Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

眞智王의 卽位에 대한 再檢討

金德原(Kim, Deok-Won) 백산학회 2002 白山學報 Vol.- No.63

There has been little substantial progress in the study of King Jin Ji (眞智王). This is due to a shortage of records regarding his reign that resulted from the political turmoil leading up to his dethronement four years after taking the throne. Negative impressions implanted in the process of evaluating and recording his administrative achievements in comparison to King Jin Pyeong(眞平王) are another reason for this dearth of records. The background information that made it possible for him to become King, however, highlights both his personal ability and internal and external circumstances unique to the era of his accession. In terms of ability, his age was considered an important fact qualifying him to be King, as was his renown as a wise statesman with a keen and accurate sense of judgment. Taking into account the internal and external circumstances both before and after he became king, one must consider the Shilla Kingdom (新羅) suffering frequent attacks mounted by the Paekje Kindgom(百濟) externally given the inroads that King Jin Heung(眞興王) made into the Hang River(漢江) basin. The ideology of Cakravartirajan(轉輪聖王) late emerged as an internal factor, in terms of how it was closely connected to the Buddhist politics of King Jin Heung(眞興王). It was Geo Chil-bu(居柒夫) who played a pivotal role in bringing Jin Ji to the throne. He had been characterized as a symbolic figure that made great contributions to the expansion of Shilla Kingdom(新羅) territory by becoming a member of inner Buddhist circles somewhat earlier than others. In this context he was able to assist Jin Ji's accession to the throne in the following ways: Safeguarding the Shilla Kingdom territory from external threats was always an imminent proposition, while the internal politics of the Kingdom involved a pursuit of inheriting the Buddhist policies of King Jin Heung(眞興王). In addition to the Geo Chil-bu's(居柒夫) loyalty played in the coronation of King Jin Ji(眞智王), the role Kim Mu-ryeok(金武力) played is also worthy of attention. It has been inferred from what few records are available, that the military stronghold Kim Mu-ryeok had in cooperation with Geo Chil-bu(居柒夫) was a major factor in Jin Ji's accession to the throne. The relationship between King Jin Ji(眞智王) and Kim Mu-ryeok(金武力) also played had a significant influence on their kinsfolk. It has, therefore, been suggested that as a result King Yong-chun(金龍春) was nudged toward ties with Kim Seo-hyeon(金舒玄), and Kim Chun-chu(金春秋) affiliated himself with Kim Yu-sin(金庾信). It was perhaps these Saryun(舍輪系) and Kaya(加耶系) clique relationships, upon which a new aristocracy that has come to be known as the spread of ‘the middle ages(中代)’ was based.

Charge Spreading Effect of Stored Charge on Retention Characteristics in SONOS NAND Flash Memory Devices

Kim, Seong-Hyeon,Yang, Seung-Dong,Kim, Jin-Seop,Jeong, Jun-Kyo,Lee, Hi-Deok,Lee, Ga-Won The Korean Institute of Electrical and Electronic 2015 Transactions on Electrical and Electronic Material Vol.16 No.4

This research investigates the impact of charge spreading on the data retention of three-dimensional (3D) silicon-oxide-nitride-oxide-silicon (SONOS) flash memory where the charge trapping layer is shared along the cell string. In order to do so, this study conducts an electrical analysis of the planar SONOS test pattern where the silicon nitride charge storage layer is not isolated but extends beyond the gate electrode. Experimental results from the test pattern show larger retention loss in the devices with extended storage layers compared to isolated devices. This retention degradation is thought to be the result of an additional charge spreading through the extended silicon nitride layer along the width of the memory cell, which should be improved for the successful 3-D application of SONOS flash devices.

Salvage living donor liver transplantation for post-resection recurrence of combined hepatocellular carcinoma-cholangiocarcinoma

Kim Minjae,Hwang Shin,Song Gi-Won,Ahn Chul-Soo,Moon Deok-Bog,Jung Dong-Hwan,Park Gil-Chun,Lee Sung-Gyu 대한이식학회 2021 Korean Journal of Transplantation Vol.35 No.2

Salvage liver transplantation (LT) is a definite treatment for recurrent hepatocellular carcinoma (HCC) after hepatectomy. Combined hepatocellular carcinoma-cholangiocarcinoma (cHCC-CC) is not eligibly indicated for LT because of high post-transplant recurrence. We present a case of salvage living donor liver transplantation (LDLT) in a patient with tumor recurrence after surgical resection of cHCC-CC. A 61-year-old male patient diagnosed with chronic hepatitis B underwent right posterior sectionectomy for HCC. The pathological diagnosis revealed presence of a 3.2-cm-sized cHCC-CC with stem cell features and intermediate cell-subtype. Intrahepatic tumor recurrence occurred 9 months later and transarterial chemoembolization was performed. Due to the progress of recurrent tumors, ABO-incompatible LDLT was performed. The explant liver pathology revealed four small masses of cHCC-CC with stem cell features. Pulmonary metastasectomy and chest wall resection were performed for metastatic lesions at 10 months after LT. Multiple tumor recurrence was detected at 21 months after LT with progression despite sorafenib administration. Currently, the patient is alive for past 26 months after LT. In conclusion, the clinical sequences of this case suggest that the role of salvage LT for cHCC-CC is much more limited than that for HCC because the tumor biology of cHCC-CC is more aggressive compared with HCC.

