Synthesis of carbamates from amines and N-tosylhydrazones under atmospheric pressure of carbon dioxide without an external base

Hong, Ji Young,Seo, Ue Ryung,Chung, Young Keun The Royal Society of Chemistry 2016 ORGANIC CHEMISTRY FRONTIERS Vol.3 No.6

<▼1><P>A synthetic method to carbamates from amines and <I>N</I>-tosylhydrazones in the presence of 1 atm of carbon dioxide was developed.</P></▼1><▼2><P>The methodology for the synthesis of carbamates <I>via</I> the base-promoted coupling of carbon dioxide, amines, and <I>N</I>-tosylhydrazones using potassium carbonate as the base and the pressure of 4 MPa of carbon dioxide has been published by Jiang <I>et al.</I> (2015). We demonstrated that this method provides the desired carbamates under just 1 atm of carbon dioxide without the addition of a base promoter. Thus, carbamates were successfully synthesized under mild reaction conditions in moderate to high yields.</P></▼2>

P128 : Mohs micrographic surgery for extramammary Paget`s disease: a study of 60 Asian patients

( Jimyung Seo ),( Hee Su Kim ),( Ji Hee Kim ),( Hong Sun Jang ),( Byung Ho Oh ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Extramammary Paget disease (EMPD) is an uncommon intraepithelial tumor characterized by ill-defined margins and high recurrence rates after wide local excision. Objectives: To evaluate the efficacy of MMS for EMPD and establish surgical treatment guidelines for EMPD in Asian patients. Methods: The results of 60 patients with EMPD treated by MMS from 2000 to 2013 have been analyzed retrospectively. Results: There were 54 male and 60 female patients and most were treated by slow-MMS using permanent paraffin-embedded sections. Among 60 patients, 58 had genital lesions and 2 had axillary lesions. The average follow-up period was 61.5 months (14 -140 months). The recurrence rate of EMPD after MMS was 3.3% (n=2) and the mean time to recurrence was 11 months. Among the recurred patients, one had been previously treated by topical 5% imiquimod and the other had undergone prior wide local excision. Estimated 5-year tumor-free rate using Kaplan-Meier graph was 96.3% in all patients for MMS. None of the primarily occurring cases recurred, indicating 0% (n=50) recurrence rate. However, among patients who underwent MMS after initial recurrence, the recurrence rate was significantly higher at 20% (n=10). Conclusion: Our results demonstrate that the recurrence rate of EMPD is low (3.3%) after MMS. MMS is an effective surgical method with low recurrence rate, and can be suggested as the first-line treatment for non-metastatic primary EMPD patients and also for patients with recurred disease.

P129 : The efficacy of Mohs micrographic surgery for the treatment of dermatofibrosarcoma protuberans: a report of 50 Asian patients

( Jimyung Seo ),( Hee Su Kim ),( Ji Hee Kim ),( Hong Sun Jang ),( Byung Ho Oh ),( Mi Ryung Roh ),( Kee Yang Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Background: Dermatofibrosarcoma protuberans (DFSP) is a rare, invasive dermal mesenchymal tumor known to have high risk of local recurrence. Recently, there have been preliminary reports of DFSP successfully treated by MMS with favorable cure rates. Objectives: To review data of 50 DFSP patients treated by MMS at Severance Hospital and summarize the accumulated experience Methods: The results of 50 DFSP patients treated by MMS from 2000 to 2013 were analyzed retrospectively. Results: There were 23 male and 27 female patients and most were treated by slow-MMS using permanent paraffin-embedded sections. The trunk (60%), followed by the extremities, and head and neck, were the most common sites for DFSP. Two stages of MMS were sufficient to achieve clearance in the majority of cases and all patients treated had clear margins at final stages. Local flaps including complex flaps were the most common reconstruction methods to repair the surgical defects (95%). The average follow-up period was 52.5 months (13 -85 months). Two out of 50 patients developed local recurrence and underwent a second MMS. However, two recurred patients had been treated by wide local excision before being referred to our institution for the MMS. Conclusion: Our results demonstrate that the recurrence rate of DFSP is low (4%) after MMS. Furthermore, there was no recurrence in the cases of primary DFSP lesions during the follow-up period. MMS can be suggested as a safe and effective treatment method with low recurrence rate.

