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Masahide Yasuda,Yasuyuki Ishii,Kazuyoshi Ohta 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
Recently Napier grass (Pennisetum purpureumSchumach) has been recognized to meet the requirement oflignocellulosic bioethanol production, because it has lowlignin-content and a relatively high herbage mass per yearand per area. Therefore, pretreatment, saccharification, andfermentation processes for ethanol production from Napiergrass have been extensively studied. As pretreatmentmethod, acid, alkali, PBHW (pressurized batch hot water),and LMAA (low-moisture anhydrous ammonia) pretreatmentswere reviewed. As saccharification and fermentationprocess, saccharification followed by co-fermentation ofhexose and pentose, simultaneous saccharification andfermentation (SSF) followed by pentose fermentation,simultaneous saccharification and co-fermentation (SSCF)process were proposed. The SSCF was most advantageousprocess since the SSCF can prevent contamination risks ofother microorganism and can construct simple processingprocedure. An example of ethanol production from Napiergrass was a combination process of LMAA-pretreatmentwith SSCF which was performed for of LMAA-treatedNapier grass at 36°C for 96 h using cellulase, xylanase,Saccharomyces cerevisiae, and Escherichia coli KO11. The ethanol yield reached 74.1%. Thus, Napier grass wasthought to be a promising biomass for ethanol production.
Takeshi Chida,Kazuhito Kawata,Kazuyoshi Ohta,Erika Matsunaga,Jun Ito,Shin Shimoyama,Satoru Yamazaki,Hidenao Noritake,Tetsuro Suzuki,Takafumi Suda,Yoshimasa Kobayashi 거트앤리버 소화기연관학회협의회 2018 Gut and Liver Vol.12 No.2
Background/Aims: Changes in lipid profiles in patients infected with hepatitis C virus (HCV) during direct-acting antiviral therapy have been reported in recent years. However, the clinical aspects of disturbed lipid metabolism in chronic HCV infection have not been fully elucidated. Methods: Dynamic changes in serum total, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) cholesterol and apolipoprotein levels in patients infected with HCV genotype 1b were examined during combination therapy with daclatasvir (DCV) and asunaprevir (ASV). Results: Total, LDL-, and HDL-cholesterol levels increased rapidly and persistently after week 4. Apolipoprotein (apo) A-I, apo B, apo C-II, and apo C-III levels were significantly higher at week 4 than at week 0. In contrast, apo A-II and apo E levels were significantly lower. The differences in LDL- and HDL-cholesterol levels were positively correlated with those of apo B and apo A-I, respectively. Interestingly, in patients with non-sustained virological response, these cholesterol levels decreased rapidly after viral breakthrough or viral relapse. Furthermore, similar changes were observed for apo A-I, apo B and apo C-III levels. Conclusions: Clearance of HCV using combination therapy with DCV and ASV results in rapid changes in serum lipid profiles, suggesting an influence of HCV infection on disturbed lipid metabolism.