Behaviour of a column–bent cap connection for a precast DSCT column

Won, Deok Hee,Kim, Seungjun,Seo, Jihye,Lim, Jung Hyun,Kang, Young Jong Thomas Telford Ltd. 2018 Magazine of concrete research Vol.70 No.2

Establishment and evaluation of immortalized human epidermal keratinocytes for an alternative skin irritation test

Kim, Cho-Won,Kim, Chang Deok,Choi, Kyung-Chul Elsevier 2017 Journal of pharmacological and toxicological metho Vol.88 No.2

<P><B>Abstract</B></P> <P>Human skin is located at the outermost part of the body, and various cosmetics and chemicals that may come in contact with human skin need to be evaluated for their potential to cause irritation. Until recently, the Draize test was considered the standard method for skin irritation; however, this technique has disadvantages such as the need to sacrifice many rabbits and subjective scoring. Thus, to contribute to the development of an animal-free alternative skin irritation test, we investigated the cytotoxicity and inflammatory response to standard skin irritants in SV40 large T antigen-transformed human epidermal keratinocyte 2 cells (SV-HEK2 cells). In this study, we established an SV-HEK2 cell line immortalized by SV40 large T antigen (SV40 T) and characterized the inherent morphological and cytological properties. We next used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) or neutral red uptake (NRU) assays of cell viability to investigate the optimal experimental conditions for determining SV-HEK2 cell viability after exposure to sodium dodecyl sulfate at 6.25×10<SUP>−3</SUP>% to 1×10<SUP>−1</SUP>% as a standard skin irritant. We then examined the viability of SV-HEK2 cells in response to five skin irritants (benzalkonium chloride, isopropanol, sodium dodecyl sulfate, Triton X-100 and Tween20) at 5×10<SUP>−3</SUP>% to 1×10<SUP>−1</SUP>% by MTT or NRU assay. Finally, we estimated the level of cytokines secretion in response to stimulation by skin irritants in SV-HEK2 cells. The results revealed that SV-HEK2 cells responded well to skin irritants in a concentration-dependent manner and that there was good correlation between irritant concentration and cytotoxicity (or cytokine secretion) when cells were exposed to skin irritants for 10min at room temperature (RT) using an MTT assay. Overall, these findings suggest that SV-HEK2 cells could be a good alternative <I>in vitro</I> model for skin irritation tests.</P>

Detection of physiological events by impedance

Kim, Deok-Won Yonsei University, College of Medicine 1989 Yonsei medical journal Vol.30 No.1

A Case of Successful Recovery from High Dose Intravenous Nicorandil Infusion in Refractory Coronary Vasospasm with Hemodynamic Collapse

Won-Jun Koh,Jeong-Hyeon Cho,Ji-Hyun Lee,Won-Sik Kang,Min-Kyung Lee,Jun-Hyoung Kim,Deok-kyu Cho 영남대학교 의과대학 2012 Yeungnam University Journal of Medicine Vol.29 No.2

A 70-year-old male came to the emergency room of the authors’ hospital because of sudden cardiac arrest due to inferior wall ST elevation myocardial infarction. His coronary angiography revealed multiple severe coronary spasms in his very long left anterior descending artery. After an injection of intracoronary nitroglycerine, his stenosis improved. The cardiac arrest relapsed, however, accompanied by ST elevation of the inferior leads, while the patient was on diltiazem and nitrate medication to prevent coronary spasm. Recovery was not achieved even with cardiac massage, intravenous injection of epinephrine and atropine, and intravenous infusion of nitroglycerine. The patient eventually recovered through high-dose nicorandil intravenous infusion without ST elevation of his inferior leads. Therefore, intravenous infusion of a high dose of nicorandil must be considered a treatment option for cardiac arrest caused by refractory coronary vasospasm.

산화물 음극 표면의 과잉 바륨 형성 메커니즘 규명

Won, Byeong-Muk,Park, Gong-Seok,Hwang, Cheol-Ho,Han, Su-Deok,Kim, In-U,Gwon, Yong-Beom,Seol, Seung-Gwon,Jo, Chang-Sik,Je, Jeong-Ho,Hwu, Y.,Tsai, W.L.,Ruterana, P. 한국정보디스플레이학회 2003 인포메이션 디스플레이 Vol.4 No.4

우리는 연-X-선 흡수 분광법(soft-X-ray Absorption Spectroscopy ; XAS)을 사용하여 실제 산화물 음극 전자 방출 물질 표면의 화학 구조에 대해 연구하였다. 바륨 3d 흡수단의 고-에너지 분해 스펙트럼은 활성화 과정(온도와 전압 인가) 후에 산화물 음극표면에서 수십 나노미터 깊이 아래까지 바륨 함량이 현저히 증가한다는 것을 보여주었다. 바륨과 산소의 XAS 데이터를 비교해보면 표면에서 과잉 바륨이 형성되는 것을 확실히 입증하고 있다. 게다가 우리는 표면에서의 과잉 바륨의 증가가 활성화 과정에서 열에너지에 의한 것이 아니라 인가된 전압에 의한 것임을 밝혀냈다. 이 결과로 우리는 표면의 과잉 바륨 형성 전체 과정을 규명하고 활성화 동안 표면의 바륨 증가가 분말 입자로부터 니켈 계면으로의 바륨 이온의 전해 이동 속도에 의해 제어된다는 것을 제안한다. 우리는 또한 표면 바륨 증가가 전해 이동에 의한 분말 입자 내의 바륨의 고갈에서 비롯되는 것으로 추정한다.

Improvement of xylose fermentation by a mutant Kluyveromyces marxianus through a laboratory evolutionary approach and random mutations

Deok-Ho KWON,Jin-Seong KIM,Jae-Bum PARK,Seung-Won JANG,Suk-Jin HA 한국생물공학회 2014 한국생물공학회 학술대회 Vol.2014 No.10