케이신 포스포펩티드/키토올리고당 나노 복합체 형성과 특성 연구

백윤서 ( Yun-seo Baek ),하호경 ( Ho-kyung Ha ),이지홍 ( Ji-hong Lee ),이미령 ( Mee-ryung Lee ),이원재 ( Won-jae Lee ) 한국유가공기술과학회 2018 한국유가공기술과학회지 Vol.36 No.3

본 연구에서는 CPP와 CSO를 사용하여 약 187∼282 nm 크기의 균일한 구형의 나노 복합체를 성공적으로 제조하였다. 나노 복합체는 제조 공정 요인인 CPP 농도와 pH 조절을 통해 입자 크기, 다분산지수, 그리고 표면 전하와 같은 나노 복합체의 물리화학적 특성을 조절할 수 있음을 확인하였다. 또한 CPP/CSO 나노 복합체의 분말화 공정을 통해 분말화 된 나노 복합체의 식품 적용성을 증진시킬 수 있음을 알 수 있었으며, food-grade 물질인 CPP와 CSO를 사용하여 제조한 나노 복합체는 향후 잠재적인 칼슘 전달체로써 식품 산업에 이용될 수 있을 것으로 기대된다. The objectives of this study were to manufacture casein phosphopeptide (CPP)/chitosan oligosaccharide (CSO) nanocomplexes and to investigate the impacts of manufacturing variables, such as CPP concentration and pH, on their morphological and physicochemical characteristics. Transmission electron microscopy (TEM) and particle size analysis were used to assess the morphological and physicochemical properties of the CPP/CSO nanocomplexes, respectively. Based on the images obtained by TEM, the spherical shapes of the CPP/CSO nanocomplexes ranged from 50 to 150 nm. As the concentration of CPP was increased and the pH was decreased, the average particle size of the nanocomplexes significantly (p<0.05) increased. The CPP/CSO nanocomplexes had a highly uniform distribution with a polydispersity index value of less than 0.3. In addition, they had a negative surface charge with a zeta-potential value between ―17 and ―26 mV. The CPP/CSO nanocomplexes showed good stability during the freeze-drying process. In conclusion, CPP/CSO nanocomplexes were successfully manufactured, and the CPP concentration and pH were found to be key factors that affected their morphological and physicochemical properties.

Therapeutic application of metallic nanoparticles combined with particle-induced x-ray emission effect

Kim, Jong-Ki,Seo, Seung-Jun,Kim, Ki-Hong,Kim, Tae-Jeong,Chung, Myung-Hwan,Kim, Kye-Ryung,Yang, Tae-Keun IOP Pub 2010 Nanotechnology Vol.21 No.42

<P>Metallic nanoparticles (MNP) are able to release localized x-rays when activated with a high energy proton beam by the particle-induced x-ray emission (PIXE) effect. The exploitation of this phenomenon in the therapeutic irradiation of tumors has been investigated. PIXE-based x-ray emission directed at CT26 tumor cells <I>in vitro</I>, when administered with either gold (average diameter 2 and 13 nm) or iron (average diameter 14 nm) nanoparticles (GNP or SNP), increased with MNP solution concentration over the range of 0.1–2  mg ml<SUP> − 1</SUP>. With irradiation by a 45 MeV proton therapy (PT) beam, higher concentrations had a decreased cell survival fraction. An <I>in vivo</I> study in CT26 mouse tumor models with tumor regression assay demonstrated significant tumor dose enhancement, thought to be a result of the PIXE effect when compared to conventional PT without MNP (radiation-only group) using a 45 MeV proton beam (<I>p</I> < 0.02). Those receiving GNP or SNP injection doses of 300 mg kg<SUP> − 1</SUP> body weight before proton beam therapy demonstrated 90% or 75% tumor volume reduction (TVR) in 20 days post-PT while the radiation-only group showed only 18% TVR and re-growth of tumor volume after 20 days. Higher complete tumor regression (CTR) was observed in 14–24 days after a single treatment of PT with an average rate of 33–65% for those receiving MNP compared with 25% for the radiation-only group. A lower bound of therapeutic effective MNP concentration range, <I>in vivo</I>, was estimated as 30–79  µg g<SUP> − 1</SUP> tissue for both gold and iron nanoparticles. The tumor dose enhancement may compensate for an increase in entrance dose associated with conventional PT when treating large, solid tumors with a spread-out Bragg peak (SOBP) technique. The use of a combined high energy Bragg peak PT with PIXE generated by MNP, or PIXE alone, may result in new treatment options for infiltrative metastatic tumors and other diffuse inflammatory diseases. </P>

