Radixin Knockdown by RNA Interference Suppresses Human Glioblastoma Cell Growth in Vitro and in Vivo

Qin, Jun-Jie,Wang, Jun-Mei,Du, Jiang,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Radixin, a member of the ERM (ezrin-radixin-moesin) family, plays important roles in cell motility, invasion and tumor progression. It is expressed in a variety of normal and neoplastic cells, including many types of epithelial and lymphoid examples. However, its function in glioblastomas remains elusive. Thus, in this study, radixin gene expression was first examined in the glioblastoma cells, then suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method.We found that there were high levels of radixin expression in glioblastoma U251cells. Radixin shRNA caused down-regulation of radixin gene expression and when radixin-silenced cells were implanted into nude mice, tumor growth was significantly inhibited as compared to blank control cells or nonsense shRNA cells. In addition, microvessel density in the tumors was significantly reduced. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin- suppressed glioblastoma U251 cells. In contrast, MMP9 was down-regulated. Taken together, our findings suggest that radixin is involved in GBM cell migration and invasion, and implicate TSP-1, E-cadherin and MMP9 as metastasis-inducing factors.

Mini-Array of Multiple Tumor-associated Antigens (TAAs) in the Immunodiagnosis of Esophageal Cancer

Qin, Jie-Jie,Wang, Xiao-Rui,Wang, Peng,Ren, Peng-Fei,Shi, Jian-Xiang,Zhang, Hong-Fei,Xia, Jun-Fen,Wang, Kai-Juan,Song, Chun-Hua,Dai, Li-Ping,Zhang, Jian-Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Sera of cancer patients may contain antibodies that react with a unique group of autologous cellular antigens called tumor-associated antigens (TAAs). The present study aimed to determine whether a mini-array of multiple TAAs would enhance antibody detection and be a useful approach in esophageal cancer detection and diagnosis. Our mini-array of multiple TAAs consisted of eleven antigens, p53, pl6, Impl, CyclinB1, C-myc, RalA, p62, Survivin, Koc, CyclinD1 and CyclinE full-length recombinant proteins. Enzyme-linked immunosorbent assays (ELISA) were used to detect autoantibodies against eleven selected TAAs in 174 sera from patients with esophageal cancer, as well as 242 sera from normal individuals. In addition, positive results of ELISA were confirmed by Western blotting. In a parallel screening trial, with the successive addition of antigen to a final total of eleven TAAs, there was a stepwise increase in positive antibody reactions. The eleven TAAs were the best parallel combination, and the sensitivity and specificity in diagnosing esophageal cancer was 75.3% and 81.0%, respectively. The positive and negative predictive values were 74.0% and 82.0%, respectively, indicating that the parallel assay of eleven TAAs raised the diagnostic precision significantly. In addition, the levels of antibodies to seven antigens, comprising p53, Impl, C-myc, RalA, p62, Survivin, and CyclinD1, were significantly different in various stages of esophageal cancer, which showed that autoantibodies may be involved in the pathogenesis and progression of esophageal cancer. All in all, this study further supports our previous hypothesis that a combination of antibodies might acquire higher sensitivity for the diagnosis of certain types of cancer. A customized mini-array of multiple carefully-selected TAAs is able to enhance autoantibody detection in the immunodiagnosis of esophageal cancer and autoantibodies to TAAs might be reference indicators of clinical stage.

Prognostic Factors Influencing Clinical Outcomes of Malignant Glioblastoma Multiforme: Clinical, Immunophenotypic, and Fluorescence in Situ Hybridization Findings for 1p19q in 816 Chinese Cases

Qin, Jun-Jie,Liu, Zhao-Xia,Wang, Jun-Mei,Du, Jiang,Xu, Li,Zeng, Chun,Han, Wu,Li, Zhi-Dong,Xie, Jian,Li, Gui-Lin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3

Malignant glioblastoma multiforme (GBM) is the most malignant brain tumor and despite recent advances in diagnostics and treatment prognosis remains poor. In this retrospective study, we assessed the clinical and radiological parameters, as well as fluorescence in situ hybridization (FISH) of 1p19q deletion, in a series of cases. A total of 816 patients with GBM who received surgery and radiation between January 2010 and May 2014 were included in this study. Kaplan-Meier survival analysis and Cox regression analysis were used to find the factors independently influencing patient progression free survival (PFS) and overall survival (OS). Age at diagnosis, preoperative Karnofsky Performance Scale (KPS) score, KPS score change at 2 weeks after operation, neurological deficit symptoms, tumor resection extent, maximal tumor diameter, involvement of eloquent cortex or deep structure, involvement of brain lobe, Ki-67 and MMP9 expression level and adjuvant chemotherapy were statistically significant factors (p<0.05) for both PFS and OS in the univariate analysis. Cox proportional hazards modeling revealed that age ${\leq}50$ years, preoperative KPS score ${\geq}80$, KPS score change after operation ${\geq}0$, involvement of single frontal lobe, deep structure involvement, low Ki-67 and MMP9 expression and adjuvant chemotherapy were independent favorable factors (p<0.05) for patient clinical outcomes.

