Maintaining Drosha expression with Cdk5 inhibitors as a potential therapeutic strategy for early intervention after TBI

Huang Lu,Xia Li,Nie Tiejian,Cui Bozhou,Lu Jianjun,Lu Fangfang,Fan Feiyan,Ren Dongni,Lu Yuan,Gao Guodong,Yang Qian 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Traumatic brain injury (TBI) is a major cause of death and disability in adults. The pathological process of TBI involves a multifactorial cascade in which kinases have been proven contribute to interactions between relevant factors and amplification of signaling cascades. Cyclin-dependent kinase 5 (Cdk5) is a promising kinase that has been implicated in various brain disorders, including TBI. However, the mechanism by which Cdk5 induces neuronal damage remains unclear. Here, we show for the first time that Drosha, a key enzyme in microRNA biogenesis, is a pivotal substrate of abnormally activated Cdk5. Cdk5-mediated phosphorylation decreases Drosha expression and exacerbates nerve injury in TBI. We proved that maintaining Drosha expression via the administration of repurposed Cdk5 inhibitors that were previously studied in clinical trials is a promising approach for the early treatment of TBI. Together, our work identifies Drosha as a novel target for neuroprotective strategies after TBI and suggests Cdk5-mediated regulation of Drosha expression as a potential therapeutic strategy for early TBI intervention.

Trajectory Learning and Reproduction for Tracked Robot Based on Bagging-GMM/HSMM

Lu En,Zhao Zhan,Yin Jianjun,Luo Chengming,Tian Zhongming 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.6

Learning and reproducing the tracked robot’s demonstration trajectory is a promising intelligent path planning solution that can summarize and extract the relevant characteristics of the tracked robot’s desired trajectory. However, the existing methods are difficult to ensure the robustness when there are deviations in the demonstration trajectory data or when the task constraints are added. In order to address the problem, this paper proposes a novel trajectory learning and reproduction method for tracked robots that is based on Bagging algorithm and GMM/HSMM. The Bagging algorithm is used to randomly resample the demonstration trajectory data and construct the sub trajectory data sets. Then, GMM/HSMM is used to train and learn these sub trajectory data sets, and the output probability density function of the hidden state and the Gaussian component with the largest average position probability are selected as the motion elements. According to the mean and variance of these motion elements, the least square method is used to reconstruct the tracked robot’s trajectory. On the basis of using the IPSO algorithm to optimize the position of constraint points in each sub trajectory data sets, combined with the weight method, the learning results are integrated to realize the trajectory reproduction of a tracked robot under task constraints. The final results and analysis show that the proposed method can successfully realize the trajectory learning and reproduction of tracked robot, as well as ensure that the reproduced trajectory can pass through the required task constraint points without increasing the algorithm’s complexity.

The technical analysis and study of decommissioning and abandonment of offshore oil field surface facilities and subsea system

Lu, Guihua,Li, Hongsheng,Xiao, Jianjun,Li, Zhihao,Xu, Minhang Techno-Press 2016 Ocean systems engineering Vol.6 No.3

With more than 100 years exploration and development of offshore oil, more and more offshore oil fields will gradually lose the economic profit to operate. In this article, to take the target oil field for example, the procedure of decommissioning for FPSO, TCMS, subsea system and well abandonment have been analyzed. Meanwhile, the environment impact and mitigation measure have been proposed. The successful project experience will provide a guide line for the offshore facilities decommissioning and abandonment.

Vertical Migration of Sound Scatterers in the Southern Yellow Sea in Summer

Lu, Lian-Gang,Liu, Jianjun,Yu, Fei,Wu, Wei,Yang, Xiaodong The Korean Society of Oceanography 2007 Ocean science journal Vol.42 No.1

Acoustic volume backscattering strength data were collected and Conductivity Temperature Depth (CTD) measurements were conducted in the southern Yellow Sea in summer 2005 and 2006. The high temporal and vertical resolution acoustic data measured with a 307 kHz Acoustic Doppler Current Profiler (ADCP) and a 250 kHz acoustic Doppler profile (ADP) had dominant diel variation, which resulted from vertical migration of sound scatterers. Some scatterers congregating in the bottom layer in the daytime migrated upward at dusk, and migrated downward into the bottom layer at dawn. The migration speeds were estimated. More than 33 days data show that the diel migration varies with time. The feature of migration measured with ADCP and ADP is consistent to some extent with what is described in the study on vertical migration of zooplankton in the southern Yellow Sea with conventional net samples.