임신 중 당뇨의 조절은 어느 과에서 시행하는 것이 적절한가?: 내과와 산부인과에서의 비교

서용수 ( Yong Soo Seo ),신정환 ( Jung Hwan Shin ),이헌열 ( Hun Yul Lee ),박원일 ( Won Il Park ),김재령 ( Jae Ryung Kim ),김혜민 ( Hae Min Kim ),최은주 ( Eun Joo Choi ),김대운 ( Dae Woon Kim ),홍서유 ( Seo Yoo Hong ),박은주 ( Eun J 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.7

목적: 당뇨가 합병된 임신의 결과를 당뇨조절과에 따라 비교해 보고 임신 중 당뇨조절에 있어서 산부인과 의사와 내분비내과 의사의 역할을 고찰해 보고자 하였다 방법: 2004년 3월부터 2006년 12월 사이에 임신 중 당뇨병이 합병된 총 72명을 대상으로 후향적 연구를 시행하였다. 을지병원에서 임신 기간 중 산부인과에서 당뇨 조절을 받은 18명을 I군으로, 내분비 내과에서 당뇨 조절을 받은 54명을 II군으로 정의하여 임신의 결과를 비교 분석하였다. 다태임신, 염색체 이상, 당뇨 이외에 다른 내과적 합병증이 있는 경우는 연구 대상에서 제외하였다. 결과: 나이, 분만력, 신체비만지수, 임신 중 체중증가, 분만 주수 등의 인구통계학적 분포에서 두 군 간에 차이가 없었다. 100 gm 경구 당부하검사 결과, HbA1C 수치에서도 두 군 간에 차이가 없었다. 신생아 평균 체중은 I군 (2.89±0.21 kg)이 II군 (3.38±0.08 kg, P=0.040)에 비해 유의하게 낮은 결과를 보였다. 1분 아프가 점수도 ANCOVA 분석을 통한 신생아 체중을 보정 후에도 I군에서 II군에 비해 유의하게 낮은 결과를 보였다 (7.11±0.51 vs 8.11±0.19, P=0.028). 하지만, 5분 아프가 점수에서는 두 군 간에 차이가 없었다. 출생 체중이 3.8 kg 이상의 신생아 비율도 II군에서 유의하게 높은 소견을 보였다 [1/17 (5.6%) vs 37/54 (68.5%), P=0.030]. 신생아 저혈당증의 비율도 I군이 11.1%로 II군의 37.0%에 비해 유의하게 낮은 결과를 보였다 (P=0.043). 당뇨에 의한 다른 신생아와 임신부의 합병증의 비교에서는 두 군 간에 차이가 없었다. 결론: 내과적 관점에서, 임신 기간 중 당뇨가 합병된 임산부의 당뇨조절에서 산부인과에서의 조절도 부적절함이 없다. 또한 산부인과에서 혈당을 조절하는 경우 내과에서의 조절과 비교하여 더욱 경제적이고, 환자에게 번거로움이 적으며, 환자의 치료 동기와 순응도를 보다 높일 수 있을 것으로 생각된다. Objective: To compared obstetric outcomes of pregnancies complicated by diabetes according to the managing department and to consider role of obstetrician and endocrinologist Methods: A retrospective analysis of 72 pregnant women managed for diabetes during March 2004 to December 2006 was performed. Eighteen women had been managed by obstetricians (Group I) while 54 women had been managed by endocrinologists of internal medicine (Group II) during their pregnancies at Eulji Hospital. None of these patients had multiple pregnancies, abnormal karyotype, or other medical disorders other than diabetes. Pregnancy outcomes were compared between two groups. Results: No difference was found with regard to the age, parity, BMI (body mass index), weight gain during the pregnancy, and the gestational weeks of delivery. There was also no difference in the blood glucose levels after 100 gm OGTT (oral glucose tolerance test) and HbA1C levels in two groups. The neonatal birth weight of group I (2.89±0.21 kg) was significantly lower than that of group II (3.38±0.08 kg, P=0.040). The 1 min APGAR score, which was corrected by weight through ANCOVA test, was also significantly lower in group I (7.11±0.51 vs 8.11±0.19, P=0.028). But There was no difference in 5 min APGAR score. The incidence of birth weight over 3.8kg was significantly higher in group II [37/54 (68.5%) vs 1/17 (5.6%), P=0.030]. Neonatal hypoglycemia was also higher in group II (37.0% vs 11.1%, P=0.043). Other neonatal and maternal complications associated with diabetes were not significantly different between two groups. Conclusions: In medical point of view, obstetrician can effectively manage diabetes during pregnancy as good as internist. Besides, the management by obstetricians may be more economic, less annoying, and can induce more compliance and motivation of the patients.

