Real-time imaging of glioblastoma using bioluminescence in a U-87 MG xenograft model mouse

Kim, Woong,Kang, Bo Ram,Kim, Hye Yun,Cho, Soo Min,Lee, Yong-Deok,Kim, Sehoon,Kim, Jung Young,Kim, Dong Jin,Kim, YoungSoo The Korean Society for Applied Biological Chemistr 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.2

Glioblastoma multiforme (GBM), the most common malignant brain tumor, is characterized by aggressive proliferation and invasive potential. Xenograft animal models of GBM have critically contributed to evaluation of novel therapeutic agents, drug delivery system, and diagnostic tools. To mimic intrinsic behavior of GBM, orthotopic transplantation of cancer cells and continuous observation of cell growth should be conducted in animal study. Here, we generated xenograft model mouse of GBM in which U-87 MG human glioblastoma cells were intracranially implanted for live imaging. Introducing luciferase gene into U-87 MG cell line enabled real-time observation and quantification of tumor survival and propagation by detecting photon emission derived from luciferase. Our GBM model mouse has potentials to bring great advantages in pharmacological and mechanistic investigation on brain tumors.

Characteristics of kidney transplantation recipients over time in South Korea

Sehoon Park,김명석,Ji Eun Kim,Kwangsoo Kim,Minsu Park,Yong Chul Kim,Kwon Wook Joo,Yon Su Kim,Hajeong Lee 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.6

Background/Aims: Detailed nationwide information regarding the recent status and time trends of kidney transplantation (KT) in South Korea is limited. Methods: We performed a nationwide, population-based cohort study using the national claims database of Korea. We included KT recipients from 2008 to 2016, and their demographic and clinical characteristics were collected. The prognostic outcome was graft failure consisted of patient death and death-censored graft failure (DCGF). Results: We studied 14,601 KT recipients with median follow-up duration of 3.96 years. The median age at the time of transplantation consistently increased from the past, and proportion of underlying diabetes mellitus prominently increased, reaching 35.6% in 2016. The preemptive KT accounted for approximately 30% of the total transplantation cases. The recipients showed a 10-year cumulative graft survival rate of 71.8%, consisting of 10-year DCGF free survival of 77.6% and patient survival of 92.8%. Age ≥ 20 and < 30 years, age ≥ 70 years, underlying history of diabetes, non-preemptive transplantation, and poor compliance on tacrolimus and mycophenolic acid were the significant risk factors associated with worse DCGF outcome. The economic cost of KT showed prominently increasing trends, reaching a total insured fee of > 60,000,000$ in 2016. However, the expansion was mainly burdened by the national insurance service but not by the patients. Conclusions: In South Korea, the number of kidney transplantation in elderly or in patients with comorbidities has been increasing. Complex clinical factors were associated with medication compliance and patient prognosis.

Epidemiology of continuous renal replacement therapy in Korea: Results from the National Health Insurance Service claims database from 2005 to 2016

( Sehoon Park ),( Soojin Lee ),( Hyung Ah Jo ),( Kyungdo Han ),( Yaerim Kim ),( Jung Nam An ),( Kwon Wook Joo ),( Chun Soo Lim ),( Yon Su Kim ),( Hyeongsu Kim ),( Dong Ki Kim ) 대한신장학회 2018 Kidney Research and Clinical Practice Vol.37 No.2

Background: Continuous renal replacement therapy (CRRT) is an important treatment modality for severe acute kidney injury. As such, the epidemiology of CRRT in Korea needs further investigation. Methods: We conducted a nationwide, population-based study analyzing the claims data from National Health Insurance Service of Korea. All index intensive care unit admission cases of CRRT in government-designated tertiary referral hospitals in Korea from 2005 to 2016 were included. Patients with a history of renal replacement therapy or who were under 20 years old were not considered. In addition to baseline and treatment characteristics, patient outcomes, including all-cause mortality and renal survival rates, were investigated. We stratified the study patients according to 3-year time periods and major regions of the nation. Results: We included 37,337 patients who received CRRT in Korea. The overall use of CRRT increased over time, and more than 80% of cases of acute renal replacement therapy were CRRT after 2014. Seoul was the region in which the majority of CRRT (45.0%) was performed. The clinical characteristics of CRRT patients were significantly different among time-intervals and regions. Both all-cause mortality and renal survival rates after CRRT were prominently improved in the recent time periods (P < 0.001). Conclusion: CRRT is a widely used treatment strategy for severe acute kidney injury in Korea. The prognosis of CRRT patients has improved compared to the past. This epidemiological study of CRRT in Korea revealed notable trends with regard to time period and geographic region.

