무우의 雄性不稔性에 對한 遺傳因子 分析

Do Hyon Lee(李度鉉) 한국육종학회 1985 한국육종학회지 Vol.17 No.2

Using spontaneous male sterile individual of radish, cultivar Euisung-banchung, the analysis of genetic mechanism of male sterility was attempted. MS lines, in general, had small floral organs, late flowering time and yellowish floral buds, which varied among different lines. Pollen sterility was caused by the interactions of plasmagene(S) and a single pair of homo recessive karyogene(msms). The two genotypes, Smsms and Nmsms, make it possible to induce and maintain the male sterility of radish. The discovery of male sterile individuals seemed to be prevented by the presence of few individuals with S cytoplasm, though the individuals of Nmsms were prevalent in natural population of radishes.

Reticulate pigmented anomaly of the flexures(Dowling-Degos disease)

( Hyon Joo Lee ),( Sung Hoon Lee ),( Seok Jong Lee ),( Do Won Kim ),( Sang Lip Chung ) 대한피부과학회 1999 초록집 Vol.37 No.20

Flavonoids Isolated from Korea <i>Citrus aurantium</i> L. Induce G2/M Phase Arrest and Apoptosis in Human Gastric Cancer AGS Cells

Lee, Do-Hoon,Park, Kwang-Il,Park, Hyeon-Soo,Kang, Sang-Rim,Nagappan, Arulkumar,Kim, Jin-A,Kim, Eun-Hee,Lee, Won-Sup,Hah, Young-Sool,Chung, Hyon-Jong,An, Su-Jin,Kim, Gon-Sup Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P><I>Aim of the Study</I>. Citrus species is used in traditional medicine as medicinal herb in several Asian countries including Korea. Flavonioids became known as various properties, such as anti-oxidants, anti-inflammation and anti-cancer, and so forth. The present study, the anti-cancer effect of flavonioids isolated from <I>Citrus aurantium</I> L. in human gastric cancer AGS cells has been investigated. <I>Materials and Methods</I>. The anti-proliferative activity was assayed using MTT assay. Cell cycle analysis was done using flow cytometry and apoptosis detection was done using by hoechst fluorescent staining and Annexin V-propidium iodide double staining. Western blot was used to detect the expression of protein related with cell cycle and apoptosis. <I>Results</I>. Flavonoids isolated from <I>Citrus aurantium</I> L. have the effect of anti proliferation on AGS cells with IC50 value of 99 <I><I>μ</I></I>g/mL. Flavonoids inhibited cell cycle progression in the G2/M phase and decrease expression level of cyclin B1, cdc 2, cdc 25c. Flavonoids induced apoptosis through activate caspase and inactivate PARP. <I>Conclusions</I>. Flavonoids isolated from <I>Citrus aurantium</I> L. induced G2/M phase arrest through the modulation of cell cycle related proteins and apoptosis through activation caspase. These finding suggest flavonoids isolated from <I>Citrus aurantium</I> L. were useful agent for the chemoprevention of gastric cancer.</P>

Characteristics of Eggshell Powder as Carriers of Probiotics

Woo-Do Lee(이우도),Kai-Min Niu(우개민),Jeong-Min Lim(임정민),Kwon-Jung Yi(이권정),Bong-Joo Lee(이봉주),Kang-Woong Kim(김강웅),Kyoung-Duck Kim(김경덕),Sang-Woo Hur(허상우),Hyon-Sob Han(한현섭),Soo-Ki Kim(김수기) 한국생명과학회 2018 생명과학회지 Vol.28 No.1

