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Effective transduction of osteogenic sarcoma cells by a baculovirus vector
Song, Sun U.,Shin, Seok-Hwan,Kim, Soon-Ki,Choi, Gwang-Seong,Kim, Woo-Chul,Lee, Moon-Hee,Kim, Sei-Joong,Kim, In-Ho,Choi, Mi-Sook,Hong, Young-Jin,Lee, Kwan-Hee Microbiology Society 2003 The Journal of general virology Vol.84 No.3
<P>Efficient gene delivery of a baculovirus-derived vector (BV-p53-lacZ) to a human osteogenic sarcoma cell line, Saos-2, was serendipitously found while evaluating the vector for gene delivery to human p53-null tumour cells in a previous study. Therefore, we investigated other human, rat and mouse osteogenic sarcoma and other types of tumour cell lines for transduction efficiency via baculovirus vectors containing a lacZ reporter gene under the control of either a cytomegalovirus or Rous sarcoma virus promoter. The expression of beta-galactosidase protein, assessed by X-Gal staining and beta-galactosidase ELISA, demonstrated an extremely high level of transduction efficiency in some osteogenic sarcoma cell lines, such as U-2OS, Saos-2 and Saos-LM2. These human osteogenic sarcoma cell lines showed levels of beta-galactosidase expression 5-40 times greater than HepG2 cells, which were previously thought to be the mammalian cells most susceptible to baculovirus-mediated gene delivery. The level of acetylated histone proteins in these tumour lines did not correlate well with the high level of reporter gene expression. These results strongly suggest that some osteogenic sarcoma cells are highly susceptible to baculovirus-mediated gene delivery and that a baculovirus-derived vector is an efficient gene delivery vehicle into human osteogenic sarcoma cells.</P>
정위상 및 탈위상 경사에코영상과 생체 수소 자기공명분광법을 이용한 담낭 담즙내 지질성분의 측정$^1$
신석환,조순구,김영수,우제홍,김형진,서창해,Sin, Seok-Hwan,Jo, Sun-Gu,Kim, Yeong-Su,U, Je-Hong,Kim, Hyeong-Jin,Seo, Chang-Hae 대한영상의학회 2002 대한영상의학회지 Vol.46 No.2
목적: 지질 억제 자기공명영상 기법과 수소 자기공명분광에서 측정한 지질 피크의 상대적 비율을 비교 분석하여,이 두 방법에 의한 담낭내 담즙 지질의 정량적 분석이 가능한 지를 알아보고자 하였다. 대상과 방법: 간기능 검사 및 콜레스테롤 수치가 정상이며, 초음파검사에서 담낭벽의 비후가 없고 담낭이 잘 팽창되어 있는 정상자원자 26명을 대상으로 하였다.담낭의 정위상 및 탈위상 경사에 코영상에서 담낭의 신호강도 차이를 계산하였으며, 담낭 자기공명분광 스펙트럼의 지질피크(0.8-1.6 ppm)와,2.0-2.4 ppm,3.2-3.4 ppm,3.9-4.1 ppm,5.2-5.4 ppm에서 관찰되는 피크의 넓이를 측정하였다. 전체 피크의 합에 대한 지질피크의 비율를 계산하였고, 신호강도 비율과 지질피크 비율를 비교하였다. 결과: 정위상 및 탈위상 자기공명영상에서의 신호강도비율은11.3-43.4%(평균 26 $\pm$8.9%)였고, 생체 수소 자기공명분광의 가장 현저한 피크는 지질피크(21명,92%)였으며, [ 피크0.8-1.6 ppm의 면적/모든 피크의 면적의 합] 은 0.10-0.97(평균 0.66 $\pm$0.21)였다. 신호강도 비율과 지질피크 비율의 상관성은 통계적으로 의미있는 상관성을 보였다(r=0.478,p=0.014). 결론: 지질 억제 자기공명영상 기법과 수소 자기공명분광법이 담낭내 담즙 지질성분을 측정하는 방법으로 이용될 가능성이 있다. Purpose: To evaluate the utility of signal intensity differences between in- and opposed-phase MRI and the lipid peak ratio in in-vivo proton MR spectroscopy of the gallbladder as diagnostic tools for measuring the lipidcontent of gallbladder bile. Materials and Methods: Twenty-six normal volunteers underwent MR imaging (FMPSPGR) and in-vivo proton MR spectroscopy of the gallbladder. In all cases the results of liver function tests were normal, as were cholesterol levels,and ultrasonography of the gaubladder revealed nothing unusual. For MRI and MRS a 1.5T unit (Signa Horizon; GE Medical Systems, Milwaukee, U.S.A.) was used. In-phase and opposed-phase coronal-section MR images(FMPSPGR; TR=125 msec, TE=1.8, 4.2 msec) of the gallbladder were obtained, and differences in signal intensity thus determined. For proton MR spectroscopy of the gallbladder, a localized proton STEAM sequence was employed. A single voxel of 1-8 cm3 was placed at the center of the gallbladder cavity, peak areas at 0.8-1.6 ppm (lipid), 2.0-2.4 ppm, 3.2-3.4 ppm, 3.9-4.1 ppm, and 5.2-5.4 ppm were measured by proton MRS and the relative peak area ratios of peak 0.8-1.6 ppm/other peaks were calculated. The degree of correlation between signal intensity differences at MRI and the relative peak area ratio of lipid in proton MRS was estimated using the p-value and Pearson's correlation coefficient. Results: Signal intensity differences ranged from 11.3 to 43.4% (mean,26$\pm$8.9%), and the range of lipid peak area ratio at MRS was 0.10-0.97 (mean, 0.66$\pm$0.21). There was significant correlation between the two measured values (P=0.014, Pearson’s correlation coefficient=0.478). Conclusion: In normal cystic bile, signal intensity differences at in- and opposed-phase MRI and relative lipid peak area ratios at MRS varied, though both methods could be used diagnostically for measuring the lipid contents of body tissue.
