Cyclosporin A 로 유도된 생쥐 림프절의 세포성 면역억제에 관한 면역조직화학적 연구 : T 림프구, IL-2 수용기 및 NK세포의 변화를 중심으로 Based on the change of T lymphocytes, Il-2 receptors, and NK cells

김진택,박인식,안상현,최난희,김동환 동국대학교 한의학연구소 1998 東國韓醫學硏究所論文集 Vol.6 No.2

본 실험은 cyclosporin A(CsA)에 의한 시간의 경과에 따른 림프절에서의 세포성 면역억제를 조사하기 위해서 시행된 것으로 BALB/C계 생쥐에 10일동안 CsA(45mg/kg/day) 투여 후 림프절에서의 T 림프구, IL-2 수용기 그리고 자연살해(NK)세포의 분포 변화를 관찰하였다. 대조군의 림프절에서는 L3T4(CD4)에 양성반응을 보이는 도움 T림프구, Ly2(CD8)에 양성반응을 보이는 세포독성 T 림프구 그리고 CD25R에 양성반응을 보이는 IL-2 수용기를 가진 세포는 곁피질(paracortex)과 수질동(medullary sinus)에서 분포하였다. CsA투여 후 처음 3일까지는 이들 양성반웅세포의 분포 변화는 없었으며 양성반응성의 변화도 없었다. 그러나 CsA 투여 7일부터 양성반응 세포수의 감소와 양성반응성의 약화가 관찰되기 시작하였으며 이러한 변화는 시간이 경과하여 14일에 이르렀을 때 가장 큰 감소추세로 나타났다. 한편 NKl.1(CD56)에 양성반응을 보이는 자연살해세포는 피질과 수질에 분포하였으며 CsA 투여 후 시간의 경과에 따라 양성반응 세포수가 감소하였으며, 이러한 감소는 14일에서 가장 큰 것으로 나타났다. 이상의 결과로 미루어보아 CsA 투여는 림프절에서의 IL-2 분비저해를 통해 T 림프구와 NK세포의 활성을 차단하여 선택적이면서 효과적인 세포성 면역억제작용을 하고 있는 것으로 사료된다. Cyclosporin A(CsA) is a selective immunosuppressive agent that has been credited with improved survival of solid organ allografts. Lymph node of BALB/C mouse administered CsA immunohistochemically observed to understand immunosuppressive effects of CsA on T lymphocytes, IL-2 receptors, and natural killer NK cells in lymph node. CsA orally administered daily for 10days at the dose 4smg/kg/day/. The lymph node were obtained at day 3, 7, and 14 after CsA administration and embeded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25), and NK-1.1(CD56). There were little changes of reactive degree and number of helper T lymphocytes, cytotoxic T lymphocytes, IL.-2 receptors, and NK cells at day 3 after CsA administration, but they began to decrease at day 7. These decrease were greatest at day 14. The helper T lymphocytes, cytotoxic T lymphocytes, IL-2 receptors, and NK cells distributed in paracortex and medullary sinus. These results indicated that the secretion of IL-2 began to decrease at day 7 after CsA administration and subsequently to suppress T lymphocytes and NK cell as components of cell-mediated immunity.

白朮散과 白扁豆가 白鼠 小腸과 大腸 杯狀細胞內 粘液質에 미치는 影響

金鎭澤,金兌勳,金東煥 동국대학교 한의학연구소 1993 東國韓醫學硏究所論文集 Vol.2 No.2

This study was performed to identify the effect of Backchulsan and Backpeundu, on the mucosubstances of the goblet cells in the small and large intestines of the mouse. After injected castor oil into oral cavity, the experimental animals were administrated with 5.952mg per 30g body weight of Backchulsan, with 0.519mg per 309 body weight of Bckpeundu. The specimens used in this study were obtained from the small and large intestines of the mouse. All specimens were stained with periodic acid-Schiff reaction, alcian blue pH 2.5 and alcian blue pH 1.0 procedures. The results were as follows 1. The most remarkable decrease of neutral and acid mucosubstance was observed in the control group 1 and 6 hours after castor oil administration. 2. Increase of neutral and mucosubstance was observed in Backchulsan administration group 3 hours in comparision with that of control group administrated of castor oil, especially of the upper and lower villi regions. 3. Increase of neutral and acid rnucosubstance was observed in Backpeundu administration group 6 hours in comparision with that of control group administration of castor oil. 4. The most remarkable increase of neutral and acid mucosubstance was observed in Backchulsan administration group after 3 hours in comparision with that of Backpeundu administration group.

