Estabilishment of BALB / c Mice Model Infected With Helicobacter pylori

(Dong Zhu Jin),(Hyun Chae Jung),(Jung Mogg Kim),(Joo Sung Kim),(In Sung Song),(Chung Yong Kim) 대한내과학회 1999 The Korean Journal of Internal Medicine Vol.14 No.2

N/A Objectives:Considering the geographic differences in the prevalence of virulence factors such as CagA or VacA of H. pylori isolated from Korean adults compared with those from western countries, the establishment of a mouse model infected with H. pylori isolated from Korean adults is needed to investigate the pathogenesis and to develop vaccines against H. pylori infection in Korea. The aim of this study was to establish the BALB/c mouse model infected with H. pylori isolated from Korean. Methods:Six-week-old BALB/c mice were inoculated intragastrically with 109 CFU of H. pylori. Loss of glandular architecture, erosions and infiltration of inflammatory cells within the lamina propria compared with normal gastric mucosa were scrutinized. Evidence for H. pylori infection was assessed by rapid urease test of gastric mucosa and by microscopic examination using the H & E stain and Warthin-Starry silver stain. Results:Rapid urease test was positive in 55% of all inoculated mice. Definite histologic changes and the evidence of H. pylori colonization were observed in the H. pylori infected group. Significant infiltration of inflammatory cells was observed 6 weeks after the last inoculation and the level of serum IgG against H. pylori was increased from 2 weeks after the last inoculation. Conclusions:The H. pylori isolated freshly from Korean adults could colonize the stomach of BALB/c mice and induce pathologic alterations that mimics human gastric diseases. This model would facilitate the investigations for the pathogenetic mechanisms of H. pylori infection.

Hepatocyte growth factor and soluble cMet levels in plasma are prognostic biomarkers of mortality in patients with severe acute kidney injury

( Lilin Li ),( Jung Nam An ),( Jeonghwan Lee ),( Dong Jin Shin ),( Shi Mao Zhu ),( Jin Hyuk Kim ),( Dong Ki Kim ),( Dong-ryeol Ryu ),( Sejoong Kim ),( Jung Pyo Lee ) 대한신장학회 2021 Kidney Research and Clinical Practice Vol.40 No.4

Background: Hepatocyte growth factor (HGF)/cMet pathway is necessary for repair and regeneration following acute kidney injury (AKI). We evaluated the clinical potential of plasma HGF and soluble cMet as prognostic biomarkers for severe AKI requiring continuous renal replacement therapy (CRRT). Methods: One hundred thirty-six patients with severe AKI who participated in the VENUS (volume management under body composition monitoring in critically ill patients on CRRT) trial between 2017 and 2019 were enrolled in this study. We investigated associations between plasma HGF and cMet concentrations and all-cause mortality. Results: Plasma HGF and soluble cMet levels were positively correlated. Patients were divided into three groups based on their HGF and soluble cMet concentrations. The day D 0, D2, and D7 highest concentration HGF groups had significantly higher in-hospital mortality after adjusting for sex, body mass index, Acute Physiology and Chronic Health Evaluation II, and age-adjusted Charlson comorbidity index score, especially on D7 (hazard ratio, 4.26; 95% confidence interval, 1.71-10.62; p = 0.002). D7 soluble cMet level was also associated with mortality. Receiver operating characteristic curve analysis indicated that D7 HGF and soluble cMet levels were best at predicting mortality. Addition of plasma HGF and soluble cMet to conventional prognostic indices significantly improved the predictive value for mortality on D7. However, plasma HGF and soluble cMet were not associated with fluid status. Conclusion: Plasma HGF and soluble cMet levels were significant predictors of the outcomes of severe AKI patients undergoing CRRT. There was no correlation between plasma HGF and soluble cMet levels and fluid balance.

Morphological defense of the egg mass of Spodoptera frugiperda (Lepidoptera: Noctuidae) affects parasitic capacity and alters behaviors of egg parasitoid wasps

Dong Hui,Zhu Kai-hui,Zhao Qian,Bai Xue-ping,Zhou Jin-cheng,Zhang Li-sheng 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

