김치에서 분리한 Lactobacillus sp. JC-7과 Lactobacillus acidophilus 88간의 Electrofusion 최적조건 설정

조영배,최현정,백형석,전홍기 부산대학교 유전공학연구소 1997 분자생물학 연구보 Vol.13 No.-

김치 발효 숙성기간을 연장하고 신선도를 오랫동안 유지 할 수 있는 김치발효 starter의 개발을 목적으로 최적숙성기에 있는 김치로부터 bacteriocin생성능이 없는 유산균을 분리하여 동정하였으며, bacteriocin생성능이 우수한 L.acidophilus 88을 융합시키기 위해 electrofusion에 대한 최적 조건을 검토하였다. 분리균주는 Lactobacillus속으로 동정되어 Lactobacillus sp. JC-7이라 명명하였다. Electrofusion에 의해 생성된 융합주를 식별하기 위해 streptomycin(2.5㎎/ml)에 내성을 나타내는 Lactobacillus sp. JC-7변이주와 kanamycin(600㎍/ml)에 내성을 나타내는 L.acidophilus 88변이주를 분리하였다. Electrofusion을 100V/㎝, 120msec(72ohms,1670 capacitance) 에서 수행했을 때 융합효율이 가장 양호하였으며 전기장의 세기와 시간이 중가할수록 융합 효율이 현저히 감소하였다. 2가 양이온은 농도가 중가할수록 대체적으로 융합효율을 감소시키는 경향을 나타내었으나 1mM MGCl_2에서는 대조군에 비해 융합효율이 약간 증가하였다. PEG매개에 의한 융합법의 융합효율을 비교한 결과, 융합효율은 chemical fusion<electrofusion<electrofusion+20% PEG순을 나타내었다. Evaluation of Optimum Conditions for the Electrofusion between Lactobacillus sp. JC-7 Isolated from Kimchi and Lactobacillus acidophilus 88. Young-Bae Jo, Hyun-Jung Choi, Hyung-Suk Baik and Hong-Ki Jun*. Department of Microbiology, Pusan National University, Pusan 609-735,Korea-A lactic acid bacterium was isolated from kimchi. The isolated strain was identified as the genus Lactobacillus thrugh its morphological characteristics and named as Lactobacillus sp. JC-7. The optimum conditions for the electrofusion between streptomycin(2.5㎎/ml)resistant mutant of Lactobacillus acidophilus 88 and kanamycin(600㎍/ml) resistant mutant Lactobacillus sp. JC-7 were evaluated. The highest number of fusants were obtained at a capacitance value of 120msec(1670㎌), a field strength of 100V/㎝,and a pulse controller setting of 72Ω. The potimum pH of elecroporation buffer was 7.5 and the concentration of divalent cation was 1mM MG^2+. Electrofusants were efficiently obtained by addition 20% polyethylene glycol to electroporation buffer. The yield of fusion was better than that of using polyethylene glycol mediated chemical induction.

