Studies on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+ , P and ALP Activity in Osteoporosis-Induced Rats

Sang-keun Kim,Myung-hun Lee,Man-hee Rhee 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

on the Effects of Biomedicinal Agents on Serum Concentration of Ca2+, P andALP Activity in Osteoporosis-Induced RatsSang-keun Kim, Myung-hun Lee1 and Man-hee Rhee2College of Veterinary Medicine, Chungnam National University, 220 Gungdong, Yusong-Gu, Daejeon 305-764, Korea1National Veterinary Research and Quarantine Service, Anyang 430-824, Korea2College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, KoreaReceived April 3, 2003 / Accepted July 9, 2003J. Vet. Sci. (2003), 4(2), 151-154JOURNAL OFVeterinaryScience*Corresponding author: Sang-keun Kim Dept. of Vet. Med., Chungnam National University, Daejeon 305764, Korea Tel: +82-42-821-6754, Fax: +82-42-821-6754 E.-mail: kskkim@cnu.ac.kr

Streptomyces 속 KM-48 균주가 생성하는 Bacillus amyloliquefaciens 에 대한 항생물질

Lee, Sang-Man,Song, Bang-Ho 한국미생물 · 생명공학회 1983 한국미생물·생명공학회지 Vol.11 No.1

세포외 효소를 분필하는 Bacillus amyloliquefaciens의 생육을 선택적으로 저해하는 항생물질을 분리하고저 대구 근교의 토양시료 100여점을 채취하여 Streptomyces sp. KM-48주를 최종 선별하였다. 이 균주의 Nutrient Broth 배지에서의 배양00으로부터 활성성분을 acetone 유출, $Al_2$O$_3$, chromatography, avical TLC를 하여 UV 조사하에서 청록색 형광을 나타내는 단일물질로 정제하였다. 이 물질은 주로 그람양성균과 균모, 곰팡이 등에 강한 항균효과를 나타내었으며 Bacillus amyloliquefaciens에 대한 50% 생육저해농도가 18$\mu\textrm{g}$/$m\ell$이었다. 이 물질은 열에 비교적 약하였으며 산에는 강하게 그 활성이 유지되었다. 균주의 형태 및 배양성에서 Stretommces tsusimaensis의 유이균으로 동정되었다. One of Streptomyces sp. was screened as an antibiotic producing strain against Gram positive bacteria specifically from about one hundred Actinomycetes collections. The isolated KM-48 strain, indexed in our collection, was secreted an anbitiotic in nutrient broth culture. The active principle was extracted with acetone from the lyophilized solid of cultured filtrate. The nonadsorbed elute in aluminium oxide was isolated as a purified homogeneity which revealed a sing1e spot under ultra-violet beam on avicel thin layer chromatography. The isolated antibiotic was sensitive specifically against the Gram positive bacteria and fungi. About fifty percent inhibition of the control growth on Bacillus amyloliquefaciens was 18$\mu\textrm{g}$/$m\ell$. The antibiotic was lavile against thermotreatment and stabled in acidic condition remarkably. The KM-48 was identified as a similar strain to Streptomyces tsusimaensis on its morphological and cultural characteristics.

12주간의 유산소운동과 통근수단 전환이 비만 중년 남성의 신체구성과 혈중 지질에 미치는 영향

이소라(Lee, So-Ra),김예영(Kim, Yae-Yeong),정원상(Jung, Won-Sang),이만균(Lee, Man-Gyoon) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.2