계란 가공 부산물인 난각(ES: Eggshell)은 탄산칼슘 함량이 높아 사료에 첨가하여 칼슘원으로 이용되고 있다. 본 연구에서는 ES를 생균제의 부형제인 운반체로서 활용 가능성을 처음으로 시도하였다. L. plantarum을 대두박(SBM: Soybean meal), 난각조각(ESL: Eggshell powder with large particles), 난각미세분말(ESF: Eggshell powder with fine particles), 그리고 이들의 복합운반체인 SBM+ESL과 SBM+ESF에 생균제를 흡착시켜 그 부착상태를 주사전자현미경으로 확인하였다. 이 중 복합운반체인 SBM+ESF는 상온에서 4주 동안 pH 7~8을 유지하면서 L. plantarum의 가장 높은 생존율을 보였다. 본 연구에 사용한 모든 생균제들은 보존기간 동안 4℃에서는 높은 생존율을 보였다. 30℃에서는 유산균수는 크게 감소하였으나, B. licheniformis는 높은 생존율을 유지하였고 B. subtilis, B. amyloliquefaciens와 S. cerevisiae는 2 log10 (CFU/g)정도 감소하였다. 상기 연구결과는 사료의 칼슘원으로 이용되는 난각미세분말(ESF)을 대두박과 혼합하여 사용하면 B. licheniformis를 비롯한 일부 생균제의 생존성을 향상시켜 부형제(운반체)로도 사용할 수 있음을 밝혔다. Eggshell (ES) is a by-product of table eggs with high content of calcium carbonate which can be used as a calcium source in feed. In this study, we have first illuminated the potential application of ES as a novel carrier for probiotics. The carriers used in the study include a SBM (Soybean meal), ESL (Eggshell powder with large particles), ESF (Eggshell powder with fine particles), and the complex carriers (SBM+ESL, SBM+ESF). The structure of carriers absorbed by L. plantarum was confirmed by SEM image. Among these carriers, the complex carrier SBM+ESF showed the highest viability of L. plantarum with pH 7~8 during four weeks storage at room temperature. The SBM+ESF was further tested as a carrier for various probiotic strains at 4℃ or 30℃. All the probiotic strains showed high viability at 4℃ storage. However, a significant reduction of Lactobacillus cells was observed at 30℃ storage. B. lichenifomis maintained high viability whereas B. subtilis, B. amyloliquefaciens, and S. cerevisiae showed the reduction of 2 log10 (CFU/g). These results suggest that if the ESF as a calcium source in feed was mixed with SBM, it can be used as an effective complex carrier for improving the viability of some probiotics including B. licheniformis.

황폐계류의 사방공작물 시공에 따른 저서성 대형무척추동물상의 변화 -시공 직후의 변화를 중심으로

이도형 ( Do Hyung Lee ),이기환 ( Ki Hwan Lee ),이헌호 ( Heon Ho Lee ),마호섭 ( Ho Seop Ma ),배관호 ( Kwan Ho Bae ),김종현 ( Jong Hyon Kim ) 한국환경생태학회 2009 한국환경생태학회지 Vol.23 No.4

