Temporal cDNA microarray analysis of gene expression in human hepatocellular carcinoma upon radiation exposure.

Jeong, Jaemin,Hong, Su-Jin,Ju, Yeun-Jin,Kim, Bu-Yeo,Park, Myung-Jin,Kim, Tae-Hwan,Park, Chul-In,Choi, Kang-Yell,Cho, Myung-Haeng,Kim, Sang-Hoon,Lee, Hansoo,Lee, Kee-Ho National Hellenic Research Foundation 2006 ONCOLOGY REPORTS Vol.15 No.1

<P>Radiotherapy is not commonly used for the treatment of human hepatocellular carcinoma, due to its poor response rate and poor tolerance of normal liver to ionizing radiation. Recently developed microarray technology makes it possible to verify genes responsive to anticancer therapy of human cancers by simultaneous analysis of gene expression profiles. In the present study, the expression profile of radiation-responsive genes in human hepatocellular carcinoma was evaluated through time-dependent cDNA microarray analysis of expressional variation, following exposure to ionizing radiation. Upon exposure to radiation, more than 13% of genes in both radiation-resistant and -sensitive cells responded to radiation. Time-dependent analysis of radiation-responsive genes revealed that, irrespective of radiation sensitivity, greatly different subsets of genes sequentially participated in cellular response to radiation at their specific activation or deactivation time points. The majority of radiation-responsive genes were differentially but not commonly expressed between radiation-resistant and -sensitive cells. When these differentially regulated genes were classified according to their physiological and functional characteristics and radiation sensitivity, it was prominently obvious that DNA repair-promoting genes were up-regulated in radio-resistant cells and down-regulated or unchanged in radiation-sensitive cells. The present findings indicate that different subsets of genes are sequentially working and DNA repair capacity may control the radiation sensitivity of human hepatocellular carcinoma cells more than any other physiological factor.</P>

뉴스 감성 분석을 이용한 돼지고기 가격변화 예측 모델

정재민(JaeMin Jeong),서한빛(Hanbit Seo),고은영(Eun-young Go),이의종(Eui-Jong Lee) 한국정보과학회 2023 한국정보과학회 학술발표논문집 Vol.2023 No.6

신박물관으로서 ‘버나비 빌리지 뮤지엄’에 대한 연구

정재민(Jeong, Jaemin),배은석(Bae, Eun Sok) 한국박물관학회 2021 博物館學報 Vol.- No.40

캐나다 브리티시 컬럼비아(British Columbia)주 버나비(Burnaby)시에 위치한 버나비 빌리지 뮤지엄(Burnaby Village Museum)은 1920년대 마을을 그대로 복원하여 재현하고 있으며, 각 시설에 시대상을 해석해주는 해석가가 존재한다. 이는 과거 특정 시대를 재현하여 방문객에게 경험적 지식을 제공하는 ‘리빙뮤지엄’의 형태와 동일하다. 또한, 박물관 내 탑승 가능한 체험형 전시물은 지역 주민의 손에 의해 복원되었고, 지속해서 지역 대학 및 지역 정부와 협업해 지역 전체를 터전으로 전시를 진행하고 있기도 하다. 이는 자연, 생태, 산업, 문화 등 모든 것을 유산으로 포함하고 지역 주민이 자발적으로 참여해 지역 전체가 유기적으로 구성되는 ‘에코뮤지엄’의 속성을 드러낸다. 특히 빈 부지에 소멸 위기의 유산을 복원하는 면에서 ‘테마파크형 에코뮤지엄’의 속성이 두드러졌다. 이렇듯 버나비 빌리지 뮤지엄은 ‘리빙뮤지엄’과 ‘에코뮤지엄’의 특성과 속성을 모두 가지며 양가적이고 복합적인 특징을 보인다. 따라서 두 박물관의 접점에서 버나비 빌리지 뮤지엄의 유형을 찾을 수 있으며, 그것은 곧 박물관의 정체성으로 이어진다. 다양한 지역 이벤트를 개최하며 지역 공동체의 터전이 되고 있는 버나비 빌리지 뮤지엄은 지속적인 ‘기억의 수집과 보존’을 통하여 지역 주민은 물론이고 방문객과 소통하기 위해 꾸준히 노력하고 있다. 이는 ‘새로운 박물관이 사람들에게 휴식과 영감을 줄 수 있는 장소가 되어야한다’는 논의를 끌어내며, 과거 전통적 박물관에 대한 대안적 장소, 즉, 일종의 헤테로토피아로서 신박물관이 가지는 역할과 의의를 다시 한 번 생각해보게 한다. The Burnaby Village Museum, located in Burnaby, British Columbia, Canada, recreates the setting of the region in the 1920s, and each facility in the museum has an interpreter who makes history come alive, reproducing a specific historical character in the past. This is identical to the form of ‘Living museum’ that simulates a specific era and provides visitors with experiential knowledge on past history. Besides, the interactive exhibits which visitors can ride on were restored by the hands of the local residents in Burnaby, and are continuously displaying various exhibitions in cooperation with the local university and the government through the whole local area. This shows the attribute as Ecomuseum in that the realm of the ecomuseum embraces every inheritance including nature, ecology, industry, and culture, and the entire area is organically composed by its local volunteers. In particular, the attribute as Theme Park-Style Ecomuseum, which restores legacies in danger of extinction on an empty site, was distinctive. Likewise, Burnaby Village Museum has the ambivalent and complex characteristics of both ‘Living museum’ and ‘Ecomuseum.’ Thus, it can be concluded that the Burnaby Village Museum can be found at the junction of the two museum types, which gives birth to a unique identity. The Burnaby Village Museum, which keeps hosting various local events for the local community, makes an effort to communicate with visitors as well as local residents through ‘continuous collection and preservation of memories.’ This leads to the discussion that “new museums should be a specific place where people can rest and get inspired”, appealing the role and significance of a new museum as an alternative to the old-fashioned traditional museum, that is, a kind of heterotopia.

