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      • Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection

        Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3

        Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.

      • KCI등재

        Simultaneous subtyping and pathotyping of the novel reassortant influenza A (H5N8) virus from clinical samples using a diagnostic microarray

        Kwon, J. H.,Kim, J. H.,Lee, D. h.,Cho, H.,Hwang, S. Y.,Yuk, S. S.,Erdene-Ochir, T. O.,Noh, J. Y.,Hong, W. T.,Jeong, J. H. Springer Science + Business Media 2016 BioChip Journal Vol.10 No.3

        <P>Highly pathogenic avian influenza (HPAI) viruses cause economic losses in the poultry industry and pose a severe threat to human health. Rapid and accurate diagnostic methods are important because they can help prevent further spread of the virus and reduce the time required for eradication of the virus. We developed a low-density microarray for the rapid detection and identification of avian influenza virus subtypes H5, H7, and H9 and their pathotypes in a previous study. In the present study, we report the development of updated probe sets and evaluation of the diagnostic microarray using H5N8 clade 2.3.4.4 HPAI viruses including clinical samples, without the need for egg propagation. Cy3-labeled DNA targets were obtained by reverse transcription polymerase chain reaction using Cy3-labeled universal primers, and labeled amplicons were hybridized to the microarray. All positive samples from RT-PCR showed H5-specific and highly pathogenic pattern in the microarray, without purification of PCR products. Furthermore, it allowed for specific detection of the subtype and pathotype from low DNA concentration samples that did not allow direct sequence analysis. Therefore, this diagnostic microarray has enormous potential for the rapid subtyping and pathotyping from clinical samples without the need for culture.</P>

      • Si film electrodes with surface-modified Cu current collectors for micro Li batteries

        Lee, M.j.,Chae, M.r.,Jeong, J.s.,Noh, J.p.,Ahn, H.j.,Cho, K.k.,Choi, H.k.,Nam, T.h.,Kim, K.w.,Cho, G.b. Pergamon Press 2016 Materials research bulletin Vol.82 No.-

        <P>Si film electrodes were fabricated onsurface-modified Cu current collectors using an oxidation-reduction process. Flower-like nanostructures (FLNSs) with diameters of 2-3 mu m and plate-like nanostructures (PLNSs) with lengths of 1 m were formed on the Cu foil oxidized at 423 K for 0.5 h, but only the PLNSs remained after sonication. Reduction of the preoxidized Cu foil at 673 K resulted in the formation of platelike and coral -like nanostructures on the Cu foils reduced for 1 and 3 h and a smooth surface without specific structures on the Cu foil reduced for 6 h. The best electrochemical properties in terms of the first columbic efficiency (85.4%) and the cycle performance (67.3% at 50 cycles) were obtained from the Si film electrode fabricated on the Cu foil that had been reduced for 3 h because the coral -like nanostructures on the Cu foil enhanced the adhesion of the Si film and improved the structural stability of the Si film electrode during the electrochemical reactions. (C) 2016 Elsevier Ltd: All rights reserved.</P>

      • KCI등재SCIESCOPUS

        Investigation of the electrical and optical properties of InAs/InGaAs dot in a well solar cell

        Lee, S.H.,Han, I.S.,Sohn, C.W.,Jo, H.J.,Kim, J.S.,Lee, S.J.,Noh, S.K.,Kim, J.O. Elsevier 2015 Current Applied Physics Vol.15 No.11

        The electroreflectance (ER) and current-voltage (J-V) of InAs/InGaAs dots in a well (DWELL) solar cell (SC) were measured to examine the optical and electrical properties. To investigate the carrier capturing and escaping effects in the quantum dot (QD) states the above and below optical biases of the GaAs band gap were used. In the reverse bias region of the J-V curve, the tunneling effect in the QD states was observed at low temperature. The ideality factors (n) were calculated from the J-V curves taken from various optical bias intensities (I<SUB>ex</SUB>). The changes in the ideality factor (n) and short circuit current (J<SUB>SC</SUB>) were attributed mainly to carrier capture at low temperature, whereas the carrier escaping effect was dominant at room temperature. ER measurements revealed a decrease in the junction electric field (F<SUB>J</SUB>) due to the photovoltaic effect, which was independent of the optical bias source at the same temperature. At low temperature, the reduction of photovoltaic effect could be explained by the enhancement carrier capturing effect due to the strong carrier confinement in QDs.

      • SCISCIESCOPUS

        Efficacy of clade 2.3.2 H5 commercial vaccines in protecting chickens from clade 2.3.4.4 H5N8 highly pathogenic avian influenza infection

        Yuk, S.s.,Erdene-Ochir, T.O.,Kwon, J.H.,Noh, J.Y.,Hong, W.t.,Jeong, J.H.,Jeong, S.,Gwon, G.B.,Shin, J.i.,Sur, J.H.,Song, C.S. Butterworths ; Elsevier Science Ltd 2017 Vaccine Vol.35 No.9

        Emerging clade 2.3.4.4 of the highly pathogenic avian influenza (HPAI) virus strain H5N8, which had been detected sporadically in domestic poultry in China, started to affect wild birds and poultry in South Korea in 2014. The virus was spread to Germany, Italy, the Netherlands, United Kingdom, and even United States by migratory birds. Here, we tested currently used commercial clade 2.3.2 H5 vaccines to evaluate mortality, clinical signs, virus shedding, and histological damage after experimental infection of chickens with the clade 2.3.4.4 HPAI H5N8 virus. Although the vaccination protected chickens from death, it failed to prevent chickens from shedding the virus and from tissue damage according to histological examination. These results suggest that the use of appropriate vaccines that match the currently epidemic HPAI virus is recommended, and continuous HPAI surveillance and testing of currently used commercial vaccines should be performed.