본 연구는 산지황폐계류에 시공한 사방공작물이 계곡생태계에 미치는 영향을 분석하고자 시공 전후를 기준으로 조사지점별로 저서성 대형무척추동물의 출현종의 변화분석 및 생물학적 수질평가를 실시하였으며, 그 결과는 다음과 같다. 시공 전 출현한 저서성 대형무척추동물의 총 수는 4문 6강 11목 22과 25종 3,086개체였으나 시공 후 조사에서는 4문 6강 10목 17과 19종 1,208개체로 감소하였다. 특히, 직접적인 사방공작물의 시공이 이루어진 조사지점에서는 시공 후 생물종이 전혀 출현하지 않거나, 극소량만이 채집되어 서식종의 현존량을 기초로 하는 다양도 지수, 풍부도 지수, 균등도 지수가 감소 또는 산출되지 않았다. 저서생물의 군집분석 결과 및 저서성 대형무척추동물의 생태점수(ESB)는 모든 조사지점에서 시공전보다 시공 후에 오수생물계열, 환경상태, 수질등급과 같은 계곡환경질이 저하된 것으로 나타났다. 즉, 시공전 상류는 양호 및 다소 양호, 중류는 다소 불량, 하류는 불량 및 매우 불량으로 분석되었으나, 시공 후 상류 일부 지역을 제외한 나머지 수역은 매우 불량으로 분석되었다. 또한 대상 계류의 수질등급의 경우 시공 전에는 I~III등급 사이로 분류되었으나, 시공 후 대조군을 제외하고는 모두 IV~V등급으로 악화되었다. 이와 같이 사방공작물의 시공후 모든 조사지점에서 발생한 저서성 대형무척추동물의 서식 환경 파괴는 개체수의 감소와 수질악화로 이어졌다. 이 결과는 사방공작물의 시공 직후에 조사 분석한 것이므로, 향후 장기간의 모니터링을 통하여 시공한 사방공작물의 재해방지 효과와 함께 계류 생태계의 회복과정에 대한 지속적인 연구가 이루어져야 할 것이다. This study was conducted to evaluate the effect of erosion control works on the stream ecosystem in a mountain torrential stream. The species composition of benthic macroinvertebrates and biological water quality were investigated before and after construction of erosion control works at 5 sites. The total number of benthic macroinvertebrates before construction was 3,086 individuals including 4 phyla, 6 classes, 11 orders, 22 families, and 25 species, but after construction it has decreased to 1,208 individuals including 4 phyla, 6 classes, 10 orders, 17 families, and 19 species. Especially, the diversity, richness, and evenness indices, which were calculated based on existing number of biological species, have decreased or not produced at the erosion control sites, where biological species were not found or the minimum number of species were found. The results of community analysis of benthic macroinvertebrates and ESB(Ecological Score of Benthic macroinvertebrate community) indicated that the environmental qualities of the stream based on saprobity, environmental condition, and water quality decreased after the construction at all sites: before construction, the top of the stream was satisfactory and some satisfactory, the middle was some defectiveness, and the lower was defectiveness and very defectiveness; after construction, all parts of the stream except some parts of the top were very defectiveness. Moreover, the water quality of torrential stream was rated between I to III before construction, but after construction, it declined to IV~V except control. The habitat damage of benthic macroinvertebrates occurred at all investigation sites after the construction of erosion control works led to reduction of the number of biological species and water quality deterioration. The results reported in this study were collected directly after the construction of erosion control works. Therefore, additional studies are needed to further explore the effect of disaster-prevention of erosion control works and the recovery process of stream ecosystem through long term monitoring.

Effect of Home-based Self-administered Transcranial Direct Stimulation in Patients with Mild to Moderate Major Depressive Disorder: A Single-arm, Multicentral Trial

Jihoon Oh(Jihoon Oh),Sekye Jeon(Sekye Jeon),Tae Hyon Ha(Tae Hyon Ha),Woojae Myung(Woojae Myung),Seung-Hwan Lee(Seung-Hwan Lee),Young-Hoon Ko(Young-Hoon Ko),Do Hoon Kim(Do Hoon Kim),Hwa-Young Lee(Hwa-Y 대한정신약물학회 2023 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.21 No.2

Objective: Although the effects and safety of transcranial direct current stimulation (tDCS) treatment in depressive patients are largely investigated, whether the self-administration of tDCS treatment at patient’s home is comparable to clinic-based treatment is still unknown. Methods: In this single-arm, multi-center clinical trial, 61 patients with mild to moderate major depressive disorder were enrolled. tDCS treatment was delivered at the patient’s home once a day, 5 to 7 times a week for 6 weeks, and each session lasted for 30 minutes. The primary outcome was a total Beck-Depression Inventory-II score, and no concurrent antidepressants were used. Results: The remission rates in both Full-Analysis (FA) (n = 61) and Per-Protocol (PP) (n = 43) groups were statistically significant (FA: 57.4% [0.44−0.70], PP: 62.8% [0.47−0.77]; percent [95% confidence interval]). The degree of depression- related symptoms was also significantly improved in 2, 4, and 6 weeks after the treatment when compared with baseline. There was no significant association between treatment compliance and remission rate in both FA and PP groups. Conclusion: These results suggest that acute treatment of patient-administered tDCS might be effective in improving the subjective feeling of depressive symptoms in mild to moderate major depressive disorder patients.

촛점 모니터를 갖는 레이저 사진술 장치

이도형,송재원,손현 경북대학교 전자기술연구소 1990 電子技術硏究誌 Vol.11 No.1

This paper represents the construction of laser beam writing system. laser lithography. using new method that guarantees convenience and accuracy in laser focusing. The X, Y translation stage using DC motors was controlled by the computer. Minimum Ire width of 1.6㎛ was obtained by the laser lithography system.