Lactobacillus casei와 Lactobacillus delbrueckii간의 Protoplast 융합에 관한 연구

전홍기,김미경,백형석 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.1

유산균주의 균주개량방법의 일환으로 protoplast fusion 방법을 사용하여 lincomycin에 내성을 나타내는 Lactobacillus casei KCTC 1121과 rifampicin에 내성을 나타내는 Lactobacillus delbrueckii JK-414의 protoplast 형성과 재생, 융합에 대한 조건 및 융합주의 생리학적 성질 등을 검토하였다. Lactobacillus casei와 Lactobacillus delbrueckii JK-414는 삼투압 안정제로 sucrose가 함유된 protoplast forming buffer에서 5㎍/㎖의 mutanolysin으로 42℃, 15분간 처리했을 때 protoplast 형성율이 높게 나타났다. L. casei와 L. delbrueckii는 대수 증식기 중반에서 protoplast가 가장 잘 형성되었으며, MRS 배지에 삼투압 안정제로 sucrose 10%, MgCl_2 6 mM, CaCl_2 6 mM, gelatin 2.5%를 첨가하여 만든 재생배지에서 재생효율이 가장 좋았다. 한편, L. casei와 L. delbrueckii 사이의 protoplast 융합은 40%의 PEG 4,000을 처리하였을 때 가장 양호하였으며, 그 융합 효율은 3.2×10^-4이었다. L. casei가 L. delbrueckii보다 높은 산 생성능을 보였으며 융합주 중 특히 F23, F35의 산 생성능이 우수하였다. 융합주 중 F23, F24의 protease 활성이 모균주의 protense 활성보다 높았으며, 이들 융합주의 DNA 함량은 모균주의 2배였다. Protoplast fusion between lincomycin resistant Lactobacillus cαsei KCTC 1121 and rifampicin resistant Lactobacillus delbrueckii JK-414 was attempted to obtain the improved strains. Protoplasts of L. casei and L. delbrueckii were produced by mutanolysin digestion at 42℃ for 15 min. L. casei cells were converted to protoplasts by treating with 5㎍/㎖ of mutanolysin in 20 mM HEPES buffer (pH 7.0) containing 0.75 M sucrose at the middle logarithmic growth phase. In case of L. delbrueckii 1.0 M sucrose was used osmotic stabilizer. Regeneration of protoplast in both strains was efficiently accomplished on the regeneration medium containing 10% sucrose, 6 mM MgCl_2, 6 mM CaCl_2, and 2.5% gelatin. Protoplast fusion between L. casei and L. delbrueckii was carried out in the presence of 40% of PEG 4,000. The frequency of protoplast fusion was found to be about 3.2×10_-4. Acid production of L. casei was better than that of L. delbrueckii. Among fusants, F23 and F35 exhibited excellent lactic acid production. F23 and F24 exhibited the improved proteolysis compared to that of the parent strains and they had twice as much as DNA content of the parents.

표재성 피부 지방종성 모반 2례

홍진표,송준영,김병천,이규석 啓明大學校 醫科大學 1986 계명의대학술지 Vol.5 No.2

Nevus lipomatosus cutaneus superficialis is very rare malformation consisting of multiple or solitary papules usually occuring on the lower trunk or glutesl regions. Microscopically it reveals groups of ectopic fat cell nests within the dermis. The authors observed two cases of typical nevus lipomatosus cutanes superficaialis. The first case was a 30-year-old female who has had asymptomatic, soft, skin colored and huge confluent tomor masses on the left buttock for 10 years; the second case was a 21-year-old male who has had multipel, soft, skin colored papules on the right buttock for 10 years. The author, made diagnosis of nevus lipomatosus cutaneus superficialis from characteristic clinical and histopathological findings. Related literature was briefly reviewed for the discussion.

