G-3. Breeding of the Gad-mdx Mouse

Jun Gyo Suh,Akerni Yamazaki,Takeshi Tomita 한국실험동물학회 1993 한국실험동물학회 학술발표대회 논문집 Vol.1993 No.-

Chelate Effects in Glyme/Lithium Bis(trifluoromethanesulfonyl)amide Solvate Ionic Liquids, Part 2: Importance of Solvate-Structure Stability for Electrolytes of Lithium Batteries

Zhang, Ce,Yamazaki, Azusa,Murai, Junichi,Park, Jun-Woo,Mandai, Toshihiko,Ueno, Kazuhide,Dokko, Kaoru,Watanabe, Masayoshi American Chemical Society 2014 The Journal of Physical Chemistry Part C Vol.118 No.31

<P>Highly concentrated, molten mixtures of lithium bis(trifluoromethanesulfonyl)amide (Li[TFSA]) and ether solvents (tetrahydrofuran (THF), monoglyme (G1), diglyme (G2), and triglyme (G3)) were investigated as electrolytes for Li batteries. To compare the electrochemical reactions in the electrolytes with different solvents, the ratio of ether–oxygen atoms and Li<SUP>+</SUP> ([O]/[Li]) in the electrolytes was fixed at four. The capacity of a Li–LiCoO<SUB>2</SUB> cell with [Li(THF)<SUB>4</SUB>][TFSA] dramatically decreased upon charge/discharge cycling, whereas [Li(G3)<SUB>1</SUB>][TFSA] allowed the cell to have a stable charge–discharge cycles and a Coulombic efficiency of greater than 99% over 100 cycles. Corrosion of the Al current collector of the cathode was also affected by the composition of the electrolytes. Persistent Al corrosion took place in [Li(THF)<SUB>4</SUB>][TFSA] and [Li(G1)<SUB>2</SUB>][TFSA], which contain shorter ethers, but the corrosion was effectively suppressed in [Li(G3)<SUB>1</SUB>][TFSA]. Furthermore, lithium polysulfides, which are formed as discharge intermediates at the sulfur cathode of the Li–S cell, were much less soluble in electrolytes with longer ethers. Therefore, a higher Coulombic efficiency and more stable cycle ability were achieved in Li–S cells with [Li(G3)<SUB>1</SUB>][TFSA]. All the electrochemical properties in the batteries were dominated by the presence or absence of uncoordinating solvents in the concentrated electrolytes. This paper demonstrates that the structural stability of [Li(glyme or THF)<SUB><I>x</I></SUB>]<SUP>+</SUP> cations in electrolytes plays an important role in the performance of Li batteries.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2014/jpccck.2014.118.issue-31/jp504099q/production/images/medium/jp-2014-04099q_0010.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp504099q'>ACS Electronic Supporting Info</A></P>

Emerging evidence for crosstalk between Nrf3 and mitochondria in physiological homeostasis and in heart disease

Michiko Tsushima,Jun Liu,Wataru Hirao,Hiromi Yamazaki,Hirofumi Tomita,Ken Itoh 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.3

Nrf2 regulates redox homeostasis in cells bycoordinately regulating a range of antioxidant enzymes andproteins. An increase in oxidative stress is one of the hallmarksof aging, and Nrf2 protein levels and activity decreasewith aging. Decreased mitochondrial functions, such asdecreased ATP production, also occur with aging, leadingto the increased generation of reactive oxygen species (ROS)and oxidative stress. Thus, understanding the relationshipsbetween Nrf2 and the mitochondria is important for clarifyingthe regulatory mechanisms of aging. It is becomingclear that Nrf2 is activated in a tissue-specific manner inresponse to mitochondrial or NADPH oxidase-generatedROS. As the heart consists of postmitotic cells that utilizeATP produced mainly by mitochondrial oxidative phosphorylation,cardiomyocytes are equipped with highly sophisticatedmitochondrial quality control mechanisms. Consistentwith these findings, it has been reported that Nrf2 inthe heart is regulated via a specific translational mechanismand that Nrf2 activation confers cardioprotective effects invarious disease models. Thus, Nrf2 is a promising target foranti-aging strategies to combat age-related heart diseases,such as age-related cardiomyopathy.

