyi,Theotokatos Gerasimos,Zhang Jundong,Tang Yuanyuan,Gan Huibing,Liu Qingjiang,Ren Tiebing 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1
This study aims to investigate the impact of the High Pressure Selective Catalytic Reduction system (SCRHP) on a large marine two-stroke engine performance parameters by employing thermodynamic modelling. A coupled model of the zero-dimensional type is extended to incorporate the modelling of the SCR-HP components and the Control Bypass Valve (CBV) block. This model is employed to simulate several scenarios representing the engine operation at both healthy and degraded conditions considering the compressor fouling and the SCR reactor clogging. The derived results are analysed to quantify the impact of the SCR-HP on the investigated engine performance. The SCR system pressure drop and the cylinder bypass valve flow cause an increase of the engine Specific Fuel Oil Consumption (SFOC) in the range 0.3e2.77 g/kWh. The thermal inertia of the SCR-HP is mainly attributed to the SCR reactor, which causes a delayed turbocharger response. These effects are more pronounced at low engine loads. This study supports the better understanding of the operating characteristics of marine two-stroke diesel engines equipped with the SCR-HP and quantification of the impact of the components degradation on the engine performance.
( Gan Xiong ),( Lu Hong ),( Yang Guangyou ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.8
This paper analyzes the nodes deployment optimization problem in energy constrained wireless sensor networks, which multi-hop cooperative beamforming (CB) based cooperative-multi-input-single-output (CMISO) transmission is adopted to reduce the energy consumption. Firstly, we establish the energy consumption models for multi-hop SISO, multi-hop DSTBC based CMISO, multi-hop CB based CMISO transmissions under random nodes deployment. Then, we minimize the energy consumption by searching the optimal nodes deployment for the three transmissions. Furthermore, numerical results present the optimal nodes deployment parameters for the three transmissions. Energy consumption of the three transmissions are compared under optimal nodes deployment, which shows that CB based CMISO transmission consumes less energy than SISO and DSTBC based CMISO transmissions. Meanwhile, under optimal nodes deployment, the superiorities of CB based CMISO transmission over SISO and DSTBC based CMISO transmissions can be more obvious when path-loss-factor becomes low.
Gan Junqing,Liu Shan,Zhang Yu,He Liangzi,Bai Lu,Liao Ran,Zhao Juan,Guo Madi,Jiang Wei,Li Jiade,Li Qi,Mu Guannan,Wu Yangjiazi,Wang Xinling,Zhang Xingli,Zhou Dan,Lv Huimin,Wang Zhengfeng,Zhang Yanqiao,Q 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
The functional role of microRNA-375 (miR-375) in the development of prostate cancer (PCa) remains controversial. Previously, we found that plasma exosomal miR-375 is significantly elevated in castration-resistant PCa (CRPC) patients compared with castration-sensitive PCa patients. Here, we aimed to determine how miR-375 modulates CRPC progression and thereafter to evaluate the therapeutic potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes loaded with miR-375 antisense oligonucleotides (e-375i). We used miRNA in situ hybridization technique to evaluate miR-375 expression in PCa tissues, gain- and loss-of-function experiments to determine miR-375 function, and bioinformatic methods, dual-luciferase reporter assay, qPCR, IHC and western blotting to determine and validate the target as well as the effects of miR-375 at the molecular level. Then, e-375i complexes were assessed for their antagonizing effects against miR-375. We found that the expression of miR-375 was elevated in PCa tissues and cancer exosomes, correlating with the Gleason score. Forced expression of miR-375 enhanced the expression of EMT markers and AR but suppressed apoptosis markers, leading to enhanced proliferation, migration, invasion, and enzalutamide resistance and decreased apoptosis of PCa cells. These effects could be reversed by miR-375 silencing. Mechanistically, miR-375 directly interfered with the expression of phosphatase nonreceptor type 4 (PTPN4), which in turn stabilized phosphorylated STAT3. Application of e-375i could inhibit miR-375, upregulate PTPN4 and downregulate p-STAT3, eventually repressing the growth of PCa. Collectively, we identified a novel miR-375 target, PTPN4, that functions upstream of STAT3, and targeting miR-375 may be an alternative therapeutic for PCa, especially for CRPC with high AR levels.
Lu, Yan-Yan,Huang, Xin-En,Xu, Lin,Liu, De-Gan,Cao, Jie,Wu, Xue-Yan,Liu, Jin,Xiang, Jin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Background: Pemetrexed (PEM) is effective in first-line treatment for patients with non-squamous non-small cell lung cancer (NSCLC). However there are currently no definitive determinants to certify which patients could benefit from PEM. To improve the efficacy of PEM combined with platinum as first-line therapy for advanced non-squamous NSCLC, we conducted this retrospective study to detect potential determinants of this regimen. Methods: We recruited 109 patients with advanced non-squamous NSCLC who received PEM with a platinum as first-line therapy from June 2006 to February 2013 in Jiangsu Cancer Hospital. Multiple variables (age, sex, smoking, degree of cell differentiation, hemoglobin, platinum drugs combined, positions of metastasis) were selected. Logistic regression analysis was used to analyse relationships between these variables and tumor response. Result: In univariate analysis, we found that age and platinum significantly influenced the results of PEM therapy (P<0.05). In multivariable analysis, no factors were independently significant. Conclusion: Our analysis did not suggest that the age, sex, metastasis of liver or other organs, hemoglobin, smoking history and pathological differentiation are associated with the response of PEM. We should conduct further analyses with larger sample size to reconfirm this issue.