김치 분리균인 Bacillus sp. JK-43이 생산하는 Cyclodextrin Glucanotransferase의 생산 및 특성

전홍기,배경미,김영희,백형석 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-

김치 시료로부터 자동산화되지 않으며 열 및 중성 pH에서 안정한 AA 유도체인 AA-2G를 생산할 수 있는 당전이활성을 가진 CGTase 생산균주를 분리하였고, 분리균주의 형태학적, 배양학적, 생리학적 성질 및 16s-rDNA sequences를 조사한 결과 그람 양성의 간균으로 호기성이며 내생포자를 형성하는 전형적인 중온성 Bacillus sp. JK-43으로 동정되었다. Bacillus sp. JK-43의 CGTase는 AA-2G 뿐만 아니라 AA-6G로 추정되는 물질을 함께 생산하였으며, 효소 최적생산조건은 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4 그리고 0.02% MgSO_4·7H_2O가 함유된 배지에서 pH 7.0, 37℃에서 26시간 동안 진탕배양하였을 때였다. 각종 당공여체에 따른 Bacillus sp. JK-43의 AA-2G 생산성을 조사한 결과 β-CD에서 가장 높은 AA-2G 생산성을 보였으며, 식혜제조폐액인 엿기름 및 밥당화액에서도 비교적 높은 AA-2G 생산성을 보였다. 또한 여러 가지 당수용체에 대한 JK-43의 CGTase의 당전이 반응을 검토한 결과 sucrose, mannitol 및 inositol에서 높은 당전이 수율인 70∼90%를 나타내었다. A bacterial strain, designated as JK-43, producing extracellular cyclodextrin hlucanotransferase (CGTase)[EC 2.4.1.19] was isolated from kimchi. The CGTase from isolated strain JK-43 showed the transglucosylation activity from soluble starch to L-ascorbic acid (AA) compared to those obtained from other strains. A main product formed by this reaction was identified as 2-O-α-glucopyranosyl L-ascorbic acid (AA-2G) by testing its susceptibility to α-glucosidase hydrolysis, the HPLC prodiles, and through the elementary analysis. The β-CD, γ-CD, potato starch and corn starch were identified to be suitable glucosyl donor for transglucosylation reaction on AA by CGTase. Acceptor specificity on AA-2G production was examined by use of AA, Iso-AA and AA-2P. Transglucosylation was observed toward AA-2P as well as AA. The microorganism isolated from kimchi was identified as a strain of Bacillus sp. JK-43 based on the maximal CGTase production was observed in a medium containing 1.0% soluble starch, 1.0% yeast extract, 1.0% Na_2CO_3, 0.1% K_2HPO_4, and 0.02% MgSO_4·7H_2O with initial pH 7.0. The strain was cultured at 37℃ for 26 hrs with reciprocal shaking.

부여-함평분지에 분포하는 백악기 퇴적암에 대한 고지자기연구

홍준표,석동우 한양대학교 이학기술연구소 2004 이학기술연구지 Vol.7 No.

본 연구에서는 부여분지와 함평분지에 분포하는 백악기 퇴적암에 대한 고지자기 연구를 수행하였다. 한반도 남서부에 분포하는 백악기 퇴적분지인 부여, 함평분지에 생성된 백악기 퇴적암에 대한 특성잔류자화 방향을 정립하기 위하여 충 279개의시료를 준비하였으며, 열소자와 교류 소자를 실시한 후 특성잔류자화 방향을 추출하였다. 부석분지의 경우 총 13개 site에서 120개의 정향시료를 채취하여 측정한 결과 경사보정 후의 방향으로 계산된 고지자기극은 Lat./Long.=69.9˚N/193.7˚E (dp=11.5˚, dm=15.1˚)으로 한반도 중기 백악기의 극과 유사하다. 또한 함평분지의 경우 총 20개 site에서 159개의 정향시료를 채취하여 측정한 결과 경사보정후의 방향으로 계산된 고지자기극의 위치는 Lat/Long.=63.7˚N/205.2˚E(dp=8.8˚, dm=12.4˚)로 한반도 중기 백악기의 고지자기극의 위치와 가장 유사하므로 암석의 생성 시기는 중기 백악기라고 사료되며 이들 분지가 중기 백악기에 생성된 이후 유라시아 대륙의 한 부분으로서 현재의 상대적 위치를 크게 벗어나지 않았음을 알 수 있다. This study is a paieomagnetic investigation for the Cretaceous sedimentary rocks in the Buyeo and Hampyeong basins. A total of 279 independently oriented core samples were collected from the Buyeo and Hampyeong Basins for this study. Typical stepwise AF and thermal demagnetization and measurement methods to determine the directions of characteristic remanent magnetization (ChRM) were used. The paleomagnetic pole position calculated from the mean direction for a total of 120 samples from 13 sites of the Buyeo Basin after bedding correction is at Lat./Long.=69.9˚N/193.7˚E (dp=11.5˚, dm=15.1˚), which is statistically similar to the Cretaceous pole of the Kyongsang Basin. The paleomagnetic pole position of the Hampyeong Basin calculated from the mean CWM after bedding correction for a total of 159 independently oriented samples from 20 sites in the Hampyeong Basin is at Lat/Long.=63.7˚N/205.2˚E(dp=8.8˚, dm=12.4˚), which is similar to the paleomagnetic pole (Lat/Long.=66.4˚N/204.5˚N, A%=2.8˚) of the Cretaceous rocks in the Kyongsang Basin, suggesting that the Hampyeong Basin is stable since the Cretaceous period.