Lee, Ji-Seon,Park, Jeong-Rak,Kwon, Ok-Seon,Lee, Tae-Hee,Nakano, Ichiro,Miyoshi, Hiroyuki,Chun, Kwang-Hoon,Park, Myung-Jin,Lee, Hong Jun,Kim, Seung U.,Cha, Hyuk-Jin Oxford University Press 2015 Neuro-oncology Vol.17 No.1
<P><B>Background</B></P><P>Cancer stemness, observed in several types of glioma stem cells (GSCs), has been demonstrated to be an important barrier for efficient cancer therapy. We have previously reported that cancerous neural stem cells (F3.Ras.CNSCs), derived from immortalized human neural stem cells by a single oncogenic stimulation, form glial tumors in vivo.</P><P><B>Method</B></P><P>We searched for a commonly expressed stress modulator in both F3.Ras.CNSCs and GSCs and identified silent mating type information regulation 2, homolog (SIRT1) as a key factor in maintaining cancer stemness.</P><P><B>Result</B></P><P>We demonstrate that the expression of SIRT1, expressed in “cancer cells with neural stemness,” is critical not only for the maintenance of stem cells, but also for oncogenic transformation. Interestingly, SIRT1 is essential for the survival and tumorigenicity of F3.Ras.CNSCs and GSCs but not for the U87 glioma cell line.</P><P><B>Conclusion</B></P><P>These results indicate that expression of SIRT1 in cancer cells with neural stemness plays an important role in suppressing p53-dependent tumor surveillance, the abrogation of which may be responsible not only for inducing oncogenic transformation but also for retaining the neural cancer stemness of the cells, suggesting that SIRT1 may be a putative therapeutic target in GSCs.</P>
Protective Effects of KH-204 in the Bladder of Androgen-Deprived Rats
Woong Jin Bae,U Syn Ha,Jin Bong Choi,Kang Sup Kim,Su Jin Kim,Hyuk Jin Cho,Sung Hoo Hong,Ji Youl Lee,Zhiping Wang,Sung Yeoun Hwang,Sae Woong Kim 대한남성과학회 2015 The World Journal of Men's Health Vol.33 No.2
Purpose: We investigated the protective effects of the herbal formulation KH-204 in the bladder of androgen-deprived rats. Materials and Methods: Male rats aged eight weeks were randomly divided into four groups, containing eight rats each: sham operation only (normal control group), androgen-deprived only (androgen-deprived control group), and androgen-deprived followed by treatment with 200 mg/kg or 400 mg/kg of KH-204. After 0.5 mg/kg of leuprorelin was subcutaneously injected in the androgen-deprived groups, the oral administration of either distilled water in the two control groups or KH-204 in the treatment group was continued for four weeks. Serum testosterone levels, RhoGEF levels, nitric oxide (NO)-cyclic guanosine monophosphate (cGMP)-related parameters, oxidative stress, and histologic changes were evaluated after treatment. Results: Treatment with the herbal formulation KH-204 (1) increased serum testosterone levels; (2) restored the expression of RhoGEFs, endothelial NO synthase, and neuronal NO synthase; (3) increased the expression of superoxide dismutase; and (4) decreased bladder fibrosis. Conclusions: Our results suggest that the positive effects of KH-204 on the urinary bladder may be attributed to its antioxidant effects or to an elevation in NO-cGMP activity.