딥-뉴럴 네트워크의 파라미터 최적화

김민정(Min-Jeong Kim),임병준(Byungjoon Lim),임승현(Seunghyun Lim),정재민(Jaemin Jung),이종학(Jong-Hak Lee),이정근(Jeong-Gun Lee) 대한전자공학회 2018 대한전자공학회 학술대회 Vol.2018 No.11

Establishment of an optimal occupant behavior considering the energy consumption and indoor environmental quality by region

Kim, Jimin,Hong, Taehoon,Jeong, Jaemin,Lee, Myeonghwi,Lee, Minhyun,Jeong, Kwangbok,Koo, Choongwan,Jeong, Jaewook Elsevier 2017 APPLIED ENERGY Vol.204 No.-

<P>Reducing a building's energy consumption and providing better indoor environmental quality (IEQ) are the two major issues that building professionals are facing all over the world. It is not easy, however, to simultaneously address both issues. Therefore, this study aimed to establish the optimal occupant behavior that can simultaneously reduce total energy consumption and improve the IEQ, using an energy simulation and optimization tool. This study also developed an integrated IEQ score by combining three different IEQ indices (i.e., thermal comfort, indoor air quality (IAQ), and visual comfort) for building users to easily understand the IEQ condition. To analyze the effects of occupant behavior by region, the education facility was selected as the target facility, and five target regions were selected considering the Koppen climate classification system and the C40 Cities Climate Leadership Group. Finally, a total of 5 x 1.01 x 10(22) occupant behavior combinations can be generated in the five target regions. As a result, among the four target variables (i.e., total energy consumption, thermal comfort, IAQ and visual comfort), the total energy consumption of the optimal solution was found to have changed most dramatically compared to that of the basic condition in terms of percentage (94.7%), due to its strong correlation with the overall occupant behavior (the highest correlation coefficient: 0.879). Therefore, it is shown that occupant behavior has more influence on the total energy consumption than on the three IEQ indices. Among the three IEQ indices, the IAQ of the optimal solution decreased most significantly compared to that of the basic condition (the highest reduction ratio: 4.04% in Ulsan), which indicates that the IAQ has more influences on the integrated IEQ score than thermal and visual comfort. The facility manager and the building user can operate the building for reducing total energy consumption and improving the IEQ considering occupant behavior, which can be used as the building management guideline in various regions. (C) 2017 Elsevier Ltd. All rights reserved.</P>

천속단(Phlomis umbrosa Turcz)의 파골세포 분화에 미치는 영향

백종민(Jong Min Baek),박주하(Ju Ha Park),송정훈(Jeong Hoon Song),박성남(Seong-Nam Park),정우진(Woo Jin Jeong),문서영(Seo Young Moon),이창훈(Chang Hoon Lee),최민규(Min-Kyu Choi),김정중(Jeong Joong Kim),오재민(Jaemin Oh),장성조(Sung Jo 대한체질인류학회 2013 해부·생물인류학 (Anat Biol Anthropol) Vol.26 No.3

