국내산 무화과의 식중독균에 대한 항균활성

정미란,차정단,이영은 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

The antibacterial activities of methanol extract and systematic solvent fractions(-hexane, chloroform, ethyl acetate, and butanol) from Korean common type figs at different ripening stages were tested by the broth dilution method against 8 representative food-poisoning bacteria- : L. monocytogenes, S. aureus, S. enteritidis, E. cols 0157:H7, E. cols, Y. enterocolitico, V. parahaemolvticus, and S. ttphimurium. The methanol extracts of unripened Ⅰ and Ⅱ showed stronger activity than that of the ripened figs especially against L. monocytogenes, S. enteriridis, E. toll 0157:H7, V. parahaemolvticus and S. nphimurium in 10 mg/mL. The systematic solvent fractions showed stronger antibacterial activities than the methanol extract, even at the lower concentrations. The hexane fraction of ripened Figs showed higher growth inhibition than those of unripened Ⅰ and Ⅱ against L. monocytogenes, E. colt 0157.117, Y. enterocolitica and V. parahaemolvtieus. The chloroform fraction showed strong antibacterial activity in all ripening stages against E. soli 0157:H7 and V. parahaemolyticus. The butanol fraction showed better inhibition activity in unripened I and II than in the ripened figs. The hexane and chloroform fractions showed inhibition activity of more than 75% against E. coli 0157:117, V. parahaemohvicus in 0.5 mg/mL. Each fraction showed a little different antibacterial activity according to the ripening stages of the fruits and the tested strains. Especially, rigs in the unripened II stage revealed superior activity relatively and the hexane and chloroform tractions revealed the strongest activity, followed by the butanol fraction, while the ethylacetare and water fractions hardly showed any activity.

치주질환 진단시 프랙탈 분석의 유용성에 관한 연구

차상윤,한원정,김은경 대한구강악안면방사선학회 2001 Imaging Science in Dentistry Vol.31 No.1

Purpose : To evaluate the usefulness of fractal analysis for diagnosis of periodontitis. Materials and Methods : Each 30 cases of periapical films of male mandibular molar were selected in normal group and patient group which had complete furcation involvement. They were digitized at 300 dpi,256 gray levels and saved with gif format. Rectangular ROIs (10×20 pixel) were selected at furcation, interdental crest, and interdental middle 1/3 area. Fractal dimensions were calculated three times at each area by mass radius method and were determined using a mean of three measurements. We compared fractal dimensions at furcation and interdental crest area of normal group with those of patient group. And then we compared ratio of fractal dimensions at furcation area, interdental crest area to interdental middle 1/3 area. Results : Fractal dimension at interdental crest area of normal group was 1.979±0.018 and that of patient group 1.971±0.012 (p〉0.05). Fractal dimension at furcation area of normal group was 1.986±0.024 and that of patient group 1.974±0.015 (p〈 0.05). The ratio of fractal dimension at interdental crest area to interdental middle 1/3 of normal group was 1.003±0.015 and that of patient group 0.993±0.018 (p〈0.05), The ratio of fractal dimension at furcation area to interdental middle 1/3 of normal group was 1.006±0.018 and that of patient group 0.994±0.018 (p〈 0.05). Conclusion : The ratio of fractal dimension at interdental crest and furcation area to interdental middle 1/3 area showed a statistically significant difference between normal and patient group. In conclusion, it is thought that fractal analysis might be useful for the diagnosis of periodontitis.(Korean J Oral Maxillofac Radiol 2001;31:35-42)

Koji를 이용한 저식염 멸치젓의 속성제조에 관한 연구

차용준,김은정,주동식 國立昌原大學校 基礎科學硏究所 1994 基礎科學硏究所論文集 Vol.6 No.-

산업적으로 경쟁력이 있는 젓갈을 제조하기 위한 목적으로 본 연구는 현재까지 연구된 저식염 젓갈에 Koji를 첨가하여 젓갈의 속성제조 및 풍미성분의 개량을 시도하여 보았다. Koji를 첨가한 저식염 멸치젓은 숙성 30일경에 완전히 숙성되었으며 이때 아미노질소 함량도 가장 높았다. 지방 및 단백질 분해효소의 활성은 대조구 및 Koji첨가 시료 모두 숙성 20일 및 30일경에 가장 높았고 그 후로 감소하였다. 단백질 분해균은 Koji의 경우 숙성 20일경에, 대조구는 숙성 30일경에 가장 많았으며 그 후로 감소하였다. Koji를 첨가한 저식염 멸치젓은 재래식 기존 젓갈에 비해 단기간에 숙성시킬 수 있었다. The adding effect of Koji, made by Aspergillus oryzae, in low salt-fermented anchovy paste was studied to reduce fermentation time and to enhance flavor quality as a commercial feasibilty in seafood industry. The content of volatile basic nitrogen increased slowly during fermentation, whereas that of amino nitrogen was the highest in 30days of low salt-fermented anchovy made with Koji. Protease and lipase activity in anchovy paste, which made with or without Koji, were high amount in 30 and 20 days of fermentation, respectively. A number of proteolytic bacteria was high level in 13 days and then slowly decreased afterward.

