Formulation of nimodipine nanocrystals for oral administration

Jianwen Li,Qiang Fu,Xiaohong Liu,Mo Li,Yongjun Wang 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2

The aim of this paper is to optimize nimodipine (NMD) nanocrystals (NCs) for oral administration. The effects of independent process variables (microprecipitation temperature, shearing speed, shearing time, homogenization pressure and number of cycles) on the particle size have been studied. Experiments were conducted to optimize the formulation composition. A single factor exploration was used to screen the primary stabilizers. Then, the selected polymers/surfactants were further optimized using an L9 (34) orthogonal design. The optimal formulation was composed of NMD (0.7 %, w/v), F127 (0.4 %, w/v), HPMC-E5 (0.1 %, w/v), and sodium deoxycholate (0.05 %, w/v) and was rod-shaped as shown by SEM observations, and it had a particle size of 833.3 ± 20.6 nm, determined by laser diffraction. These aqueous NCs were physically stable for 15 days. To further improve the stability, the NCs were freeze-dried. The powder obtained exhibited acceptable flowability and was physically stable for at least 24 months. Additionally, the NMD NCs displayed much higher dissolution profiles than the bulk drug. The pharmacokinetic results showed that the relative bioavailability was 397 % in comparison with Nimotop, suggesting that NCs are an efficient strategy for improving the oral bioavailability of poorly water-soluble drugs.

Recent development of single preparations and fixed-dose combination tablets for the treatment of non-insulin-dependent diabetes mellitus

Jianwen Li,He Lian 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.6

As a complex endocrine and metabolic disorder,type 2 diabetes mellitus (non-insulin-dependent diabetesmellitus, NIDDM) has become a major threat to humanhealth. Because of the heterogeneous and progressive disordersinduced by insulin resistance and pancreatic b-celldysfunction, the treatment of NIDDM is still challenging. Although antidiabetic drugs with different pharmacologicalmechanisms of action have been used clinically, differentdegrees of undesirable glucose control and the incidencesof a variety of side effects, including hypoglycemia, cardiovascularcomplications and weight gain require thebetter treatment options. This article has overviewed thecurrent literature about commercially available antidiabeticdrugs with different pharmacological mechanisms of actionin the treatment of NIDDM, and summarized the publisheddata regarding the efficacy, tolerability, and safety of currentlyavailable single preparations and fixed-dose combinations,aiming to provide important information for thedevelopment and application of antidiabetic drugs in thefuture. The literature search from 1989 to 2015 wasconducted by PubMed, ScienceDirect, Springer, AmericanDiabetes Association, and U.S. FDA Drugs databases.

Amorphous FePO4/reduced graphene oxide composite prepared from jarosite residue and its application as a novel anode material for lithium-ion batteries

Yanwei Li,Wenhan Xu,Jinhuan Yao,Bin Huang,Shunhua Xiao,Jianwen Yang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-

Rational utilization of jarosite residue is significantly important from the view of environmental protectionand resource recycling. Herein, we report a facile preparation of porous amorphous FePO4/reducedgraphene oxide (rGO) composite by utilizing the iron resource in jarosite residue with a selective chemicalprecipitation method, and demonstrate its great potential as a high-performance anode material oflithium-ion batteries (LIBs). The FePO4/rGO composite exhibits superior cyclic performance(812.7 mA h g1 over 300 cycles at 500 mA g1) and high-rate capability (335.2 mA h g1 even at a highcurrent density of 10 A g1) in a half cell. More importantly, the full LIB assembled with FePO4/rGO anodeand commercial Li(Ni0.5Co0.2Mn0.3)O2 (NCM523) cathode still delivers a high and stable capacity of 674. 9/528.7 mA h g1 upon 100/300 cycles at 200/500 mA g1, indicating the great potential of this FePO4/rGO composite as a new anode material with high lithium storage performance. This work proposes asimple, feasible, and cost-effective strategy for the high-valued utilization of jarosite residue and providesa reference for the design and development of FePO4-based anode materials for LIBs.

