위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.

Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants

Hyo Shim Han,고영진,허재선,정재성 한국미생물학회 2004 The journal of microbiology Vol.42 No.4

The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAACGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gln to Arg.

Occurrence of the strA-strB Streptomycin Resistance Genes in Pseudomonas Species Isolated from Kiwifruit Plants

Han Hyo Shim,Koh Young Jin,Hur Jae-Seoun,Jung Jae Sung The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.4

The occurrence of strA-strB streptomycin-resistance genes within transposon Tn5393 was examined in Pseudomonas syringae pv. actinidiae, P. syringae pv. syringae, and P. marginalis, isolated from kiwifruit plants in Korea and Japan. PCR amplification with primers specific to strA-strB revealed that three of the tested Pseudomonas species harbored these genes for a streptomycin-resistance determinant. Tn5393, containing strA-strB, was also identified with PCR primers designed to amplify parts of tnpA, res, and tnpR. No IS elements were detected within tnpR, nor were they found in the intergenic region between tnpR and strA. Nucleotide sequence analysis indicated that the strA sequence of P. syringae pv. actinidiae contained a single nucleotide alteration at position 593 (CAA $\rightarrow$CGA), as compared to Tn5393a in P. syringae pv. syringae. This resulted in an amino acid change, from Gin to Arg.

Molecular Bases of High-Level Streptomycin Resistance in Pseudomonas marginalis and Pseudomonas syringae pv. actinidiae

Han, Hyo-Shim,Nam, Hye-Young,Koh, Young-Jin,Hur, Jae-Seoun,Jung, Jae-Sung The Microbiological Society of Korea 2003 The journal of microbiology Vol.41 No.1

We have collected eight high-level streptomycin-resistant strains of Pseudomonas marginalis and P. syringae pv. actinidiae which were isolated from kiwifruit orchards in Korea and Japan, The molecular mechanisms of resistance were investigated by the PCR, susceptibility tests, and nucleotide sequence analysis. Of the eight high-level streptomycin-resistant strains, four harbored strA-strB genes, which encode streptomycin-inactivating enzymes. While the three Korean strains of R marginalis did not have plasmid and carried the resistant genes in the chromosomes, the Japanese strain of P. syringae pv. actinidiae had a plasmid containing strA-strB genes. The myomycin susceptibility test demonstrated that the high-level resistance to streptomycin of the remaining four strains is associated with mutations in the rpsL gene. Nucleotide sequence analyses revealed that they contain a single base-pair mutation in codon 43 of their rpsL gene.

Identification and Characterization of Coronatine-Producing Pseudomonas syringae pv. Actinidiae

( Hyo Shim Han ),( Young Jin Koh ),( Jae Seoun Hur ),( Jae Sung Jung ) 한국미생물생명공학회 2003 Journal of microbiology and biotechnology Vol.13 No.1

토착미생물별 가축분 퇴비화 과정중 생물화학적 특성 변화

한효심(Hyo-Shim Han),이경동(Kyung-Dong Lee) 한국토양비료학회 2012 한국토양비료학회지 Vol.45 No.6

다양한 기능을 가진 토착미생물을 분리·동정 후 퇴비화에 적용하여 우수한 기능성을 가진 발효퇴비를 제조하고자 하였다. 7개의 우수 토착미생물을 주축으로 3개의 발효퇴비를 제조하여 생리활성을 조사한 결과, 대두의 발아율 증가, 섬유소, 키틴, 단백질 분해 등의 생리활성이 대조구보다 뛰어나고, 오옥신 생산량이 유의적으로 증가하였다. 퇴비중 암모니아 가스와 휘발성 지방산 함량 역시 2차 발효가 시작되는 20일까지 함량 감소가 있었고, 대두의 건물수량이 증가함을 확인하였다. 또한 식물보호제 분해시험에서도 유의적인 분해율이 있었고, 중금속 함량 역시 유의적으로 감소하였다. 따라서 토착미생물의 다 기능적 활성을 활용한 발효퇴비의 제조는 기존보다 더 향상된 발효퇴비를 농업생산 환경에 적용할 수 있을 것이다. In order to produce high-quality fermenting composts, bacteria strains with high activities of extracellular enzymes (cellulase, chitinase, amylase, protease and lipase) were isolated from the soils in 6 provinces of Korea, and characterized by 16S rRNA gene sequence analysis and properties. The selected 7 stains inoculated to livestock manure for 2’ fermenting time, and experimental treatment divided into 3 groups, B1, B2 and B3, according to microbial activity and enzyme type. Our results showed that microbe applications (B1, B2 and B3) can increase (p<0.05) both rhizomes (17-38%) and enzyme activities (50-81%) in compost after fermenting time, respectively, compared to non-microbe treatment (control). The microbe application also decreased significantly (p<0.05) the NH3 and H2S gas contents 13.4 and 27.3% compared with control, and the Propionic acid and Butyric acid gas contents 14.5 and 19.6%, respectively, as compared to the control. The microbial degradation rate (%) of pesticides and heavy metals increased significantly (p<0.05) after fermenting time, respectively, as compared to the control. Especially, microbe applications were more effective in total rhizomes yields and bioactivities than non-microbe treatment. Thus the results of this study could help in development of potential bioinoculants and composting techniques that maybe suitable for crop production, and protectable for earth environment under various conditions.