α-glucosidase inhibition of Cudrania tricuspidata according to each parts and period. (초)
( Hyeong U Son ),( Chul Hong Park ),( Jin Chul Heo ),( Dong Yoon Nam ),( Si Rim Lee ),( Hyun Jin Lee ),( Tae Yang Cha ),( Jin Hong Baek ),( Sang Han Lee ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2010 한국식품저장유통학회 학술대회 Vol.2010 No.-
Case Reports : A Case of Combined Hepatocellular-Cholangiocarcinoma with Underlying Schistosomiasis
Chang Kyun Hong,Jin Mo Yang,Bong Koo Kang,Jin Dong Kim,Young Chul Kim,U Im Chang,Jin Young Yoo 대한내과학회 2007 The Korean Journal of Internal Medicine Vol.22 No.4
Combined hepatocellular-cholangiocarcinoma is a rare form of primary liver cancer showing features of both hepatocellular and biliary epithelial differentiation. We report here on a case with collision tumor, which apparently was the coincidental occurrence of both hepatocellular carcinoma and cholangiocarcinoma underlying schistosomiasis. A 39-year-old-Philippine female was transferred to our hospital for evaluation of a liver mass that was found on ultrasonography at a local hospital. HBsAg and Anti-HCV were negative and serum alpha-fetoprotein (AFP) level was normal. The tumor mass was histologically diagnosed as adenocarcinoma by sono-guided biopsy before the operation. Partial lobectomy was performed and we histologically identified the concurrent occurrence of hepatocellular carcinoma and cholangiocarcinoma, (a "collision type carcinoma").
박진우 ( Park Jin U ),김우진 ( Kim U Jin ),신동훈 ( Sin Dong Hun ),최종수 ( Choe Jong Su ),김기홍 ( Kim Gi Hong ),김동석 ( Kim Dong Seog ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.9
Plexiform schwannoma is a rare, benign neoplasm arising from the peripheral nerve sheath and usually arises in either the dermis or subcutaneous tissue. This neoplasm occurs most commonly as a slowly growing asymptomatic solitary nodule but may occur as m
Kim, Su Jin,Jeon, Seung Hwan,Kwon, Eun Bi,Jeong, Hyun Cheol,Choi, Sae Woong,Bae, Woong Jin,Cho, Hyuk Jin,Ha, U-Syn,Hong, Sung Hoo,Lee, Ji Youl,Hwang, Sung Yeoun,Kim, Sae Woong Korean Society for Sexual Medicine and Andrology 2017 The World Journal of Men's Health Vol.35 No.3
<P><B>Purpose</B></P><P>Many patients with benign prostatic hyperplasia need treatment for remaining storage symptoms after surgery. Therefore, we evaluated the effect of the phytotherapeutic agent WSY-1075 on persistent detrusor overactivity (DO) after the relief of bladder outlet obstruction (BOO).</P><P><B>Materials and Methods</B></P><P>Rats were assigned to 3 groups: control (n=6), persistent DO (n=6), and persistent DO treated with the phytotherapeutic agent WSY-1075 (n=6). Persistent DO after relief of partial BOO was generated in the rat model, and 6 of the rats with this condition were orally administered WSY-1075. After 4 weeks of administration, cystometry was performed. Additionally, 8-hydroxy-2-deoxyguanosine and superoxide dismutase were measured to evaluate oxidative stress in the bladder. Pro-inflammatory cytokines, such as interleukin-8 and tumor necrosis factor-α, were analyzed, as were the M2 and M3 muscarinic receptors of the bladder.</P><P><B>Results</B></P><P>Significantly increased contraction pressure and a decreased contraction interval were observed in the persistent DO group after relief of BOO. Moreover, oxidative stress, pro-inflammatory cytokines, and M3 muscarinic receptors were significantly increased. After treatment with WSY-1075, significantly reduced DO was observed by cystometry in comparison with the persistent DO group. Additionally, significantly decreased levels of oxidative stress, pro-inflammatory cytokines, and M3 muscarinic receptors in the bladder were observed after treatment with WSY-1075.</P><P><B>Conclusions</B></P><P>Treatment with WSY-1075 improved persistent DO after the relief of BOO mediated by antioxidative and anti-inflammatory effects. Further studies are necessary to identify the exact mechanism of the treatment effect of WSY-1075.</P>
Reduction of matrix metalloproteinase-9 expression by culture filtrate of Paecilomyces farinosus J3
LEE, HYUN-JIN,PARK, CHUL-HONG,SON, HYUNG-U,HEO, JIN-CHUL,NAM, SUNG-HEE,LEE, KWANG-GIL,YEO, JOO-HONG,YOON, CHEOL-SIK,KIM, JONG-MYEUNG,SHIN, YONG-KYU,KIM, SI-OH,LEE, SANG-HAN Spandidos Publications 2011 Experimental and therapeutic medicine Vol.2 No.2