국내산 무화과를 이용한 와인 제조 및 품질 개선 효과

정미란,차정단,윤순일,한종현,이영은 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

Fig(Ficus carica L.) which belongs to Moraceae family has been used as digestion promoter, cure for ulcerative inflammation and eruption in Korea. Figs have very short preservation period and can be kept only 1 week even in the refrigeration system. The possibility of wine-making from Korean figs was tested by analyzing the fermentation characteristics, proximate analysis and free sugar contents of wine made of slices with 20% sugar addition. Ethanol fermentation of the figs by Saccharomyces cerevisiae was done rapidly. The ethanol contents, pH, total acidity and sugar content were 12.1%, 3.91, 0.42% and 9.9 °Brix, respectively. The results from the sensory evaluation were as follows. The wine made from the sliced figs were better than that from fig juices in their sensory scores. The wine made with frozen figs was not different from that made with fresh ones. To improve the quality of wine, the addition of dried leaf powder in the concentration of 100 ppm were tried and found it was superior to the one made without leaf.

단클론성 IgG 침착을 동반한 증식성 사구체 신염 1예

차정민,정덕은,송주흥,안선호 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Proliferative glomerulonephritis with monoclonal IgG deposits is a novel phenotype of glomerular injury with the diagnostic criteria of (1) the presence of glomerular monoclonal IgG deposits restricted to a single IgG subclass and a single light chain isotype, associated with endocapillary proliferative, membranoproliferative, or membranous features; (2) the presence of granular("immune complex type") deposits by electron microscopy; and (3) the absence of clinical and laboratory evidence of cryoglobulinemia. We report a case of 65-year-old man with generalized edema who was diagnosed as proliferative glomerulonephritis with monoclonal IgG deposits by renal biopsy.

구름버섯과 영지버섯의 기능성에 관한 연구

차은정,황영정,김성훈 한국가정과학회 2004 한국가정과학회지 Vol.7 No.2

The purpose of this study was to investigate the physiological functionality of Coriolus versicolor Quel(CV) and Ganoderma lucidum (Fr.)Karst (GL) by estimate the proximate composition and content of antioxidant components. 1. In the physicochemical property, the content of proximate composition of GL was higher (18.28% of moisture, 10.3% of c rude protein, 78.4% of crude fiber) than that of CV, but the content of crude lipid and ashes of GL was higher than that of CV. 2. CV had relatively higher content of antioxidant components such as total phenol, carotenoids and v itamin C, than GL did. Antioxidant mineral components such as magnesium and zinc also had relatively higher in CV than in GL. 3. TBA value, conjugated diene production, LDL oxidation, DPPH of methanol extracts in CV and GL were as high as α-ocopherol.

MALT 림프종을 수반한 쉐그렌 증후군

한원정,차상윤,김은경 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.2

Sj□gren's syndrome is a chronic inflammatory disease that predominantly affects salivary, lacrimal and other exocrine glands. We report a case of Sj□gren's syndrome combined with MALT (mucose associated lymphoid tissue) Iymphoma which occured in the parotid gland. A 57-year-old female with the complaint of painful swelling and iymph node enlargement was referred to our department. Sialograms of both parotid glands showed globular collections of contrast material uniformly distributed throughout the parotid gland. Salivary scintigraphy showed decreased uptake of the parotid gland. CT scan showed larger, slightly more dense parotid gland than normal and honeycomb glandular appearance. Also, It showed discrete, slightly more enhanced round mass in the left parotid gland. Histopathological finding showed replacement of salivary gland parenchyma with dense small lymphocytic infiltration having the feature of epimyoepithelial islands. Kappa light chain restriction of interglandular plasma cell could be seen. (Korean J Oral maxillofac Radiol 2000; 30: 144-148)