The study was designed to investigate the effects of 12 weeks of aerobic exercise and change of commuting mode to public transportation on body composition and blood lipid profiles in middle-aged obese men. Forty subjects, aged 30-50 yrs, whose BMI was higher than 25 kg · m-2, were randomly assigned to one of three groups, i.e., aerobic exercise training group(AT; n=13), change of commuting mode group(CCM; n=14), and control group(CON; n=13). Subjects in the AT participated in aerobic exercise program, peformed at 40-75% of HRR, 15-35 min/session, 3 sessions/wk for 12 weeks, and subjects in the CCM changed the commuting mode from their own vehicle to public transportation 5 day/wk, 10 times/wk for 12 weeks. Subject in the CON were asked to maintain their normal life patterns during the same intervention period. Variables regarding body composition and blood lipid profiles were measured in pre-test and post-test, and the results were compared among three groups and between two tests. Main results of the present study were as follows: l) Walking amount increased significantly in only CCM as treated. 2) Body weight, waist circumference, waist-hip ratio, body mass index, body fat, and fat mass decrease significantly in the AT, while body weight, body mass index, body fat, and fat mass decreased significantly in the CCM (P〈.05), 3) LDL-C decreased significantly in the AT(P〈.01) and the CCM(P〈.05). It was concluded that 12 weeks of change of commuting mode to public transportation as well as aerobic exercise would be beneficial for improving variables regarding body composition and blood lipid profiles. Increment of physical activity by changing to public transportation from their own vehicle would be helpful to prevent obesity and dyslipidemia.

풍선 확장술로 치료한 담관결석과 동반된 총담관 막양구조

백성민 ( Sung Min Baek ),김동욱 ( Dong Uk Kim ),장선미 ( Sun Mi Jang ),송병구 ( Byeong Gu Song ),박종만 ( Jong Man Park ),이대성 ( Dae Sung Lee ),김중근 ( Joong Keun Kim ),황경림 ( Kyung Lim Hwang ) 대한췌장담도학회 2014 대한췌담도학회지 Vol.19 No.3

Bile duct web is very rare disease and it`s etiology is controversial. Some webs are occurred in the presence of chronic inflammation, frequently associated with bile duct stone, but others are thought to be congenital. Many patients with bile duct web are asymptomatic, but they sometimes present symptom of biliary obstruction and cholangitis. It can be diagnosed by endoscopic retrograde cholangiopancreatogram, typically appearing as thin and shelf like radiolucent ring. We report a case of the common bile duct web with bile duct stones diagnosed by Endoscopic retrograde cholangiopancreatography (ERCP) in a 65-year-old man. The patient was treated by balloon dilatation successfully.

1년간의 자발적인 운동참여가 중년 남성 제2형 당뇨병 환자의 혈중 지질 성분에 미치는 영향

허만동(Man Dong Huh),이진훈(Jin Hoon Lee),류승필(Sung Pil Ryu) 한국발육발달학회 2006 한국발육발달학회지 Vol.14 No.3

The purpose of this study is to investigate the effects of voluntary exercise participation on blood lipids in type II diabetes mellitus middle-aged men. Eighteen male volunteers were recruited and exercised, jogging, cycle ergometer, swimming, in the sports center for 12 months without any supervision. They had educated heart rate calculation method to keep their target heart rate during exercise. Before and after the training, their body composition, maximal oxygen uptake, and blood lipids profiles were analyzed. Body weight was not changed during experimental periods; however body fat ratio was statistically decreased at the same periods. Blood glucose, TC, LDL-C, and TG were decreased significantly and HDL-C was significantly increased (p<.05). RQ was significantly lower compared to the before exercise and their maximal oxygen consumption and exercise time to fatigue were increased significantly (p<.05). These results suggested that voluntary exercise with simple exercise prescription was also effectual to enhance blood lipids in type II diabetes mellitus middle-aged men.

Distribution and Potential Toxicological Effects of 2,2',4,4'-tetrabromodiphenyl Ether (BDE-47) as a Endocrine Disrupting Chemical in Human and Animals

Jung, Eui-Man,Yang, Hyun,An, Beum-Soo,Lee, Geun-Shik,Hyun, Sang-Hwan,Choi, Kyung-Chul,Jeung, Eui-Bae 韓國受精卵移植學會 2011 한국동물생명공학회지 Vol.26 No.4