Improved forecasts of winter weather extremes over midlatitudes with extra Arctic observations : ROLE OF ARCTIC OBSERVATIONS ON FORECASTS

Sato, Kazutoshi,Inoue, Jun,Yamazaki, Akira,Kim, Joo-Hong,Maturilli, Marion,Dethloff, Klaus,Hudson, Stephen R.,Granskog, Mats A. American Geophysical Union 2017 Journal of geophysical research. Oceans Vol.122 No.2

Anionic Effects on Solvate Ionic Liquid Electrolytes in Rechargeable Lithium–Sulfur Batteries

Ueno, Kazuhide,Park, Jun-Woo,Yamazaki, Azusa,Mandai, Toshihiko,Tachikawa, Naoki,Dokko, Kaoru,Watanabe, Masayoshi American Chemical Society 2013 JOURNAL OF PHYSICAL CHEMISTRY C - Vol.117 No.40

<P>A series of equimolar mixtures of Li salts (LiX) and glymes (triglyme (G3) and tetraglyme (G4)), [Li(glyme)]X with different anions (X: [N(SO<SUB>2</SUB>C<SUB>2</SUB>F<SUB>5</SUB>)<SUB>2</SUB>] = [BETI]; [N(SO<SUB>2</SUB>CF<SUB>3</SUB>)<SUB>2</SUB>] = [TFSA]; [CF<SUB>3</SUB>SO<SUB>3</SUB>] = [OTf]; BF<SUB>4</SUB>; NO<SUB>3</SUB>), were used as electrolytes to study the anionic effects of [Li(glyme)]X on the performance of lithium–sulfur (Li–S) batteries. The dissolution of lithium polysulfides (Li<SUB>2</SUB>S<SUB><I>m</I></SUB>), which are discharge products of elemental sulfur, was significantly suppressed in the solvate ionic liquid (IL) electrolytes, as seen in [Li(G4)][BETI] and [Li(glyme)][TFSA], wherein all of the glymes participated in the formation of the complex cation [Li(glyme)]<SUP>+</SUP>. It was found that NO<SUB>3</SUB> anions were irreversibly reduced at the composite cathode during discharge and BF<SUB>4</SUB> anions formed unexpected byproducts through a chemical reaction with the polysulfide anions. Successful charge/discharge of Li–S cell could not be performed in [Li(glyme)]X in the presence of these anions because of the undesired side reactions. The solvate IL [Li(G4)][BETI] was found to be electrochemically stable in the Li–S cell and allowed a stable operation with a capacity of 600–700 mAh·g<SUP>–1</SUP> and a Coulombic efficiency of 98.5% over 100 cycles, similar to that achieved by [Li(glyme)][TFSA]. In contrast, the Li–S cell with a concentrated electrolyte solution, [Li(G3)][OTf], showed a much lower capacity and Coulombic efficiency.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jpccck/2013/jpccck.2013.117.issue-40/jp407158y/production/images/medium/jp-2013-07158y_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jp407158y'>ACS Electronic Supporting Info</A></P>

Social work for vulnerable and marginalized people during COVID‐19 in Japan

Murayama Yoh,Hasebe Masami,Yamazaki Sachiko,Yamaguchi Jun,Kobayashi Erika 한국사회복지학회 2023 Asian Social Work and Policy Review Vol.17 No.3

During the Coronavirus disease pandemic (COVID‐19), working conditions became increasingly difficult for social workers, as they had to deal with new challenges and added workloads. This study describes the difficulties faced by social workers of the Self‐Reliance Support System in Japan and clarifies the solution for each difficulty while conducting their duties to support vulnerable people during COVID‐19. To this end, we conducted an open‐ended questionnaire survey and an interview survey. The data were analyzed using qualitative coding. The results showed that the difficulties faced in consultation support work included supporting hitherto socially invisible groups such as sole traders and foreigners. Social workers also faced an ethical dilemma, as they could not provide support to clients who “did not try to solve their problems” or for whom “interventional attempts did not lead to counseling support” and who only sought benefits at the risk of becoming isolated and impoverished. Following this, consultants collaborated to resolve such difficulties while maximizing the use of various local resources. The study's findings clarify that it would be effective to understand the difficulties faced by social workers to obtain hints toward their resolution.

Solvate Ionic Liquid Electrolyte for Li–S Batteries

Dokko, Kaoru,Tachikawa, Naoki,Yamauchi, Kento,Tsuchiya, Mizuho,Yamazaki, Azusa,Takashima, Eriko,Park, Jun-Woo,Ueno, Kazuhide,Seki, Shiro,Serizawa, Nobuyuki,Watanabe, Masayoshi The Electrochemical Society 2013 Journal of the Electrochemical Society Vol.160 No.8

