돼지 H-FABP 유전자의 다형성 및 경제 형질과의 연관성 구명

최봉환,김태헌,이지웅,조용민,이혜영,조병욱,정일정 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

The purpose of this study was to detect association between genetic variation and economic trait in the porcine heart type fatty acid-binding protein gene as a candidate gene for the traits related with growth and meat quality in pigs. The H-FABP is a 15-kDa protein expressed in several tissues with high demand for fat metabolism such as cardiac and skeletal muscle and lactating mammary gland. H-FABP is small intracellular protein involved in fatty acid transport from the plasma membrane to the site of β-oxidation and/or triacylglycerol or phospholipid synthesis. In this study, H-FABP PCR-RFLP was performed in F_(2) population composed of 214 individuals form an intercross between Korean Native Boars and Landrace sows. PCR products form tow primer sets within H-FABP gene were amplified in 850bp and 700bp. Digestion of PCR products with the restriction digestion enzymes HaeⅢ and Hinf Ⅰ, revealed fragment length polymorphisms(RFL. Ps). The genotype frequencies from H-FABP/HaeⅢ was .29 for genotype DD, .53 for genotype Dd, and .15 for genotype dd, respectively. The genotype frequencies of HH, Hh, and hh from H-FABP(hinf Ⅰ was .38, .41, and .20, respectively, in the population.Relationships between their genotypes and economic traits were estimated. In H-FABP/HaeⅢ locus, there were specific genotypes(Dd and dd) associated with economic traits such as body weight. In H-FABP/Hinf Ⅰ Iocus, Genotypes of HH and Hh associated with growth traits such as body weights at 5, 12, and 30 week of age (p<.05 or p<.001) and back fat thickness, body fat including abdominal and trimmed fat (p<.001) and intramuscular fat(p<.05). The 'H'allele was positivecly associated with gaining of body weight and fatness deposition. In conclusion, a significant association of the H-FABP gene from its genetic variation was found on body weight, intramuscular fat and backfat thickness.

Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection

Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3

Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.

Attenuation of oxidative neuronal cell death by coffee phenolic phytochemicals

Cho, E.S.,Jang, Y.J.,Hwang, M.K.,Kang, N.J.,Lee, K.W.,Lee, H.J. Elsevier 2009 Mutation research Vol.661 No.1

Neurodegenerative disorders such as Alzheimer's disease (AD) are strongly associated with oxidative stress, which is induced by reactive oxygen species (ROS) including hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Recent studies suggest that moderate coffee consumption may reduce the risk of neurodegenerative diseases such as AD, but the molecular mechanisms underlying this effect remain to be clarified. In this study, we investigated the protective effects of chlorogenic acid (5-O-caffeoylquinic acid; CGA), a major phenolic phytochemical found in instant decaffeinated coffee (IDC), and IDC against oxidative PC12 neuronal cell death. IDC (1 and 5μg/ml) or CGA (1 and 5μM) attenuated H<SUB>2</SUB>O<SUB>2</SUB>-induced PC12 cell death. H<SUB>2</SUB>O<SUB>2</SUB>-induced nuclear condensation and DNA fragmentation were strongly inhibited by pretreatment with IDC or CGA. Pretreatment with IDC or CGA also inhibited the H<SUB>2</SUB>O<SUB>2</SUB>-induced cleavage of poly(ADP-ribose) polymerase (PARP), and downregulation of Bcl-X<SUB>L</SUB> and caspase-3. The accumulation of intracellular ROS in H<SUB>2</SUB>O<SUB>2</SUB>-treated PC12 cells was dose-dependently diminished by IDC or CGA. The activation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) by H<SUB>2</SUB>O<SUB>2</SUB> in PC12 cells was also inhibited by IDC or CGA. Collectively, these results indicate that IDC and CGA protect PC12 cells from H<SUB>2</SUB>O<SUB>2</SUB>-induced apoptosis by blocking the accumulation of intracellular ROS and the activation of MAPKs.

Enhanced PCR efficiency of high-fidelity DNA polymerase from Thermococcus waiotapuensis

Cho, S.S.,Yu, M.,Kim, S.H.,Kwon, S.T. IPC Science and Technology Press ; Elsevier Scienc 2014 Enzyme and microbial technology Vol.63 No.-

Twa DNA polymerase from hyperthermophilic archaeon Thermococcus waiotapuensis has exceedingly high fidelity among family B DNA polymerases. However, Twa DNA polymerase has significant shortcomings in terms of a low extension rate and poor processivity. To resolve these weaknesses, we focused on two amino acid residues (N565 and H633) in the palm and thumb subdomains of the Twa DNA polymerase. These two residues were replaced by site-directed mutagenesis and the enzymatic properties of the mutants were analyzed. Here, Twa H633R DNA polymerase showed significantly improved polymerase function compared to wild-type Twa DNA polymerase in terms of processivity (2-fold), extension rate (1.5-fold) and PCR efficiency. Kinetic analysis using DNA as a template revealed that the k<SUB>cat</SUB> value of the Twa H633R mutant was similar to that of wild-type, but the K<SUB>m</SUB> of the Twa H633R mutant was about 1.6-fold lower than that of the wild-type. These results showed that the Arg residue substitution at H633 located in the thumb subdomain has a positive effect on processivity, extension rate and PCR efficiency, suggesting that the Twa H633R mutant allows a conformational change for easy access of the primer-template to the binding site of the polymerase domain.

