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흰쥐 해마박편에서 veratrine과 고농도 포타슘자극시 칼슘이온이 glutamic acid 유리에 미치는 영향에 관한 연구 : A role of calcium
조무길,김형룡,김관식 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2
Present study was performed to clarify the effect of calcium on the release of glutamic acid employing hippocampal slices. Hippocampal slices(300∼400㎛ thick) were prepared by the method of Kim et al.(1988) and pre-equilibrated in Krebs-bicarbonate medium(KBM, pH 7.4) for 1hr at 37℃. In case of veratrine induced glutamic acid release, pre-equilibrated slices were incubated in fresh KBM and then veratrine(25μM)-containing KBM for 10 min period in the presence or absence of 2.5mM Ca^++. In case of potassium-induced glutamic acid release, pre-equilibrated slices were incubated in fresh KBM and then potassium(50mM)-containing KBM for 5 min period in the presence or absence of 2.5mM Ca^++. Basal and veratrine and potassium-induced release of glutamic acid was determined from recovered medium by HPLC. The observed results were as follows: 1. The release of glutamic acid induced by the 10 min-exposure of 25μM veratrine and 5 min-exposure of 50mM potassium in the presence of 2.5mM Ca^++ was 330.9±25 nmol and 129.6±20.6 nmol, respectively. When compared with released amounts of glutamic acid during the corresponding spontaneous periods, these were 7.8 and 5.1-fold increase respectively. 2. the release of glutamic acid induced by the 10 min-exposure of 25μM veratrine and 5 min-exposure of 50mM potassium in the absence of Ca^++ was 361.8±31.9 nmol and 62.5±7.5 nmol, respectively. When compared with released amounts of glutamic acid during the corresponding spontaneous periods, these were 8.2 and 2.4-fold increase respectively.
고소현,박관수,강길남,방병욱,김동일 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1
This study was carried out to examine the effect of heavy traffic on chemical properties of soil in roadside trees of Daejeon city. Soil samples at 0~10cm and 10~30cm depths were collected from soil of the roadside trees, Platanus occidentalis. and Ginko biloba. Soil pH in heavy traffic regions were around 7.0 at 0~10 cm and 10~30cm soil depths because of spraying of calcium chloride for snow moving. The concentrations of Fe, Cd, Cu, Zn, and Pb in soil were higher in heavy traffic regions(Daejeon Station and Daehwa Industrial Complex) than in light traffic region(Chungnam National University). The result could be from rubbing and wear of car tire and metals when they travel.
하악골 절제술후 재이식된 돌결자가골 재이식 : 치험 3례
김 식,김수관,김운규,김수민,류종희,이준길,조경안,김수흥,정태영,여환호 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.2
We described three cases of immediate reimplantation of a frozen-thawed autogenous mandible composed of a mixture of iliac bone, marrow, and particulate hydroxyapatite in tumors of the mandible. Acceptable outcomes were obtained in three patients who underwent immediate autogenous mandibular graft reconstruction. The conditions leading to successful outcome of the procedure are also discussed. Reimplantation of frozen autogenous lesioned mandible was performed in three patients with mandibular tumors. Two reimplanted grafts survived without complications following surgery. One case had postoperative infection that resolved with appropriate antibiotic treatment. There were no recurrences of the primary lesions. Satisfactory facial contour after surgery was achieved. These results are most promising, and we believe that, with further refinement, this technique will offer a new and acceptable modality for facial reconstruction in patients with cancer.
장길식 ( Gil Sik Jang ),김연희 ( Yun Hee Kim ),최영섭 ( Young Seop Choi ),김승호 ( Seung Ho Kim ),김종민 ( Jong Min Kim ),배석진 ( Seok Jin Bae ),조영관 ( Young Gwan Cho ),구태형 ( Tae Hyoung Koo ) 한국환경농학회 2016 한국환경농학회지 Vol.35 No.2
BACKGROUND: The objective of this study is to evaluate the soil quality to cultivate crops in urban garden. METHODS AND RESULTS: During the period of six month from March to August 2015, measured eight heavy metals, seven Organic items and Fluorine on seventeen urban sites and thirty-one suburban sites in Gwangju city. METHODS AND RESULTS: The average concentration and range of heavy metal in soil are 0.15 mg/kg(ND-0.6) for Cd, 14.9 mg/kg(1.5-33.3) for Cu, 4.4 mg/kg(0.4-71.8) for As, 0.05 mg/kg(ND-1.366) for Hg, 24.7 mg/kg(13.1-62.7) for Pb, 102.5 mg/kg(49.1-276.4) for Zn and 9.2 mg/kg(ND-90.1) for Ni but Cr6+ is not detected. The average value and range of soil fertility items are 253.5mg/kg(76.6-1766.0) for fluorine, 6.4(4.8-7.7) for pH, 20.3 g/kg(5.0-44.0) for orangic matters, 562.7 mg/kg (28.0-1672.0) for available phosphate, 0.6 cmol+/kg (0.1-2.3) for K, 9.7 cmol+/kg (2.7-22.0) for Ca, 3.0 cmol+/kg (0.9-7.4) for Mg, 1.0 ds/m(0.2-2.9) for conductivity. The concentration of Hg in Suburban area is 0.005 mg/kg lower than 0.134 mg/kg in urban area. Also, the concentration of As, Cd, Ni and Zn is lower than urban area as 32%, 37%, 51%, 71% respectively. CONCLUSION: According to SPI index of soil contamination, 39 sites are first degree and 8 sites are second degree and 1 site(41th) is fourth degree. Pb and Cd are not detected and As is detected tiny amounts in plants grown polluted soil, so heavy metals have not moved to plants.
쌀의 과학과 건강 : 화학/분석/생리활성 분야 ; 열무 중 살충제의 잔류농약 분해 특성 연구
김준형 ( Kim Jun Hyeong ),서혜영 ( Seo Hye Yeong ),최근영 ( Choe Geun Yeong ),정양모 ( Jeong Yang Mo ),박순연 ( Park Sun Yeon ),김관수 ( Kim Gwan Su ),최택열 ( Choe Taeg Yeol ),손길선 ( Son Gil Seon ),김경수 ( Kim Gyeong Su ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 국제학술심포지움 쌀박람회 Vol.2003 No.-
Lee, Hyun-Gwan,Kim, Hye Mi,Min, Juhee,Kim, Keunyong,Park, Myung Gil,Jeong, Hae Jin,Kim, Kwang Young The Korean Society of Phycology 2017 ALGAE Vol.32 No.3
To quantify the abundance of the harmful dinoflagellate Cochlodinium polykrikoides in natural seawaters, we developed the innovative procedure using a droplet digital PCR (ddPCR) with C. polykrikoides-specific primers targeting the internal transcription sequence (ITS). The abundance of C. polykrikoides was estimated by the specific copy number of target ITS DNA segments per cell in cultures and natural water samples. The copy number per C. polykrikoides cell as acquired by ddPCR was $157{\pm}16$, which was evaluated against known cell numbers through a simplified protocol preparing DNAs. The abundances of C. polykrikoides in the waters of different locations estimated by ddPCR agreed with the number of cells visually counted under a microscope. This protocol was used to measure the abundance of C. polykrikoides close to and further off the southern coast of Korea in August of 2016 and 2017. The practical application showed that this method can reduce time for analysis and increase accuracy.