Hypoxia-induced PLOD2 regulates invasion and epithelial-mesenchymal transition in endometrial carcinoma cells

Junhui Wan,Junli Qin,Qinyue Cao,Ping Hu,Chunmei Zhong,Chunhua Tu 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 (PLOD2) was induced in hypoxia and participated in cancer development. However, the role of PLOD2 in endometrial carcinoma remains unclear. Objective To explore the influences and regulation mechanism of PLOD2 in endometrial carcinoma under hypoxic condition. Methods The small interfering RNA (siRNA) targeting to PLOD2 and pcDNA3.1-PLPD2 were transfected to endometrial carcinoma cells to alter PLOD2 expression. Cell proliferation ability was determined by colony formation assay. Wound healing assay used to detect cell migration ability. Transwell invasion assay was used to detect cell invasion ability. Results PLOD2 and Hypoxia-inducible factor-1α (HIF-1α) were induced by hypoxia. Down-regulation of PLOD2 did not affect endometrial carcinoma cell proliferation ability, while inhibited cell migration, invasion under hypoxic condition. Besides, down-regulation of PLOD2 increased the levels of γ-catenin and E-cadherin and decreased levels of Fibronectin and Snail under hypoxic condition. Down-regulation of PLOD2 also inactivated Src and phosphoinositide 3-kinase (PI3K)/ protein kinase B (Akt) signaling under hypoxic condition. The promoting effects of PLOD2 overexpression on migration, invasion and epithelial-mesenchymal transition (EMT) of endometrial carcinoma cells were reversed by Akt inhibitor (MK2206) under hypoxic condition. Conclusion PLOD2 expression was increased in endometrial carcinoma cells under hypoxic condition. PLOD2 modulated migration, invasion, and EMT of endometrial carcinoma cells via PI3K/Akt signaling. PLOD2 may be a potential therapeutic target for endometrial carcinoma.

회수수에 당류계 안정화제를 처리한 시멘트 모르터의 특성

金虎林,우종완,한민철,류현기,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

In this paper, fundamental properties of cement mortar using recycled water with stabilizing agent are discussed in order to investigate the applicability of recycled water. Stabilizing agent content, solid contents and open time are varied in this paper. For fresh mortar properties, highest fluidity with 0.15% of stabilizing agent was observed and air content was increased with an increase in stabilizing agent contents. Solid contents and open time of recycled water had nothing to do with fluidity and air content loss. Little difference in setting time was found with the presence of stabilizing agent and without, while it was accelerated with an increase in open time and solid content. For strength properties, the highest strength value was observed at 0.15% of stabilizing agent. An increase in solid contents resulted in an enhancement of strength, while strength decreased with an increase in open time and elapse of age. Drying shrinkage exhibited a reduction with an increase in stabilizing agent contents and open time. From the present paper, the combination of 0.15% of sacharic based stabilizing agent, 3% of solid content and 1day of open time is found to be an optimal condition to perform effectively.

회수수에 처리되는 당류계 안정화제의 혼입률 변화가 콘크리트의 품질에 미치는 영향

文學龍,金虎林,우종완,한민철,한천구 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The objective of present paper is to investigate the engineering properties of concrete using recycling water incorporating saccharic based stabilizing agent in order to improve qualities of concrete in the region of normal strength and high strength. According to test results, fluidity and air content do not exhibit significant difference with an increase in stabilizing agent contents. Bleeding was not observed at 30% of W Ie, regardless of stabilizing agent contents, nonetheless, concrete with stabilizing agent of 0.15% was observed to occur the least bleeding at 40 and 50% of W IC. For setting time, although presence of recycled water accelerates setting time, setting time shows to be retarded gradually with an increase in stabilizing agent content. For compressive strength, use of stabilizing agent enhanced compressive strength of concrete compared with that of concrete without stabilizing agent. In the side of strength enhancement, adequate dosage of stabilizing agent is found to be 0.15%. As dosage of stabilizing agent increased above 0.15%, drying shrinkage exhibited a tendency to decrease.

Seismic Response and Evaluation of SDOF Self-Centering Friction Damping Braces Subjected to Several Earthquake Ground Motions

Hu, Jong Wan,Noh, Myung-Hyun Hindawi Limited 2015 Advances In Materials Science And Engineering Vol.2015 No.-

<P>This paper mainly deals with seismic response and performance for self-centering friction damping braces (SFDBs) subjected to several maximum- or design-leveled earthquake ground motions. The self-centering friction damping brace members consist of core recentering components fabricated with superelastic shape memory alloy wires and energy dissipation devices achieved through shear friction mechanism. As compared to the conventional brace members for use in the steel concentrically braced frame structure, these self-centering friction damping brace members make the best use of their representative characteristics to minimize residual deformations and to withstand earthquake loads without member replacement. The configuration and response mechanism of self-centering friction damping brace systems are firstly described in this study, and then parametric investigations are conducted through nonlinear time-history analyses performed on numerical single degree-of-freedom spring models. After observing analysis results, adequate design methodologies that optimally account for recentering capability and energy dissipation according to their comparative parameters are intended to be suggested in order to take advantage of energy capacity and to minimize residual deformation simultaneously.</P>

Molecular cloning and characterization of a short peptidoglycan recognition protein (PGRP-S) with antibacterial activity from the bumblebee Bombus ignitus

