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      • KCI등재

        Catalytic pyrolysis of corncob with Ni/CaO catalysts for hydrogen-rich gas: Synthesis modes and catalyst/biomass ratios

        Hongyu Liu,Yuting Tang,Xiaoqian Ma,Wenchang Yue 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.123 No.-

        With the depletion of traditional fossil fuels, biomass has evolved as a new environmentally friendly andsustainable alternative energy source. Ni/CaO catalysts are promising for CO2 capture and biomass catalyticuse. In this study, we examined four different synthesis modes for Ni/CaO catalysts: impregnationmethod, sol–gel method (citric acid complexation), sol–gel method with propionic acid modification andsedimentation method, and used the catalysts in the pyrolysis of corncob to produce hydrogen-rich gas at600 C. According to the results of the fixed-bed experiments, adding the propionic acid modification tothe citric acid complexation boosted hydrogen generation by 24.93 vol.%. The global behavior of the catalystssynthesized by the different methods follows the order sol-Ni1/Ca7-P > sol-Ni1/Ca7 > sed-Ni1/Ca7imp-Ni1/Ca7-A. At a catalyst/biomass ratio of 2:1, the maximum H2 concentration of 84.45 ± 1.02vol.% and H2 production of 26.84 ± 1.26 mmol/gbiomass were attained, as well as CO2 concentrations of just2.08 ± 0.03 vol.%. The H2 production was about 20 times higher than without the addition of the catalyst. Optimizing the synthesis modes and catalyst/biomass ratio produced high-quality hydrogen-rich gaswith a high H2 conversion of 88.95 ± 2.20 % and the energy efficiency of 52.73 %.

      • SCISCIESCOPUS
      • SCISCIESCOPUS

        Differently-charged graphene-based multilayer films by a layer-by-layer approach for oxygen gas barrier application

        Liu, Hongyu,Wu, Jianping,Liu, Cuiyun,Pan, Bingli,Kim, Nam Hoon,Lee, Joong Hee Elsevier 2018 Composites Part B, Engineering Vol.155 No.-

        <P><B>Abstract</B></P> <P>Polyelectrolyte-decorated graphene with positive and negative charges was prepared using graphene oxide as a precursor along with polyallylamine hydrochloride and poly (styrenesulfonate sodium) as surface modifiers, respectively. Driven by an electrostatic interaction, the as-obtained polyelectrolyte-decorated graphene sheets were self-assembled via a layer-by-layer method, producing graphene based composite multilayers. Field emission scanning electron microscopy (FE-SEM) images showed that the surface of composite film exhibited a mass of characteristic wrinkles of graphene. Interestingly, the cross-sectional FE-SEM of the film exhibited an unstratified structure, indicating a good dispersion of graphene sheets in the composite film. The results of oxygen gas permeability testing demonstrated that the resulting composite films exhibited excellent oxygen gas barrier properties. The oxygen gas transmission rate (OTR) of the composite film with 24 bilayers was as low as 1.2 cm<SUP>3</SUP> m<SUP>-2</SUP> d<SUP>-1</SUP>∙atm<SUP>−1</SUP>, exhibiting potential for use in packaging applications.</P>

      • Effect of kisspeptin on the proliferation and apoptosis of bovine granulosa cells

        Hongyu Liu,Myeong-Don Joo,Ji-Yoon Hwang,Ayman Mesalam,Jun Wang,Wenfa Lu,Il-Keun Kong 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

        Previous studies have shown that kisspeptin (Kp-10) is expressed in mammalian ovaries; however, the expression and role of Kp-10 in bovine ovarian granulosa cells are still unclear. In this study, we assessed the expression of Kp-10 and its effects on the proliferation and apoptosis of bovine granulosa cells. Immunohistochemical analysis showed that Kp-10 was expressed in the cytoplasm of bovine ovarian granulosa cells. Moreover, MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2- H-tetrazolium bromide) assays showed that 100 nM Kp-10 significantly inhibited the viability of granulosa cells (P<0.01). Flow cytometry analysis showed that Kp-10 could significantly increase accumulation of cells in the G1 phase, decrease accumulation of cells in the S phase, and promote apoptosis in bovine granulosa cells (P<0.05). Additionally, Kp-10 decreased the mRNA levels of Bcl-2, an anti-apoptotic gene; increased the mRNA levels of caspase-3, a pro-apoptotic gene; and increased the mRNA levels of Fas and Fasl, two membrane surface molecule genes (P<0.05). Thus, our findings demonstrated for the first time that Kp-10 inhibited proliferation and promoted apoptosis in bovine ovarian granulosa cells. These findings provide insights into our understanding of the role of Kp-10 in mediating the proliferation of bovine granulosa cells.

      • KCI등재
      • KCI등재

        A new sexually dimorphic mantidfly species of Allomantispa Liu et al., 2015 from China (Neuroptera: Mantispidae)

        Li Hongyu,Wu Chao,Ohl Michael,Liu Xingyue 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

        Allomantispa Liu et al., 2015 is the only extant genus of the mantidfly subfamily Drepanicinae from Eurasia, being endemic to the Oriental region. It currently consists of only two species from southeastern Tibet and northern Myanmar, respectively. Here we describe a new species of this rarely observed mantidfly genus, namely Allomantispa coniprocessa sp. nov. from Yunnan, China. Interestingly, the new species displays distinctive sexual dimorphic characters on the pronotum. The molecular identification reveals obvious genetic divergence between the allopatric individuals of the new species. Their morphological variations are illustrated and compared. In addition, some characters with taxonomic or phylogenetic significance present on the pronotum, the abdomen and the genitalia in Allomantispa are also discussed. Our finding updates the morphological and distributional information on Allomantispa and implies a more diverse fauna of this genus in the Oriental region than pre viously assumed.