Genetic variations in HMGCR and PCSK9 and kidney function: a Mendelian randomization study

( Sehoon Park ),( Seong Geun Kim ),( Soojin Lee ),( Yaerim Kim ),( Semin Cho ),( Kwangsoo Kim ),( Yong Chul Kim ),( Seung Seok Han ),( Hajeong Lee ),( Jung Pyo Lee ),( Kwon Wook Joo ),( Chun Soo Lim ) 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.4

Background: The genetically predicted lipid-lowering effect of HMGCR or PCSK9 variant can be used to assess drug proxy effects on kidney function. Methods: Mendelian randomization (MR) analysis-identified HMGCR and PCSK9 genetic variants were used to predict the low-density lipoprotein (LDL) cholesterol-lowering effects of medications targeting related molecules. Primary summary-level outcome data for log-estimated glomerular filtration rate (eGFR; creatinine) were provided by the CKDGen Consortium (n = 1,004,040 European) from a meta-analysis of CKDGen and UK Biobank data. We also conducted a separate investigation of summary-level data from CKDGen (n = 567,460, log-eGFR [creatinine]) and UK Biobank (n = 436,581, log-eGFR [cystatin C]) samples. Summary-level MRs using an inverse variance weighted method and pleiotropy-robust methods were performed. Results: Summary-level MR analysis indicated that the LDL-lowering effect predicted genetically by HMGCR variants (50-mg/dL decrease) was significantly associated with a decrease in eGFR (-1.67%; 95% confidence interval [CI], -2.20% to - 1.13%). Similar significance was found in results from the pleiotropy-robust MR methods when the CKDGen and UK Biobank data were analyzed separately. However, the LDL-lowering effect predicted genetically by PCSK9 variants was significantly associated with an increase in eGFR (+1.17%; 95% CI, 0.10%-2.25%). The results were similarly supported by the weighted median method and in each CKDGen and UK Biobank dataset, but the significance obtained by MR-Egger regression was attenuated. Conclusion: Genetically predicted HMG-CoA reductase inhibition was associated with low eGFR, while genetically predicted PCSK9 inhibition was associated with high eGFR. Clinicians should consider that the direct effect of different types of lipid-lowering medication on kidney function can vary.

SWAT과 MODSIM-DSS를 이용한 금강유역의 미래농업용수 공급능력 평가

김세훈(Kim, Sehoon),정충길(Jung, Chunggil),이지완(Lee, Jiwan),김진욱(Kim, Jinuk),김성준(Kim, Seongjoon) 한국방재학회 2020 한국방재학회논문집 Vol.20 No.6

본 연구는 SWAT과 MODSIM-DSS를 이용하여 금강유역(9,865 km²)의 향후 농업용수 공급능력 평가를 수행하였다. 농업용수 공급능력평가를 위한 물수지 분석은 MODSIM-DSS를 이용하여, 농업용 수리시설의 용수공급과 및 유역의 수요현황을 고려하였다. 14개중권역으로 구분된 유역 및 농업용 저수지 유입량(공급량) 자료는 SWAT 모형의 소유역별 유출결과를 사용하였다. SWAT 모형의검·보정은 금강유역 내 위치한 다목적댐(용담, 대청)과 보(백제보, 세종보, 공주보) 지점에서 실시하였다. 그 결과 2개 댐의 유입량및 저수량과 3개의 보에서 Nash-Sutcliffe의 모형효율계수(NSE)은 각각 0.55 ~ 0.70, 0.57 ~ 0.77로 검·보정되었다. 이후 SWAT 모형의유출결과를 MODSIM-DSS에 적용하여 물수지 분석을 수행하였다. 그 결과 1982년, 1988년, 1994년, 2001년, 2015년 기간에 농업용수부족량이 크게 발생하는 것으로 나타났으며, 14개 소유역 중 3004(영동천) 및 3012(금강공주) 유역에 농업용수 부족량이 각각 25.1 × 10⁶ m³, 47.4 × 10⁶ m³로 크게 발생하였다. 최종적으로 RCP 8.5 INM-CM4 시나리오를 적용하여 미래의 농업용수 부족량을 분석한결과 3004 및 3012 유역은 2080s (2070-2099) 기간에 26.1 × 10⁶ m³, 50.9 × 10⁶ m³로 용수 부족량이 증가하는 것으로 분석되었다. This study is to evaluate future agricultural water supply capacity in Geum river basin (9,865 km²) using SWAT and MODSIM-DSS. The MODSIM-DSS was established by dividing the basin into 14 subbasins, and the irrigation facilities of agricultural reservoirs, pumping stations, diversions, culverts and groundwater wells were grouped within each subbasin, and networked between subbasins including municipal and industrial water supplies. The SWAT was calibrated and validated using 11 years (2005-2015) daily streamflow data of two dams (DCD and YDD) and 4 years (August 2012 to December 2015) data of three weirs (SJW, GJW, and BJW) considering water withdrawals and return flows from agricultural, municipal, and industrial water uses. The Nash−Sutcliffe efficiency (NSE) of two dam and three weirs inflows were 0.55∼0.70 and 0.57∼0.77 respectively. Through MODSIM-DSS run for 34 years from 1982 to 2015, the agricultural water shortage had occurred during the drought years of 1982, 1988, 1994, 2001 and 2015. The agricultural water shortage could be calculated as 197.8 × 10⁶ m³, 181.9 × 10⁶ m³, 211.5 × 10⁶ m³, 189.2 × 10⁶ m³ and 182.0 × 10⁶ m³ respectively. The big shortages of agricultural water were shown in water resources unit map number of 3004 (Yeongdongcheon) and 3012 (Geumgang Gongju) areas exceeding 25.1 × 10⁶ m³ and 47.4 × 10⁶ m³. From the estimation of future agricultural water requirement using RCP 8.5 INM-CM4 scenario, the 3004 and 3012 areas showed significant water shortages of 26.1 × 10⁶ m³ (104.1%) and 50.9 × 10⁶ m³ (107.4%) in 2080s (2070∼2099) compared to the present shortages. The water shortages decreased to 23.6 × 10⁶ m³ (94.0%) and 43.3 × 10⁶ m³ (91.4%) below of the present shortages by developing irrigation facilities.