The fall armyworm, Spodoptera frugiperda, is a dangerous pest of cereals originating from the tropical and sub tropical parts of the Americas. It has invaded over a hundred countries and is spreading rapidly throughout East Asia. Biological control programs are a “model strategy” for the control of invasive pests. Egg parasitoids Tele nomus remus and Trichogramma pretiosum are viewed as candidates for the control of S. frugiperda. The aim of this study was to evaluate the behavioral patterns and parasitism efficacy of Te. remus, Tr. pretiosum, and Tricho gramma dendrolimi on S. frugiperda egg masses with and without scales. The proportion of parasitism by Te. remus was significantly higher than that by Tr. dendrolimi and Tr. pretiosum. The proportion of parasitism by Tr. pre tiosum and Tr. dendrolimi on egg masses with scales was significantly lower than that on egg masses without scales. However, Te. remus had a similar proportion of parasitism on egg masses with and without scales. Residence time, oviposition time, oviposition frequency, risk of host being found, and risk of parasitism by Te. remus were significantly higher than the corresponding parameters of Tr. pretiosum and Tr. dendrolimi. Tr. pre tiosum females exhibited a higher residence time, oviposition time, oviposition frequency, risk of host being found, and risk of parasitism than those of Tr. dendrolimi females. In addition, Te. remus females often crept into the scale layer covering the egg masses, whereas Tr. dendrolimi and Tr. pretiosum females did not; they could only parasitize the eggs located on the periphery of the egg mass. Both Te. remus and Tr. pretiosum females had similar proportions of superparasitism, which were significantly higher than that of Tr. dendrolimi. Therefore, Te. remus is the dominant egg parasitoid of S. frugiperda and this has important implications for developing augmentative biological control strategies for S. frugiperda.

Isolation and characterization of tick-borne Roseomonas haemaphysalidis sp. nov. and rodent-borne Roseomonas marmotae sp. nov.

Zhu Wentao,Zhou Juan,Lu Shan,Yang Jing,Lai Xin-He,Jin Dong,Pu Ji,Huang Yuyuan,Liu Liyun,Li Zhenjun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Four novel Gram-negative, mesophilic, aerobic, motile, and cocci-shaped strains were isolated from tick samples (strains 546T and 573) and respiratory tracts of marmots (strains 1318T and 1311). The 16S rRNA gene sequencing revealed that strains 546T and 573 were 97.8% identical to Roseomonas wenyumeiae Z23T, whereas strains 1311 and 1318T were 98.3% identical to Roseomonas ludipueritiae DSM 14915T. In addition, a 98.0% identity was observed between strains 546T and 1318T. Phylogenetic and phylogenomic analyses revealed that strains 546T and 573 clustered with R. wenyumeiae Z23T, whereas strains 1311 and 1318T grouped with R. ludipueritiae DSM 14915T. The average nucleotide identity between our isolates and members of the genus Roseomonas was below 95%. The genomic G+C content of strains 546T and 1318T was 70.9% and 69.3%, respectively. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the major polar lipids, with Q-10 as the predominant respiratory quinone. According to all genotypic, phenotypic, phylogenetic, and phylogenomic analyses, the four strains represent two novel species of the genus Roseomonas, for which the names Roseomonas haemaphysalidis sp. nov. and Roseomonas marmotae sp. nov. are proposed, with 546T (= GDMCC 1.1780T = JCM 34187T) and 1318T (= GDMCC 1.1781T = JCM 34188T) as type strains, respectively.

Perforation optimization of hydraulic fracturing of oil and gas well

Zhu, Hai Yan,Deng, Jin Gen,Chen, Zi Jian,An, Feng Chen,Liu, Shu Jie,Peng, Cheng Yong,Wen, Min,Dong, Guang Techno-Press 2013 Geomechanics & engineering Vol.5 No.5

Considering the influences of fluid penetration, casing, excavation processes of wellbore and perforation tunnels, the seepage-deformation finite element model of oil and gas well coupled with perforating technique is established using the tensile strength failure criterion, in which the user-defined subroutine is developed to investigate the dynamic evolvement of the reservoir porosity and permeability. The results show that the increases of perforation angle and decreases of perforation density lead to a higher fracture initiation pressure, while the changes of the perforation diameter and length have no evident influences on the fracture initiation pressure. As for initiation location for the fracture in wellbore, it is on the wellbore face while considering the presence of the casing. By contrast, the fractures firstly initiate on the root of the tunnels without considering casing. Besides, the initial fracture position is also related with the perforation angle. The fracture initiation position is located in the point far away from the wellbore face, when the perforation angle is around $30^{\circ}$; however, when the perforation angle is increased to $45^{\circ}$, a plane fracture is initiated from the wellbore face in the maximum horizontal stress direction; no fractures was found around perforation tunnels, when the angel is close to $90^{\circ}$. The results have been successfully applied in an oilfield, with the error of only 1.1% comparing the fracture initiation pressure simulated with the one from on-site experiment.