Aluminizing and Boroaluminizing Treatments of Mar-M247 and Their Effect on Hot Corrosion Resistance in Na₂SO₄-NaCl Molten Salt

Cho, J.H.,Kim, T.W.,Son, K.S.,Yoon, J.H.,Kim, H.S.,Leisk, G.G.,Mitton, D.B.,Latanision, R.M. 대한금속재료학회 2003 METALS AND MATERIALS International Vol.9 No.3

The effect of surface modifications of Mar-M247 superalloy on hot corrosion resistance was examined in Na₂SO₄-NaCl molten salt. The Mar-M247 was aluminized and boroaluminized by pack cementation in Ar and underwent a cyclic hot corrosion test in Na₂SO₄-NaCl molten salt. The XRD results showed that a Ni2Al3 phase was formed between the aluminized layer and the substrate when the surface modification temperature was below 1273 K. However, a NiAl phase formed when the temperature was above 1273 K. The intensity of the XRD peak in the NiAl phase increased after post heat treatment. Hot corrosion resistance increased for the specimens containing NiAl rather than Ni₂Al₃ phase. The ductile NiAl phase suppressed the potential for crack initiation during thermal cycling. Post heat treatment increased the corrosion resistance of the aluminized layer for Mar-M247, which underwent surface modification at 1273 K and above. In the boroaluminized Mar-M247 specimens, corrosion resistance decreased as a result of the blocking of outward diffusion of Cr by boron and decreased cohesion between the oxide scale and the aluminized layer during thermal cycling.

돼지의 선발에 있어서 능력검정 방법에 따른 육종가 및 유전적 개량량의 변화

조규호,김시동,김명직,이일주,전광주 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.6

본 연구에서는 이유시체중(㎏), 90㎏ 도달일령을 대상형질로 하여 폐쇄돈군에서 이유시 체중을 기초로 5가지의 선발방법에 따라 선발된 선발축의 산육 능력을 검정하여 검정이 완료된 개체들에 대해 유전능력 평가를 실시한후 검정대상 선발두수의 크기에 따른 유전적 개량량을 비교하여 이상적인 검정방법을 찾고자 실시하였다. 시뮬레이션 결과 90㎏ 도달일령에 대한 육종가 추정치의 변화는 Method Ⅴ그룹에서 -36.2일의 단축효과를 보였으며 세대당 유전적 개량량 또한 Method Ⅴ그룹에서 -7.5일로 가장 높은 개량을 보였다. 따라서 개량적인 측면에 있어서는 전수검정을 실시하는 것이 가장 좋을 것으로 사료되지만 농장의 검정능력이나 전수검정을 실시할 때 드는 초과비용에 대한 경제적인 분석은 더 연구되어져야 될 것으로 사료된다. This study was carried out to find the ideal testing method by comparing predicted breeding values, and genetic gain by the size of testing group. Tested traits were weaning weight and days to 90㎏. Five methods for selecting testing group were adopted: Method Ⅰ (MⅠ ; 30% selected based on litter weight at weaning for female, and 30% selected based on individual weight at weaning for mail), Method Ⅱ (MⅡ ; 30% selected based on individual weight at weaning for female and male, respectively), Method Ⅲ (MⅢ ; 50% selected based on individual weight at weaning for female and male, respectively), Method Ⅳ (MⅣ ; 70% selected based on individual weight at weaning for female and male, respectively), Method Ⅴ (MⅤ ; all piglets were tested). Genetic ability was evaluated by multiple trait animal model using MTDFREML package. Based on the predicted breeding values for days to 90㎏, the next generation was selected up to 5th generation. The above procedure were repeated and simulated by 4 times. The results obtained in the present study are summarized as follows; Phenotypic mean and predicted breeding values mean of days to 90㎏ was 114.3, -27.8, 113.7, -27.0, 107.5, -32.6, 105.7, -34.4, 103.7 and -36.2 days for MⅠ, MⅡ, MⅢ, MⅣ and MⅤ, respectively. Phenotypic and predicted breeding values variance of days to 90㎏ was 20.9, 4.1, 19.0, 3.4, 15.3, 1.8, 13.8, 1.7, 13.3 and 1.2 days for MⅠ, MⅡ, MⅢ, MⅣ and MⅤ, respectively. Genetic gain of days to 90㎏ was -6.2, -6.0, -7.1, -7.3 and -7.5 days for MⅠ, MⅡ, MⅢ, MⅣ and MⅤ, respectively. In all case, Method Ⅴ group, all piglets be tested, is the most efficient for improving the target economic traits.

