벼의 dihydrodipicolinate synthase 유전자의 cloning 및 분석

서미정,조수진,임용표 한국육종학회 1996 한국육종학회 학술발표회 발표요지 Vol.28 No.1

유아기 기질의 종단적 프로파일과 학령 초기 학교 적응

서미정,공유경 한국지역사회생활과학회 2022 한국지역사회생활과학회지 Vol.33 No.3

This study identifies patterns of change in temperament among infants aged 2 to 4 years old, and investigates the relationships between such patterns and school adjustments in the first grade of elementary school. Five distinct latent classes for patterns of change in children’s temperament emerged through latent profile analysis using three-year data from the Panel Study on Korean Children (N = 1,869): a) inactive & shy child (13.5%), b) decreasing activity & mild child (44.0%), c) highly emotional & unsociable child (13.4%), d) decreasing emotionality & highly sociable child (14.7%), and e) emotional & sociable child (14.4%). Significant associations with the five profiles of temperament were found for both gender and birth order of the children. Only the inactive & shy child class had more girls than boys; 47.1% of the children in the highly emotional & unsociable child class were the second child, while all other classes had more first children. Each child in the inactive & shy child class had a higher level of adjustment to school life than those in the highly emotional & unsociable child and the decreasing emotionality & highly sociable child classes when the children were seven years old. As for academic achievement, children in the decreasing activity & mild child class, in the decreasing emotionality & highly sociable child, and in the emotional & sociable child class exhibited more adjustments than those in the highly emotional & unsociable child class. Among the sub-domains of school adjustment, peer adjustment and teacher adjustment for the first grade of elementary school did not have significant associations with longitudinal profiles of temperament. The findings indicate that temperament through early childhood predicts adjustment to school life and academic achievement at an early school age.

Daisdzein이 Benzo(k)fluoranthene에 의한 CYP1B1 유전자조절 작용에 미치는 영향

서미정,김여운,신윤용,Seo, Mi-Jeong,Kim, Yeo-Woon,Sheen, Yhun-Yhong 한국환경성돌연변이발암원학회 2004 한국환경성돌연변이·발암원학회지 Vol.24 No.4

Cytochrome P4501B1(CYP1B1) is known to be inducible by xenobiotic compounds such as policyclic aromatic hydrocarbon(PAH) and dioxins such as 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). And these induction of CYP1B1 is also regulated by many categories of chemicals. In order to investigate the effects of several chemicals on CYP1B1 gene expression in Hepa-I and MCF-7 cells, 5' flanking DNA of human CYP1B1 was cloned into pGL3 basic vector containing luciferase gene, and then transfected into these cells. After treatment of chemicals, the luciferase activity was measured. CYP1B1 enzyme metabolize PAHs and estradiol. CYP1B1 metabolize estradiol to 4-hydrozyestradiol that is considered as carcinogenic metabolite. Recent industrialized industrialized society, human has been widely been exposed to widespread environmental contaminants such as PAHs(polycyclic aromatic hydrocarbon) that are originated from the imcomplete combustion of hydrocarbons. PAHs are known to be ligands of the AhR(aryl hydrocarbon receptor). Induction of cytochrome P4501B1(CYP1B1) in cell culture is widely used as a biomarker for PAHs. Therefore we have studied the effect of PAHs in the human breast cancer cells MCF-7 to evaluate bioactivity of PAHs. We have used the United State of America EPA selected 13 different PAHs, PAHs mixtures and extracts from environmental samples to evaluate the bioassay system. We examined effects of PAHs on the CYP1B1-luciferase reporter gene and CYP1B1 mRNA level. Benzo(k)fluoranthene and dibenzo(a, h)anthracene showed strong response to CYP1B1 promoter activity stimulation, and also CYP1B1 mRNAs increase in MCF-7 cells in a concentration-dependent manner. RT-PCR analysis indicated that PAHs significantly up-regulate the level of CYP1B1 mRNA. Some flavonoids such as genistein, daidzein, chrysin, naringenin and morin were also investigeted. These flavonoids decreased B(k)F infuced luciferase activity at low concentration. But, these flavonoids exhibited stimulatory effect at high concentration.