Taxonomy of fungal complex causing red-skin root of Panax ginseng in China

Lu, Xiao H.,Zhang, Xi M.,Jiao, Xiao L.,Hao, Jianjun J.,Zhang, Xue S.,Luo, Yi,Gao, Wei W. The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.3

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits the production of ginseng in China. The disease has long been thought to be a noninfectious physiological disease, except one report that proved it was an infectious disease. However, the causal agents have not been successfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in Northeast China. Fungi were isolated from the lesion and identified based on morphological characters along with multilocus sequence analyses on internal transcription spacer, β-tubulin (tub2), histone H3 (his3), and translation elongation factor 1α (tef-1α). Pathogens were confirmed by inoculating the isolates in ginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classified into 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani, F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel species I. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had the highest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated were pathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

Occurrence of three intracellular symbionts (Wolbachia, Arsenophonus, Cardinium) among ants in southern China

Lei Wang,Jianjun Jiang,Yijuan Xu,Ling Zeng,Yongyue Lu 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4

Many ants are commonly infected bymaternally inherited endosymbionts.Weexamined the prevalence of three bacterial endosymbionts (Wolbachia, Arsenophonus, and Cardinium) in ant populations in southern China. The results showed that the proportion of ant samples infected byWolbachia and Arsenophonuswas 30.20% and 18.79%, respectively. Cardiniumwas not found in any of our samples. Our results also indicated that more than 50% of the ant species (30 of 56) tested were infected by maternally inherited bacteria. Of the 56 ant species, 35.71% were infected byWolbachia and 37.50%were positive for Arsenophonus. Co-occurrence of Arsenophonus and Wolbachia was found in the same colony in 7 of 56 ant species. This study suggests that Wolbachia and Arsenophonus are the main inherited bacteria in ants in southern China. These endosymbiontsmay have strong impacts on ant biology and can be manipulated for pest management.

Taxonomy of fungal complex causing red-skin root of Panax ginseng in China

Xiao H. Lu,Xi M. Zhang,Xiao L. Jiao,Jianjun J. Hao,Xue S. Zhang,Yi Luo,Wei W. Gao 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits theproduction of ginseng in China. The disease has long been thought to be a noninfectious physiologicaldisease, except one report that proved itwas an infectious disease. However, the causal agents have not beensuccessfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in NortheastChina. Fungi were isolated from the lesion and identified based on morphological characters alongwith multilocus sequence analyses on internal transcription spacer, b-tubulin (tub2), histone H3 (his3),and translation elongation factor 1a (tef-1a). Pathogens were confirmed by inoculating the isolates inginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classifiedinto 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani,F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel speciesI. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had thehighest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated werepathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

Preparation, Characterization and Luminescence Properties of a Novel 1,10-Phenanthroline-Functionalized Polyimide and Its Europium(III) Complexes

Zhiyong Chen,Jianjun Lu,Xuping Li,Xuting Jin,Lijuan Shi,Miaoqing Liu,Zhilin Sun 한국고분자학회 2015 Macromolecular Research Vol.23 No.9

