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Geng Hou,De-Guang Shang,Lin-Xuan Zuo,Lin-Feng Qu,Ming Xia,Yi-Er Guo,Xiang Yin,Shao-Dong Wu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.1
Fatigue test of a needled C/SiC composite with a notch under tension-tension cyclic loading was completed, and the main fatigue crack propagation curve of the needled composite was obtained by the in situ observation of the fatigue process. By analyzing the influence of the failure number and distribution on the tensile loading subjected by 0° fiber bundles, the relationship between the main fatigue crack propagation and the distribution of 0° fiber bundles in the needled composite was established. By observing the fracture microstructure (especially the distribution of 0° fiber bundles) of the needled composite through scanning electron microscopy, the reasons for the varying fatigue resistance of different notched specimens were also explained. In addition, acoustic emission (AE) was also used to analyze the AE energy characteristics during the fatigue crack propagation process of the needled composite.
Hou, Wenpeng,Zhao, Ning-Jiu,Meng, Dongli,Tang, Jing,Zeng, Yi,Wu, Yu,Weng, Yangziwan,Cheng, Chungui,Xu, Xiulai,Li, Yi,Zhang, Jian-Ping,Huang, Yong,Bielawski, Christopher W.,Geng, Jianxin American Chemical Society 2016 ACS NANO Vol.10 No.5
<P>The installation of heterojunctions on the surfaces of carbon nanotubes (CNTs) is an effective method for promoting the charge separation processes needed for CNT-based electronics and optoelectronics applications. Conjugated polymers are proven state-of-the-art candidates for modifying the surfaces of CNTs. However, all previous attempts to incorporate conjugated polymers to CNTs resulted in unordered interfaces. Herein we show that well-defined chains of regioregular poly(3-hexylthiophene) (P3HT) were successfully grown from the surfaces of multiwalled CNTs (MWNTs) using surface-initiated Kumada catalyst-transfer polycondensation. The polymerization was found to proceed in a controlled manner as chains of tunable lengths were prepared through variation of the initial monomer-to-initiator ratio. Moreover, it was determined that large-diameter MWNTs afforded highly ordered P3HT aggregates, which exhibited a markedly bathochromically shifted optical absorption due to a high grafting density induced planarization of the polymer chains. Using ultrafast spectroscopy, the heterojunctions formed between the MWNTs and P3HT were shown to effectively overcome the binding energy of excitons, leading to photoinduced electron transfer from P3HT to MWNTs. Finally, when used as prototype devices, the individual MWNT-g-P3HT core-shell structures exhibited excellent photoresponses under a low illumination density.</P>
Temperature-dependent oviposition model of Scopula subpunctaria (Lepidoptera: Geometridae)
Geng Shubao,Hou Heli,Wang Guojun,Jung Chuleui,Yin Jian,Qiao Li 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3
Scopula subpunctaria, is one of important defoliating insect pests of tea plants in China. However, the effect of temperature on its longevity and fecundity had not been reported, and the temperature-dependent oviposition model is lacking. The reproductive characteristics of S. subpunctaria were investigated under eight constant temperatures (13, 16, 19, 22, 25, 28, 31, and 33℃), with a 14L: 10D photoperid and 65–85% RH. The results showed that temperature significantly influence oviposition period, longevity, and fecundity of S. subpunctaria. The mean longevity of female adults increased from 10.8 days (33℃) to 33.9 days (13℃). Male lived shorter than female did. The mean longevity of males was shortest at 33℃ (8.1 days) and longest at 23.8℃ (17.6 days). Total fecundity of S. subpunctaria females ranged from 15.4 eggs at 33℃ to 279.9 eggs at 22℃. The temperaturedependent oviposition model of S. subpunctaria was constructed based on four sub-models (developmental rate model, total fecundity model, oviposition rate model, and survival rate model). The results of this study would be helpful in forecasting and predicting population occurrence of S. subpunctaria in tea plantations.