Polybrominated diphenyl ethers (PBDEs) are a class of "brominated" (bromine containing) man-made chemicals used as flame retardant additives in plastics, foams, and textiles. PBDEs are found in various environmental contaminants in air, soil, sediment, and water, and 209 individual forms (congeners) of PBDE exist. Among these, 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) is the dominant congener found in the environment. Exposure to BDE-47 is now worldwide, and levels of BDE-47 have been detected in the blood of animals, including humans. BDE-47 can adversely affect the developmental system in both humans and animals. BDEs have structural similarities to polychlorinated biphenyls and thyroid hormones. However, recent studies have shown that BDEs may act as hormonal disrupting chemicals with detrimental effects. Therefore, a reliable assessment of BDE-47 toxicological action is required to understand the detrimental impacts of BDE-47 on human health. In this review, we overview recent studies on the distribution and potential toxicological effects of BDE-47 in humans and animals.

2011 대구세계육상선수권대회 남자 높이뛰기 메달리스트들의 바이오메카닉스적 특성 분석

김의환 ( Eui Hwan Kim ),배영상 ( Young Sang Bae ),김성섭 ( Sung Sup Kim ),권문석 ( Moon Seok Kwon ),위웅량 ( Ung Ryang Wo ),김기만 ( Ki Man Kim ),이정민 ( Jeong Min Lee ) 한국운동역학회 2011 한국운동역학회지 Vol.21 No.5

The purpose of this study was to perform a kinematic analysis of the high jump techniques of the three men`s medalists at the 2011 IAAF Championships in Daegu (August 27-September 4, 2011), In particular, a three-dimensional coordinates method was used to analyze the last three strides before touchdown, the touchdown techniques, and the movements after takeoff toward the bar. An analysis of the, data for the biomechanical characteristics of the world`s best high jumpers could contribute to an improvement in the performance of a national high jumper. The first conclusion of the data analysis was that the arm movements of the gold medalist, J. Williams, had a single arm form, whereas the arm movements of the other medalists were a double arm form. Second, the difference in the knee joint angles upon touchdown and toe-off was 10˚. Third, J. Williams achieved his maximum CM height after takeoff (1.26m) using the maximum flexion of his knee joint. Fourth, the foot contact duration of A. Dmitrik (0.11 s) was the shortest among the medalists, and the ratio for his transformation of horizontal velocity to vertical velocity was the greatest (75.25%) among the three. Last, the maximum CM height of T. Barry was the greatest, and his foot contact duration was the longest.

논문 : 댐붕괴 모형과 FLO-2D를 연동한 산대저수지 붕괴 침수 모의

고대홍 ( Dae Hong Go ),이길하 ( Khil Ha Lee ),김진만 ( Jin Man Kim ),김성욱 ( Sung Wook Kim ) 대한지질공학회 2015 지질공학 Vol.25 No.4

갑작스런 저수지 붕괴로 인하여 발생하는 자연재해를 관리하는 효율적인 방법은 홍수위험지도를 작성하는 것이다. 댐붕괴로 인한 홍수위험지도 작성에서 물리적인 현상을 재현해내기 위해서는 유출모형이 사용되는 것이 일반적이며, 모형을 이용하여 잠재적 피해대상지역을 사전에 파악하는데 모형의 정확도가 중요하다. 외국에서 만들어진 기존 상용모형을 검증을 거치지 않고 현장에 적용하는 것은 신뢰성에 문제가 있다. 따라서 모형 예측과 실측의 차이를 비교하여 모형의 정확도를 확인할 필요가 있다. 이 연구에서는 댐붕괴 모형을 하도추적모형인 FLO-2D모형에 연동하여 침수지역을 파악하였다. 모형의 매개변수는 모형의 결과에 중대한 영향을 미치므로 먼저 DEM을 구축한 후 토지피복도로 Manning계수를 산정하고 동시에 토양도를 사용하여 침투과정의 매개변수를 산정하였다. 모의 결과는 침수현장에서 설문조사를 통하여 제작한 현장침수지도와 상대 비교하였다. 침수지역의 수위와 범위 등을 비교한 결과 연구에 사용한 붕괴모형이 침수지역을 적절하게 재현해 내는 것으로 나타났다. The compilation of a flood hazard map is an efficient technique in managing areas at risk of flooding in the case of a dam-break. A scenario-based numerical modeling approach is commonly used to compile a flood hazard map related to dam-break and to determine the model parameters that capture peak discharge, including breach formation and progress, which are important in the modeling method. This approach might be considered less reliable if an existing model is used without local validation. In this study, a dam-break model is linked to a routing model to identify flood-risk areas in the case of failure of the Sandae Reservoir Gyeongju, Gyeongbuk. Model parameters are extracted from a DEM, and maps of land use and soil texture. The simulation results are compared with on-site investigations in terms of inundation and depth. The model reproduces the inundation zone with reasonable accu-racy.

