Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF-κB Signaling Pathway

Yingying Shan,Bin Jiang,Jiahui Yu,Jiaye Wang,Xiaoli Wang,He Li,Chunmei Wang,JianGuang Chen,Jinghui Sun 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.9

We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4+ and decrease the number of CD8+ in the peripheral blood, and elevate the ratio of CD4+/CD8+. SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF-κB signaling pathways.

Methanobactin-Mediated One-Step Synthesis of Palladium Nanoparticles

Jiaying Xin,Hong-Chen Fan,Sheng-Fu Ji,Yan Wang,Chungu Xia 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.6

The development of palladium nanoparticles (PdNPs) with a narrow size distribution is an important aspect of nanotechnology. Methanobactin (Mb) is a copper-binding small peptide that appears to function as an agent for copper sequestration and uptake in methanotrophs. Here, Mb was shown to bind and catalytically reduce Pd (II) to Pd (0). The one-step synthesis of monodisperse PdNPs using Mb as both coordination agent and reduction agent is reported. Fluorescence spectra, UV-visible spectra, X-ray photoelectron spectroscopy (XPS) and Fourier transform-infrared spectroscopy (FT-IR) suggested that the Mb molecules catalytically reduce Pd (II) to Pd (0) with the concomitant production of PdNPs. The Mb is then adsorbed onto the surface of the PdNPs to form an Mb–PdNPs coordination compound. This avoids secondary nucleation. The PdNPs are small with high monodispersity and are easily synthesized in Mb solution. The PdNPs were extremely stable and resisted aggregation even after several months.

Improved biological synthesis of gold nanoparticles using methanobactin

Jiaying Xin,Kai Lin,Yan Wang,Chungu Xia 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.3

The present study reported a novel approach for the green synthesis of gold nanoparticles (AuNPs) by using methanobactin (Mb) from the spent medium of Methylosinus trichosporium 3011. This paper explored the synthesis of AuNPs under different Mb concentrations, temperatures, and reaction times. The biosynthesized AuNPs were characterized by using UV–Visible spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Comparing with the previous research using hydroquinone as an extra electron donor to synthesis of AuNPs, this research was the first to show that rising temperature improved the formation rate of AuNPs in a higher concentration of Mb itself. The formation of AuNPs was confirmed by surface plasmon resonance using UV–Visible spectrophotometry. Fluorescence spectroscopy experiments revealed that Mb sample showed three prominent peaks. In addition, the fluorescence exhibited by Mb was rapidly quenched upon addition of HAuCl4. According to the image analysis performed on a TEM micrograph, the obtained AuNPs were predominantly spherical with an average size of 19.3 ± 5.5 nm. XPS analysis demonstrated that Mb molecules reduced Au(III) to Au(0). FTIR spectrum confirmed the involvement of biomass groups in capping and reduction of AuNPs, which increased the stability of the synthesized AuNPs.

Genome wide association study on feed conversion ratio using imputed sequence data in chickens

Jiaying Wang,Xiaolong Yuan,Shaopan Ye,Shuwen Huang,Yingting He,Hao Zhang,Jiaqi Li,Xiquan Zhang,Zhe Zhang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.4

Objective: Feed consumption contributes a large percentage for total production costs in the poultry industry. Detecting genes associated with feeding traits will be of benefit to improve our understanding of the molecular determinants for feed efficiency. The objective of this study was to identify candidate genes associated with feed conversion ratio (FCR) via genome-wide association study (GWAS) using sequence data imputed from single nucleotide polymorphism (SNP) panel in a Chinese indigenous chicken population. Methods: A total of 435 Chinese indigenous chickens were phenotyped for FCR and were genotyped using a 600K SNP genotyping array. Twenty-four birds were selected for sequencing, and the 600K SNP panel data were imputed to whole sequence data with the 24 birds as the reference. The GWAS were performed with GEMMA software. Results: After quality control, 8,626,020 SNPs were used for sequence based GWAS, in which ten significant genomic regions were detected to be associated with FCR. Ten candidate genes, ubiquitin specific peptidase 44, leukotriene A4 hydrolase, ETS transcription factor, R-spondin 2, inhibitor of apoptosis protein 3, sosondowah ankyrin repeat domain family member D, calmodulin regulated spectrin associated protein family member 2, zinc finger and BTB domain containing 41, potassium sodium-activated channel subfamily T member 2, and member of RAS oncogene family were annotated. Several of them were within or near the reported FCR quantitative trait loci, and others were newly reported. Conclusion: Results from this study provide valuable prior information on chicken genomic breeding programs, and potentially improve our understanding of the molecular mechanism for feeding traits.