Lung Transplantation in Six Patients with Idiopathic Pulmonary Artery Hypertension

( Hyeonhwa Kim ),( Dongkwan Kim ),( Sehoon Choi ),( Geundong Lee ),( Dongkyu Oh ),( Hocheol Kim ),( Jaeseung Lee ),( Sungho Jung ),( Pilje Kang ),( Won Kim ),( Seungil Park ),( Sangbum Hong ) 대한결핵 및 호흡기학회 2021 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.129 No.0

Idiopathic pulmonary artery hypertension (IPAH) is an incurable and invariably fatal disease. Lung transplantation is a useful therapeutic option in patients who are unresponsive to medical treatment; however, lung transplantation performed for pulmonary hypertension is associated with significantly high perioperative mortality rates. We report a case series of six patients who underwent lung transplantation for IPAH between October 2008 and June 2021. Patients’ median age was 28.5 years, and the study included 5 of 6 women (83%). Pre-transplantation hemodynamic parameters showed mean right atrial pressure of 12.0±7.1 mmHg and mean pulmonary artery pressure of 62.2±29.5 mmHg. Two of six patients received extracorporeal membrane oxygenation (ECMO) therapy as a bridge to transplantation over 14 and 17 days, and four patients underwent elective transplantation. Two patients required prolonged postoperative venoarterial (V-A) ECMO support. Grade 3 primary graft dysfunction occurred in one patient; however, the clinical course improved following prolonged V-A ECMO therapy. Five patients (83.3%) required intervention for postoperative bleeding control; one of these patients died of uncontrolled bleeding concomitant with disseminated intravascular coagulation, on the 14th postoperative day, and we observed no other perioperative deaths. One patient died of carbapenem-resistant Acinetobacter baumannii bacteremia, a year postoperatively. The 1-month, 6-month, and 1-year survival rates were 83.3%, 83.3%, and 66.7%, respectively. In view of the poor prognosis of IPAH, lung transplantation (1-year mortality rates <40%) merits consideration as a useful therapeutic option in this patient population. However, postoperative bleeding tends to occur in most patients; therefore, close monitoring is important during post-transplantation management.

SWAT과 MODSIM-DSS 모형을 이용한 미래 농업용 지하수 공급능력 평가-금강유역을 대상으로-

김세훈 ( Sehoon Kim ),이지완 ( Jiwan Lee ),김진욱 ( Jinuk Kim ),김성준 ( Seongjoon Kim ) 한국농공학회 2019 한국농공학회 학술대회초록집 Vol.2019 No.-

