고강도 콘크리트를 사용한 플랫 플레이트 구조의 기둥-슬래브 접합부 강성에 관한 연구

김형기,유제준 대한건축학회 2005 大韓建築學會論文集 : 構造系 Vol.21 No.2

In this study, the four column-slab connections in flat plate system using high strength concrete exceeding 60㎫, modeled after a typical reinforced concrete flat plate building, were tested under combined gravity and lateral loadings. The variables selected for this study were the slab reinforcement ratio and the level of slab gravity load. The test results were compared with the stiffness predictions of elastic finite element analysis, the ACI equivalent frame method and the AIJ method. The followings were found: 1) The stiffness of column-slab connection under gravity loading reduced as the slab gravity load increased. At the level of service gravity load, the gravity stiffness was about 70 percent of initial stiffness. 2) The lateral stiffness of column-slab connection decreased with increasing the lateral deflection and was dependent on the level of slab gravity "load and slab reinforcement percentage. 3) The initial lateral stiffness was about 50 percent of elastic finite element analysis in lateral drift ratio of 0.0625 percent. This was attributed to the accelerated slab cracking around the connection as a result of the increased gravity load moments. 4) The lateral stiffness varied from 24 to 37 percent of elastic finite element analysis and from 17 to 26 percent of ACI equivalent frame method in lateral drift ratio of 0.2 percent. This result gave agreement with other researcher's test result of column-slab connection in flat plate system using normal strength concrete.

호텔산업에서 공급사슬관리(SCM)를 통한 물류생산성 증진에 관한 실증연구

한한수,김흥유,임재욱 한국생산성학회 2002 生産性論集 Vol.16 No.3

Recently, Hotels re faced with competitions form other highly upgraded calering business and the increased lobor costs. As one of the methods to solve these difficalties. Stratgic supply could be considered for a hotel business. Actually, SCM(Supply Chain Management) is a theory of a general administion. In recent, a lots of companies over the world have implemented SCM in their supply management and have been succed. Thus, this study is to consider about the possibility if SCM could be used in the hotel business. The survey is enhanced with the staffs who is charge of the purchasing of 60 hotels and analyzed by SPSS 10.0 and AMOS 4.0. This study is only concerned to material supply. But, in the hotel work, it could be also important for service supplies such as order information processing.

심장판막에서 TGF-β1의 생성과 분포

고재기,김남균,김민호,채제건,고규영 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.7

합성펩타이드를 이용한 영양배엽세포-특이 가토 다클론 항혈청의 제작

이희섭,오재민,김정중,문형배,김원신,이황희 원광대학교 생명공학연구소 1995 생명공학연구소보 Vol.3 No.1

Within the last few years, a different approach to generating protein-reactive antibodies has been developed that has several advantages over conventional immunization. This involves synthesizing short peptide sequences, coupling them to immunogenic carrier molecules, and immunizing animals with the conjugates. 3βHSD(3β-hydroxy-5-ene steroid dehydrogenase; EC 1.1.1.145) is the enzyme of the plasma membrane of human trophoblast and it's cDNA sequence was identified by Nickon et al(Molecular cloning and expression of human trophoblast antigen FDO161G and its identification as 3β-hydroxy-5-ene steroid dehydrogenase. J Reprod Fert 1991;149;156). For the production of trophoblast-specific antibody, we synthesized three oligopeptides that are epitope sites chosen from cDNA sequence of 3βHSD. Oligopetides were coupled with KLH(keyhole limpet hemocyanin) under 25% glutaraldehyde. The trophoblast-specific rabbit polyclonal antisera was produced by conventional methods. This antisera reacts with a 43kDa protein in human placental lysate by Western blotting analysis and The syncytiotrophoblasts and cytotrophoblasts from villi are stained positively with this antisera by immunohistochemistry. Villous trophoblasts were cultured in methionine-free media for 1 hour and [^(35)S]-Methionine for 24 hours. Media and cell lysate were immunoprecipitated with this antisera and 12% SDS-PAGE was performed. In fluorography, bend was not noted in media and 43kDa band was noted in medis and 43kDa band was noted in lysate. It was concluded that anti-3βHSD antibody produced by synthetic peptide was specific to trophoblasts and 3βHSD was membrane-bound protein of trophoblasts.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

