Nutlin-3 induces HO-1 expression by activating JNK in a transcription-independent manner of p53

CHOE, YUN-JEONG,LEE, SUN-YOUNG,KO, KYUNG WON,SHIN, SEOK JOON,KIM, HO-SHIK Spandidos Publications 2014 International journal of oncology Vol.44 No.3

A recent study reported that p53 can induce HO-1 by directly binding to the putative p53 responsive element in the HO-1 promoter. In this study, we report that nutlin-3, a small molecule antagonist of HDM2, induces the transcription of HO-1 in a transcription-independent manner of p53. Nutlin-3 induced HO-1 expression at the level of transcription in human cancer cells such as U2OS and RKO cells. This induction of HO-1 did not occur in SAOS cells in which p53 was mutated and was prevented by knocking down the p53 protein using p53 siRNA transfection, but not by PFT-alpha, an inhibitor of the transcriptional activity of p53. Accompanying HO-1 expression, nutlin-3 stimulated the accumulation of ROS and the phosphorylation of MAPKs such as JNK, p38 MAPK and ERK1/2. Nutlin-3-induced HO-1 expression was suppressed by TEMPO, a ROS scavenger, and chemical inhibitors of JNK and p38 MAPK but not ERK1/2. In addition, nutlin-3-induced phosphorylation of JNK but not p38 MAPK was inhibited by TEMPO. Notably, the levels of nutlin-3-induced ROS were correlated with the mitochondrial translocation of p53 and this induction was prevented by PFT-beta, an inhibitor of the mitochondrial translocation of p53. Consistent with the effect of the ROS scavenger and MAPK inhibitors, PFT-beta reduced HO-1 expression and the phosphorylation of JNK induced by nutlin-3. In the experiments of analyzing cell death, the knockdown of HO-1 augmented nutlin-3-induced apoptosis. Collectively, these results suggest that nutlin-3 induces HO-1 expression via the activation of both JNK which is dependent on ROS generated by p53 translocated to the mitochondria and p38 MAPK which appears to be stimulated by a ROS-independent mechanism, and this HO-1 induction may inhibit nutlin-3-induced apoptosis, constituting a negative feedback loop of p53-induced apoptosis.

CMOS 연산 증폭기 및 비교기 회로의 자동 설계

신현우,김천호,김득경,신창호,신경욱 國立金烏工科大學校附設生産技術硏究所 1994 産業技術開發硏究 Vol.10 No.-

The paper describes a CMOS analog circuit design tool, K_ACD(Kumoh_Analog Circuits Designer), and design results. The K_ACD was developed for automatic design of fundamental analog circuit blocks such as comparator, unbuffered OP AMP, and buffered OP AMP. For given specifications and process parameters, it computes bias currents, transistor sizes, voltage gain, and power dissipation, then SPICE simulations are automatically carried out. Some design examples show usefulness of the K_ACD in analog circuit designs.

한국형 고속전철 집전 시스템의 동적설계에 관한 연구

허신(Hur Shin),경진호(Kyung Jin-Ho),송달호(Song Dahl-Ho) 한국철도학회 1998 한국철도학회 학술발표대회논문집 Vol.- No.-

The dynamic performance design of catenary-pantograph system which collects current for the next generation Korean high speed train(KHST) was considered. Used was the same dynamic model of the catenary-pantograph system as that of TGV-K which will be introduced for Kyung-bu corridor. Using the model, sensitivity analysis for design variables were made to improve dynamic performance of KHST system. The results of sensitivity analysis and performance improvement are as follows: (1) It was found that aerodynamic force, tension of contact wire, mass of contact strip, mass of supporting contact strip, mass of clamp, mass of steady arm, and stiffness of plunger were the design variables most influencing the dynamic performance of the system. (2) Pantograph with reductions of 20% aerodynamic force, 34% weight of supporting contact strip, 20% spring constant of plunger, and 34% equivalent mass of steady arm was very possible system for the KHST which will be running at maximum operating speed 350 ㎞/h.

인쇄매체를 이용한 보건교육자료의 가시성에 영향을 주는 요인에 관한 연구

신준호,정은경,이정애,오승진,김신월 全南大醫大 2001 전남의대학술지 Vol.37 No.2

디지털 무선 모뎀용 적응 결정귀환 등화기 설계

신경욱,안병규,김호하 金烏工科大學校 1998 論文集 Vol.19 No.-

This work describes an ASIC implementation of adaptive decision-feedback equalizer (ADFE) which is applicable to wide band digital wireless modems. A new approach based on redundant binary (RB) arithmetic is devised for an efficient implementation of complex-number filter which is a key functional block of ADFE. The proposed RB complex-number filter structure reduces the critical path delay of ADFE. as well as leads to a more compact realization than conventional methods. Also, the carry-propagation free (CPF) operations of the RB arithmetic enhance its speed. To demonstrate the proposed approach, a prototype chip set which is composed of feed-forward equalizer module (FFEM) and decision-feedback equalizer module (DFEM) has been designed. Each module contains two complex-number filter taps along with their coefficient update circuits, and can be cascaded to implement longer filter taps for high bit-rate applications. The chip set was modeled and verified using VHDL. and synthesized using 0.8-㎛ sea-of-gate (SOG) array library.

