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John Jeongseok Yang,조선영,Jin-Tae Suh,이희주,이우인,윤휘중,백선경,박태성 대한진단검사의학회 2012 Annals of Laboratory Medicine Vol.32 No.5
An 87-yr-old woman was diagnosed with AML with myelodysplasia-related changes (AMLMRC).The initial complete blood count showed Hb level of 5.9 g/dL, platelet counts of 27×109/L, and white blood cell counts of 85.33×109/L with 55% blasts. Peripheral blood samples were used in all the tests, as bone marrow examination could not be performed because of the patient’s extremely advanced age and poor general health condition. Flow cytometric analysis, chromosome analysis, FISH, and reverse transcriptase-PCR (RT-PCR) results indicated AML-MRC resulting from t(3;21) with the RUNX1-MECOM fusion gene. To our knowledge, this is the second most elderly de novo AML patient associated with t(3;21) to be reported.
Evaluation of a Chromogenic Culture Medium for the Detection of Clostridium difficile
John Jeongseok Yang,이희주,남유선,김민진,조선영,유은경,소윤수 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.4
Purpose: Clostridium difficile (C. difficile) is an important cause of nosocomial diarrhea. Diagnostic methods for detection of C. difficile infection (CDI) are shifting to molecular techniques, which are faster and more sensitive than conventional methods. Although recent advances in these methods have been made in terms of their cost-benefit, ease of use, and turnaround time, anaerobic culture remains an important method for detection of CDI. Materials and Methods: In efforts to evaluatea novel chromogenic medium for the detection of C. difficile (chromID CD agar), 289 fecal specimens were analyzed using two other culture media of blood agar and cycloserine-cefoxitin-fructose-egg yolk agar while enzyme immunosorbentassay and polymerase chain reaction-based assay were used for toxin detection. Results: ChromID showed the highest detection rate among the three culture media. Both positive rate and sensitivity were higher from chromID than other culturemedia. ChromID was better at detecting toxin producing C. difficile at 24 h and showed the highest detection rate at both 24 h and 48 h. Conclusion: Simultaneous use of toxin assay and anaerobic culture has been considered as the most accurate and sensitive diagnostic approach of CDI. Utilization of a more rapid and sensitive chromogenic medium will aid in the dianogsis of CDI.
Yang, John Jeongseok,Park, Tae Sung,Choi, Jong Rak,Park, Seo-Jin,Cho, Sun Young,Jun, Kyung Ran,Kim, Hye Ran,Lee, Jeong Nyeo,Oh, Seung Hwan,Lee, Sanggyu,Kim, Bomi,Marschalek, Rolf,Meyer, Claus S. Karger AG 2012 Acta haematologica Vol.127 No.2
<P>Abstract</P><P>No abstract available</P><P>Copyright © 2010 S. Karger AG, Basel</P>
John Jeongseok Yang,Jin Seok Kim,Yousun Chung,Hyungsuk Kim,고대현,Sang-Hyun Hwang,Heung-Bum Oh 대한임상검사정도관리협회 2021 Journal of Laboratory Medicine And Quality Assuran Vol.43 No.1
Background: This study summarizes a survey conducted to improve and expand the external quality assessment (EQA) scheme for transfusion in Korea. Methods: The EQA items provided by the College of American Pathologists, Royal College of Pathologists of Australasia, National External Quality Assessment Service, and German program were explored. A commercially operated EQA program was also included in the survey. Results: Items not included in the Korean EQA programs included extended antigen typing, electronic cross-matching, eluate survey, and fetal red cell detection. Granulocyte- and platelet-related testing was also not covered by the Korean program. There were issues regarding establishing the criteria for acceptable ranges and manufacturing of EQA materials. Conclusions: We successfully conducted a survey for the improvement and expansion of EQA for transfusion medicine. A pilot project for extended antigen typing is expected to be implemented in 2021, and ongoing expansion will continue in the following years.
John Jeongseok Yang,Jin Seok Kim,Young Ae Lim,Hyungsuk Kim,Dae-Hyun Ko 대한임상검사정도관리협회 2020 Journal of Laboratory Medicine And Quality Assuran Vol.42 No.3
This report summarizes the 2019 survey results of the external quality assessment (EQA) scheme for the Transfusion Medicine Program (TMP) in Korea. Proficiency testing materials were prepared at the Asan Medical Center for the biannual distribution to participating laboratories. The average accuracy rates and number of participants (in parenthesis) for ten survey items were as follows: ABO typing, 99.4%–99.9% (N=875); RhD typing, 99.8%–100% (N=864); crossmatching, 90.8%–99.6% (N=760); ABO subtyping, 98.2% and 100% (N=58); Rh CcEe antigen testing, both 100% (N=55); weak D test, 97.9%–100% (N=232); antibody screening, 99.7%– 100% (N=316); direct antiglobulin test (DAT) using a polyspecific reagent, 99.6%–100% (N=273); DAT using an immunoglobulin-G monospecific reagent, both 100.0% (N=67); DAT using a C3d monospecific reagent, 95.6%–98.5% (N=67); antibody identification, 87.9%–99.2% (N=132); and ABO Ab titration, 85.7%–100% (N=134). The number of participants showed an average increase of 14% across the ten survey items, with the ABO antibody titration showing the highest increase at 83.6%. While results were generally excellent, antibody identification and ABO antibody titration results showed room for improvement. The 2019 EQA scheme for TMP has contributed to the improvement and maintenance of the participating laboratories to the program.
Prediction of HLA-DQ in Deceased Donors and its Clinical Significance in Kidney Transplantation
Kim Soo-Kyung,Yang John Jeongseok,Hwang Sang-Hyun,Sung Heungsup,Shin Sung,Ko Sun-Young,Oh Heung-Bum 대한진단검사의학회 2021 Annals of Laboratory Medicine Vol.41 No.2
Background: HLA-DQ typing in deceased donors is not mandatory in Korea. Therefore, when patients develop DQ antibodies after kidney transplantation (KT) from deceased donor, it is impossible to determine whether they are donor-specific antibodies (DSA). We developed DQ prediction programs for the HLA gene and evaluated their clinical utility. Methods: Two HLA-DQ prediction programs were developed: one based on Lewontin’s linkage disequilibrium (LD) and haplotype frequency and the other on an artificial neural network (ANN). Low-resolution HLA-A, -B, -DR, and -DQ typing data of 5,603 Korean patients were analyzed in terms of haplotype frequency and used to develop an ANN DQ prediction program. Predicted DQ (pDQ) genotype accuracy was analyzed using the typed DQ data of 403 patients. pDQ DSA agreement, sensitivity, specificity, and false-negative rate was evaluated using 1,970 single-antigen bead assays performed on 885 KT recipients. The clinical significance of DQ and pDQ DSA was evaluated in 411 KT recipients. Results: pDQ genotype accuracies were 75.4% (LD algorithm) and 75.7% (ANN). When the second most likely pDQ (LD algorithm) was also considered, the genotype accuracy increased to 92.6%. pDQ DSA (LD algorithm) agreement, sensitivity, specificity, and false-negative rate were 97.5%, 97.3%, 98.6%, and 2.4%, respectively. The antibody-mediated rejection treatment frequency was significantly higher in DQ or pDQ DSA-positive patients than in DQ or pDQ DSA-negative patients (P<0.001). Conclusions: Our DQ prediction programs showed good accuracy and could aid DQ DSA detection in patients who had undergone deceased donor KT without donor HLA-DQ typing.