Slurry 반응조를 이용한 퇴적준설물내 질소의 생물학적처리에 관한 연구

정연규,배범한,김용학,전제철,엄성범 연세대학교 산업기술연구소 2002 논문집 Vol.33 No.2

The eutrophication control and prevention can be accomplished through removal of the nutrients known as main contributors. However, for the case of lakes and rivers where significant amounts of the nutrients were already entered, there should be a limit on the eutrophication control. Accordingly, it is indispensable to remove the nutrients present in sediments in view of both obtaining water resources and preserving the environment, and, therefore, this study focuses on nitrogen removal among the nutrients in sediments. For nitrogen removal in sediments, nitrification and denitrification were applied as a biological removal process to a slurry reactor available for bioremediation of contaminated soils. With approx. 508㎎/㎏ of T-N concentration in the treated sediments, about 50 % of initial nitrogen concentration was accordingly removed after total 9-day operation of a 10 %-slurry reactor under oxic and anoxic conditions for 4 hrs and 2 hrs, respectively. This means that 25 % of T-N leachates in physical fractions were removed from the sediments through the nitrification and denitrification, and additional 25 % were removed under the same process after extracted from the sediments by a biological mechanism such as organic biodegradation. As a result, 20 % larger nitrogen amounts added were removed after leached from the sediments, which was probably caused by the fact that biological activity was increased by optimization of operating conditions as well as that mineralization and ammonification of organic matters in sediments were elevated by the enlarged population of microorganism after injection of organic C-sources and trace elements. The denitrification was more activated after the injection of organic C-sources and trace elements. The leaching amount of ammonium also increased steeply. The nitrification rate of the slurry reactor was 189 ng N/min·g in dry weight.

충북지역 유기농 옥수수 재배지 유기자재 사용실태 및 토양 화학성 현황

김현주(Hyun-Ju Kim),김충우(Chung-Woo Kim),유주희(Ju-Hee Yu),김미량(Mi-Rang Kim),김민자(Min-Ja Kim),김익제(Ik-Jei KIm),김주형(Ju-Hyoung Kim),이규회(Kyu-Hoi Lee),홍성택(Sung-Taek Hong) 한국토양비료학회 2018 한국토양비료학회 학술발표회 초록집 Vol.2018 No.10

Effects of Soybean Sauce and Pre-rigor Muscle on Physicochemical Properties of Frozen Hanwoo Patties

Kim, Hyun-Wook,Choi, Ji-Hun,Choi, Yun-Sang,Han, Doo-Jeong,Kim, Hack-Youn,Lee, Mi-Ai,Kim, Si-Young,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2011 한국축산식품학회지 Vol.31 No.1

In this study, we evaluated the physicochemical and sensorial properties of frozen Hanwoo beef patties prepared from prerigor and post-rigor muscle with soybean sauce or sodium chloride. The pH of the soybean sauce treatments was lower than the sodium chloride treatments (p<0.05); however, the Hanwoo patty prepared from pre-rigor muscle with soybean sauce had a lower cooking loss and smaller diameter than the Hanwoo patty prepared under the same conditions from post-rigor muscle (p<0.05). The addition of the soybean sauce inhibited lipid oxidation due to the antioxidative materials in soybean sauce such as melanoidin. The hardness of patties prepared from pre-rigor treatment was lower relative to those prepared from post-rigor muscle (p<0.05); however, there was no significant difference between springiness, cohesiveness, and chewiness (p>0.05). In the sensory evaluation, patties prepared from pre-rigor muscle with soybean sauce had a significantly high score in all traits (p<0.05); thus, pre-rigor muscle with soybean had the highest overall acceptability. Furthermore, the addition of soybean sauce to pre-rigor muscle produced similar effects as those observed for the addition of sodium chloride in regards to psychochemical properties. Moreover, meat products produced from pre-rigor muscle will have better antioxidative and sensorial properties.