      • KCI등재

        Quasi-Monoenergetic Electron-Beam Generation Using a Laser Accelerator for Ultra-Short X-ray Sources

        J Kim,고도경,석희용,H Jang,김형택,I Hwang,최일우,J Lim,J. Lee,J. H. Sung,K.-H. Hong,허민섭,N Hafz,유승훈,유태준,T. M. Jeong,V Kulagin,Y.-C. Noh 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.I

        Two types of electron acceleration methods have been conducted to generate quasi-monoenergetic electron beams. Multi-MeV quasi-monoenergetic high-charge electron beams were generated at Korea Electrotechnology Research Institute (KERI) from self-modulated laser wakefield acceleration by using a collimator with a 2 TW (1.4 J/700 fs) Nd:glass/Ti:sapphire hybrid laser system and a supersonic nitrogen gas jet. The peak electron energy was 3.6 MeV, and the energy spread was 4 MeV. These electron beams are useful for the generation of short-pulse X-rays in the water window region, which is 250 eV -- 500 eV (2.5 -- 5 nm), by using Thomson scattering. The calcualted photon spectrum indicates the scattered photon covers the water window region. This can be used for a high spatial and temperal resolution microscope for medical imaing. To generate higher-energy electron beams with small energy spread, a laser wakefield acceleration experiment with a sharp downward electron density gradient was conducted with a 100 TW laser system at Advanced Photon Research Insistitute (APRI). With the electron density gradient, some background plasma electrons could be locally injected in the laser wake wave and a small energy spread was expected. Using the pre-pulse, we could generate sharp downward electron density gradients. The gradient scale length was 20 $\mu$m for a 25 \% density change. With this electron density gradient, we could get more reproducible electron beams than we could without the density gradient.

      • Organic solar cells using plasmonics of Ag nanoprisms

        Noh, H.S.,Cho, E.H.,Kim, H.M.,Han, Y.D.,Joo, J. Elsevier Science 2013 Organic electronics Vol.14 No.1

        We demonstrate plasmonic effects in bulk heterojunction organic solar cells (OSCs) consisting of poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl-C<SUB>61</SUB>-butyric acid methyl ester (PCBM) by incorporating silver (Ag) triangular shaped nanoparticles (nanoprisms; NPSs) into a poly(3,4-ethylenedioxythiophene) buffer layer. The optical absorption and geometric characteristics of the Ag NPSs were investigated in terms of their tunable in-plane dipole local surface plasmon resonance (LSPR) bands. The photovoltaic characteristics showed that the power conversion efficiency (PCE) of the plasmonic OSCs was enhanced by an increase of short circuit current (J<SUB>sc</SUB>) compared to that of the reference cells without any variation in electrical properties. The enhanced J<SUB>sc</SUB> is directly related to the enhancement of optical absorption efficiency by the LSPR of the Ag NPSs. We measured the photovoltaic characteristics of the plasmonic OSCs with various distances between the Ag NPSs and the P3HT:PCBM active layer, in which the PCEs of the plasmonic OSCs decreased with increasing distance. This suggests that the increase of photocurrent and optical absorption was due to near field enhancement (i.e., intensified incident light on the active layer) by the LSPR of the Ag NPSs.

      • 여중생의 외모만족도와 정신건강의 관계

        노희경,문소현,박세은,서재은,이수현,이현영,임영신,정유리,황유리 이화여자대학교 간호과학대학 2013 이화간호학회지 Vol.- No.47

        Purpose: The purpose of this study was to identify the relationship between satisfaction toward appearance and the level of mental health among female middle school students. Methods: The total study sample was 432 consisting of 3rd year female students who attended co-educational or girls’ middle schools in Korea. Data were collected from August 8 to August 16, 2012. Statistical analyses were conducted using t-test and Pearson’s Correlation Coefficient with SPSS18.0Windows software. Results: There was correlation between appearance satisfaction and mental health, especially with personal sensitivity (r= -.447, p= .000) and depression (r= -.350, p= .000). The level of mental health significantly differed according to type of school (t= -2.668, p= .008). However, appearance satisfaction did not differ according to type of school. Conclusion: The results of this study implied the importance of appropriate education and nursing intervention which would improve satisfaction toward the appearance of female middle school students.

      • KCI등재

        자발형성 InAs/GaAs 양자점의 구조 및 성장 특성

        김형석,서주형,박찬경,이상준,노삼규,송진동,박용주,이정일 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.3

        Self-assembled InAs/GaAs quantum dots (QDs) were grown by molecular beam epitaxy and the growth characteristics of QDs were studied using field emission gun-electron transmission microscope. The shapes and optical properties of QDs changed according to spacer layers thickness due to the strain between InAs and GaAs layers. The QDs with 50 nm thick GaAs spacer layers were distributed randomly along the growth direction and changed from dome to flat-pyramidal shape after capping with GaAs. However, QDs with 10 nm thick spacer layers were vertically aligned up to the fifth period and the dome-shape was maintained after capping. The density, distribution and crystalline defects depending on growth conditions were also investigated.

      • KCI등재

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