첫 자녀 출산 부모의 결혼만족도 변화경로 및 예측요인

서미정 육아정책연구소 2018 육아정책연구 Vol.12 No.3

본 연구는 첫 자녀 출생 후부터 영유아기 동안 아버지와 어머니의 결혼만족도 변화경로를 탐색하고, 이에 대한 아버지 양육참여 및 부부갈등의 영향력을 살펴보았다. 자료는 한국아동패널의 5개년 자료(1차년도~5차년도)를 활용하였고, 최종 표본은 첫 자녀를 출산한 984가구이다. 먼저 잠재성장모형 분석을 통해 아버지와 어머니의 결혼만족도는 시간 흐름에 따라 감소하는 변화패턴이 확인되었다. 하지만 결혼만족도는 아버지가 어머니에 비해 더욱 가파르게 감소하였고, 모든 측정 시점에서 아버지보다 어머니가 낮은 수준을 나타내었다. 다음으로 아버지의 결혼만족도 변화에 대한 영향요인은 통제변수인 어머니의 취업만이 유의미하게 나타났다. 이에 반해 어머니의 결혼만족도 변화에는 첫 자녀 출산 후 아버지의 양육참여 및 부부갈등이 영향을 미치는 것으로 나타났다. 본 연구결과는 영유아기 자녀를 양육하는 동안 결혼만족도 향상을 위해서는 아버지와 어머니 간에 차별적 개입이 필요함을 시사하였다. The aim of this study was to examine trajectories in and predictors of marital satisfaction among parents with one year old baby. Latent growth modeling was used with a sample of 984 households. Data from Panel Study on Korean Children conducted by Korea Institute of Child Care and Education were used. The study results revealed that for both fathers and mothers, marital satisfaction decreased over time after the birth of the first child. However, fathers’ marital satisfaction decreased more steeply than mothers’. Furthermore, mothers’ employment was associated with the changes of fathers’ marital satisfaction. Father involvement and marital conflict predicted changes of mothers’ marital satisfaction. These findings show that factors such as father involvement and marital conflict are more highly associated with changes in mothers’ marital satisfaction than fathers’. Also, the study suggests that parent education should be systematically organized to promote father involvement and to resolve marital conflict.

또래괴롭힘 참여자 역할의 안정성과 주변또래 역할의 변화와 관련된 부모, 또래, 교사 및 지역사회 요인

서미정 가정과삶의질학회 2015 가정과삶의질학회 학술발표대회 자료집 Vol.2015 No.5

또래괴롭힘에서 아동의 성별에 따른 주변또래행동의 변화양상

서미정 가정과삶의질학회 2017 가정과삶의질학회 학술발표대회 자료집 Vol.2017 No.5

아동과 청소년의 완벽주의 관련 연구 동향

서미정,박서연 가정과삶의질학회 2017 가정과삶의질학회 학술발표대회 자료집 Vol.2017 No.11

부모의 심리사회적 특성이 후속 출산계획에 미치는 영향 : 유자녀 가구를 중심으로

서미정 육아정책연구소 2011 육아정책연구 Vol.5 No.1

본 연구는 한국아동패널의 1차년도 자료를 활용하여 유자녀 가구의 후속 출산계획과 관련된 부모의 심리사회적 특성을 규명하고, 추가 출산하지 않는 이유를 파악하였다. 주요 연구결과는 다음과 같다. 첫째, 후속 출산계획에 대해 자녀수 및 모 연령이 적을수록 ‘낳지 않겠다’는 응답율은 낮은 반면, ‘낳겠다’와 ‘모르겠다’는 높은 응답율을 보여주었다. 둘째, 자녀수 및 모 연령이 적을수록, 자녀의 미래에 대한 기대가 높을수록, 아버지의 양육참여도가 높을수록, 그리고 모의 우울 수준이 낮을수록 후속 출산에 대한 무계획집단에 비해 계획집단에 속할 가능성이 높게 나타났다. 후속 출산에 대한 계획집단과 미결정집단을 구분하는 데에는 자녀수, 모 연령, 모 학력, 모 우울이 유의미한 영향력을 나타내었다. 셋째, 자녀출산 가능성이 비교적 높아 보이는 자녀수가 1명이거나 연령이 30세 미만인 여성은 추가 출산하지 않는 이유로 자녀교육비용과 출산 및 양육의 어려움을 우선적으로 꼽고 있었다. 이러한 결과를 근거로 향후 출산장려정책에 부모의 심리사회적 특성이 반영될 필요성이 논의되었다. This study aimed to identify parents' psychosocial characteristics affect follow-up planned childbirth among family with children from the Panel Study of Korean Children. Findings from this study are as follows. First, follow-up planned childbirth was found significant difference by both the number of children and female age. Second, logistic regression analysis shows that expectations for a child's future, father's involvement for parental role, and postpartum depression were all strongly associated with follow-up planned childbirth. Third, the reasons why women with children don't plan a follow-up childbirth was that education expenditure for children, followed by caring difficulties among women with one child and under 29 year-old-women. These findings suggest that parents' psychosocial characteristics should be reflected on birth encouragement policy to increase childbirth.

최백호 : 나이듦에 관하여

서미정 샘터사 2015 월간 샘터 Vol.550 No.-

초기 청소년의 휴대전화 용도별 이용빈도의 변화가 심리사회적 부적응에 미치는 영향

서미정 가정과삶의질학회 2016 가정과삶의질학회 학술발표대회 자료집 Vol.2016 No.5