Effects of Sea Tangle (Lamina japonica) Powder on Quality Characteristics of Breakfast Sausages

Kim, Hyun-Wook,Choi, Ji-Hun,Choi, Yun-Sang,Han, Doo-Jeong,Kim, Hack-Youn,Lee, Mi-Ai,Kim, Si-Young,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2010 한국축산식품학회지 Vol.30 No.1

Breakfast sausages containing 1, 2, 3, and 4% sea tangle powder (Lamina japonica) were prepared. No differences were found in moisture, protein, and fat contents among the control and treatments. However, the ash content increased with increasing amounts of sea tangle powder (p<0.05). The pH levels in the treated samples were lower than the control in both the meat batters and the breakfast sausages (p<0.05). The $L^*$ and $a^*$ values of the meat batters and breakfast sausages were decreased by the addition of the sea tangle powder, and the control had the highest $b^*$ value (p<0.05). The added sea tangle powder improved cooking loss and improved emulsion stability. The T4 sample (containing 4% sea tangle powder) was shown to have the lowest cooking loss and water loss (p<0.05). The hardness, gumminess, and chewiness of the treatments increased compared to the control due to the presence of dietary fibers in the sea tangle. In the sensory evaluations, the 1% sea tangle powder treatment received a lower color score, but received significantly higher scores for flavor, tenderness, and juiciness (p<0.05). Collectively, the breakfast sausage containing 1% sea tangle powder was determined to have the highest overall acceptability. Altogether, the best results, in terms of physicochemical and sensory properties, were obtained for the breakfast sausage containing 1% sea tangle powder.

Ovarian Features after 2 Weeks, 3 Weeks and 4 Weeks Transdermal Testosterone Gel Treatment and Their Associated Effect on IVF Outcomes in Poor Responders

Kim, Chung-Hoon,Ahn, Jun-Woo,Moon, Jei-Won,Kim, Sung-Hoon,Chae, Hee-Dong,Kang, Byung-Moon 한국발생생물학회 2014 발생과 생식 Vol.18 No.3

This study was performed to investigate the effect of of transdermal testosterone gel (TTG) on controlled ovarian stimulation (COS) and IVF outcomes and ovarian morphology according to pretreatment duration in poor responders. A total of 120 women were recruited for this pilot study. They were randomized into control, 2 weeks, 3 weeks or 4 weeks TTG treatment groups. For three TTG treatment groups, 12.5 mg TTG was applied daily for 2 weeks, 3 weeks or 4 weeks in preceding period of study stimulation cycle. After 3 weeks of TTG pretreatment, significant increase of antral follicle count (AFC) and significant decreases of mean follicular diameter (MFD) and resistance index (RI) value of ovarian stromal artery were observed (p=0.026, p<0.001, p<0.01, respectively). The total dose of rhFSH administered for COS significantly decreased after 3 and 4 weeks TTG treatment both compared with control group (p<0.001, p<0.001). The numbers of oocytes retrieved and mature oocytes were significanty higher in 3 and 4 weeks TTG treatment groups than control group (p<0.001, p<0.001 in the number of oocytes retrieved; p<0.001, p<0.001 in the number of mature oocytes). The clinical pregnancy rate and live birth rate were increased only in 4 weeks TTG treatment group compared with control group (p=0.030 and p=0.042, respectively). These data demonstrated that TTG pretreatment for 3 to 4 weeks increases AFC and ovarian stromal blood flow, thereby potentially improving the ovarian response to COS and IVF outcome in poor responders undergoing IVF/ICSI.