ITS 염기서열분석에 의한 한국산, 중국산 및 러시아산 가시오갈피의 유연관계 분석

한효심(Hyo Shim Han),김두영(Doo Young Kim),이갑연(Kab Yeon Lee),박완근(Wan Geun Park),조인경(In Kyung Cho),정재성(Jae Sung Jung) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.1

The genetic analyses of Acanthopanax senticosus Harms from Korea, China and Russia, were made by comparing the internal transcribed spacer (ITS) sequences of the nuclear ribosomal DNA. The ITS region of A. senticosus was amplified by polymerase chain reaction (PCR) using the universal primers and then directly sequenced. The length of the ITS region including 162 bp 5.85 rRNA gene ranged from 608 bp (for Korean and Chinese) to 611 bp (for Russian). The G+C content of ITS region were 60.20% for Korean and Chinese plants and 60.06% for Russian plants. Sequence comparisons indicated that ITS regions of A. senticosus from Korea and China were identical, whereas the ITS sequence of A. senticosus from Russia showed 99.2% homology with the plants from Korea. Variation in sequences were attributable to 5 bp substitution such as transversion or insertion events. These results suggested that A. senticosus Harms from Korea and China were closely related in phylogenetic relationship compared to Russian. In addition, A. senticosus Harms were more similar to Kalopanax pictus than A. sessiliflorus in their ITS sequences. 한국, 중국, 러시아에서 자생하고 있는 가시오갈피에 대한 유전적 유연관계를 파악하기 위해 ITS (internal transcribed spacer)의 염기서열을 분석하였다. Universal primer를 사용하여 PCR로 증폭시킨 후 염기서열을 결정한 결과 ITS의 길이는 162 bp의 5.8S rRNA 유전자를 포함하여 한국산과 중국산은 608 bp 그리고 러시아산은 611 bp로 나타났다. ITS영역의 G+C 함량은 한국산과 중국산이 60.20%이고 러시아산이 60.06%였다. 한국산과 중국산의 ITS영역은 100% 동일하였으며, 러시아산은 한국산과 비교했을 때 3 bp의 염기삽입과 2 bp의 염기치환이 존재하여 99.2%의 상동성을 나타내었다. 따라서 한국산 가시오갈피는 러시아산보다 중국산과 유전적 유연관계가 더 가까운 것으로 추정된다. 또한 동일 속내에서는 오갈피나무보다는 음나무와 ITS 염기서열이 유사한 것으로 나타났다.

전라남도 동부지역 오일장의 특산인 ‘국거리’ 상품에 관한 연구

한효심(Hyo Shim Han),조자용(Ja Yong Cho),허북구(Buk Gu Heo) 한국농어촌관광학회 2023 농어촌관광연구 Vol.26 No.1

연구보문 : 식물환경 ; 울금의 생육, 수량 및 품질에 미치는 퇴비의 효과

한효심 ( Hyo Shim Han ),우서 ( Seo Woo ),김동관 ( Dong Kwan Kim ),허북구 ( Buk Gu Heo ),이경동 ( Kyung Dong Lee ) 한국환경농학회 2010 한국환경농학회지 Vol.29 No.2

Curcuma longa L. (turmeric) is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout Asia etc. Recently, the demands on rhizome of turmeric are increasing greatly by well-being boom, but there is not enough to meet the demands. To fulfill increasing demands, cultivation system strategies using the organic fertilizers are required to produce a greater amount of rhizome with good quality and yield. A field experiment was conducted to determine the effect of composts, NPK fertilizer(control, F), NPK + swine manure(SM) and NPK + fermentation manure from the wasted oriental medicine materials(OMWM), on rhizome yield and the content of bioactive components for quality. Our results showed that two compost applications can increase both rhizomes(24.1-25.9%) and curcumin(21.7-41.0%) yields, respectively, compared to F control. The content of amino acids increased significantly by SM and OMWM treatments. SM and OMWM application also increased the total phenol yields 7.8 and 8.7 g/10a compared with control 6.3 g/10a, the flavonoid yields 6.3 and 7.3 g/10a compared with control 5.3 g/10a, and also antioxidant activity 21.7 and 41%, respectively, as compared to the control. Especially, OMWM was more effective in total rhizomes yields and bioactivities and in the biosynthesis of curcumin and bioactive components than SM treatments, but the biological pathway was not clear, still. This experiment suggests that curcumin or bioactive components affected by adding SM and OMWM could increase the yields and quality of turmeric.

DAF에서 압력에 따른 미세기포의 크기특성

한무영,박용효,이준,심재수 대한상하수도학회 2002 상하수도학회지 Vol.16 No.2

Although DAF (Dissolved Air Rotation) has been successfully accepted for water and wastewater treatment, the fundamental characteristics of the process have not been fully investigated. According to recent theoretical work on DAF, bubble size is one of the most important factors that affect the DAF efficiency. It is well known that the size of bubbles in DAF is mostly affected by applied pressure. In this study, a newly developed Particle Counter Method (PCM) is introduced and compared with image analysis and verified. Using this PCM, the size of bubbles in DAF is measured under various pressure conditions from 2-6atm. Through a set of experiments, it was found that bubble size decreases as the pressure increases up to 3.5atm. This is a critical pressure after which bubble size does not decrease. According to the experimental results, it is not only costly but also unnecessary to maintain pressure more than 3.5atm if the goal is only to generate smaller bubbles for better removal efficiency in DAF operational practice.