鷄龍山 森林土壤의 特性에 關하여

辛昌男,宋鎬京,朴鍾聲 忠南大學校 環境問題硏究所 1983 環境硏究 Vol.1 No.2

In order to determine the characteristics of soil in Mt. Gyeryong, the content of primary minerals and chemical and physical properties of forest soil were analyzed. The parent rock of forest soil were consisted of pink feldspar granite and gneissose granite. The weathered parent rock contained about 64% of plagioclase and quartz. The content of available phosphorus of soil resulted from weathering process of pink feldspar granite was higher than that of the gneissose granite soil. But the contents of K and of the latter were higher than those of the former. Both types of soil texture were mainly loam and clay loam The differences of chemical soil properties between Pinetum consociation and Quercetum consociation were as follows:the contents of the organic matter, total exchangeable bases, NO^-_3-N and NH^+_4-N of Quercetum consociation were higher than as compared to those Pinetum consociation in the pink feldspar granite region, the contents of total nitrogen, available phosphorus, total exchangeable bases, Mg^++ and NO^-_3-N of Quercetum consociation were higher than as compared to those of Pinetum consociation in the gneissose granite region.

대요근 근구 차단술의 해부학적 고찰 및 임상 효과

신경호,김헌,최락민,김희상,안경회 大韓再活醫學會 2000 Annals of Rehabilitation Medicine Vol.24 No.2

우수방사성의약품 생산시설 개발

신병철,정원명,박상현,이규일,박경배,박진호 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

Manufacturing facilities of the pharmaceuticals must meet certain level of the cleanness required so that foreign substances such as dust, moisture, heat, microorganism, or virus do not contaminate the product. In case of radiopharmaceuticals for medical treatment and diagnosis, not only should the operators and environment be protected from radiation but also need to be isolated from the foreign contaminant. Therefore, manufacturing facilities for radiopharmaceuticals must satisfy the design standards of both hot cell and clean room which are specified by GNP. However, standards of maintaining negative pressure for preventing spread of radioactive contaminant in isolated facilities conflict with the standards of maintaining positive pressure for keeping cleanness. To solve this problem, are pressure of hot cell was designed lower than in the adjacent area to meet standards of the radiation safety. To keep higher cleanness in certain part of the hot cell for filling, minimal relative positive pressure allows. In order to effectively maintain the cleanness that is required for production of Tc-99m generator, which takes 70% of whole demand of radiopharmaceuticals, the rooms placed in each side of production room are used as a buffer area and three lead hot cells are installed in production room. In this research, we established the appropriate engineered design concept for Tc-99m generator manufacturing facility, which satisfies both GMP cleanness standard for preventing particles, bacteria, other contaminants and the regulations of radiation safety for supervising and controlling the amount of radiation exposure and exhausted radioactivity. And the concept of multi-buffer zones is introduced to apply negative air pressure for hot cell with first priority and to continue relative positive air pressure for clean room.

교육투자가 임금에 미치는 효과에 관한 실증분석

신경수,송일호 東國大學校 社會科學硏究院 2005 사회과학연구 Vol.12 No.1

The purpose of this study is to estimate the effect of educational investment on wage. According to the empirical results, the wage gaps for each education level are observed significantly, because the higher education level means to get more earnings. Moreover, the wage premium of middle school is almost vanished. This study also explores the marginal rate of returns of educational investment. First of all, the marginal rates of returns in high school and university have drastically declined since 2000. The marginal rates of returns in college graduates has drastically increased since 2000. Secondly, the marginal rate of returns of female is superior to that of male. Third, as the marginal rate of returns to education in middle school is decreased rapidly, that is almost vanished at present. In addition, this study estimates the marginal rate of returns to education by education level. The marginal rate of returns of university graduates is the highest, and that of high school graduates is better than that of college graduates. It also shows that the marginal rates of returns of college graduates exceeds that of university graduates. Finally, this study shows that the academic wage gap, that was declining after 1980's, has been increased since mid of 1990's. Second, the academic wage gap of female is higher than that of male.

三天地의 環境과 生物多樣性 : I. Enviornmental Factors and Flora I. 環境要因과 植物相에 關하여

신상천,이종운,배규호,전재인,강경화 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-

Environmental factors and phytological aspects of Samchunimpoundment in Kyongsan, Kyongpook were studied. 40 quadrats in 4 sites had 94% herbaceous cover, 31% shrub cover and 18% tree cover. Ambrosia artemisiifolia var. elatior, Metaplexis japonica, Erigeron annuus, Humulus Japonicus, Eragrostis ferruginea, Artemisia princeps var. orientalis were dominant herbaceous species and found cultivated plants of Hordeumn vulgare var. hexastichon, Prunus persica, Morus alba and neophytes of Ambrosia artemisiifolia var. elatior, Erigeron canadensis, Erigeron annuus, Ailanthus altissima, Bidens bipinnata, Lactuca indica var. laciniata, Robinia pseudo-acacia, oenothera odorata, Euphobia maculata. The investigated sites were divided into 4 patterns based on the vegetation with high difference level and Ambrosia artemisiifolia var. elatior, Medicago sativa, Setaria viridis, Metaplexis japonica, Humulus japonicus, Calystegia japonica, Forsythia koreana, Lsctuca indica var. laciniata were different species group. More than 90% on the surface area of water mass covered with Nelumbo nucifera, Marsilea quadrifolia and Typha angustata and distributed Hydrilla verticillata, Ceratophyllum demersum, Trypa pseudo-incisa, Myriophyllum spicatum under water. Average data of environmental factor analysis are as followings; air temperature 25.8℃, ph 6.77, total hardness 117.94㎎/ℓ, SS 4.5㎎/ℓ,Do 3.3㎎/ℓ,BOD 6.6㎎/ℓ,COD 7.2㎎/ℓ,cl- 17.56㎎/ℓ,?? 7.14㎎/ℓ,Mn 0.05㎎/ℓ, Zn 0.04㎎/ℓ. Total quality of impoundment water were heavily polluted and were quality class Ⅳ.