Enhanced Maturation and Function of Dendritic Cells Using Hydrogel Coated Plate and Antigen Electroporation

Son, Cheol-Hun,Bae, Jae-Ho,Shin, Dong Yeok,Lee, Hong-Rae,Choi, Yung Hyun,Yang, Kwangmo,Park, You-Soo Informa Healthcare 2013 Immunological investigations Vol.42 No.4

<P>Dendritic cells (DCs) are potent antigen-presenting cells that can be matured <I>in vitro</I> from immature dendritic cells (iDCs) in the presence of several biological agents such as cytokine cocktail, CD40L, TNF-a and antigen loading, which are necessary and achieved using various protocols, such as lipofection, passive pulse or electroporation. However, these DCs maturation protocols may cause with a significant loss of cells because of cellular attachment and spreading during culturing. Some biomaterials that influence adhesion and development of cells have been used in cell culture techniques, and it was thought that they might be applied on the culture of DCs. In this study, we used polyHEMA, which is a hydrogel coating biomaterial that prevents DCs from adherence, and investigated whether hydrogel coating affects the maturation of iDCs. The efficiency in the generation of mDCs was improved through hydrogel coating procedure and a dendritic cell maturation marker, CD83, was significantly increased in hydrogel-coated culture condition. The antigen-loaded mDCs from electroporation were further expressed the CD83. The mDCs generated in the hydrogel-coated culture condition showed more, longer and thicker dendrites, and produced more amounts of cytokines such as IL-12 and IFN-γ. Therefore, it was suggested that the hydrogel-coated culture condition could improve function of mDCs.<fn><P>Cheol-Hun Son and Jae-Ho Bae contributed equally to this work.</P></fn></P>

일관성 광대역 정합장처리에 의한 수중 이동음원의 위치추적

신기철,박재은,김재수 韓國海洋大學校 附設 海洋科學技術硏究所 2002 硏究論文集 Vol.11 No.2

최근 수중음향에 대한 관심은 천해와 같은 복잡한 음파의 상호작용과 경계조건이 부정확한 해양환경으로 이동하고 있다. 천해 음향전파에 의해서 발생하는 어려움을 극복하기 위한 방법으로 음원의 광대역 스펙트럼에서 얻어지는 정보를 사용하여 견고성과 신뢰성을 증가시키는 광대역 정합장처리 기법이 사용된다. 본 논문에서는 협대역에서의 음장의 공간 일관성을 확장하여 다중 주파수를 사용한 주파수-공간 일관성 광대역 프로세서를 제안하였고, 이 프로세서를 실제 광대역 이동음원에 적용하여 음원위치추적을 수행하였다. 제안된 일관성 프로세서는 음원의 정위치 추정확률이 높았으며, 특히 정합장출력의 부엽준위에서 비일관성 프로세서와 비교해 일관성 프로세서가 상당히 낮은 준위를 나타내었다. The shallow-water environment presents additional challenges arising from the complex interaction patterns of the sound with the sea bed. In order to overcome the difficulties generated by shallow-water propagation, broad-band matched field processing has been employed in an effort to increase robustness by utilizing multiple frequency information. In this paper, a coherent broad-band matched field processor is introduced that incorporates the spatial coherence of the acoustic field not only over one frequency but across frequencies. The incoherent and coherent processors are applied to the experimental data where it is shown that both processors give a high probability of correct localization. Also it is found that a coherent processor has better performance in the sidelobe pattern of ambiguity surfaces.

입원한 입시 스트레스 증후군 환자의임상양상에 관한 정신의학적 연구

신광철,진태원,이영식,이재우,이재광,이길홍 중앙대부설한국의과학연구소 1991 한국의과학 Vol.23 No.3

대학관리운영의 전문화

신재철 全南大學校 人文科學硏究所 1990 용봉인문논총 Vol.19 No.-

두부외상에 의한 미만성 축삭손상의 임상적 병리학적 관찰

신영귀,이민철,이영직,박창수,김재휴,박문선 대한신경외과학회 1997 Journal of Korean neurosurgical society Vol.26 No.6