Lactobacillus casei와 Lactobacillus delbrueckii간의 Protoplast 융합에 관한 연구

전홍기,김미경,백형석 부산대학교 유전공학연구소 1992 분자생물학 연구보 Vol.8 No.-

유산균주의 균주개량방법의 일환으로 protoplast fusion 방범을 사용하여 linconmycin에 내성을 나타내는 Lactobacillus casei KCTC 1121과 rifampicin에 내성을 나타내는 Lactobacillus delbrueckii JK-414의 protoplast 형성과 재생, 융합에 대한 조건 및 융합주의 생리학적 성질 등을 검토하였다. Lactobacillus casei와 Lactobacillus delbrueckii JK-414는 삼투압 안정제로 sucrose가 함유된 protoplast forming buffer에서 5μg/ml의 mutanolysin으로 42˚C, 15분간 처리했을 때 Protoplast 형성율이 높게 나타났다. L. casei와 L. delbrueckii는 대수 증식기 중반에서 protoplast가 가장 잘 형성되었으며, MRS 배지에 삼투압 안정제로 sucrose 10%, MgCl_2 6mM, CaCl_2 6mM, gelatin 2.5%를 첨가하여 만든 재생배지에서 재생효율이 가장 좋았다. 한편, L. casei와 L. delbrueckii 사이의 protoplast 융합은 40%의 PEG 4,000을 처리하였을 때 가장 양호하였으며, 그 융합 효율은 3.2×10^-4이었다. L. casei가 L. delbrueckii보다 높은 산 생성능을 보였으며 융합주 중 특히 F23, F35의 산 생성능이 우수하였다. 융합주 중 F23, F24의 protease 활성이 모균주의 protease 활성보다 높았으며, 이들 융합주의 DNA 함량은 모균주의 2배였다. Protoplast fusion between lincomycin resistant Lactobacillus casei KCTC 1121 and rifampicin resistant Lactobacillus delbrueckii JK-414 was attempted to obtain the improved strains. Protoplasts of L. casei and L. delbrueckii were produced by mutanolysin digestion at 42˚C for 15 min. L. casei cells were converted to protoplasts by treating with 5 μg/ml of mutanolysin in 20 mM HEPES buffer (pH 7.0) containing 0.75 M sucrose at the middle logarithmic growth phase. In case of L. delbrueckii 1.0M sucrose was used osmotic stabilizer. Regeneration of protoplast in both strains was efficiently accomplished on the regeneration medium containing 10% sucrose, 6mM MgCl_2, 6mM CaCl_2, and 2.5% gelatin. Protoplast fusion between L. casei and L. delbrueckii was carried out in the presence of 40% of PEG 4,000. The frequency of protoplast fusion was found to be about 3.2 × 10^_4. Acid production of L. casei was better than that of L. delbrueckii. Among fusants, F23 and F35 exhibited excellent lactic acid production. F23 and F24 exhibited the improved proteolysis compared to that of the parent strains and they had twice as much as DNA content of the parents.

의학적 병동에서의 환경 치료적 접근

전석균,이만홍 大韓神經精神醫學會 1987 신경정신의학 Vol.26 No.2

The authors conducted five times Ward Atmosphere Scale (WAS) to evaluate the therapeutic milieu according conversion from medically oriented ward to milieu therapeutic ward. With the abstracted data, was analyzed a changing process according time variable, was compared staff's perception with patient's that of ward atmosphere. Also, to grasp the problems changing in the process of change, it was practiced participant observation, analysis of recording, individual interview with staffs. The results showed that: 1) Owing to a increase of treatment activity program, went on increasing in participation both patient and staff, but have been stagnant a improvement according time variables, have been ineffective in a introducing stage. 2) About ward atmosphere, staffs perceived relatively more negative and pessimistic than patients. 3) In conversion of treatment form, appeared a inferior understanding of staff for milieu therapy, a resistance and anxiety on conversion, and remains a transitional stage rather than a fixational stage because of inharmonious communication and insufficient cooperation for Milieu Therapy.