폐기물 매립지반에 대한 동다짐공법 적용평가

김영묵,이상용,김만구,신승철,Kim, Young Muk,Lee, Sang Yong,Kim, Man Goo,Shin, Seung Cheol 대한토목학회 1993 대한토목학회논문집 Vol.13 No.5

본 고는 폐기물 매립지반의 지반개량을 위하여 1992년 3월 16일부터 1992년 10월 25일까지 수행된 동다짐공사 결과에 대한 연구사례이며, 개량대상지역은 대전시 갑천변에 위치하는 폐기물 매립부지로 1983년부터 1989년까지 주로 도시생활 쓰레기, 연탄재, 구조물 해체 잔해물 및 일부 산업폐기물로 매립되어 형성된 지역이다. 동다짐 공사는 2개 지점에서 시험동다짐을 시행하여 그 결과를 기준으로 수행되었으며, 개량효과 분석 및 품질관리를 위하여 각종 현장확인 시험 및 지반거동 계측을 수행하였다. 또한 동다짐시 인접된 구조물에 미치는 영향을 분석하기 위하여 진동측정을 시행하였다. 제반 현장시험 및 계측자료 분석결과 전체적으로 지반의 강도증가 및 압축효과가 양호한 것으로 확인되어 본 지역의 폐기물 매립지반에 대한 동다짐공법 적용은 효과적인 것으로 나타났으며, 향후 본 지역과 유사한 폐기물 매립지반에 대한 동다짐공법 적용시 참고가 될 수 있을 것으로 기대된다. This article is a case study of the ground improvement project which was carried out for manmade landfill. The project area is located near to Kapchun, Teajon and composed of the municipal wastes dumped, demolished building debris, coal ash and industrial waste made between 1983 and 1989. The DDC(dynamic deep compaction) based on the results of the test compaction at two representative locations was carried out from March 16, 1992 to Oct. 25, 1992. Field measurements and laboratory tests were carried out for ground improvement assessment and quality control for the DDC(dynamic deep compaction) work. From the results of field measurements and laboratory tests, it was found that the DDC work was successful: waste landfill was compressed considerably (${\fallingdotseq}$ 15% of full depth); and the strength was increased satisfactorily (${\fallingdotseq}$ 100% of original penetration resistance), Also, it is expected that the results of this work could be a guide to the future DDC work with the similar ground conditions, i.e. man-made landfills.

12주간의 유산소운동과 통근수단 전환이 중년 남성의 체력, 인슐린 저항성, 염증지표 및 간기능에 미치는 영향

허다솜 ( Da Som Heo ),김예영 ( Yae Young Kim ),정원상 ( Won Sang Jung ),이만균 ( Man Gyoon Lee ) 한국스포츠정책과학원(구 한국스포츠개발원) 2015 체육과학연구 Vol.26 No.1