기하광학영역에서의 능동소나 표적신호합성

신기철,박재은,김재수,최상문,김우식 한국해양대학교 해양과학기술연구소 2001 硏究論文集 Vol.10 No.1

능동소나 시스템에서 표적신호의 근거리 정보가 중요해짐에 따라, 표적 음향산란 신호의 고유한특성을 분석하기 위해 실험적인 방법과 시뮬레이션 기법이 사용된다. 본 논문에서는 표적신호 합성을 위해 축소표적 반향실험을 수행하였고, 자료의 분석 결과 축소표적의 음향산란은 거울면 반사의 영향이 큰 것으로 분석되어, 표적신호 합성에서 기하광학이론을 응용하였다. 복잡한 형태의 표적을 여러 표본형상으로 분리하고, 각 표본형상의 기여도를 신호의 위상과 강도를 줌으로써 계산하였으며. 표적신호 합성모델의 검증을 위해 실제 축소표적 실험 결과와 비교하였다. 혁심용어: 기하광학이론. 음향산란, 거울면반사. 축소표적, 능동소나 투고분야: 수중음향 분야 (5.2) Since the near field iformation of target signal is important in the development and verification of active sonar system experimental method and simulation technique are widely used in order to analyze the detail characteristics of target scattered echoes. Therefore, in this paper, the scale target experiment is performed to develope and improve the target signal simulation model. Since the experimental results show that the specular reflection is the major component among scattering mechanisms, the target signal simulation model based on the Geometric Optics Theory (GOT) is developed. Complex target is separated into simple shapes, known as canonical shape. The contribution from individual canonical shapes are summed with proper phase and amplitude to produse the target strength of the whole complex body. Simulated target signal is compared with the experimental results fed discussed.

R.F. Magnetron Sputtering법으로 제조한 LaCoO₃박막의 가스감지 특성

신정호,김정규,박기철,전춘배,장재영 慶尙大學校 工科大學 自動化및컴퓨터應用技術硏究所 1998 自動化 및 컴퓨터應用技術 Vol.5 No.1

LaCoO₃(LCO) thin film sensors were fabricated on an alumina substrate by r.f. magnetron sputtering. By varying the annealing temperature of the LCO film from 600℃ to 1000℃, we investigated X-ray diffraction pattern, electrical properties, and gas sensing properties of the films. The crystallinity was improved and the activation energy was increased with increasing the annealing temperature. The sensitivities of the LCO films for NH₃ and CO gases were also increased with the annealing temperature. Sensing properties of LCO thin films were improved when the measuring temperature was 350℃ or more

입원한 정신장애자의 도박성향에 관한 연구

신광철,이길홍,이재광 大韓神經精神醫學會 1991 신경정신의학 Vol.30 No.5

The authors have tried this study to know gambling trends, to extract various factors that can be related to gambling behaviors of the mentally illed persons, and to make the development of preventive guidelines which can prevent gambling behaviors in advance. Samples were gathered from 428 psychiatric inpatients who were admitted to hospitals in Seoul and district on March 1988. Data were processed by IBM PC AT Computed using SPSS+ program and were statistically analysed by internal consistency of gambling items, correlation matrix of gambling items and chi-square test. Summary of the results were as follows: 1) Among the nine diagnostic criteria for pathological gambling, three diagnostic criteria, such as “restlessness or irritability if unable to gamble(22.2%)”,“frequent gambling of larger amounts of money or over a over a longer period of time than intended(20.6%)”,and“a need to increase the size or frequency of bets to achieve the desired excitement(20.3%)”,were more frequently experienced criteria. And all the nine criteria were experienced more frequently in males than in females. 2) 32.7% (140 persons) of the total psychiatric inpatients(428 persons), including social gambler as 13.1% and pathological gambler as 19.6%, have experienced gambling behaviors. The rate of pathological gambling in males as 26.1% was 2.46 times higher than females 10.6%. 3) Pathological gambling trends were highest in psychiatric inpatients who has grown up in the outskirts(25.7%) and the center of the small and medium city(22.4%), and higher in the outskirts of the Seoul(21.9%) and the center of the large city(21.1%), but pathological gambling trends were lowest in the group who has grown up in center of Seoul. But gambling trends were not correlated with age level, educational level, and location of the hospital. 4) Pathological gambling trends were higher in psychiatric inpatients whose body types dysplastic(52.2%), while lower in balanced body type(14.3%). 5) Pathological gambling trends were higher in psychiatric inpatients whose personality trait is antisocial(50.0%) than the other personality trait, while lower in borderline personlity (2.9%). 6) Pathological gambling trends were higher in psychiatric inpatients who have learned masturbation during middle adolescent period and who had difficulties in premorbid sexual adjustment. 7) Gambling trends were higher in psychiatric inpatients who drinks alcohol habitually (Social drinking, 36.4%; alcohol addiction, 32.7%). Among them, gambling trends were higher in patients who drink alcohol hedonically(30.1%). However gambling trends were lower in patients who do not drink(9.7%).

Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.

Mo¨ssbauer Spectrometer의 속도변환 장치의 제작

申榮男,朴成培,金在慶,裵錫喆 大邱大學校附設 基礎科學硏究所 1991 基礎科學硏究 Vol.8 No.-

A constant acceleration Mossbauer spectrometer is constructed and a reference signal of the desired waveform to drive the feedback system is produced by the multichannel scaling board and with the help of operational amplifier. The stability and the linearity of the system for a triangular velocity waveform is investigated in the measurement of Mossbauer spectra of standard sample(αFe, αFe₂O₃, stainless steel and Na₂[Fe(CN)_(5)NO]₂H₂O). The system is stabilized and the linearity of the system for triangular velocity waveform is better than ?0.01% for the half-period of 14mm/sec velocity.