A Study on the Development of Wind and Wave Model of Typhoon

Jin Guo-Zhu,Song Chae-Uk,Seol Dong-Il Korean Institute of Navigation and Port Research 2004 한국항해항만학회지 Vol.28 No.9

In this paper, after analyzing other models with their advantages and disadvantages, we proposed a simple parametric model for calculating wind speed & direction and wave height & direction at any location around the typhoon at sea. The proposed wind-field model of typhoon is asymmetric, and consists of a circular symmetric wind-field caused by the pressure gradient of stationary typhoon and a moving wind-field caused by the movement of typhoon. By verifying this model through observed data, we found that it is accurate enough to develop the simulation software for training students and seafarers so as to take appropriate actions while being faced with the typhoon at sea.

Increase of Intracellular $Ca^{2+}$ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells

Zhu, Mei-Hong,Park, Sung-Jin,Kim, Hyun-Jin,Yang, Dong-Ki,Suh, Suk-Hyo,So, In-Suk,Kim, Ki-Whan The Korean Society of Pharmacology 2002 The Korean Journal of Physiology & Pharmacology Vol.6 No.2

Effects of oxidized low-density lipoprotein (ox-LDL), $1-{\alpha}-stearoyl-lysophosphatidylcholine$ (LPC), on intracellular $Ca^{2+}$ concentration were examined in mouse endothelial cells by measuring intracellular $Ca^{2+}$ concentration $([Ca^{2+}]_i)$ with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased $[Ca^{2+}]_i$ under the condition of 1.5 mM $[Ca^{2+}]_o$ but did not show any effect under the nominally $Ca^{2+}-free$ condition. Even after the store depletion with $30{\mu}M$ 2,5-di-tert- butylhydroquinone (BHQ) or $30{\mu}M$ ATP, LPC could still increase the $[Ca^{2+}]_i$ under the condition of 1.5 mM $[Ca^{2+}]_o.$ The time required to increase [$Ca{2+}$]i (about 1 minute) was longer than that for ATP-induced $[Ca^{2+}]_i$ increase $(10{\sim}30\;seconds).$ LPC-induced $[Ca^{2+}]_i$ increase was completely blocked by $1{\mu}M\;La^{3+}.$ Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased $[Ca^{2+}]_i$ via the increase of $Ca^{2+}$ influx through the $Ca^{2+}$ routes which exist in the plasma membrane.

Hydraulic fracturing experiments of highly deviated well with oriented perforation technique

Zhu, Hai Y.,Deng, Jin G.,Liu, Shu J.,Wen, Min,Peng, Cheng Y.,Li, Ji R.,Chen, Zi J.,Hu, Lian B.,Lin, Hai,Guang, Dong Techno-Press 2014 Geomechanics & engineering Vol.6 No.2

In order to investigate the effect of different perforation angles (the angle between the perforation direction and the maximum horizontal principal stress) on the fracture initiation and propagation during hydraulic fracturing of highly deviated well in oil & gas saturated formation, laboratory experiments of the hydraulic fracturing had been carried out on the basis of non-dimensional similar criteria by using 400^3 $mm^3$ cement cubes. A plane fracture can be produced when the perforations are placed in the direction of the maximum horizontal principal stress. When the perforation angle is $45^{\circ}$, the fractures firstly initiate from the perforations at the upper side of the wellbore, and then turn to the maximum horizontal principal stress direction. When the well deviation angle and perforation angle are both between $45^{\circ}$ and $90^{\circ}$, the fractures hardly initiate from the perforations at the lower side of the wellbore. Well azimuth (the angle between the wellbore axis and the maximum horizontal principal stress) has a little influence on the fracture geometries; however it mainly increases the fracture roughness, fracture continuity and the number of secondary fractures, and also increases the fracture initiation and propagation pressure. Oriented perforating technology should be applied in highly deviated well to obtain a single plane fracture. If the well deviation angle is smaller, the fractures may link up.

Antifungal Mechanism of Antifungal Peptide Derived from Cecropin A(1-8)-Melittin(1-12) Hybrid against Aspergillus fumigatus

(Dong Gun Lee),(Zhe Zhu Jin),(Cheol Young Maeng),(Song Yub Shin),(Moo Yeol Seo),(Kil Lyong Kim),(Kyung Soo Hahm) 한국미생물생명공학회 1999 Journal of microbiology and biotechnology Vol.9 No.2

N/A N/A

Structure - Antifungal Activity Relationships of Cecropin A-Magainin2 and Cecropin A-Melittin Hybrid Peptides on Pathogenic Fungal Cells

( Dong Gun Lee ),( Song Yub Shin ),( Kil Lyong Kim ),( Kyung Soo Hahm ),( Joo Hyun Kang ),( Zhe Zhu Jin ) 한국미생물생명공학회 1998 Journal of microbiology and biotechnology Vol.8 No.6