Effect of heat treatment on the electrochemical performance of V2O5.nH2O as a cathode material for aqueous rechargeable zinc ion batteries

Jiaqi Li,Yanwei Li,Jinhuan Yao,Bin Huang,Jiqiong Jiang,Jianwen Yang 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.115 No.-

The influence of heat treatment (100 °C, 150 °C, 200 °C, 300 °C, and 400 °C) on the structure and the zinc ion storage performance of V2O5·nH2O is explored. The results show that the interlayer water content, interlayer distance, and V4+ content in V2O5·nH2O can be regulated by heat treatment. Electrochemical characterization demonstrates that the as-prepared vanadium pentoxide without heat treatment shows rather poor cycling stability (only 56 m Ah g−1 after 600 cycles at 0.5 A/g). After being heat treated in the temperature range of 100 to 200 °C, the zinc ion storage performance of vanadium pentoxide can be enhanced dramatically. Particularly, the vanadium pentoxide after being heat-treated at 150 °C exhibits the best cycling stability (179 mA h g−1 after 600 cycles at 0.5 A/g), superior high-rate capability (117 mA h g−1 at 3.0 A/g), and faster electrochemical reaction kinetics. The results reported in this work could provide clues for regulating and optimizing the electrochemical performance of vanadium pentoxide as a cathode material for ZIBs.

Expression of RUNX2/LAPTM5 in the Induction of MC3T3-e1 Mineralization and Its Possible Relationship with Autophagy

Xing Lei,Li Yanqin,Li Wenhao,Liu Rong,Geng Yuanming,Ma Weiqun,Qiao Yu,Li Jianwen,Lv Yingtao,Fang Ying,Xu Pingping 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

BACKGROUND: The study aims to correlate osteogenesis with autophagy during the mineralization induction of MC3T3-e1 through exploring the expression of runt-related transcription factor 2 (RUNX2)/lysosomal-associated transmembrane protein 5 (LAMPT5). METHODS: The induction of mineralization in MC3T3-e1 was followed by detecting the expressions of osteogenesisrelated indexes such as RUNX2, alkaline phosphatase (ALP), osteocalcin (OCN), and LAPTM5 using RT-qPCR and Western blot from 0 to 14 days. Transmission electron microscope was utilised in visualizing the alterations of autophagosomes, which was followed by immunofluorescence detecting the subcellular localization of autophagy-related index sequestosome 1 (P62) and microtubule-associated protein 1 light 3 (LC3) protein and scrutinising the expression of P62 mRNA and P62 and LC3 proteins. RESULTS: Induction of MC3T3-e1 mineralization demonstrated an increased expression of osteogenesis-related indicators such as RUNX2, ALP, OCN, and LAPTM5 (p\0.05), as evident from the results of RT-qPCR and Western blot. Meanwhile, the expression of autophagosomes increased one day after mineralization induction and then experienced a gradual decline, and enhanced expression of LC3 protein was noted on days 1–2 of mineralization induction but was then followed by a corresponding reduce. In contrast, a continuous increase was reported in the expression of P62 mRNA and protein, respectively (p\0.05). Up- and down-regulating RUNX2/LAPTM5 expression alone confirmed the aforementioned results. CONCLUSION: It was therefore proposed that RUNX2 may be responsible for an early increase and then a gradual decrease in LAPTM5-mediated autophagy through the regulation of its high expression. Meanwhile, increased LAPTM5 expression in osteogenic mineralization presumed that RUNX2/LAPTM5 promoted autophagy and osteogenic expression, which may play a bridging role in the regulation of autophagy and osteogenesis.

직접분사식 가솔린 엔진을 위한 충돌확산형 연소에 관한 연구

채재우(J.O.Chae),이상만(S.M.Lee),조민수(M.S.Cho),노정용(J.Y.Moh),Li Jianwen(Li Jianwen),정영식(Y.S.Jeong) 한국자동차공학회 1997 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1997 No.11_1