국가적 수자원 계획이 수립되기 위해서는 기본적으로 물공급 안전도 평가와 유출량 및 수요량을 대비하는 물수지 분석이 선행되어야 한다. 특히 농업가뭄에 대한 대책 마련을 위해서는 가뭄기간 동안의 농업용 수리시설의 용수공급 가능량 파악이 매우 중요하다. 본 연구는 지하수이용량 자료를 고려한 SWAT 및 MODSIM 모형을 이용하여 금강유역(9,865㎢)의 향후 농업용수 공급능력 평가를 수행하였다. 하천유역 네트워크 물수지 모형인 MODSIM 모형은 금강유역을 14개의 소유역으로 구분하고 다목적댐과 농업용 수리시설을 고려한 물수지 분석을 수행하였다. 모형의 유역별 유입량(공급량) 자료는 SWAT 모형의 소유역별 유출결과를 사용하였다. SWAT 모형의 검보정은 11년(2005-2015) 동안의 유역내 2개 다목적댐(대청, 용담)과 3년 5월(2012년 8월∼2015년 12월) 동안의 3개 보지점(세종보, 공주보, 백제보)의 일별 유입량 및 저수량 자료를 이용하였다. 2개 댐의 유입량 및 저수량 검보정 결과 Nash-Sutcliffe의 모형효율계수(NSE)와 결정계수(Coefficient of determination, R²)는 각각 0.55∼0.70, 0.67∼0.75이며, 3개의 보에서의 NES 및 R²는 0.57∼0.77, 0.62∼0.81로 검보정 되었다. 이후 MODSIM-DSS를 이용하여 35개년(1981∼2015) 동안의 물수지 분석을 수행한 결과 과거 실제로 가뭄이 발생했던 기간인 1982년, 1988년, 1994년, 2001년, 2008년, 2015년에 농업용수 부족량이 평년에 비해 많이 발생하는 것으로 나타났으며, 부족량은 각각 142 10⁶㎥, 117 10⁶㎥, 149 10⁶㎥, 129 10⁶㎥, 113 10⁶㎥, 114 10⁶㎥로 분석되었다. 향후 미래 농업용수 부족량 현상을 파악해 필요한 물 사용량을 충족하기 위한 농업용수 공급시설의 가능한 방법을 재현할 수 있을 것으로 예상된다.

금강 유역의 농업용수 건전성 및 취약성 평가

김세훈 ( Sehoon Kim ),이지완 ( Jiwan Lee ),김진욱 ( Jinuk Kim ),한대영 ( Daeyoung Han ),김성준 ( Seongjoon Kim ) 한국농공학회 2021 한국농공학회 학술대회초록집 Vol.2021 No.-

Simple Postoperative AKI Risk (SPARK) Classification before Noncardiac Surgery: A Prediction Index Development Study with External Validation

Park, Sehoon,Cho, Hyunjeong,Park, Seokwoo,Lee, Soojin,Kim, Kwangsoo,Yoon, Hyung Jin,Park, Jiwon,Choi, Yunhee,Lee, Suehyun,Kim, Ju Han,Kim, Sejoong,Chin, Ho Jun,Kim, Dong Ki,Joo, Kwon Wook,Kim, Yon Su American Society of Nephrology 2019 Journal of the American Society of Nephrology Vol.30 No.1

Reduced Tacrolimus Trough Level Is Reflected by Estimated Glomerular Filtration Rate (eGFR) Changes in Stable Renal Transplantation Recipients: Results of the OPTIMUM Phase 3 Randomized Controlled Study

Park, Sehoon,Kim, Yon Su,Lee, Jungpyo,Huh, Wooseong,Yang, Chul Woo,Kim, Young-Lim,Kim, Yeong Hoon,Kim, Joong Kyung,Oh, Chang-Kwon,Park, Su-Kil International Scientific Literature, Inc. 2018 Annals of transplantation Vol.23 No.-

<P><B>Background</B></P><P>Minimizing the tacrolimus dosage in patients with stable allograft function needs further investigation.</P><P><B>Material/Methods</B></P><P>We performed an open-label, randomized, controlled study from 2010 to 2016 in 7 tertiary teaching hospitals in Korea and enrolled 345 kidney transplant recipients with a stable graft status. The study group received reduced-dose tacrolimus, 1080–1440 mg/day of enteric-coated mycophenolate sodium (EC-MPS), and corticosteroids. The control group received the standard tacrolimus dosage and 540–720 mg/day of EC-MPS with steroids. The primary endpoint was the mean estimated glomerular filtration rate (eGFR) and change in the eGFR at 12 months after randomization.</P><P><B>Results</B></P><P>The mean tacrolimus trough level of the study group was 4.51±1.62 ng/mL, which was lower than that of the control group, at 6.75±2.82 ng/mL (P<0.001). The primary endpoint was better in the study group in terms of change in eGFR (P<0.001). The month 12 eGFRs were 73.6±28.4 and 68.3±18.1 mL/min/1.73 m<SUP>2</SUP> in the study and the control groups, respectively, but the difference did not reach statistical significance (P=0.07). The incidence of adverse events was similar between the study and the control groups.</P><P><B>Conclusions</B></P><P>Minimizing tacrolimus to a trough level below 5 ng/mL combined with conventional EC-MPS can be considered in patients with a steady follow-up, as it was associated with small benefits in the changes of the eGFR (Clinicaltrials.gov number: NCT01159080).</P>