LFT Modeling and Robust Stability Analysis of Missiles with Uncertain Parameters
HOU Zhen-qian,LIANG Xiao-geng,WANG Wen-zheng,LI Rui 한국항공우주학회 2014 International Journal of Aeronautical and Space Sc Vol.15 No.2
The structured singular value (μ) analysis based method has many advantages for the robust stability analysis of missiles with uncertain parameters. Nevertheless, the present linear fractional transformation (LFT) modeling process, which is the basis of μ analysis, is complex, and not suitable for automatic implementation; on the other hand, μ analysis requires a large amount of computation, which is a burden for large-scale application. A constructive procedure, which is computationally more efficient, and which may lead to a lower order realization than existing algorithms, is proposed for LFT modeling. To reduce the calculation burden, an analysis method is developed, based on skew μ. On this basis, calculation of the supremum of μ over a fixed frequency range converts into a single skew μ value calculation. Two algorithms are given, to calculate the upper and lower bounds of skew μ, respectively. The validity of the proposed method is verified through robust stability analysis of a missile with real uncertain parameters.
Jiguang Hou,Dong Ren,Yufeng Chen,Lindan Geng,Shuangquan Yao,Haotian Wu,Pengcheng Wang 대한척추신경외과학회 2023 Neurospine Vol.20 No.1
Objective: This study aimed to examine the effect of the endplate reduction (EPR) technique combined with bone grafting for treating thoracolumbar burst fractures using posterior shortsegmental fixation. Methods: Patients with thoracolumbar fractures admitted between January 2018 and October 2021 were retrospectively analyzed, and those meeting the criteria were assigned to the EPR group and the intermediate screws (IS) group. The vertebral wedge angle (VWA), Cobb angle (CA), anterior vertebral body height (AVBH), middle vertebral body height (MVBH), upper endplate line (UEPL), upper intervertebral angle (UIVA), and upper intervertebral disc height (UIDH) indices were examined and compared preoperatively, first day postoperatively, as well as at 12 months postoperatively. Results: The result indicated that the EPR group achieved better MVBH reduction (p < 0.001), UEPL reduction (p < 0.001), vertebral body fracture healing (p = 0.006), as well as implant breakage (p = 0.04) than the IS group; VWA (p < 0.001), CA (p = 0.005), AVBH (p < 0.001), MVBH (p < 0.001), UEPL (p < 0.001), and UIDH (p < 0.001) were lost after reduction less than those in the IS group. There was no significant difference in operative time (p = 0.315) and intraoperative bleeding (p = 0.274) between the 2 groups. Conclusion: The EPR group achieved better results in repositioning and maintaining MVBH and endplate morphology, with less correction loss after the reduction of the VWA, CA, AVBH, and endplate morphology. The EPR group exhibited a better healing pattern after vertebral fracture and disc degeneration was better relieved.
Tao Geng,Yuxia Huang,Chengxiang Hou,Guangxing Qin,Dingding Lv,Xijie Guo 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.3
Innate immunity is critical to insects and plays an important role in pathogen elimination and wound healing. Toll signaling pathway is the major signaling pathway associated with insect innate immunity mediating synthesis of anti-fungal/bacterial peptides. To better understand the Toll signaling pathwaymediated immune response in Bombyx mori against Beauveria bassiana infection, expression patterns of genes encoding sixteen putative components of Toll signaling pathway in the silkworm larvae challenged with B. bassiana, including four pattern recognition receptors (PRRs, i.e. BmβGRP 1, 2, 3, 4), eight Toll-like receptors (TLRs, i.e. Bm18w, BmToll 1, 3, 6, 9, 7, 10, 11) and four effectors (BmMoricin 1, BmGloverin 2, BmDefensin 1 and BmLysozyme 1), were analyzed using quantitative real-time PCR. At the same time, the changes in their expression by RNAi knock-down of the four PRRswere also detected.Moreover, the effects of Toll signaling pathway inhibitors on antifungal activity in larvae hemolymphwere also analyzed. The results showed that the expression levels of genes encoding sixteen putative components of Toll signaling pathway were obviously altered by the challengewith B. bassiana, but their temporal regulation mode was significantly different. Based on the expression patterns of the genes related to Toll signaling pathway, two sub-paths of immune signal recognition and transduction might be proposed in the response of silkworm larvae against B. bassiana infection. Besides, Toll signaling pathway inhibitor could significantly inhibit the antifungal activity in hemolymph and resulted in increased sensitivity of silkworm larvae to the B. bassiana infection, while the treatment with heat-inactivated B. bassiana could induce antifungal activity in the hemolymph and led to stronger resistance of the silkworm. These results implied that Toll signaling pathway played important roles in the antifungal immune response of the silkworm larvae, in which different components of Toll signaling pathway might play a specific regulatory function. These findings yield insights into the innate immune mechanisms underlying Toll signaling pathway in silkworm.