Novel Ag@TiO₂ nanocomposite synthesized by electrochemically active biofilm for nonenzymatic hydrogen peroxide sensor

Khan, M.M.,Ansari, S.A.,Lee, J.,Cho, M.H. Elsevier 2013 Materials science & engineering. C, Materials for Vol.33 No.8

A novel nonenzymatic sensor for H<SUB>2</SUB>O<SUB>2</SUB> was developed based on an Ag@TiO<SUB>2</SUB> nanocomposite synthesized using a simple and cost effective approach with an electrochemically active biofilm. The optical, structural, morphological and electrochemical properties of the as-prepared Ag@TiO<SUB>2</SUB> nanocomposite were examined by UV-vis spectroscopy, X-ray diffraction, transmission electron microscopy and cyclic voltammetry (CV). The Ag@TiO<SUB>2</SUB> nanocomposite was fabricated on a glassy carbon electrode (GCE) and their electrochemical performance was analyzed by CV, differential pulse voltammetry and electrochemical impedance spectroscopy. The Ag@TiO<SUB>2</SUB> nanocomposite modified GCE (Ag@TiO<SUB>2</SUB>/GCE) displayed excellent performance towards H<SUB>2</SUB>O<SUB>2</SUB> sensing at -0.73V in the linear response range from 0.83μM to 43.3μM, within a detection limit and sensitivity of 0.83μM and ~65.2328+/-0.01μAμM<SUP>-1</SUP>cm<SUP>-2</SUP>, respectively. In addition, Ag@TiO<SUB>2</SUB>/GCE exhibited good operational reproducibility and long term stability.

22 H. pylori 감염시 NSAID의 항암 효과에 대한 실험적 증거

김도현 ( D. H. Kim ),이기명 ( K. M. Lee ),박현진 ( H. J. Park ),최찬임 ( C. I. Chor ),원명이 ( M. I. Won ),김인혜 ( I. H. Kim ),정재연 ( J. H. Jung ),유병무 ( B. M. Yoo ),김진홍 ( J. H. Kim ),조성원 ( S. W. Cho ),함기백 ( K. B. Hahm 대한소화기학회 2002 대한소화기학회 추계학술대회 Vol.2002 No.-

Si film electrodes with surface-modified Cu current collectors for micro Li batteries

Lee, M.j.,Chae, M.r.,Jeong, J.s.,Noh, J.p.,Ahn, H.j.,Cho, K.k.,Choi, H.k.,Nam, T.h.,Kim, K.w.,Cho, G.b. Pergamon Press 2016 Materials research bulletin Vol.82 No.-

<P>Si film electrodes were fabricated onsurface-modified Cu current collectors using an oxidation-reduction process. Flower-like nanostructures (FLNSs) with diameters of 2-3 mu m and plate-like nanostructures (PLNSs) with lengths of 1 m were formed on the Cu foil oxidized at 423 K for 0.5 h, but only the PLNSs remained after sonication. Reduction of the preoxidized Cu foil at 673 K resulted in the formation of platelike and coral -like nanostructures on the Cu foils reduced for 1 and 3 h and a smooth surface without specific structures on the Cu foil reduced for 6 h. The best electrochemical properties in terms of the first columbic efficiency (85.4%) and the cycle performance (67.3% at 50 cycles) were obtained from the Si film electrode fabricated on the Cu foil that had been reduced for 3 h because the coral -like nanostructures on the Cu foil enhanced the adhesion of the Si film and improved the structural stability of the Si film electrode during the electrochemical reactions. (C) 2016 Elsevier Ltd: All rights reserved.</P>

Interface sulfur passivation using H₂S annealing for atomic-layer-deposited Al₂O₃ films on an ultrathin-body In_0.53Ga_0.47As-on-insulator

Jin, H.S.,Cho, Y.J.,Lee, S.M.,Kim, D.H.,Kim, D.W.,Lee, D.,Park, J.B.,Won, J.Y.,Lee, M.J.,Cho, S.H.,Hwang, C.S.,Park, T.J. New York] ; North-Holland 2014 APPLIED SURFACE SCIENCE - Vol.315 No.-

Atomic-layer-deposited Al<SUB>2</SUB>O<SUB>3</SUB> films were grown on ultrathin-body In<SUB>0.53</SUB>Ga<SUB>0.47</SUB>As substrates for III-V compound-semiconductor-based devices. Interface sulfur (S) passivation was performed with wet processing using ammonium sulfide ((NH<SUB>4</SUB>)<SUB>2</SUB>S) solution, and dry processing using post-deposition annealing (PDA) under a H<SUB>2</SUB>S atmosphere. The PDA under the H<SUB>2</SUB>S atmosphere resulted in a lower S concentration at the interface and a thicker interfacial layer than the case with (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment. The electrical properties of the device, including the interface property estimated through frequency dispersion in capacitance, were better for (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment than the PDA under a H<SUB>2</SUB>S atmosphere. They might be improved, however, by optimizing the process conditions of PDA. The PDA under a H<SUB>2</SUB>S atmosphere following (NH<SUB>4</SUB>)<SUB>2</SUB>S wet-treatment resulted in an increased S concentration at the interface, which improved the electrical properties of the devices.