In this study. a new diffusing-oriented spray combustion system for direct injection gasoline engines is refered. New instrument with multi-impingement walls, called the reflector. which is helpful to make air-fuel mixture, is used in this system as a strategy of forming air-fuel mixture. This is attached in front of fuel spray as the scheme of disintegrating fuel droplet injected from multi-hole injector and guiding impinged fuel spray to prefered direction. The impingement wall toward spark plug of reflector is specially designed. So the fuel spray toward spark plug is formed comparatively rich air-fuel mixture and the other fuel spray is formed stratification charge inside combustion chamber. <br/> The combustion system is investigated on a single cylinder four-stroke DI gasoline engine which is modified from a conventional DI diesel engine. The effects of the shape of reflector and injection timing are investigated without throttling on sfc, COV_imp, and HC emissions.<br/>

성층급기 직접분사식 가솔린기관 개발에 관한 연구 (Ⅱ) -연료분사압력 및 부하변동에 따른 연소특성 해석-

채재우(J.O.Chae),이상만(S.M.Lee),조민수(M.S.Cho),노정용(J.Y.Noh),Li Jianwen(Li Jianwen),정영식(Y.S.Jeong) 한국자동차공학회 1997 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1997 No.6_1

In general, the stratified charge for direct injection gasoline engine should be introduced to achieve ultra-lean combustion. In order to apply for the concept of stratified charge into direct injection gasoline engine, a reflector was adapted on cylinder head. An installation of the reflector in front of injector nozzle leads the mixture to be rich near spark plug. Therefore, the mixture near the spark plug is locally rich to ignite a fuel while the lean mixture is wholly introduced into the combustion chamber. In this paper, the characteristics of combus­tion is analyzed with the variations of injection pressure and load in a stratified-charge direct injection single cylinder gasoline engine. The results are summarized as follows ;<br/> 1. The MBT spark timing is influenced by engine rpm and load, but has a little relation with fuel injection pressure.<br/> 2. The MBT spark timing is approached to TDC with the increase of load on account of the increase of evaporation energy. Therefore, the peak pressure of cylinder is also delayed with the increase of load.<br/> 3. The stratification effect is cleared with the increase of injection. It is considered by the development of secondary diffusive combustion and the increase of heat release of same region, but proceed rapidly than diesel engine. Specially, in the case of high pressure inj-ection(170bar) and high load(3.0kgf ? m ), the diffusive combustion part is developed ex­cessively and results to the increase of peak pressure than middle load.<br/> 4. The index of engine stability, COV_inwp value, is generally decreased with the increase of load. Finally, the 150bar injection pressure is appeared as optimum in this research.<br/>

직접분사식 가솔린기관 개발에 관한 연구(Ⅰ)

채재우(J. O. Chae),이상만(S. M. Lee),차민혁(M. H. Cha),조민수(M. S. Cho),노정용(J. Y. Noh),Li Jianwen(Li Jianwen),정영식(Y. S. Jeong) 한국자동차공학회 1996 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1996 No.11_2

In general, the stratified charge for direct injection gasoline engine should be introduced to achieve ultra-lean combustion. In order to apply for the concept of stratified charge into direct injection gasoline engine, we adopted a reflector inside the cylinder head. An installation of the reflector in front of injector nozzle leads the mixture to be rich near spark plug. As mentioned above, the mixture near the spark plug is locally rich to ignite a fuel while the lean mixture is wholly introduced into the combustion chamber. As a result of the test, ultra-lean comustion could be achieved up to 57 : 1 of AIF ratio without swirl control unit at idle condition. In the case of installation of swirl control unit, considerably extended AIF ratio is expected. So far, the optimizations including analysis of exhaust gases, compromise for conflicting conditions, and fuel injection system have been studied.

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Li Weijian,Chen Gaohuang,Feng Zhenyu,Zhu Baoyi,Zhou Lilin,Zhang Yuying,Mai Junyan,Jiang Chonghe,Zeng Jianwen 한국유전학회 2021 Genes & Genomics Vol.43 No.12

Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Diferentially expressed mRNAs were identifed by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verifed that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

단기통 디젤기관에서 흡기과급이 연소와 배기가스특성에 미치는 영향에 관한 연구

채재우,Li Jianwen,이상만,정영식,박재근,최영준 대한환경공학회 1996 대한환경공학회 학술발표논문집 Vol.1996 No.12