멀티미디어기술을 이용한 치과방사선학 전자 교과서 제작에 관한 연구

김은경,차상윤,한원정,홍병희 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.1

Purpose : This study was performed to develop the electronic textbook (CD-rom title) about preclinical practice of oral and maxillofacial radiology, using multimedia technology with interactive environment. Materials and Methods : After comparing the three authoring methods of multimedia, i.e, programming language, multimedia authoring tool and web authoring tool, we determined the web authoring tool as an authoring method of our electronic textbook. Intel Pentium Ⅱ 350 MHz IBM-compatible personal computer with 128 Megabyte RAM, Umax Powerlook flatbed scanner with transparency unit, Olympus Camedia 1400L digital camera, ESS 1686 sound card, Sony 8 mm Handycam, PC Vision 97 pro capture board, Namo web editor 3.0, Photoshop 3.0, ThumbNailer, RealPlayer 7 basic and RealProducer G2 were used for creating the text document, diagram, figure, X-ray image, video and sound files. We made use of javascripts for tree menu structure, moving text bar, link button and spread list menu and image map etc. After creating all files and hyperlinking them, we burned out the CD-rom title with all of the above multimedia data, Netscape communicator and plug in program as a prototype. Results and Conclusions : We developed the dental radiology electronic textbook which has 9 chapters and consists of 155 text documents, 26 figures, 150 X-ray image files,20 video files,20 sound files and 50 questions with answers. We expect that this CD-rom title can be used at the intranet and internet environments and continuous updates will be performed easily. (Korean J Oral Maxillofac Radiol 2000; 30: 55-62)

SDE 및 V-SDE법에 의한 굴가수분해물의 휘발성 성분 비교

김 훈,차용준,장성민,김은정 창원대학교 생활과학연구소 1997 생활과학연구 Vol.- No.1

Volatile flavor compounds in oyster hydrolysate were compared by means of SDE(simultaneous steam distillation-solvent extraction) and vacuum SDE(V-SDE)/GC/MSD methods. A total of 68 volatile flavor compounds in oyster hydrolysate was identified by the both methods(68 in SDE and 57 in V-SDE method). These compounds were composed of 16 aldehydes, 17 ketones, 10 alcohols, 13 nitrogen containing compounds, and 15 miscellaneous compounds including sulfur containing compounds(3) an aromatic hydrocarbonds(4). Levels of aldehydes and N-containing compounds (e.g. alkylpyrazines) obtained from SDE method were higher than from V-SDE method, whereas levels of alcohols decreased than in V-SDE method. Lots of thermal generation aroma were produced in SDE than in V-SDE.

급성 연쇄상구균 감염후 사구체 신염에 동반된 고혈압 뇌증 1례

하태선,차상훈,이경은,엄정민 충북대학교 의과대학 충북대학교 의학연구소 1999 忠北醫大學術誌 Vol.9 No.2

고혈압성 뇌병증은 고혈압 환자에서 고혈압과 관련된 두통, 경련, 시각장애 및 다른 신경학적 장애를 동반하는 증후군이다. 증례는 13세 여자환아로 급성 연쇄상구균 감염후 사구체 신염에 동반된 고혈압성 뇌병증 환자였다. 내원시 혈압은 150/110 mmHg 였고 내원당시 검사소견상 단백뇨, 혈뇨, C3의 감소 소견을 보였다. 내원 2일째 자기공명영상을 시행하였고 스핀에코 T2 강조영상에서 양 후두엽과 후 측두엽에 고신호의 이상 부위들이 발견되었다. 치료후 실시한 자기공명영상은 정상 소견을 보였다. 본 저자들은 급성 사구체 신염 환자에서 자기공명영상으로 고혈압성 뇌병증을 진단하고 치료한 1례를 문헌고찰과 함께 보고하는 바이다. Hypertensive encephalopathy is a syndrome consisting of headache, seizures, visual changes, and other neurologic disturbances in patients with elevated systemic blood pressure. We experienced a 13-year-old girl who presented with the manifestations of hypertensive encephalopathy in acute post-streptococcal glomerulonephritis. She visited our hospital because of generalized tonic-clonic seizure after severe headache. On admission her blood pressure was 150/110 mmHg and initial laboratory findings revealed proteinuria, hematuria and decreased C3 level and, T2-weighted MR image showed increased T2 signal in both occipital lobes, suggesting cerebral edema. On follow-up MRI, pathologic lesions were improved. We described a case of hypertensive encephalopathy in acute post-streptococcal glomerulonephritis diagnosed by MRI with a brief review of some related literatures.