금속 도재 수복물을 이용한 청년기 법랑질 이형성증 환자의 수복증례

김홍준,이재훈,한동후,문홍석 大韓齒科補綴學會 2012 대한치과보철학회지 Vol.50 No.3

전반적인 치아변색과 치아마모가 심한 환자들이 심미를 위해 전치부 치료를 원하는 경우가 있다. 이런 경우 법랑질 이형성증에 대해 의심해 보아야 하며 임상 검사 및 방사선 검사를 통해 확인을 해야 한다. 법랑질 이형성증으로 판단된 경우 단지 심미적 목적 하에 전치부 치료만 하는 것은 바람직하지 못하다. 본 증례는 법랑질 이형성증으로 진단된 청년기 환자에서 대부분의 치아를 금속 도재 수복물로 치료하였다. 전반적인 변색 및 마모, 치관 파절이 있었으며, 좌측 전치부와 구치부에 교차교합이 있었다. 심미적, 기능적인 개선을 목적으로 하여 치아를 수복하였다. 전치부의 교차교합은 해소하였고, 구치부의 교차교합은 해소하지 않은 채로 진행하였다. 수복 이후 환자는 심미적, 기능적으로 만족하였으며, 3개월간의 임상관찰에서 특이할 문제없이 안정적으로 유지되어 보고하고자 한다. Some patients with generalized attrition and teeth discoloration may want their anterior teeth to be treated just for esthetic improvement. Ameologenesis imperfecta, however, should be considered for such patients prior to any treatment with thorough clinical and radiographic examination. If a patient is diagnosed with amelogenesis imperfecta, the treatment on anterior teeth just for esthetic purpose is not advisable. In this case, a young man with amelogenesis imperfecta was treated with metal-ceramic restorations. The patient had generalized attrition, teeth discoloration, crown fracture, and cross-bite on the left teeth. The ultimate objective of this treatment was to enhance esthetics and masticatory function. The cross-bite on the left anterior teeth was treated with restorations, whereas the reverse horizontal overlap was maintained on the posterior. The patient was satisfied with the result esthetically and functionally, and the third month recall examination revealed no pathologic changes associated with the treatment.

연삭기용 자동 밸런싱 장치의 설계 및 평가

장홍석,최대봉,황주호,홍준희 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

The balance of high speed spindle system with high precision rotation like grinding machine is very important. Traditionally, we use trial and error method to balance the spindle. It takes much time. So we are developing the automatic balancing equipment being used in the grinding machine. The balancing head we develop is wireless. It will be used high-speed grinding machine. We use influence coefficient method to control the automatic balancer. Experiments are based on automatic and manual balancing. We perform test of the vibration filter. It helps to remove noise. The filter and experiments with automatic balancing controller show that automatic balancing control can be successfully achieved with the quick response and good stability characteristics.

ESPI를 이용한 충격손상을 받은 복합재료 내부결함의 정량평가

김경석,양광영,장호섭,지창준,윤홍석 한국공작기계학회 2003 한국공작기계학회 추계학술대회논문집 Vol.2003 No.-

Electronic Speckle Pattern Interferometry for quantitative evaluation of a impact defect inside of composite material plate are described. The impact on composite material makes inside delamination which is difficult to detect visual inspection and ultrasonic testing due to non-homeogenous structure. This paper proposes the quantitative evaluation technique of defects under real impact. Artificial defects are designed inside of composite plate for development of inspection technique and real defects under impact are inspected and compared with results of visual inspection.