A novel 1,10-phenanthroline-functionalized polyimide (CMPI-Phen) was prepared as polymer matrix by the nucleophilic substitution reaction between chloromethylated polyimide (CMPI) and 5-amino-1,10-phenanthroline (NH2-Phen). Then, two kinds of luminescent materials of 1,10-phenanthroline-functionalized polyimide containing Eu(III) complexes, were obtained by two different methods. Their structures and properties were characterized by Fourier transform infrared (FTIR) spectroscopy, 1H nuclear magnetic resonance (NMR) spectroscopy, elemental analysis, inductively coupled plasma (ICP), gel permeation chromatography (GPC), thermogravimetric analysis (TGA), X-ray diffraction (XRD), luminescence spectrometry, and luminescence decay analysis. Investigations revealed that both the CMPI-Phen-Eu(III) and CMPI-Phen-Eu(TTA)3 display highly efficient red emissions, suggesting their potential application as luminescent materials. However, compared with CMPI-Phen-Eu(III), CMPI-Phen-Eu(TTA)3, where TTA stands for thenoyltrifluoroacetone, exhibits more excellent and stable fluorescence intensity and longer luminescence lifetime (134.30 μs). The weight-average molecular weights of CMPI-Phen-Eu(III) and CMPI-Phen- Eu(TTA)3 are 2.40×104 and 3.11×104, respectively. The Eu contents of CMPI-Phen-Eu(TTA)3 and CMPI-Phen-Eu(III) were measured by ICP to be 7.00% and 5.92%, respectively. TGA demonstrated that both CMPI-Phen-Eu(III) and CMPI-Phen-Eu(TTA)3 have high thermal stability and their 5% loss weight temperatures were 356 and 280 oC, respectively. Moreover, both CMPI-Phen-Eu(III) and CMPI-Phen-Eu(TTA)3 were soluble in polar solvents such as dimethylformamide (DMF), dimethylacetamide (DMAc), 1-methyl-2-pyrrolidone (NMP), and dimethylsulfoxide (DMSO) under heating conditions, and could be easily cast into tough thin films.

Amino ionic liquids-modified magnetic core/shell nanocomposite as an efficient adsorbent for dye removal

Jingjing Cheng,Lijuan Shi,Jianjun Lu 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.36 No.-

Amino ionic liquids modified superparamagnetic mesoporous core/shell nanocomposite (Fe3O4@n-SiO2@mSiO2@DHIM-NH2) was synthesized to remove orange II and amaranth from water. Due to themultiple active sites of the introduced ionic liquids, the adsorbent presents superior adsorptionperformance and instantaneous adsorption capacity. The maximum adsorption capacities are reached atpH 2 due to the protonation of amino groups, which are 153.06 and 84.40 mg g 1 for orange II andamaranth, respectively. The good stability, rapid adsorption kinetics, large adsorption capacity, and highseparation efficiency make Fe3O4@nSiO2@mSiO2@DHIM-NH2 an efficient adsorbent for removinganionic contaminant from water.

Numerical analysis of the formation mechanism and suppression method of the reverse flow in a semi-open centrifugal pump

Like Wang,Jinling Lu,Weili Liao,Wei Wang,Jianjun Feng,Yaping Zhao 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.9

Reverse flow has a detrimental effect on the stable and safe operation of centrifugal pumps. To study the formation mechanism and suppression of the reverse flow, a semi-open centrifugal pump with circumferential groove in the shroud was simulated. Then, the flow field and pressure fluctuation were analysed. The absolute flow angle at the blade inlet nearing the shroud was close to 180° because of the joint action of the leakage flow and blade inlet impact under low flow rate. This phenomenon resulted in the formation of a low-speed region and the reverse flow and low-frequency pressure fluctuation. The circumferential groove provided a channel for the leakage flow, which could quickly pass through the groove, and reduced the absolute flow angle at the blade inlet nearing the shroud and weakened the trend of the tip leakage flow to upstream. The low-frequency pressure pulsation was eliminated, and the amplitude of the blade passing frequency was reduced under 0.7 Q d (Q d is the design flow rate). The reverse flow thickness coefficient became zero with the circumferential groove. The proportion of the reverse flow volume to the volume of inlet pipe decreased from 14.7 % to 2.2 % under 0.4 Q d . This research indicated that the circumferential groove arranged in the shroud could effectively suppress or eliminate reverse flow.