골다공증, 류마티스 관절염을 비롯한 많은 골질환은 파골세포의 지나친 골 흡수 활동으로 인하여 발생한다. 최근 들어, 천연물에서 추출한 성분을 이용하여 새로운 치료물질을 개발하기 위한 연구가 활발하게 진행되고 있다. 그 중, 천속단이라고 알려진 천연물질은 뼈를 튼튼하게 해주는 효능이 있는 것으로 알려져 있으나, 천속단의 골질환과 관련된 정확한 효과나 치료기전에 대한 정보는 아직 알려진 바가 없다. 따라서 본 연구에서는 골수탐식세포에서 receptor activator of nuclear factor-κB ligand (RANKL)로 유도한 파골세포의 분화와 골 흡수능에 천속단이 어떠한 영향을 미치는지 알아보았다. 또한, 파골세포의 분화와 관련된 작용기전을 규명하였다. 본 실험에서 천속단은 RANKL로 유도한 파골세포의 분화와 골 흡수능을 현저히 억제하였고, RANKL과 macrophage-colony stimulating factor (M-CSF)를 처리한 골수탐식세포에서 NF-κB의 발현을 저해하였다. 더 나아가, c-Fos를 비롯한 nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP)의 mRNA 발현이 천속단에 의해 억제됨을 확인하였으며, 세포융합과 관련 있는 유전자인 Integrin αν, β3와 actin ring에 관여하는 유전자인 dendritic cell-specific transmembrane preotein (DC-STAMP)그리고 골 흡수능과 연관된 Cathepsin K 또한 같은 결과를 나타내었다. 이러한 결과는 천속단의 골다공증을 비롯한 골질환 치료의 후보물질로써의 가능성을 제시하였다. Many bone-related diseases such as osteoporosis, rheumatoid arthritis are occurred by excessive bone resorbing activity of osteoclasts. Recently, many studies have been proceeded to find out the new therapeutic materials from natural products of plants. Phlomis umbrosa Turcz, one of the natural products of plants has been known to improve bone health. However, the precise effects and treatment mechanisms of Phlomis umbrosa Turcz about bone diseases has been unknown. So, we examined the effects of Phlomis umbrosa Turcz on expression of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow-derived macrophages (BMMs) and bone resorption. Also, we investigated the treatment mechanisms of Phlomis umbrosa Turcz relating to osteoclast differentiation. Here, we showed that Phlomis umbrosa Turcz significantly suppressed RANKLinduced osteoclast differentiation and bone resportion. Furthermore, Phlomis umbrosa Turcz suppressed the activation of NF-κB in bone marrow macrophage treated RANKL and M-CSF. The mRNA expression of c-Fos, nuclear factor of activated T-cells (NFAT)c1, osteoclast-associated receptor (OSCAR), tartrate-resistant acid phosphatase (TRAP) in BMMs was inhibited by Phlomis umbrosa Turcz. Integrin αν, β3 relating to cell adhesion and dendritic cell-specific transmembrane protein (DC-STAMP) relating to the structure of filamentous actin (F-actin) ring and cathepsin K relating to bone resorbing activity are disrupted too. These results suggest that Phlomis umbrosa Turcz will be a good materials to treat bone diseases like osteoporosis.

Design and Methodology for the Korean Observational and Escitalopram Treatment Studies of Depression in Acute Coronary Syndrome: K-DEPACS and EsDEPACS

JaeMin Kim,KyungYeol Bae,HeeJu Kang,SungWan Kim,IlSeon Shin,YoungJoon Hong,JuHan Kim,HeeYoung Shin,YoungKeun Ahn,JongKeun Kim,MyungHo Jeong,JinSang Yoon 대한신경정신의학회 2014 PSYCHIATRY INVESTIGATION Vol.11 No.1

Depression is common after acute coronary syndrome (ACS), adversely affecting cardiac course and prognosis. There have been only a few evidence-based treatment options for depression in ACS. Accordingly, we planned the Korean Depression in ACS (K-DEPACS) study, which investigated depressive disorders in patients with ACS using a naturalistic prospective design, and the Escitalopram for DEPACS (EsDEPACS) trial, which assessed the efficacy and safety of escitalopram for treating major or minor depression in patients with ACS. Participants in the K-DEPACS study were consecutively recruited from patients with ACS who were recently hospitalized at Chonnam National University Hospital, Gwangju, South Korea. Diagnoses were confirmed by coronary angiography from 2005. Data on depressive and cardiovascular characteristics were obtained at 2 weeks, 3 months, 12 months, and every 6 months thereafter following the index ACS admission. The K-DEPACS participants who met the DSM-IV criteria for major or minor depressive disorder were randomly assigned to groups in the 24-week, double-blind, placebo-controlled EsDEPACS trial beginning in 2007. The outcome of treatments for depressive and other psychiatric symptoms, issues related to safety, including general adversity, and cardiovascular factors were assessed. The K-DEPACS study can significantly contribute to research on the complex relationships between depression and ACS. The results of the EsDEPACS trial provide an additional treatment option for clinicians treating these patients.