Kou, Geng,Gao, Jie,Wang, Hao,Chen, Huaiwen,Li, Bohua,Zhang, Dapeng,Wang, Shuhui,Hou, Sheng,Qian, Weizhu,Dai, Jianxin,Zhong, Yanqiang,Guo, Yajun Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
The purpose of this study was to develop paclitaxel-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles coated with cationic SM5-1 single-chain antibody (scFv) containing a polylysine (SMFv-polylys). SM5-1 scFv (SMFv) is derived from SM5-1 monoclonal antibody, which binds to a 230 kDa membrane protein specifically expressed on melanoma, hepatocellular carcinoma and breast cancer cells. SMFv-polylys was expressed in Escherichia coli and purified by cation-exchange chromatography. Purified SMFv-polylys was fixed to paclitaxel-loaded PLGA nanoparticles to form paclitaxel-loaded PLGA nanoparticles coated with SMFv-polylys (Ptx-NP-S). Ptx-NP-S was shown to retain the specific antigen-binding affinity of SMFv-polylys to SM5-1 binding protein-positive Ch-hep-3 cells. Finally, the cytotoxicity of Ptx-NP-S was evaluated by a non-radioactive cell proliferation assay. It was demonstrated that Ptx-NP-S had significantly enhanced in vitro cytotoxicity against Ch-hep-3 cells as compared with non-targeted paclitaxel-loaded PLGA nanoparticles. In conclusion, our results suggest that cationic SMFv-polylys has been successfully generated and may be used as targeted ligand for preparing cancer-targeted nanoparticles.
Can Du,Zengchao Geng,Qiang Wang,Tongtong Zhang,Wenxiang He,Lin Hou,Yueling Wang 한국미생물학회 2017 The journal of microbiology Vol.55 No.9
Microbial communities in subsurface soil are specialized for their environment, which is distinct from that of the surface communities. However, little is known about the microbial communities (bacteria and fungi) that exist in the deeper soil horizons. Vertical changes in microbial alpha-diversity (Chao1 and Shannon indices) and community composition were investigated at four soil depths (0–10, 10–20, 20–40, and 40–60 cm) in a natural secondary forest of Betula albosinensis by high-throughput sequencing of the 16S and internal transcribed spacer rDNA regions. The numbers of operational taxonomic units (OTUs), and the Chao1 and Shannon indices decreased in the deeper soil layers. Each soil layer contained both mutual and specific OTUs. In the 40–60 cm soil layer, 175 and 235 specific bacterial and fungal OTUs were identified, respectively. Acidobacteria was the most dominant bacterial group in all four soil layers, but reached its maximum at 40–60 cm (62.88%). In particular, the 40–60 cm soil layer typically showed the highest abundance of the fungal genus Inocybe (47.46%). The Chao1 and Shannon indices were significantly correlated with the soil organic carbon content. Redundancy analysis indicated that the bacterial communities were closely correlated with soil organic carbon content (P = 0.001). Collectively, these results indicate that soil nutrients alter the microbial diversity and relative abundance and affect the microbial composition.