<P>Innovation in the design of electrolyte materials is crucial for realizing next-generation electrochemical energy storage devices such as Li–S batteries. The theoretical capacity of the S cathode is 10 times higher than that of conventional cathode materials used in current Li–ion batteries. However, Li–S batteries suffer from the dissolution of lithium polysulfides, which are formed by the redox reaction at the S cathode. Herein, we present simple solvate ionic liquids, glyme–Li salt molten complexes, as excellent electrolyte candidates because they greatly suppress the dissolution of lithium polysulfides. The molten complexes do not readily dissolve other ionic solutes, which leads to the stable operation of the Li–S battery over more than 400 cycles with discharge capacities higher than 700 mAh g-sulfur<SUP>−1</SUP> and with coulombic efficiencies higher than 98% throughout the cycles. Such high performance has not been realized to the best of our knowledge. Furthermore, the addition of a nonflammable fluorinated solvent, which does not break the solvate structure of the glyme–Li salt molten complexes, greatly enhances the power density of the Li–S battery. The strategic design of electrolyte properties provides opportunities for the development of new electrochemical devices with many different electrode materials.</P>

Differentiation of Rat Dermal Mesenchymal Cells and Calcification in Three-Dimensional Cultures

Taiki Suyama,Mitsutoki Hatta,Shozaburo Hata,Hiroyuki Ishikawa,Jun Yamazaki 한국조직공학과 재생의학회 2016 조직공학과 재생의학 Vol.13 No.5

Three-dimensional (3D) cultures are known to promote cell differentiation. Previously, we investigated the differentiation of rat dermal fibroblasts to α-smooth muscle actin (α-SMA)-positive myofibroblasts through transforming growth factor (TGF)-β production using a 3D culture model. Here, we investigated the phenotypic change from dermal mesenchymal cells (mostly fibroblasts) to osteoblast-like cells, being inspired by the roles of smooth muscle cells or fibroblasts during vascular calcification. Spindle-shaped cells that grew in heterologous populations out of dermal explants from 2-day-old Wistar rats were cultured within a collagen matrix. α-SMA and alkaline phosphatase (ALP) messenger RNA (mRNA) levels initially increased, followed by a rise in Runx2 and osteocalcin (OCN) mRNA levels without calcification. Calcium deposits were produced in the presence of a high concentration of inorganic phosphate (2.1 mM) or β-glycerophosphate (βGP, 10 mM) after 2 weeks of culture, and both were sensitive to an inhibitor of type III phosphate transporters. An ALP inhibitor decreased only βGP-induced calcification. Inhibition of TGF-β type-I receptors attenuated ALP mRNA levels and βGP-induced calcification, suggesting that endogenous TGF-β stimulates ALP activity and then βGP breakdown. An increase in the number of cells embedded in the collagen gel enhanced the mRNA levels of Runx2 and OCN, but not of ALP. Collectively, several factors are likely to promote the differentiation of dermal mesenchymal cells into osteoblast-like cells and ectopic calcification in a 3D collagen matrix, implying the utility of these cells as a potential autologous cell source for tissue engineering.

Rapid Changes in Serum Lipid Profiles during Combination Therapy with Daclatasvir and Asunaprevir in Patients Infected with Hepatitis C Virus Genotype 1b

Takeshi Chida,Kazuhito Kawata,Kazuyoshi Ohta,Erika Matsunaga,Jun Ito,Shin Shimoyama,Satoru Yamazaki,Hidenao Noritake,Tetsuro Suzuki,Takafumi Suda,Yoshimasa Kobayashi 거트앤리버 소화기연관학회협의회 2018 Gut and Liver Vol.12 No.2

Background/Aims: Changes in lipid profiles in patients infected with hepatitis C virus (HCV) during direct-acting antiviral therapy have been reported in recent years. However, the clinical aspects of disturbed lipid metabolism in chronic HCV infection have not been fully elucidated. Methods: Dynamic changes in serum total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol and apolipoprotein levels in patients infected with HCV genotype 1b were examined during combination therapy with daclatasvir (DCV) and asunaprevir (ASV). Results: Total, LDL-, and HDL-cholesterol levels increased rapidly and persistently after week 4. Apolipoprotein (apo) A-I, apo B, apo C-II, and apo C-III levels were significantly higher at week 4 than at week 0. In contrast, apo A-II and apo E levels were significantly lower. The differences in LDL- and HDL-cholesterol levels were positively correlated with those of apo B and apo A-I, respectively. Interestingly, in patients with non-sustained virological response, these cholesterol levels decreased rapidly after viral breakthrough or viral relapse. Furthermore, similar changes were observed for apo A-I, apo B and apo C-III levels. Conclusions: Clearance of HCV using combination therapy with DCV and ASV results in rapid changes in serum lipid profiles, suggesting an influence of HCV infection on disturbed lipid metabolism.