Effect of recombinant baculovirus expressing CrV1 protein from Cotesia rubecula bracovirus against Pieris rapae in insecticidal toxicity

Lihua Wei,Miguel A. PEREZ-RODRIGUEZ,Mario A. RODRIGUEZ-PEREZ 한국곤충학회 2016 Entomological Research Vol.46 No.3

Baculoviruses can be genetically engineered to express foreign genes; thus, their lethal potency and host range can be improved to produce more virulent bioinsecticides. Polydnavirus (PDV) genes have insecticidal bioactivities and could enhance the pathogenicity of the baculoviruses to control insect pests. The CrV1 gene from Cotesia rubecula polydnavirus is responsible for depolymerization of actin cytoskeleton in hemocytes, disabling its spread on foreign object surfaces. In this study, we tested the efficacy of the recombinant baculovirus (AcMNPV‐CrV1) under p10 promoter against second instar P. rapae larvae. The expression of the CrV1 gene in P. rapae larvae was verified with reverse transcription–polymerase chain reaction (RT‐PCR). AcMNPV‐CrV1 showed a significantly lower median lethal concentration (LC50) and shorter median lethal time (LT50) as compared with the AcMNPV wild‐type virus. These results suggested that the expression of CrV1 protein could successfully improve the insecticidal toxicity of baculovirus.

Pharmacokinetics, tissue distribution and excretion of peimisine in rats assessed by liquid chromatography-tandem mass spectrometry

Lihua Chen,Dongxun Li,Guosong Zhang,Wei Zhang,Lihua Zhang,Yongmei Guan,Weifeng Zhu,Hongning Liu 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.6

Peimisine, the common ingredient of ‘‘zhebeimu’’groups and ‘‘chuanbeimu’’ groups, is responsiblefor the expectorant and cough relieving effects. The aim ofthis study was to investigate the pharmacokinetics, tissuedistribution and excretion of peimisine in male and femaleSD (Sprague-Dawley) rats by a rapid and sensitive LC-MS/MS (liquid chromatography-tandem mass spectrometry)method used carbamazepine as the internal standard afteroral administration, carbamazepine was stated as an IS. The results showed that peimisine was slowly distributed,and eliminated from rat plasma and manifested lineardynamics in a dose range of 0.26–6.5 mg/kg. Tested byANOVA, there were gender differences in the pharmacokineticparameters of AUC0-t, AUC0-? among a singledose of 0.26, 1.3, 6.5 mg/kg (P\0.05). Drug blood andtissue levels in male rats were significantly higher than thefemale counterparts after oral administration, while boththe males and the females showed high drug levels inspleen, kidney, lung, liver and heart. On the other hand, thepeimisine levels that can be reached in uterus, ovary, testisand brain is low. The excretion study showed that littleadministered peimisine (\0.7 %) was recovered in themale and female bile. Approximately 13.46 and 15.05 %were recovered in female urine and feces, while 43.07 and7.49 % were recovered in male urine and feces, respectively,which indicated that the major elimination route ofmale rats was urine excretion. In addition, there was significantdifferences in total cumulative excretive ratio ofpeimisine in feces (P\0.05) and no significant differencesin the urine (P[0.05) at a dose of 1.3 mg/kg.

Prediction of long-term compressive strength of concrete with admixtures using hybrid swarm-based algorithms

Lihua Huang,Wei Jiang,Yuling Wang,Yirong Zhu,Mansour Afzal 국제구조공학회 2022 Smart Structures and Systems, An International Jou Vol.29 No.3

Concrete is a most utilized material in the construction industry that have main components. The strength of concrete can be improved by adding some admixtures. Evaluating the impact of fly ash (FA) and silica fume (SF) on the longterm compressive strength (CS) of concrete provokes to find the significant parameters in predicting the CS, which could be useful in the practical works and would be extensible in the future analysis. In this study, to evaluate the effective parameters in predicting the CS of concrete containing admixtures in the long-term and present a fitted equation, the multivariate adaptive regression splines (MARS) method has been used, which could find a relationship between independent and dependent variables. Next, for optimizing the output equation, biogeography-based optimization (BBO), particle swarm optimization (PSO), and hybrid PSOBBO methods have been utilized to find the most optimal conclusions. It could be concluded that for CS predictions in the long-term, all proposed models have the coefficient of determination (R²) larger than 0.9243. Furthermore, MARS-PSOBBO could be offered as the best model to predict CS between three hybrid algorithms accurately.

Simulated Long-Term Vegetation–Climate Feedbacks in the Tibetan Plateau

Wei Hua,Zouxin Lin,Donglin Guo,Guangzhou Fan,Yongli Zhang,Kaiqin Yang,Qin Hu,Lihua Zhu 한국기상학회 2019 Asia-Pacific Journal of Atmospheric Sciences Vol.55 No.1

The Tibetan Plateau (TP) is an important region of land–atmosphere interactions with high climate variability. In this study, an atmosphere–vegetation interaction model was applied to explore the possible responses of vegetation to climate warming, and to assess the impacts of land cover change on the land surface physical processes across the TP. Results showed that long-term warming over the TP could influence vegetation growth via different mechanisms. Most likely, increased temperature would enhance the physiological activity in most high cold areas on the TP, whereas high temperature would inhibit vegetation growth by increasing respiration in areas with favorable water and temperature conditions. In addition, for areas where the climate is warmer but not wetter, higher temperature could influence photosynthesis via the moisture condition of the vegetation rather than by modulating respiration. Numerical simulations demonstrated that vegetation could control the land surface–atmosphere energy balance effectively. The change of land cover from vegetated land to desert steppe decreased the net radiation absorbed by the surface, weakening the surface thermal effects, and reducing sensible and latent heat fluxes. Furthermore, sensitivity simulations also revealed that global warming would likely accelerate vegetation growth in most areas of the TP, resulting in increased surface heat flux.