개방각녹내장에서 야간혈압하강, 경동맥혈류저하, 뇌의 허혈성 변화가 녹내장 진행에 미치는 영향

서홍융,진상욱,노세현,Hong Ryung Seo,Sang Wook Jin,Sae Heun Rho 대한안과학회 2013 대한안과학회지 Vol.54 No.9

Purpose: To investigate the effect of nocturnal dip, carotid artery blood flow, and brain ischemic change on the progression of glaucomatous visual field defect in open-angle glaucoma (OAG) when IOP is less than the target pressure. Methods: We classified OAG patients (74 patients, 148 eyes) who maintained IOP less than the target pressure as normal tension glaucoma (NTG; 52 patients, 104 eyes) or primary OAG (POAG; 22 patients, 44 eyes). Additionally, we performed 24-hr ambulatory blood pressure monitoring (24-hr ABPM), carotid artery color Doppler U/S (CAD), brain MRI, and visual field (V/F) tests on the patients. Nocturnal dips less than 10% were classified as non-dippers, and dips greater than 10% as dippers. The relationships among nocturnal dip, carotid artery blood flow, brain ischemic change, and progression of glaucomatous V/F defect were examined. Results: In the case of dippers, glaucomatous V/F defects were aggravated, with a relative risk of approximately 1.74 (NTG) and 2.91 (POAG) times that of non-dippers. In NTG, decreased carotid artery blood flow and brain ischemic change furthered glaucomatous V/F defects, with a relative risk of approximately 2.40 and 2.54 times that of normal carotid artery blood flow and brain MRI findings, respectively. However, in POAG, decreased carotid artery blood flow and brain ischemic change were not influenced by the progression of glaucomatous V/F defects. Conclusions: In dippers, decreased carotid artery blood flow and brain ischemic change caused a progression of glaucomatous V/F defects in NTG and POAG patients. Thus, performing 24-hr ABPM, CAD, and brain MRI should be helpful for glaucoma patients with progression of glaucomatous V/F defects even when the IOP is less than the target pressure. In addition, this analysis provides useful information regarding glaucoma diagnosis and treatment.