Hu Wan,Kwang Sik Lee,Bo Yeon Kim,Hyung Joo Yoon,Hung Dae Sohn,Byung Rae Jin 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Peptidoglycan recognition proteins (PGRPs) are pattern recognition molecules of the innate immune system that recognize peptidoglycan, a unique cell wall component of bacteria. Here we cloned and characterized PGRP-S from the bumblebee Bombus ignitus (BiPGRP-S). The BiPGRP-S gene consists of four exons encoding 194 amino acid residues. Comparative analysis indicates that the predicted amino acid sequence of BiPGRP-S shares high identity with enzymatically active PGRP-S proteins and contains the amino acids required for amidase activity. BiPGRP-S in B. ignitus worker bees is constitutively expressed in boththe fat body and epidermis, and it is secreted into the hemolymph. Quantitative real-time PCR assays revealed that in both the fat body and epidermis, the BiPGRP-S gene is highly induced by an injection of Bacillus thuringiensis. In addition, recombinant BiPGRP-S expressed as a 19-kDa protein in baculovirus-infected insect cells can bind to B. megaterium and B. thuringiensis but not to Staphylococcus aureus, Escherichia coli or Beauveria bassiana. Consistent with these data, BiPGRP-S shows antibacterial activity against B. megaterium and B. thuringiensis. These results indicate that BiPGRP-S is an inducible protein that may be involved in the immune response against bacterial infection of the genus Bacillus as an amidase-type PGRP-S.

Analytical Models for Determining Rotational Capacity of Clip-Angle Connections under Seismic Loading

Hu, Jong Wan,Son, Hong Min Trans Tech Publications 2014 Advanced materials research Vol.1025 No.-

<P>This paper explores rotational capacities and demands in thick top-and-seat (cleated) angle partially restrained connections subjected to monotonic and cyclic loads. The results of test on full-scale angle connections are described first, and are then compared to published curve-fitting models for these types of connections. The data indicates that the curve-fitting constants of some existing mathematical models cannot be extrapolated to thick angles. The results indicate that these connections are capable of providing very ductile behavior and constitute an ideal back-up structural system in steel frames.</P>

Towards an Application of PBD Principles for Innovative Recentering Beam-to-Column Connections

Hu, Jong Wan,Choi, Ga Lam Trans Tech Publications, Ltd. 2013 Advanced materials research Vol.716 No.-

<P>An unintended consequence is that some freedom in the introduction of innovative composite connections has been removed. The innovative aspects of this connection are in the use of partial restraint connections between steel beams and concrete-filled tube columns that utilize a combination of low-carbon steel and shape memory alloy components. A refined finite element model with sophisticated three dimensional solid elements was developed to conduct numerical experiments on the proposed joints to obtain the global behavior of the connection and develop simplified models. The paper argues that careful analytical studies can replace the requirement for physical testing present in current steel codes.</P>

Effect of the Secondary Phase on Mechanical and Damping Properties of Mg–Zn–Y–Si Alloy

Wan Diqing,Wang Houbin,Hu Jiajun,Hu Yinglin,Wang Linsen,Fang Kangjin 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.5

The Mg–Zn–Y–Si alloy containing the secondary phase is designed by conventional casting. The Mg2Siphase with differentvolume fractions is obtained by controlling the content of Si (mass fraction: 0%, 0.3%, 1.0%, 3.0%). The effects of Mg2Siphase and LPSO phase on the mechanical properties and damping properties of the alloys are investigated. The results showthat due to the addition of Si, the grain of the alloy is refined, and the intergranular secondary phase Mg2Siphase is created.In addition, the addition of Si also causes the bulk LPSO phase inherent in the matrix to transform into a thin-strip LPSOphase, which greatly improves the mechanical properties of the alloys. The damping properties of the alloys have also beenimproved due to the new internal friction mechanisms in the alloy. Therefore, the overall performance of mechanical anddamping of the alloy is greatly improved.

Molecular cloning and characterization of a diapause-specific peptide in the beet armyworm, Spodoptera exigua

Hu Wan,Miao Yuan,Hong You,Jianhong Li,진병래 한국응용곤충학회 2012 Journal of Asia-Pacific Entomology Vol.15 No.3

The leaf beetle, Gastrophysa atrocyanea, diapause-specific peptide (DSP), which plays a role in diapause,inhibits Ca2+ channels and has antifungal activity. Here, we show the molecular cloning and characterization of a diapause-specific peptide in the beet armyworm Spodoptera exigua. The S. exigua diapause-specific peptide (SeDSP) gene consists of only one exon encoding 63 amino acid residues. A comparative analysis showed that mature SeDSP consists of 40 amino acid residues including six cysteines, which are similar to those of S. littoralis Spodomicin and G. atrocyanea DSP. The SeDSP was expressed as a 4.5 kDa peptide in baculovirusinfected insect cells. SeDSP was constitutively expressed in the epidermis of S. exigua larvae and pupae after molting and metamorphosis. In addition, recombinant SeDSP showed antibacterial activity against Bacillus megaterium

Design and Control of Tapping Mode Atomic Force Microscope in Liquid Utilizing Optical Pickup System

Wan-Lin Hu,Shao-Kang Hung,Li-Chen Fu 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10

In this work, we propose a fluid tapping mode atomic force microscopy (AFM) implemented by a DVD pickup head. The use of DVD pickup head minimizes the volume of the hardware system, and thus reduces the measurement error caused by heat expansion. In order to realize the system mentioned above, we design a Q-controller to modulate the interaction force between the tip and the sample. Increasing the quality factor will overcome the problem with high damping ratio in the fluid which makes the probe hard to oscillate. Because of the reduction of the tip-sample force, the sample surface will not be hurt by the tip. Therefore, we can use the AFM to scan soft sample, and obtain more realistic topography. Traditionally, people use proportion-integration controller to control the system. Users need to tune this kind of controller manually, and hence the quality of the scan images is highly related to users’experiences. To overcome this problem, we design an adaptive sliding-mode controller to improve the scanning capability and robustness.For testing the system capability, we will have a series of numerical simulations.