Online Submission and Review System for Open Science: A Case of AccessON Peer Review Management System Plus (ACOMS+)

Jaemin Chung,Eunkyung Nam,Sung-Nam Cho,Jeong-Mee Lee,Hyunjung Kim,Hye-Sun Kim,Wan Jong Kim Korea Institute of Science and Technology Informat 2024 Journal of Information Science Theory and Practice Vol.12 No.1

As the academic publishing environment evolves rapidly and the open science paradigm emerges, the demand for efficient and transparent peer review is growing. This study outlines efforts to actively introduce advanced concepts in scholarly communication into the submission and review system. AccessON Peer Review Management System Plus (ACOMS+), developed and operated by the Korea Institute of Science and Technology Information, is an online submission and peer review system that aims for open science. This study provides an overview of ACOMS+ and presents its four main features: open peer review, open access publishing and self-archiving, online quantitative/qualitative evaluation, and peer reviewer invitation. The directions for further developing ACOMS+ to fully support open science are also discussed. ACOMS+ is the first system in Korea to introduce the open peer review process and is distinguished as a system that supports open access publishing and digital transformation of academic journals. Furthermore, ACOMS+ is expected to contribute to the advancement of the academic publishing environment through the increasing shift toward open access publishing, transparent peer review, and open science.

A CONSERVATIVE NUMERICAL METHOD FOR THE CAHN-HILLIARD EQUATION IN COMPLEX DOMAINS

Jaemin Shin,Darae Jeong,Junseok Kim 한국산업응용수학회 2011 한국산업응용수학회 학술대회 논문집 Vol.6 No.1

We propose an efficient finite difference scheme for solving the Cahn-Hilliard equation with a variable mobility in complex domains. Our method employs a type of unconditionally gradient stable splitting discretization. We also extend the scheme to compute the Cahn-Hilliard equation in arbitrarily shaped domains. We prove the mass conservation property of the proposed discrete scheme for complex domains. The resulting discretized equations are solved using a multi grid method. Numerical simulations are presented to demonstrate that the proposed scheme can deal with complex geometries robustly. Furthermore, the multigrid efficiency is retained even if the embedded domain is present.

Black rice extract protected HepG2 cells from oxidative stress-induced cell death via ERK1/2 and Akt activation

Jaemin Yoon,Hyeonmi Ham,Jeehye Sung,Younghwa Kim,Youngmin Choi,Jeom-Sig Lee,Heon-Sang Jeong,Junsoo Lee,Daeil Kim 대한지역사회영양학회 2014 Nutrition Research and Practice Vol.8 No.2

BACKGROUND/OBJECTIVES: The objective of this study was to evaluate the protective effect of black rice extract (BRE) on tert-butyl hydroperoxide (TBHP)-induced oxidative injury in HepG2 cells. MATERIALS/METHODS: Methanolic extract from black rice was evaluated for the protective effect on TBHP-induced oxidative injury in HepG2 cells. Several biomarkers that modulate cell survival and death including reactive oxygen species (ROS), caspase-3 activity, and related cellular kinases were determined. RESULTS: TBHP induced cell death and apoptosis by a rapid increase in ROS generation and caspase-3 activity. Moreover, TBHP-induced oxidative stress resulted in a transient ERK1/2 activation and a sustained increase of JNK1/2 activation. While, BRE pretreatment protects the cells against oxidative stress by reducing cell death, caspase-3 activity, and ROS generation and also by preventing ERKs deactivation and the prolonged JNKs activation. Moreover, pretreatment of BRE increased the activation of ERKs and Akt which are pro-survival signal proteins. However, this effect was blunted in the presence of ERKs and Akt inhibitors. CONCLUSIONS: These results suggest that activation of ERKs and Akt pathway might be involved in the cytoprotective effect of BRE against oxidative stress. Our findings provide new insights into the cytoprotective effects and its possible mechanism of black rice against oxidative stress.