      • KCI등재

        Comparative transcriptomic and physiological analyses of contrasting hybrid cultivars ND476 and ZX978 identify important differentially expressed genes and pathways regulating drought stress tolerance in maize

        Guo Liu,Tinashe Zenda,Songtao Liu,Xuan Wang,Hongyu Jin,Anyi Dong,Yatong Yang,Huijun Duan 한국유전학회 2020 Genes & Genomics Vol.42 No.8

        Background Drought is the major abiotic stress factor that negatively influences growth and yield in cereal grain crops such as maize (Zea mays L.). A multitude of genes and pathways tightly modulate plant growth, development and responses to environmental stresses including drought. Therefore, crop breeding efforts for enhanced drought resistance require improved knowledge of plant drought responses. Objective Here, we sought to elucidate the molecular and physiological mechanisms underpinning maize drought stress tolerance. Methods We therefore applied a 12-day water-deficit stress treatment to maize plants of two contrasting (drought tolerant ND476 and drought sensitive ZX978) hybrid cultivars at the late vegetative (V12) growth stage and performed a large-scale RNA sequencing (RNA-seq) transcriptome analysis of the leaf tissues. Results A comparative analysis of the two genotypes leaf transcriptomes and physiological parameters revealed the key differentially expressed genes (DEGs) and metabolic pathways that respond to drought in a genotype-specific manner. A total of 3114 DEGs were identified, with 21 DEGs being specifically expressed in tolerant genotype ND476 in response to drought stress. Of these, genes involved in secondary metabolites biosynthesis, transcription factor regulation, detoxification and stress defense were highly expressed in ND476. Physiological analysis results substantiated our RNA-seq data, with ND476 exhibiting better cell water retention, higher soluble protein content and guaiacol peroxidase activity, along with low lipid peroxidation extent than the sensitive cultivar ZX978 under drought conditions. Conclusion Our findings enrich the maize genetic resources and enhance our further understanding of the molecular mechanisms regulating drought stress tolerance in maize. Additionally, the DEGs screened in this study may provide a foundational basis for our future targeted cloning studies.

      • KCI등재

        Excessive copper in feed not merely undermines animal health but affects food safety

        Zicheng Ma,Yan Li,Zifeng Han,Zhaohu Liu,Hongyu Wang,Fanliang Meng,Sidang Liu,Dawei Chen,Mengda Liu 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.3

        Background: Blackened intestines in slaughtered pigs have been commonly observed in China in recent years. However, no cause has been reported. Objectives: We attempted to determine whether the blackening of the pig intestine was related to an excess of copper (Cu) in their feed. Methods: In this study, we observed and collected porcine intestines in small- and large-scale pig slaughterhouses in Shandong province from May to October 2018. Twelve types of metal ions were detected in the black intestinal samples. Results: The Cu level in the intestine samples was mostly higher than the Chinese national limit for food. Further study showed that Cu supplementation in most commercial porcine feed also exceeded the national standard. An animal model (mouse) that could mimic the intestinal blackening in pigs was established. Compared to control mice, Cu accumulated in the liver and intestines of mice fed an excessive Cu level, confirming the excessive Cu in the feed may be considered the major cause of blackened porcine intestines. Microscopic examination revealed that black intestines had many particles containing Cu in the lamina propria of the intestinal mucosa, and the intestinal mucosal epithelial cells showed degeneration and necrosis. Conclusions: In conclusion, overuse of Cu in animal feed can lead to animal poisoning and Cu accumulation in animal products. Such overuse not only harms the health of livestock but can also affect public health.

      • KCI등재

        Epidemiological Investigation of Porcine Pseudorabies Virus and Its Coinfection Rate in Shandong Province in China From 2015 to 2018

        Zicheng Ma,Zifeng Han,Zhaohu Liu,Fanliang Meng,Hongyu Wang,Longlong Cao,Yan Li,Qiulin Jiao,Sidang Liu,Mengda Liu 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

        Background: Pseudorabies, also known as Aujeszky's disease, is caused by the pseudorabies virus (PRV) and has been recognized as a critical disease affecting the pig industry and a wide range of animals around the world, resulting in great economic losses each year. Shandong province, one of the most vital food animal-breeding regions in China, has a very dense pig population, within which pseudorabies infections were detected in recent years. The data, however, on PRV epidemiology and coinfection rates of PRV with other major swine diseases is sparse. Objectives: This study aimed to investigate the PRV epidemiology in Shandong and analyze the current control measures. Methods: In this study, a total number of 16,457 serum samples and 1,638 tissue samples, which were collected from 362 intensive pig farms (≥ 300 sows/farm) covered all cities in Shandong, were tested by performing enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Results: Overall, 52.7% and 91.5% of the serum samples were positive for PRV-gE and -gB, respectively, based on ELISA results. In addition, 15.7% of the tissue samples were PCR positive for PRV. The coinfection rates of PRV with porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus, and classical swine fever virus were measured; coinfection with PCV2 was 35.0%, higher than those of the other two viruses. Macroscopic and microscopic lesions were observed in various tissues during histopathological examination. Conclusions: The results demonstrate the PRV prevalence and its coinfection rates in Shandong province and indicate that pseudorabies is endemic in pig farms in this region. This study provides epidemiological data that can be useful in the prevention and control of pseudorabies in Shandong, China.

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