선진 외국에서는 통근수단으로서 자가용 대신 대중교통을 이용함으로써 부가적으로 발생하는 신체활동이 건강수준에 미치는 효과를 밝히는 연구가 활발하게 이루어졌으나 국내의 관련 연구는 미흡한 실정이다. 이 연구의 목적은 12주간의 유산소운동과 대중교통으로의 통근수단 전환이 중년 남성의 체력, 인슐린 저항성, 염증지표 및 간기능에 미치는 영향을 규명하는 것이었다. 이 연구의 대상자는 최근 5년 이상 지속적으로 자가용을 이용해 온 체질량지수 25kg·m-2 이상의 30~50대의 사무직 중년 남성 44명이었다. 이들을 자가용을 계속 이용하면서 유산소운동을 실시한 운동집단(n=14), 자가용 이용을 중단하고 대중교통으로 전환한 전환집단(n=15), 그리고 자가용을 계속 이용하면서 특별한 처치 없이 기존의 생활 패턴을 유지한 통제집단(n=15)에 무선 할당하였다. 운동집단은 주 3회, 일일 30분의 유산소운동을 실시하였고, 전환집단은 통근수단을 대중교통으로 전환하여 주 5회, 일일 최소 20분의 보행을 유도하였다. 처치 전ㆍ후에 체력, 인슐린 저항성, 염증지표, 그리고 간기능과 관련된 변인을 측정한 후 반복 이원변량분석을 이용하여 집단 간, 그리고 검사 간에 비교하였다. 이 연구의 주요 결과는 다음과 같다. 1) 체력과 관련된 모든 변인이 운동집단에서 유의하게 향상되었고, 전환집단의 경우 오른손 악력, 제자리멀리뛰기, 사이드스텝, 그리고 앉아윗몸앞으로굽히기가 유의하게 향상되었다. 2) 인슐린 저항성과 관련된 모든 변인에서 통계적으로 유의한 변화가 나타나지 않았으나, 운동집단과 전환집단에서 개선되는 경향이 나타났다. 3) 염증지표와 관련하여 운동집단과 전환집단에서 TNF-α가 유의하게 감소되었으며, IL-6와 CRP가 개선되는 경향이 나타났다. 4) 간기능과 관련하여 전환집단에서 ALT가 유의하게 감소되었다. 이상의 결과를 종합해 보면, 12주간의 유산소운동이 체력과 염증지표 및 인슐린 저항성의 개선에 효과적인 것으로 나타났다. 아울러 통근수단을 자가용에서 대중교통으로 전환한 것만으로도 유산소운동 못지 않게 체력, 염증지표, 인슐린 저항성, 그리고 간기능에 긍정적인 영향을 미친 것을 알 수 있다. 시간이 부족하여 운동 참여가 줄어드는 현대인에게 있어서 대중교통으로 통근수단을 전환하는 시도는 일상생활에서 자연스럽게 신체활동량을 증가시키고 건강수준을 개선하는 대안이 될 수 있을 것으로 판단된다. This study was designed to investigate the effects of increment of physical activity for 12 weeks through aerobic exercise training or change from own vehicle to public transportation for commuting on physical fitness, insulin resistance, inflammatory markers, and liver function in middle-aged men. Forty-four subjects, aged 30-50 yrs, were randomly assigned to either one of three groups, i.e., aerobic exercise training group (TR: n=14), change to public transportation group (PT: n=15), or control group (CON: n=15). Subjects in TR performed aerobic exercise for 30 min per sessions, three sessions per week, subjects in PT changed from their own vehicle to public transportation for commuting, and subjects in CON maintained their life patterns during the same intervention period. Physical fitness, insulin resistance, inflammatory markers, and liver function were measured at pre- and post-test, and the data were analyzed by repeated two-way ANOVA. Main results of the present study were as follows: 1) All variables related to physical fitness improved significantly in TR. Right grip strength, standing long jump, side step test, and sit-and-reach improved significantly in PT. 2) Although there were no significant changes in all variables related to insulin resistance, the variables tended to be improved in TR and PT. 3) TNF-α decreased significantly in TR and PT. IL-6 and CRP tended to be improved in TR and PT; however, the changes did not reach statistical significant level. 4) ALT decreased significantly in PT. AST and γ-GT tended to be improved in TR and PT; however, the changes did not reach statistical significant level. It was concluded that the 12 weeks of change to public transportation as well as aerobic exercise training would be beneficial for physical fitness and inflammatory markers. These interventions also would be possible to improve insulin resistance and liver function. The increment of physical activity through change from own vehicle to public transportation was found to be equally beneficial for health promotion compared to aerobic exercise.