The prognostic significance of estrogen and progesterone receptors in grade I and II endometrioid endometrial adenocarcinoma: hormone receptors in risk stratification

Jun Guan,Liying Xie,Xuezhen Luo,Bingyi Yang,Hongwei Zhang,Qin Zhu,Xiaojun Chen 대한부인종양학회 2019 Journal of Gynecologic Oncology Vol.30 No.1

Objectives: Although patients with grade I and II endometrioid endometrial adenocarcinoma (EEA) are considered with good prognosis, among them 15%–25% died in 5 years. It is still unknown whether integrating estrogen receptor (ER) and progesterone receptor (PR) into clinical risk stratification can help select high-risk patients with grade I–II EEA. This study was to investigate the prognostic value of ER and PR double negativity (ER/ PR loss) in grade I–II EEA, and the association between ER/PR loss and The Cancer Genome Atlas (TCGA) classification. Methods: ER and PR were assessed by immunohistochemistry on hysterectomy specimens of 903 patients with grade I–II EEA. ER and PR negativity were determined when <1% tumor nuclei were stained. Gene expression data were obtained from the TCGA research network. Results: Compared with ER or PR positive patients (n=868), patients with ER/PR loss (n=35) had deeper myometrial infiltration (p=0.012), severer FIGO stage (p=0.004), and higher rate of pelvic lymph node metastasis (p=0.020). In univariate analysis, ER/PR loss correlated with a shorter progression-free survival (PFS; hazard ratio [HR]=5.25; 95% confidence interval [CI]=2.21–12.52) and overall survival (OS; HR=7.59; 95% CI=2.55–22.60). In multivariate analysis, ER/PR loss independently predicted poor PFS (HR=3.77; 95% CI=1.60–10.14) and OS (HR=5.56; 95% CI=1.37–22.55) for all patients, and poor PFS for patients in stage IA (n=695; HR=5.54; 95% CI=1.28–23.89) and stage II–IV (n=129; HR=5.77; 95% CI=1.57–21.27). No association was found between ER/PR loss and TCGA classification. Conclusion: Integrating ER/PR evaluation into clinical risk stratification may improve prognosis for grade I–II EEA patients.

Effects of Isosorbide on the Microphase Separation and Properties of Waterborne Polyurethane Coatings

Jun Hu,Can Tao,Aining Yuan,Junjie Bao,Qin Cheng,Gewen Xu,Yiping Huang 한국고분자학회 2019 폴리머 Vol.43 No.2

Series of waterborne polyurethanes (WPUs) containing poly(tetra methylene glycol) (PTMG), 4,4-dicyclohexylmethane diisocyanate (HMDI), 2,2-dimethylolpropionic acid (DMPA), 2,2,4-trimethyl-1,3-pentanediol (TMPD), isosorbide (ISO) and ethylenediamine (EDA) were synthesized. The effects of ISO on the structure and properties of WPUs were studied by gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), dynamic mechanical analyzer (DMTA), X-ray diffraction (XRD), thermogravimetry (TG), mechanical test, chemical resistance and hardness test. The deconvolution analysis of FTIR of WPUs revealed that the hydrogen bond interaction in the WPUs increased with the increase of ISO content. Furthermore, the degree of microphase separation was increased with the increase of hydrogen bond interaction, which was confirmed by DSC, XRD and DMTA test. The results demonstrated that the addition of ISO can increase the hydrogen bond interaction between the hard and hard segments in WPUs, which improved the mechanical properties of the WPU films. The highest tensile strength was obtained of 59.67 MPa when the content of isosorbide was 10.35 wt%. Moreover, the ISO was helpful to improve the chemical resistance and hardness of the coatings by the comprehensive performance analysis.

Transcription Analysis of Recombinant Trichoderma reesei HJ-48 to Compare the Molecular Basis for Fermentation of Glucose and Xylose

( Jun Huang ),( Mei Lin ),( Shijie Liang ),( Qiurong Qin ),( Siming Liao ),( Bo Lu ),( Qingyan Wang ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.10

Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using next-generation sequencing technology. During xylose fermentation, numerous genes related to central metabolic pathways, including xylose reductase (XR) and xylitol dehydrogenase (XDH), were expressed at higher levels in T. reesei HJ-48. Notably, growth on xylose did not fully repress the genes encoding enzymes of the tricarboxylic acid and respiratory pathways. In addition, increased expression of several sugar transporters was observed during xylose fermentation. This study provides a valuable dataset for further investigation of xylose fermentation and provides a deeper insight into the various genes involved in this process.