Sodium alginate-based wall materials microencapsulated Lactobacillus plantarum CICC 20022: characteristics and survivability study

Lihua Zhang,Peixin Tang,Shunfeng Li,Xia Wang,Wei Zong 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.11

In this study, microcapsules of Lactobacillus plantarum CICC 20022 (L. plantarum CICC 20022) were prepared by extrusion technique using sodium alginate (SA), sodium alginate-sodium caseinate (SA-SC) and sodium alginate-whey protein isolate (SA-WPI) as wall materials, respectively. Results showed that the best encapsulation yield of L. plantarum CICC 20022 was SA-WPI. Morphology and texture analysis showed that the microcapsules prepared by the SA-WPI system presented a more compact internal structure and higher resistance to external pressure. Fourier-transform infrared spectroscopy and low field nuclear magnetic resonance analysis demonstrated that the hydrogen bonding ability and network structure of the SA were improved by the addition of WPI. The survivability of L. plantarum CICC 20022 entrapped with the SA-WPI system was improved during freeze-drying and simulated gastrointestinal digestion. Therefore, the SA-WPI system can potentially be used as the vector of L. plantarum CICC 20022 in food applications.

Fault Diagnosis for High Order Systems Based on Model Decomposition

Xiukun Wei,Lihua Liu,Limin Jia 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.1

Fault detection observer and fault estimation filter are the main tools for the model based fault diagnosis approach. The dimension of the observer gain normally depends on the system order and the system output dimension. The fault estimation filter traditionally has the same order as the monitored system. For high order systems, these methods have the potential problems such as parameter optimization and the real time implementation on-board for applications. In this paper, the system dynamical model is first decomposed into two subsystems. The first subsystem has a low order which is the same as the fault dimension. The other subsystem is not affected by the fault directly. With the new model structure, a fault detection approach is proposed where only the residual of the first subsystem is designed to be sensitive to the faults. The residual of the second subsystem is totally decoupled from the faults. Moreover, a lower order fault estimation filter (with the same dimension of the fault) design algorithm is investigated. In addition, the design of a static fault estimation matrix is presented for further improving the fault estimation precision. The effectiveness of the proposed method is demonstrated by a simulation example.

A review of cut flower industry in China

Xue Wei Wu,Jeung Keun Suh,Lihua Wang,Dong-Jin Lee 단국대학교 국제농업협력연구소 2012 단국대학교 국제농업협력연구소 학술대회 Vol.2012 No.1

With escalating economic growth during the last three decades, flower industry of China, especially cut flower is sharply developed. In this paper a brief review of the cut flower current situation of globe and current status of flower industry of China especially of cut flower in the world is presented. The acreage, yield, potential of cut flower in China along with distribution of major cut flower products and constraint of cut flower also was indicated in this paper was also presented.

Construction of Eukaryotic Vector Expressing shRNA of Lycopene Cyclase Gene carRA

( Ye Li ),( Lihua Jin ),( Qing Wei ),( Xiaojie Wang ),( Bo Li ),( Yuning Zhang ),( Liqing Zhang ) 조선대학교 공학기술연구원 2012 공학기술논문지 Vol.5 No.2

To construct eukaryotic vector expressing shRNA (short hairpin RNA) of carRA. Methods: Designing three different shRNA targeting the coding sequence of the car RA, the mU6-carRA shRNA was constructed by inserting the designed shRNA to the eukaryotic expression vector mU6pro. The constructed eukaryotic vector expressing shRNA of carRA was transformed into DH5 strain. Finally, the recombinant plasmid identified by restriction endonuclease was used for sequence analysis. Results: It was verified by partial nucleotide sequencing and restriction endonuclease digestion that the constructed eukaryotic vector expressing shRNA of carRA was correct. Conclusion: The results of study lay the foundation for further studying on its inhibitive effect on cycling of lycopene.

Tenascin-C Produced by Oxidized LDL-Stimulated Macrophages Increases Foam Cell Formation through Toll-like Receptor-4

Rui Liu,Lihua Zhang,Yong He,Bo Li,Jun Liu,Yingang Ren,Wei Han,Xing Wang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.1

Atherosclerosis is a chronic inflammatory disease in which both innate and adaptive immunity are involved. Although there have been major advances in the involve-ment of toll-like receptor 4 (TLR4) and CD36 in the initiation and development of this disease, detailed mechanisms remain unknown. Here, we show that tenascin-C (TN-C) can stimulate foam cell formation and this can be inhibited by a TLR4-blocking antibody or CD36 gene silencing. Our results identify TN-C-TLR4 activation as a common molecular mechanism in oxLDL-stimulated foam cell formation and atherosclerosis. In addition, CD36 is the major scavenger receptor responsible for the TN-C-mediated foam cell formation. Taken together, we have identified that TN-C produced by oxLDL-stimulated macrophages increases foam cell formation through TLR4 and scavenger receptor CD36.

The Changes of Photosynthetic Character in Cymbidium faberi Rolfe during the Stages of Recovery after Strong Light Stress

Jun Tao,Lihua Zhu,Ju Yu,Jun Wei 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-