Improved biological synthesis of gold nanoparticles using methanobactin

Xin, Jiaying,Lin, Kai,Wang, Yan,Xia, Chungu 한국응용생명화학회 2015 Applied Biological Chemistry (Appl Biol Chem) Vol.58 No.3

The present study reported a novel approach for the green synthesis of gold nanoparticles (AuNPs) by using methanobactin (Mb) from the spent medium of Methylosinus trichosporium 3011. This paper explored the synthesis of AuNPs under different Mb concentrations, temperatures, and reaction times. The biosynthesized AuNPs were characterized by using UV-Visible spectroscopy, fluorescence spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Comparing with the previous research using hydroquinone as an extra electron donor to synthesis of AuNPs, this research was the first to show that rising temperature improved the formation rate of AuNPs in a higher concentration of Mb itself. The formation of AuNPs was confirmed by surface plasmon resonance using UV-Visible spectrophotometry. Fluorescence spectroscopy experiments revealed that Mb sample showed three prominent peaks. In addition, the fluorescence exhibited by Mb was rapidly quenched upon addition of $HAuCl_4$. According to the image analysis performed on a TEM micrograph, the obtained AuNPs were predominantly spherical with an average size of $19.3{\pm}5.5nm$. XPS analysis demonstrated that Mb molecules reduced Au(III) to Au(0). FTIR spectrum confirmed the involvement of biomass groups in capping and reduction of AuNPs, which increased the stability of the synthesized AuNPs.

Understanding the Increasing Hot Extremes over the Northern Extratropics Using Community Atmosphere Model

Siyu Zhao,Jiaying Zhang,Yi Deng,Na Wang 한국기상학회 2022 Asia-Pacific Journal of Atmospheric Sciences Vol.58 No.3

The past four decades have seen an increase of terrestrial hot extremes during summer in the northern extratropics, accompanied by the Northern Hemisphere (NH) sea surface temperature (SST) warming (mainly over 10°–70°N, 0°–360°) and CO2 concentration rising. This study aims to understand possible causes for the increasing hot extremes, which are defined on a daily basis. We conduct a series of numerical experiments using the Community Atmosphere Model version 5 model for two periods, 1979–1995 and 2002–2018. The experiment by changing the CO2 concentration only with the climatological SST shows less increase of hot extremes days than that observed, whereas that by changing the NH SST (over 10°–70°N, 0°–360°) with constant CO2 concentration strengthens the hot extremes change over mid-latitudes. The experiment with both SST and CO2 concentration changes shows hot extremes change closer to the observation compared to the single-change experiments, as well as more similar simulations of atmospheric circulations and feedbacks from cloud and radiative processes. Also discussed are roles of natural variability (e.g., Pacific Decadal Oscillation and Atlantic Multidecadal Oscillation) and other factors (e.g., Arctic sea ice and tropical SST).

A zonal hybrid approach coupling FNPT with OpenFOAM for modelling wave-structure interactions with action of current

Li, Qian,Wang, Jinghua,Yan, Shiqiang,Gong, Jiaye,Ma, Qingwei Techno-Press 2018 Ocean systems engineering Vol.8 No.4

This paper presents a hybrid numerical approach, which combines a two-phase Navier-Stokes model (NS) and the fully nonlinear potential theory (FNPT), for modelling wave-structure interaction. The former governs the computational domain near the structure, where the viscous and turbulent effects are significant, and is solved by OpenFOAM/InterDyMFoam which utilising the finite volume method (FVM) with a Volume of Fluid (VOF) for the phase identification. The latter covers the rest of the domain, where the fluid may be considered as incompressible, inviscid and irrotational, and solved by using the Quasi Arbitrary Lagrangian-Eulerian finite element method (QALE-FEM). These two models are weakly coupled using a zonal (spatially hierarchical) approach. Considering the inconsistence of the solutions at the boundaries between two different sub-domains governed by two fundamentally different models, a relaxation (transitional) zone is introduced, where the velocity, pressure and surface elevations are taken as the weighted summation of the solutions by two models. In order to tackle the challenges associated and maximise the computational efficiency, further developments of the QALE-FEM have been made. These include the derivation of an arbitrary Lagrangian-Eulerian FNPT and application of a robust gradient calculation scheme for estimating the velocity. The present hybrid model is applied to the numerical simulation of a fixed horizontal cylinder subjected to a unidirectional wave with or without following current. The convergence property, the optimisation of the relaxation zone, the accuracy and the computational efficiency are discussed. Although the idea of the weakly coupling using the zonal approach is not new, the present hybrid model is the first one to couple the QALE-FEM with OpenFOAM solver and/or to be applied to numerical simulate the wave-structure interaction with presence of current.

Scientific Collaboration Networks in China’s System Engineering Subject

Sen Wu,Jiaye Wang,Xiaodong Feng,Dan Lu 보안공학연구지원센터(IJUNESST) 2013 International Journal of u- and e- Service, Scienc Vol.6 No.6

ADOPTING DISSONANCE-SOLVING APPROACH TO INVESTIGATE LUXURY SOCIAL MEDIA ADVERTISING: ROLE OF GAPS IN SELF, PROJECTIVE SELF, INFLUENCER AND BRAND IMAGE

Jie Meng,Jiaying Wang 글로벌지식마케팅경영학회 2019 Global Fashion Management Conference Vol.2019 No.07

A Novel Image Fusion Algorithm Combining with Classification in NCST Domain

Jitao Zhang,Aili Wang,Jiaying Zhao 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.10

Image fusion is an important branch of information fusion, which is widely used in various fields. At present, the image fusion method is mainly aimed at the different frequency information of the images, the images are fused in transform domain. But in practical application, image fusion is used to improve the credibility of the target information and the demand of background information of is not high. Therefore, this paper puts forward an image fusion method combining with image classification. Firstly, the NSCT transform is used to transform the source images, and the K-Means method is used to realize the classification of the target and the background, and the different fusion criteria are used to get the target and the background. The experimental results show that the image fusion based classification method has a better effect on the subjective visual effect and objective evaluation index.