Hard x-ray nano patterning using a sectioned multilayer

Lee, Su Yong,Cho, In Hwa,Kim, Jae Myung,Yan, Hanfei,Conley, Ray,Liu, Chian,Macrander, Albert T.,Maser, Jö,rg,Stephenson, G. Brian,Kang, Hyon Chol,Noh, Do Young American Institute of Physics 2011 Journal of Applied Physics Vol.109 No.4

X-ray-induced synthesis of Au nanoparticles on ZnO nanowire arrays

Lee, Mu Sung,Lee, Su Yong,Noh, Do Young,Kang, Hyon Chol Elsevier 2019 Materials chemistry and physics Vol.229 No.-

<P><B>Abstract</B></P> <P>We report the synchrotron X-ray-induced synthesis of plasmonic Au nanoparticles (NPs) on ZnO nanowire (NW) arrays. By X-ray irradiation at room temperature, Au NPs were synthesized from an aqueous solution containing HAuCl<SUB>4</SUB> and NaHCO<SUB>3</SUB>. Samples prepared in continuous and scanning exposure modes were examined by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy and Raman spectroscopy. In the continuous exposure mode, a match-like AuZnO structure was dominantly observed. We found that the scanning mode is suitable for the synthesis of monodisperse Au NP-ZnO NW heterostructures. The average diameter of the Au NPs was approximately 11.8 nm. The peak intensity of the E<SUB>2</SUB>(high) mode doubled and the <SUP>2</SUP>E<SUB>2</SUB> peak can be clearly observed in Raman spectrum of Au NP-ZnO NW heterostructure. These changes are evidence of the surface plasmon resonance effect due to the Au NPs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Synchrotron x-ray induced synthesis of Au nanoparticles on ZnO nanowire array was investigated. </LI> <LI> Scanning exposure mode was suitable for obtaining a dispersed Au NP-ZnO NW heterostructure. </LI> <LI> The peak intensity of the Raman spectrum increased by a factor of two because of surface plasmon resonance. </LI> </UL> </P>

Loss of toll-like receptor 3 aggravates hepatic inflammation but ameliorates steatosis in mice

Lee, Young-Sun,Kim, Do-Yeon,Kim, Tae-Jun,Kim, So Yeon,Jeong, Jong-Min,Jeong, Won-Il,Jung, Jae-Kwang,Choi, Jae-Kap,Yi, Hyon-Seung,Byun, Jin-Seok Elsevier 2018 Biochemical and biophysical research communication Vol.497 No.4

<P><B>Abstract</B></P> <P>The importance of toll-like receptor (TLR) 4 in the pathogenesis of steatohepatitis has been well documented; however, little is known about the role of TLR3. In this study, we determined whether the depletion of TLR3 modulated hepatic injury in mice and further aimed to provide mechanistic insights into the TLR3-mediated modulation of diet-induced hepatic inflammation and fat accumulation. Hepatic steatosis and inflammatory response were induced by feeding wild-type (WT) or TLR3 knockout mice a high-fat diet for 8 weeks. Primary liver resident cells, including hepatocytes, Kupffer cells, and hepatic stellate cells (HSCs), were treated with palmitic acid. TLR3 knockout mice fed a high-fat diet showed severe hepatic inflammation accompanied by nuclear factor-κB and IRF3 activation, which is mainly induced by the activation of Kupffer cells. Decreased TLR4 expression was restored in hepatic mononuclear cells and Kupffer cells in TLR3 knockout mice compared to that in the WT. Moreover, hepatic steatosis was decreased in TLR3 knockout mice. Hepatocytes from TLR3 knockout mice exhibited reduced expression of cannabinoid receptors. HSCs from TLR3 knockout mice showed decreased expression of the enzymes involved in endocannabinoid synthesis. In conclusion, this study suggests that the selective modulation of TLR3 could be a novel therapeutic target for the treatment of hepatic inflammation and steatosis.</P> <P><B>Graphical abstract</B></P> <P>Suggested diagram of TLR3 mediated-changed TLR4 signaling is responsible for increased inflammation and decreased steatosis in TLR3 knockout mice.</P> <P>[DISPLAY OMISSION]</P>