Piezoelectric 6-dimensional accelerometer cross coupling compensation algorithm based on two-stage calibration

Jun Liu,Dengzhuo Zhang,Min Liu,Tongbao Zhu,Lan Qin,Jingcheng Liu 국제구조공학회 2023 Smart Structures and Systems, An International Jou Vol.32 No.2

In order to improve the measurement accuracy of the 6-dimensional accelerometer, the cross coupling compensation method of the accelerometer needs to be studied. In this paper, the non-linear error caused by cross coupling of piezoelectric sixdimensional accelerometer is compensated online. The cross coupling filter is obtained by analyzing the cross coupling principle of a piezoelectric six-dimensional accelerometer. Linear and non-linear fitting methods are designed. A two-level calibration hybrid compensation algorithm is proposed. An experimental prototype of a piezoelectric six-dimensional accelerometer is fabricated. Calibration and test experiments of accelerometer were carried out. The measured results show that the average nonlinearity of the proposed algorithm is 2.2628% lower than that of the least square method, the solution time is 0.019382 seconds, and the proposed algorithm can realize the real-time measurement in six dimensions while improving the measurement accuracy. The proposed algorithm combines real-time and high precision. The research results provide theoretical and technical support for the calibration method and online compensation technology of the 6-dimensional accelerometer.

Orthogonal 방법을 통한 Poly(AM-DMDAAC)/MMT 고흡수성나노복합체 제조 연구

Jun Dong Yuan,Ming Zhou,Shuang Qiao Yang,Yong Guo Zhou,Nan Qin,Song Tao He,Dong Lai,Zhong Qiang Xie 한국고분자학회 2014 폴리머 Vol.38 No.1

A novel poly(AM-DMDAAC)/MMT superabsorbent nanocomposites are prepared by radical polymerizationusing ammonium persulfate (APS) and anhydrous sodium sulfite as a free radical initiator and N,N-methylene bisacrylamide(MBA) as a crosslinker. In this paper, an optimization study on the synthesis of superabsorbent nanocompositesis carried out. Orthogonal array experiment indicates that the optimized conditions is acrylamide (AM) content 23 wt%,diallyl dimethyl ammonium chloride (DMDAAAC) content 6 wt%, montmorillonite (MMT) content 4 wt%, initiatorcontent 0.2 wt% and crosslinker content 0.02 wt%. Under the optimization syntheses conditions concluded, the maximumwater absorbency in distilled water is 659.53 g·g-1 and in 2 wt% sodium chloride solution is 116.25 g·g-1. Compared withthe range values of different factors (Rj), the order of significance factors in distilled water is C (MMT) > B (DMDAAC)> A (AM) > D (crosslinker) > E (initiator). MMT is intercalated during polymerization reaction and a nanocompositestructure is formed as shown by TEM analysis and XRD analysis.

review on the research of mechanical problems with different moduli in tension and compression

Jun-yi Sun,Hai-qiao Zhu,Shi-hong Qin,Da-lin Yang,Xiao-ting He 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.9

Most materials exhibit different tensile and compressive strains given the same stress applied in tension or compression. These materials are known as bimodular materials. An important model of bimodular materials is the criterion of positive-negative signs of principal stress proposed by Ambartsumyan. This model is mainly applicable to isotropic materials and deals with the principal stress state in a point. However, due to the inherent complexity of the constitutive relation, FEM based on iterative strategy and analytical methods based on a simplified mechanical model are required. In this paper, we review the basic assumptions of this model and its development, several innovative computational methods, and some important engineering applications. We also discuss the sequent key problems in this field.

Reports ; Adrenergic receptor β2 activation by stress promotes breast cancer progression through macrophages M2 polarization in tumor microenvironment

( Jun-fang Qin ),( Feng-jiao Jin ),( Ning Li ),( Hai-tao Guan ),( Lan Lan ),( Hong Ni ),( Yue Wang ) 생화학분자생물학회 2015 BMB Reports Vol.48 No.5

Stress and its related hormones epinephrine (E) and norepinephrine (NE) play a crucial role in tumor progression. Macrophages in the tumor microenvironment (TME) polarized to M2 is also a vital pathway for tumor deterioration. Here, we explore the underlying role of macrophages in the effect of stress and E promoting breast cancer growth. It was found that the weight and volume of tumor in tumor bearing mice were increased, and dramatically accompanied with the rising E level after chronic stress using social isolation. What is most noteworthy, the number of M2 macrophages inside tumor was up-regulated with it. The effects of E treatment appear to be directly related to the change of M2 phenotype is reproduced in vitro. Moreover, E receptor ADRβ2 involved in E promoting M2 polarization was comprehended simultaneously. Our results imply psychological stress is influential on specific immune system, more essential for the comprehensive treatment against tumors. [BMB Reports 2015; 48(5): 295-300]