P025 : Reconstruction of large wounds using a combination of negative pressure wound therapy (NPWT) and punch skin grafts

( Howard Chu ),( Ji Myung Seo ),( Ji Hee Kim ),( Hong Sun Jang ),( Dan Bi Lee ),( Byung Ho Oh ),( Mi Ryung Roh ),( Kyoung Ae Nam ),( Kee Yang Chung ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2

Objectives: The objective of this study was to evaluate the efficacy of a combination of NPWT and punch skin grafts in the reconstruction of large wounds. Methods: This study is a retrospective review of 9 patients, who were treated using the combination treatment during the period of Oct. 2013 and Apr. 2014. Results: Of the 9 cases, 8 were the result of melanoma wide excision and 1 was a nonhealing wound after a fracturesurgery. Mean patient age was 58 years (range, 32~82). Mean period of NPWT was 22.5 days before skin graft and 13.6 days after skin graft. Among the 9 cases, sole was the most common location of the wound (4 cases) followed by heel (2 cases), right 5th toe (1 case), left popliteal fossa (1 case) and right shin (1 case). NPWT induced rapid growth of granulation tissue and seemed to facilitate graft take and survival. Conclusion: A combination treatment of NPWT and punch skin graft can be a viable option to treat large wounds. Theresulting scar is much smaller than the size of the wound due to contraction and also cosmetically and functionally superior to the wound treated by other methods.

야간혈압하강이 녹내장의 진행에 미치는 영향

서홍융,류원열,노세현,Hong Ryung Seo,Won Yeol Ryu,Sae Heun Rho 대한안과학회 2010 대한안과학회지 Vol.51 No.11

Purpose: To investigate the effect of nocturnal dip influence on the progression of glaucomatous visual field defect. Methods: We performed 24hr ABPM and V/F tests on patients diagnosed with NTG (140 patients, 280 eyes) and POAG (84 patients, 168 eyes). Nocturnal dips below 10% were classified as non-dippers, and those above 10% were noted as dippers. The correlations among nocturnal dip, progression of glaucomatous visual field defect, and hypertension treatment were examined. Results: In NTG, dippers in both systolic and diastolic blood pressure furthered glaucomatous visual field defects, with a relative risk of approximately three times that of non-dippers. Hypertension treatment was not influenced by the progression of glaucomatous visual field defect but was influenced by dips in the systolic and diastolic pressures in NTG and in diastolic pressure in POAG. Nocturnal dips were more frequent in the group with progression of the visual field compared to those in the group with non-progression of the visual field in NTG. Conclusions: Dipper caused a progression of glaucomatous visual field defects in NTG and was influenced by hypertension treatment in NTG and POAG. Performing 24hr ABPM should be helpful for glaucoma patients with progression of a glaucomatous visual field defect even when the IOP is less than the target pressure. In addition, hypertension treatment should be considered an important factor in the treatment of glaucoma. J Korean Ophthalmol Soc 2010;51(11):1471-1478

한국인의 스펙트럼영역빛간섭단층촬영 변수의 나이에 따른 차이

서지영,서홍융,노세현,Ji Young Suh,Hong Ryung Seo,Sae Heun Rho 대한안과학회 2013 대한안과학회지 Vol.54 No.2

Purpose: We evaluated the thickness of RNFL and optic nerve head parameters with age in normal eyes using Spectral-Domain Optical Coherence Tomography (SD-OCT). Methods: A total of 128 normal Korean volunteers in different age groups were recruited (age range, 20-70 years). Results: A significant negative correlation in average RNFL thickness with increasing age was found. The inferior areas (130.31 ± 3.33 μm) were significantly thicker than other areas (superior area 119.05 ± 2.12 μm, nasal area 71.80 ± 0.57 μm, temporal area 77.72 ± 0.16 μm). The average C/D ratios (mean 0.38 ± 0.14) and vertical C/D ratios (mean 0.35 ± 0.11) both showed significant increases with age, and the vertical C/D ratio correlation coefficient was higher (average C/D ratio r = 0.249, vertical C/D ratio r = 0.537). However, rim area, disc area, and cup volume were not correlated with age. Conclusions: From these findings, we conclude that, in normal Koreans, the mean RNFL thickness decreases and the C/D ratio increases with age, with the increase in the vertical C/D ratio being greater.