0.2% 불화나트륨 용액으로 5년간 양치한 초등학교 아동의 영구치 우식증 예방효과

손석환,이재화,김지영,김진범 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.2

Supervised weekly fluoride mouthrinsing(FMR) program with 0.2 percent neutral sodium fluroide solution has been implemented at primary schools in Pusan, Korea since 1995 to prevent the prevalent dental caries. Children of sixth grade in 2000 who had joined the weekly FMR program at four primary school in Pusan with a 0.2 percent sodium fluoride solution for five years from first grade in 1995 to fifth grade in 1999 were selected as experimental group. Children of sixth grade in 2000 who had not joined the FMR program until sixth grade and had not drunk fluoridated pulic water at two primary schools in the Bukgu(Norhtern) District of Ulsan were selected as control group. Samples were 295 in FMR group and 312 in control group. To estimate caries preventive effect, epidemiologic dental survey was performed at the schools of FMR and control group in 2000 for 11-year-old children. The surveyed data were analyzed with SPSS statistical package. The obtained results were as follws. 1. Children who experienced caries on permanent teeth were 71.2% in FMR group and 83.3% in control group. 12.1% of DMF rate decreased in FMR group compared to control group. 2. The DMFT scores were 2.68 in FMR group and 3.29 in control group. The difference of DMFT scores was 0.61 between fluoride mouthrinsing group. The caries reduction rate from 5-year FMR program was calculated as 18.5% by the difference of DMFT scores between FMR group and control group. 3. The DMFS scores were 4.04 in FMR group and 5.05 in control group. The difference of DMFS scores was 1.01 between FMR group and control group. The caries reduction rate from 5-year FMR program was calculated as 20.0% by the difference of DMFS scores between FMR group and control group. 4. Significant Caries Index of permanent teeth was 5.46 in FMR group and 6.02 in control group. 5. We suggest that fluoride mouthrinsing program should be developed to all primary schools to prevent dental caries.

고탄소 마르텐사이트-베이나이트 2상 혼합조직강의 베이나이트 변태특성 및 기계적 성질

안진환,김순호,성장현 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.-

This study carried out to investigate into the influence of silicon addition on charpy impact values, transformation kinetics and morphology of lower bainite in high carbon steel with a mixed structure of martensite and lower bainite. The width of lower bainite plate was decreased and the transformation kinetics appeared to be fast with the addition fo silicon. The transformation cirves of lower bainite showed to be a sigmoid shape representing the typical nucleation and growth mechanism. Charpy impact values showed linearly increasing tendency with increasing the volume fraction of bainite and tempering temperature for the mixed structure of martensite and bainite. The chaning range of impact value with the variation of the volume fraction of bainite or appeared to be remarkable, except at the 600℃ tempering, with the addition of silicon. The improvement effect of impact value on tempering was depressed with the addition of silicon. This phenomenon was considered to have an retardation effect on carbide precipitation by silicon.

장기 알콜투여가 생쥐의 간 및 신장, 위장 조직 손상에 미치는 영향

김진택,김동환,안상현 東國大學校韓醫學硏究所 1994 東國韓醫學硏究所論文集 Vol.3 No.-

Alcohol is a major risk factor for several diseases and excessive, long-term alcohol consumption are cause physical alteration-fatty liver, hepatitis, cirrhosis, breaking down, Wernicke-karasakoffs syndrome, weight loss, and poor immunity-in virtually all organ and tissue, This study was observed that liver, kindney, and stomach were altered in mouse by the effect of chronic alcohol administration. The mouse were sacrificed to obtain the tissue after mouse were orally injected with 25% ethanol 18㎖/kg/day for 120days. The tissue were stained by hematoxylin and eosun and then obseved by light microscope. The results of this study were as follows : 1. The congestion was appeared in liver after 120days alcohol administration 2. The destruction of glomerulus were increased and parietal cell of Bowman's capsule were swelled such as cuboidal cell after 12days alcohol administration. The congestion was appeared in alcohol administrated group. 3. The mucosa and gasstric pit were destructed and the ulceration was appeared in stomach after 120days administration. The parietal cells and chief cells were damaged. Above results were shown that the tissue were damaged by chronic alcohol administration.

Intranasal Yaccination with Peptides and Cholera Toxin Subunit B as Adjuvant to Enhance Mucosal and Systemic Immunity to Respiratory Syncytial Virus

Kim, Hyoung-Jin,Kim, Jung-Kwon,Seo, Sang-Byum,Lee, Hoan-Jong,Kim, Hong-Jin 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

Amino acid sequences 200-225 and 255-278 of the F protein of human respiratory syncytial virus (HRSV) are T cell epitopes (Bourgeois of al., 1991 , Corvaisier of al., 1993). Peptides cor-responding to these two regions were synthesized and coupled with keyhole limpet haemocyanin (KLH). The two conjugated proteins were administered intranasally to BALB/C mice alone or together with cholera toxin B (CTB). ELISAs revealed that the mixture of the conjugates with CTB increased not only the systemic response but also the mucosal immune response of the saliva. The systemic response was lower and the mucosal immune response was undetectable in mice immunized with the conjugates on their own. These results suggest that these two peptide sequences are effective epitopes for inducing systemic and mucosal immune responses in conjunction with CTB, and may provide the basis for a nasal peptide vaccine against RSV for human use.

Identification of a Stroma-Mediated Wnt/β-Catenin Signal Promoting Self-Renewal of Hematopoietic Stem Cells in the Stem Cell Niche

Kim, Jin-A,Kang, Young-Ju,Park, Gyeongsin,Kim, Myungshin,Park, Young-Ok,Kim, Hanjun,Leem, Sun-Hee,Chu, In-Sun,Lee, Jun-Seong,Jho, Eek-Hoon,Oh, Il-Hoan Wiley (John WileySons) 2009 Stem Cells Vol.27 No.6

<P>With contrasting observations on the effects of beta-catenin on hematopoietic stem cells (HSCs), the precise role of Wnt/beta-catenin signals on HSC regulation remains unclear. Here, we show a distinct mode of Wnt/beta-catenin signal that can regulate HSCs in a stroma-dependent manner. Stabilization of beta-catenin in the bone marrow stromal cells promoted maintenance and self-renewal of HSCs in a contact-dependent manner, whereas direct stabilization in hematopoietic cells caused loss of HSCs. Interestingly, canonical Wnt receptors and beta-catenin accumulation were predominantly enriched in the stromal rather than the hematopoietic compartment of bone marrows. Moreover, the active form of beta-catenin accumulated selectively in the trabecular endosteum in 'Wnt 3a-stimulated' or 'irradiation-stressed,' but not in 'steady-state' marrows. Notably, notch ligands were induced in Wnt/beta-catenin activated bone marrow stroma and downstream notch signal activation was seen in the HSCs in contact with the activated stroma. Taken together, Wnt/beta-catenin activated stroma and their cross-talk with HSCs may function as a physiologically regulated microenvironmental cue for HSC self-renewal in the stem cell niche.</P>

Microenvironmental Remodeling as a Parameter and Prognostic Factor of Heterogeneous Leukemogenesis in Acute Myelogenous Leukemia

Kim, Jin-A,Shim, Jae-Seung,Lee, Ga-Young,Yim, Hyeon Woo,Kim, Tae-Min,Kim, Myungshin,Leem, Sun-Hee,Lee, Jong-Wook,Min, Chang-Ki,Oh, Il-Hoan American Association for Cancer Research 2015 Cancer research Vol.75 No.11

Identification of Cyclicsulfonamide Derivatives with an Acetamide Group as 11β-Hydroxysteroid Dehydrogenase 1 Inhibitors

Kim, Se Hoan,Kwon, Sung Wook,Chu, So Young,Lee, Jae Hong,Narsaiah, Banda,Kim, Chi Hyun,Kang, Seung Kyu,Kang, Nam Sook,Rhee, Sang Dal,Bae, Myung Ae,Ahn, Sung Hoon,Ha, Duck Chan,Kim, Ki Young,Ahn, Jin H The Pharmaceutical Society of Japan 2011 Chemical & pharmaceutical bulletin Vol.59 No.1

<P>In the continuation of our 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) inhibitor research, cyclic sulfonamide derivatives with an acetamide group at the 2-position were synthesized and evaluated for their abilities to inhibit 11β-HSD1. Among this series, Compound <B>34</B> showed good <I>in vitro</I> activity toward human 11β-HSD1, selectivity against 11β-HSD2, microsomal stability, good pharmacokinetic and safety profiles human ether-a-go-go related gene (hERG and cytochrome P450 (CYP)). Also, a docking study explained the activity difference between human and mouse 11β-HSD1.</P>

Integrated Cell Culture-PCR Detection of Enteroviruses and Reoviruses in Water Sources in Gyeonggi-do

Kim, Kyung-A,Kim, Jong-Chan,Ko, Hoan-Uck,Lee, Jung-Bock,Kim, Young-Sug,Park, Yong-Bae,Lee, Myung-Jin,Kim, Myung-Gill,Kim, Jae-Kwan,Park, Eun-Mi 대한미생물학회 2008 Journal of Bacteriology and Virology Vol.38 No.2

The integrated cell culture-PCR (ICC-PCR) method has been suggested as an improved method for detection of viruses in water environments. We tested 57 source waters including finished water samples in Gyeonggi-do for enteric viral contamination using total culturable virus assay (TCVA) using BGMK cells and ICC-PCR. Nineteen of the 57 source water samples (33.3%) exhibited the cytopathic effect (CPE) on BGMK cells and no finished water did exhibited CPE. Nineteen samples (33.3%) of the 57 were positive for reoviruses. For the enteroviruses, only 3 samples (5.3%) of the 57 samples showed positive results. By using ICC-PCR method, 202 flasks from source water samples were positive for enteroviruses and reoviruses. Three samples from source water were positive for both viruses. However, any flasks tested was not co-infected with two types of viruses. While the enteric viral frequencies in TCVA and ICC-PCR were similar, the viral frequency for reoviruses at first passage in two type of method was higher in ICC-PCR (94.7%) than TCVA (56.9%).